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Search Results: 1 - 10 of 244228 matches for " Carlos Roberto Carvalho;Manh?es-de-Castro "
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Aderência e atividade microbicida de monócitos em portadores de esquistossomose mans nica na forma hepatoesplênica cirúrgica
Brandt Carlos Teixeira,Leite Carlos Roberto Carvalho,Manhes-de-Castro Raul,Brandt Filho Carlos
Acta Cirurgica Brasileira , 2003,
Abstract: A cirurgia nas crian as portadoras de esquistossomose mans nica inclui esplenectomia, ligadura da veia gástrica esquerda e o auto-implante de tecido esplênico no omento maior. A eficácia desse procedimento pode ser responsável pelo desaparecimento da sepse fulminante pós-esplenectomia (SFPE) neste tipo de paciente. Esta condi o é atribuída à diminui o de IgM, de linfócitos circulantes, de properdina e ausência de tuftsina, o que conduz a deficiência da atividade das células macrófágicas, que s o responsáveis pela aderência à bactéria, fagocitose e destrui o das mesmas. OBJETIVO: Analisar os aspectos funcionais dos monócitos destes pacientes, operados quando crian as, no Servi o de Cirurgia Geral da Crian a do Hospital das Clínicas da UFPE, entre 1991 a 2000. MéTODOS: Foram analisados os índices de aderência in vitro dos monócitos e a gera o do anion superóxido (O2-), em três grupos. O 1masculine, auto-implante (AI), constituído por 18 portadores de esquistossomose mans nica na forma hepatoesplênica, submetidos a esplenectomia, ligadura da veia gástrica esquerda e auto-implante de tecido esplênico no omento maior; o 2masculine, (ESP), formado por nove pacientes similares, submetidos a esplenectomia e desconex o ázigo-portal, e o 3masculine,(CT), constituído por 12 adolescentes sadios, oriundos da mesma condi o sócio-econ mica-geográfica. RESULTADOS: N o houve diferen a no índice de aderência entre os três grupos. Os monócitos dos pacientes do grupo AI tiveram a gera o de O2- semelhante à dos indivíduos do grupo CT, e significantemente maior do que os pacientes do grupo ESP. CONCLUS ES: Os monócitos dos portadores de esquistossomose hepatoesplênica submetidos a esplenectomia, ligadura da veia gástrica esquerda e auto-implante de tecido esplênico no omento maior se mostram funcionalmente similares aos de indivíduos normais da mesma condi o sócio-econ mica-geográfica.
Aderência e atividade microbicida de monócitos em portadores de esquistossomose mans?nica na forma hepatoesplênica cirúrgica
Brandt, Carlos Teixeira;Leite, Carlos Roberto Carvalho;Manhes-de-Castro, Raul;Brandt Filho, Carlos;Castro, Célia Maria Machado Barbosa de;
Acta Cirurgica Brasileira , 2003, DOI: 10.1590/S0102-86502003000200011
Abstract: surgical treatment in children suffering from schistosomissis mansoni includes splenectomy; ligature of left gastric vein and auto-implantation of spleen morsels into the greater omentum. the efficacy of this procedure may be responsible for the disappearance of overwhelming post-splenectomy infection (opsi), in this kind of patients. this condition may be assigned to the lowering of igm, circulating lymphocytes, properdin and to the absence of tutfsin, which may lead to the deficiency of mono-macrophage activity cells, responsible for bacteria adherence, phagocytosis and its destruction. purpose: to analyze the functional features of the monocytes from young patients, who underwent splenectomy, ligature of left gastric vein and auto-implantation of morsels of spleen tissue on the major omentum. these patients were cared for at - university hospital - "servi?o de cirurgia geral da crian?a, hospital das clínicas, ufpe", from 1991 to 2000. methods: it was analyzed the rates of monocytes in vitro adherence to the solid surface and the generation of o2- radical by the monocytes, stimulated with acetate miristate of phorbol (pma) and pma + tuftsin, in 3 groups. the 1st group (ai), constituted by 18 patients with schistosomiasis mansoni in the hepatosplenic form and treated with splenectomy, ligature of left gastric vein and auto-implantation of fragments of spleen tissue on the major omentum; the 2nd group (esp), formed by 09 patients with schistosomiasis mansoni in the hepatosplenic form who underwent azigo-portal disconnection with splenectomy and the 3rd one (ct) constituted by 12 voluntary teenagers from the same geographic area and with the same socio-economical conditions. results: there was no significant difference in the monocyte adherence rate among the 3 groups. with regard to the generation of o2- release, the monocytes of patients from ai group had a super oxide generation similar to that of individuals from ct group, and higher than the patients from esp gr
