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Search Results: 1 - 10 of 229715 matches for " Carlos Jorge Logullo de; "
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Altera??es dos níveis séricos de creatinina, ácido úrico, creatina kinase e da taxa de filtra??o glomerular em corredores de "rua"
Morales, Anderson Pontes;Maciel, Ronaldo Nascimento;Jorge, Felipe Sampaio;Arêas Neto, Nilo Terra;Cordeiro, Daniela de Carvalho;Viana, Maria Auxiliadora Siqueira;Oliveira, Carlos Jorge Logullo de;
Revista Brasileira de Cineantropometria & Desempenho Humano , 2013, DOI: 10.5007/1980-0037.2013v15n1p71
Abstract: the strategies adopted by corridors "street" during the evidence from interference of the race distance and levels of technical and physical competition. the objective of this study was to examine the biochemical effects of creatinine (c), uric acid (au), creatine kinase (ck) and glomerular filtration rate (gfr) caused by a test run of "street" of 6 (six) km participated n=(15) male athletes (40.53 ± 8.65 years) divided into three groups: group 1 best times (g1mt) n = 5, group 2 intermediate times (g2ti) n = 5; group 3 times worst (g3pt) n = 5. blood samples were collected 30 min before and immediately after the race. data were analyzed by two-way anova, wilcoxon and mann whitney test. it was considered significant levels (p<0.05). the results showed that there were significant increases in serum activities of intra-group (c) in g1mt before: 1,18±0,04 mg.dl-1 after: 1.60±0.15 mg.dl-1; g2ti before: 1,04±0,15 mg.dl-1 after: 1,56±0,21 mg.dl-1; g3pt before: 1,08±0,13 mg.dl-1 after: 1,52±0,32 mg.dl-1 and (au) g1mt before: 3,80±0,75 mg.dl-1 after: 4,56±0,94 mg.dl-1; g2ti before: 4,36±1,62 mg.dl-1 after: 5,0±1,69 mg.dl-1; g3pt before: 4,62±1,08 mg.dl-1 after: 5,42±0,86 mg.dl-1, while (ck) and (gfr) showed no significant difference.
Glycogen and Glucose Metabolism Are Essential for Early Embryonic Development of the Red Flour Beetle Tribolium castaneum
Amanda Fraga, Lupis Ribeiro, Mariana Lobato, Vitória Santos, José Roberto Silva, Helga Gomes, Jorge Luiz da Cunha Moraes, Jackson de Souza Menezes, Carlos Jorge Logullo de Oliveira, Eldo Campos, Rodrigo Nunes da Fonseca
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0065125
Abstract: Control of energy metabolism is an essential process for life. In insects, egg formation (oogenesis) and embryogenesis is dependent on stored molecules deposited by the mother or transcribed later by the zygote. In oviparous insects the egg becomes an isolated system after egg laying with all energy conversion taking place during embryogenesis. Previous studies in a few vector species showed a strong correlation of key morphogenetic events and changes in glucose metabolism. Here, we investigate glycogen and glucose metabolism in the red flour beetle Tribolium castaneum, an insect amenable to functional genomic studies. To examine the role of the key enzymes on glycogen and glucose regulation we cloned and analyzed the function of glycogen synthase kinase 3 (GSK-3) and hexokinase (HexA) genes during T. castaneum embryogenesis. Expression analysis via in situ hybridization shows that both genes are expressed only in the embryonic tissue, suggesting that embryonic and extra-embryonic cells display different metabolic activities. dsRNA adult female injection (parental RNAi) of both genes lead a reduction in egg laying and to embryonic lethality. Morphological analysis via DAPI stainings indicates that early development is impaired in Tc-GSK-3 and Tc-HexA1 RNAi embryos. Importantly, glycogen levels are upregulated after Tc-GSK-3 RNAi and glucose levels are upregulated after Tc-HexA1 RNAi, indicating that both genes control metabolism during embryogenesis and oogenesis, respectively. Altogether our results show that T. castaneum embryogenesis depends on the proper control of glucose and glycogen.