Esquitossomose mans?nica hepatoesplênica humana: produ??o de TNF-alfa em monócitos
Brandt, Carlos Teixeira;Leite, Carlos Roberto Carvalho;de-Castro, Célia M. M. B;Manhes-de-Castro, Raul;Brandt Filho, Carlos;
Revista do Colégio Brasileiro de Cirurgi?es , 2003, DOI: 10.1590/S0100-69912003000600008
Abstract: background: to investigate tnf-a levels released by monocyte culture in bearers of human hepatosplenic schistosomiasis mansoni. methods: in this study we randomly included 39 volunteers with ages from 15 to 31 years, 19 men and 20 women, divided into three groups. control group 1 (cg) 12 healthy people without schistosomiasis. auto implanted spleen group 2 (ais) 18 young adults with hepatosplenic schistosomiasis mansoni who underwent splenectomy, ligature of the left gastric vein and auto implantation of spleen tissue into the major omentum, when the age varied between 7 and 16 years. these patients received oxaminiquine in a single dose of 20mg/kg 30 days before the surgical treatment. the mean follow-up was about 8 years. group 3 - splenectomyzed patients without auto implantation of spleen tissue (sp). nine young adults were included and underwent total splenectomy without auto implantation of spleen tissue and azigo portal disconnection. the patients with schistosomiasis from groups i and iii had the confirmation of their disease by the presence of symmers fibrosis in the wedge liver biopsies during the surgical procedure. six 6ml of blood from all volunteers we collected.. the monocytes were separated by centrifugation and cultivated in cultilabò medium. supernatant samples of 100ml from the monocyte culture (106cells/ml), of each person were taken for the measure of tnf-a concentrations. this measure was performed by colorimetric elisa (quantikinetm - r&d systems), after 4 hours of stimulation with pma and incubation with 37oc humid atmosphere of 5% co2. results: the tnf-a concentrations did not differ significantly among the three groups [(gc 135.0 ± 51.6 pg/ml; ais 97.0 ± 25.4 pg/ml and sp 107.0 ±. 52.1 pg/ml) - anova f = 0.210; p = 0.813]. conclusions: these findings give support to the hypothesis that after splenectomy with or without auto implantation of spleen tissue the monocyte function, as far as the production of tnf-a is concerned, it is still prese
Neonatal exposure to citalopram, a serotonin selective reuptake inhibitor, programs a delay in the reflex ontogeny in rats
Deiró, Teresa Cristina Bomfim de Jesus;Carvalho, Judelita;Nascimento, Elizabeth do;Medeiros, Jaiza Maria Barreto;Cajuhi, Fabiana;Ferraz-Pereira, Kelli Nogueira;Manhes-de-Castro, Raul;
Arquivos de Neuro-Psiquiatria , 2008, DOI: 10.1590/S0004-282X2008000500025
Abstract: serotonin influences the growth and development of the nervous system, as well as its behavioral manifestations. the possibility exists that increased brain serotonin availability in young animals modulates their neuro-behavioral responses. this study investigated the body weight gain and reflex ontogeny of neonatal rats treated during the suckling period with two doses of citalopram (5 mg, or 10 mg/kg, sc, daily). the time of the appearance of reflexes (palm grasp righting, free-fall righting, vibrissa placing, auditory startle response, negative geotaxis and cliff avoidance) as well as the body weight evolution were recorded. in general, a delay in the time of reflex development and a reduced weight gain were observed in drug-treated animals. these findings suggest that serotoninergic mechanisms play a role in modulating body weight gain and the maturation of most reflex responses during the perinatal period in rats.
Efeito do tratamento com triptofano sobre parametros do comportamento alimentar em ratos adultos submetidos à desnutri??o neonatal
Carvalho-Santos, Judelita;Queirós-Santos, Adenilda;Morais, Graciele Lima;Santana, Laila Hohlenwerger Silva;Brito, Monique Gomes;Araújo, Rachel Chagas Silva;Manhes-de-Castro, Raul;Deiró, Tereza Cristina Bomfim de Jesus;Barreto-Medeiros, Jairza Maria;
Revista de Nutri??o , 2010, DOI: 10.1590/S1415-52732010000400001
Abstract: objective: this study investigated the effects of tryptophan on the eating behavior of adult rats submitted or not to early malnutrition. methods: sixty-four male wistar rats were divided into nourished (n=32, casein=17%) and malnourished (n=32, casein=8%) according to the diet given to the dam during lactation. after weaning, all rats were fed a diet with a protein content of 23%. the rats were weighed on day 7, day 21 and day 70 after birth. on day 70 after birth, each nutritional group was divided into 4 subgroups: nourished-saline (n=16), nourished-tryptophan (n=16), malnourished-saline (n=16) and malnourished-tryptophan (n=16). the tryptophan groups were given 1.0ml/100g of tryptophan for 14 days, at a dosage of 50mg/kgw of body weight and the saline groups were given 1.0ml/100g of 0.9% nacl, also for 14 days. the eating behavior parameters were assessed during this period. the mean relative food intake and mean relative weight gain were then determined. the statistical analyses were done by the student's t-test and anova, followed by the tukey test, with p<0.05. results: during the first 70 days of life, pups from protein-malnourished damns remained lighter than pups from well-nourished damns (p<0.01). well-nourished rats treated with tryptophan (m=6.88, sd=0.05) ate less than those given saline (m=7.27, sd=0.08) (p<0.01) but weight was unaffected. no difference was found for the malnourished rats. conclusion: in this study, neonatal protein restriction affected weight gain in rats. furthermore, early malnutrition made adult rats resistant to the inhibitory effects of tryptophan on food intake.
Behavioral satiety sequence: an experimental model for studying feeding behavior
Oliveira, Lisiane dos Santos;Souza, Sandra Lopes de;Manhes-De-Castro, Raul;
Revista de Nutri??o , 2011, DOI: 10.1590/S1415-52732011000400010
Abstract: feeding behavior is controlled by interactions between psychobiological and physiological systems. in rats, there is a sequence in the feeding behavior that is characterized by similar movements at the beginning and end of a meal, known as the behavioral satiety sequence. in the sequence, eating is followed by grooming and other activities, and ends with resting. the objective of this systematic review is to evaluate the use of the behavioral satiety sequence as an experimental model for the study of feeding behavior. a systematic search of the electronic databases medline, lilacs, scielo, cochrane library and pubmed was done from november 2007 to january 2008, using combinations of the keywords "behavioral," "satiety" and "sequence". ninety articles were found and, of these, fifteen articles were selected for the review. the studies demonstrated the efficacy of using behavioral satiety sequence to evaluate the effects of some types of manipulations on feeding behavior. with this study method it was also possible to observe different factors that can interfere with feeding behavior, such as sedation, malaise or intake inhibition, by increasing satiety. behavioral satiety sequence offers solid tools for gaining a better understanding of how treatment can influence feeding behavior.
The expression of an intraspecific aggressive reaction in the face of a stressor agent alters the immune response in rats
Barreto-Medeiros, J. M.;Feitoza, E. G.;Magalh?es, K.;da Silva, R. R.;Manhes-de-Castro, F. M.;Manhes-de-Castro, R.;De-Castro, C. M. M. B.;
Brazilian Journal of Biology , 2005, DOI: 10.1590/S1519-69842005000200003
Abstract: the repercussion on the immune response of the expression of intraspecific aggressiveness in the face of a stressor agent was investigated in rats. ninety-day-old animals were divided into three groups: the control group (only immunological measurements were performed), the foot-shock (fs) (animals individually receiving fs), and the intraspecific aggressive response (iar) group (animals receiving fs and presenting iar). for immunological measurements, blood samples were collected promptly at 7 and 15 days after fs or iar. the fs reduced the total leukocyte amount presented. however, aggressiveness triggered not only reduction of the leukocytes, but also lymphocyte decrease and neutrophil increase. moreover, an elevation in total leukocytes associated with an increase in the humoral immune response was also observed one week after iar. in this study, the expression of intraspecific aggressiveness in the face of a stressor seemed to activate the immune system and to potentiate the antigen specific humoral response.
Efeito do treinamento físico e da desnutri??o durante a gesta??o sobre os eixos cranianos de ratos neonatos
Fidalgo, Marco;Macêdo, érika Michelle Correia de;Dantas, Renata;Foerster, Patrícia;Neves, Caroline;Manhes-de-Castro, Raul;Leandro, Carol Góis;
Revista Brasileira de Medicina do Esporte , 2010, DOI: 10.1590/S1517-86922010000600009
Abstract: in critical periods of body development, environmental stimuli such as physical exercise and diet may influence on placental flow and fetal somatic growth. the aim of this study was to evaluate the effects of physical training and malnutrition during pregnancy on the skull axis of newborn rats. male wistar rats were divided according to manipulation of their mothers: untrained control (cf, n = 25), trained (tf, n = 25), untrained and malnourished (mf, n = 25), trained and malnourished (tmf, n = 25). trained mothers (t and tm) were submitted to 8 weeks of moderate physical training before and during pregnancy (60min/day, 5 days/wk to 65% of vo2max). malnourished mothers (m and tm) received a low protein diet during pregnancy (8% casein) while the nourished (c and t) were fed with normal diet (17% casein). on the 1st postnatal day, the number of pups born per litter, litter weight and birth weight, latero-lateral axis of skull (llas) and antero-posterior axis of skull (apas), longitudinal axis of the body (la) and length of tail (lt) of each neonate were verified. on the 3rd day after delivery, the brains were extracted and weighed. during pregnancy, the females of the t and m groups showed lower weight gain compared with group c at 3rd week (c = 34.4 ± 1.3, t = 30.7 ± 0.60, m = 25.8 ± 0.78, tm = 29.9 ± 0.83). the malnourished group had lower birth weight in relation to their respective controls (cf = 6.3 ± 0.1, tf = 6.3 ± 0.1, mf = 4.7 ± 0.07; tmf = 5.0 ± 0.06). group mf presented measures of llas (cf = 9.8 ± 0.06, tf = 9.8 ± 0.05, mf = 9.2 ± 0.04; tmf = 9.6 ± 0.13) and eapc (cf = 18.1 ± 0.1, tf = 18.2 ± 0.1, mf = 17.5 ± 0.1, tmf = 18.0 ± 0.2) lower compared to controls. based on the results, it can be concluded that malnutrition during fetal period changed somatic development, while physical training positively influenced on the skull axis of the concepts.
Neonatal administration of citalopram delays somatic maturation in rats
Deiró T.C.B.J.,Manhes-de-Castro R.,Cabral-Filho J.E.,Souza S.L.
Brazilian Journal of Medical and Biological Research , 2004,
Abstract: We investigated the somatic maturation of neonate rats treated during the suckling period with citalopram, a selective serotonin reuptake inhibitor. Groups with 6 male neonates were randomly assigned to different treatments 24 h after birth. Each litter was suckled by one of the dams until the 21st postnatal day. Body weight, head axis and tail length were measured daily from the 1st to the 21st postnatal day. Time of ear unfolding, auditory conduit opening, incisor eruption, and eye opening was determined. Pups received 5 mg (Cit5), 10 mg (Cit10) or 20 mg/kg (Cit20) citalopram sc, or saline (0.9% NaCl, w/v, sc). Compared to saline, body weight was lower (24.04%, P < 0.01) for Cit10 from the 10th to the 21st day and for Cit20 from the 6th to the 21st day (38.19%, P < 0.01). Tail length was reduced in the Cit20 group (15.48%, P < 0.001) from the 8th to the 21st day. A reduction in mediolateral head axis (10.53%, P < 0.05) was observed from the 11th to the 21st day in Cit10 and from the 6th to the 21st day in Cit20 (13.16%, P < 0.001). A reduction in anteroposterior head axis was also observed in the Cit20 group (5.28%, P < 0.05) from the 13th to the 21stday. Conversely, this axis showed accelerated growth from the 12th to the 21stday in the Cit5 group (13.05%, P < 0.05). Auditory conduit opening was delayed in the Cit5 and Cit20 groups and incisor eruption was delayed in all citalopram groups. These findings show that citalopram injected during suckling to rats induces body alterations and suggest that the activity of the serotoninergic system participates in growth mechanisms.
Efeitos do tratamento neonatal com inibidor seletivo de recaptura da 5-HT sobre o desenvolvimento anat?mico cranio-encefálico
Magalh?es, Carolina Peixoto;Lima, Luciene Oliveira de;Silva, Matilde Cesiana da;Marinho, S?nia Maria Oliveira Cavalcanti;Nascimento, Elizabeth do;Silva, Cristiano Mendes da;Souza, Sandra Lopes de;Manhes-de-Castro, Raul;
Arquivos de Neuro-Psiquiatria , 2006, DOI: 10.1590/S0004-282X2006000600019
Abstract: neonatal repercussion researched of the serotonin selective recapture inibitor (ssri) chronic treatment about the somatic growth, of the encephalon and skull. male rats were divided into groups: control (nacl) and cit (10 μl/kg citalopram 10 mg). in 21 post birth days were measured body weight, side axle , front and rear and skull circle. at 8, 15, 22 days after birth, animals were sacrified for the encephalon withdrawal to evaluate the measurements mentioned above. ssri use caused body growth deficit, skull and encephalon reduction. the retard is possibly caused by the magnitude change of the trophic serotonin action over the skull-facial morphogenesis, reinforcing the serotoninergic system participation over the somatic and ontogenic growth. the ssri possible hypophagic effects are not discarded.
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