Identification and Structural-Functional Analysis of Cyclin-Dependent Kinases of the Cattle Tick Rhipicephalus (Boophilus) microplus
Helga Gomes, Nelilma C. Romeiro, Gloria R. C. Braz, Eduardo Alves Gamosa de Oliveira, Camilla Rodrigues, Rodrigo Nunes da Fonseca, Naftaly Githaka, Masayoshi Isezaki, Satoru Konnai, Kazuhiko Ohashi, Itabajara da Silva Vaz, Carlos Logullo, Jorge Moraes
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0076128
Abstract: Cyclin-dependent kinases (CDKs) are a family of serine/threonine kinases essential for cell cycle progression. Herein, we describe the participation of CDKs in the physiology of Rhipicephalus microplus, the southern cattle tick and an important disease vector. Firstly, amino acid sequences homologous with CDKs of other organisms were identified from a R. microplus transcriptome database in silico. The analysis of the deduced amino acid sequences of CDK1 and CDK10 from R. microplus showed that both have caspase-3/7 cleavage motifs despite their differences in motif position and length of encoded proteins. CDK1 has two motifs (DKRGD and SAKDA) located opposite to the ATP binding site while CDK10 has only one motif (SLLDN) for caspase 3–7 near the ATP binding site. Roscovitine (Rosco), a purine derivative that inhibits CDK/cyclin complexes by binding to the catalytic domain of the CDK molecule at the ATP binding site, which prevents the transfer of ATP's γphosphoryl group to the substrate. To determine the effect of Rosco on tick CDKs, BME26 cells derived from R. microplus embryo cells were utilized in vitro inhibition assays. Cell viability decreased in the Rosco-treated groups after 24 hours of incubation in a concentration-dependent manner and this was observed up to 48 hours following incubation. To our knowledge, this is the first report on characterization of a cell cycle protein in arachnids, and the sensitivity of BME26 tick cell line to Rosco treatment suggests that CDKs are potential targets for novel drug design to control tick infestation.
Germ band retraction as a landmark in glucose metabolism during Aedes aegypti embryogenesis
Wagner Vital, Gustavo Rezende, Leonardo Abreu, Jorge Moraes, Francisco JA Lemos, Itabajara Vaz, Carlos Logullo
BMC Developmental Biology , 2010, DOI: 10.1186/1471-213x-10-25
Abstract: Glucose metabolism was investigated throughout Aedes aegypti (Diptera) embryonic development. Both cellular blastoderm formation (CBf, 5 h after egg laying - HAE) and germ band retraction (GBr, 24 HAE) may be considered landmarks regarding glucose 6-phosphate (G6P) destination. We observed high levels of glucose 6-phosphate dehydrogenase (G6PDH) activity at the very beginning of embryogenesis, which nevertheless decreased up to 5 HAE. This activity is correlated with the need for nucleotide precursors generated by the pentose phosphate pathway (PPP), of which G6PDH is the key enzyme. We suggest the synchronism of egg metabolism with carbohydrate distribution based on the decreasing levels of phosphoenolpyruvate carboxykinase (PEPCK) activity and on the elevation observed in protein content up to 24 HAE. Concomitantly, increasing levels of hexokinase (HK) and pyruvate kinase (PK) activity were observed, and PEPCK reached a peak around 48 HAE. Glycogen synthase kinase (GSK3) activity was also monitored and shown to be inversely correlated with glycogen distribution during embryogenesis.The results herein support the hypothesis that glucose metabolic fate changes according to developmental embryonic stages. Germ band retraction is a moment that was characterized as a landmark in glucose metabolism during Aedes aegypti embryogenesis. Furthermore, the results also suggest a role for GSK3 in glycogen balance/distribution during morphological modifications.The mosquito Aedes aegypti is vector of urban yellow fever and also the main dengue vector [1]. One of the major problems involving dengue transmission is that A. aegypti embryos enter dormancy at the end of embryogenesis, surviving and remaining viable for several months inside the egg [2-4]. This extended viability is possible due to the acquisition of embryonic desiccation resistance, a biological mechanism that is believed to involve the formation and maturation of serosal cuticle, a layer covering the embryo [5]. De
Inhibition of Enzyme Activity of Rhipicephalus (Boophilus) microplus Triosephosphate Isomerase and BME26 Cell Growth by Monoclonal Antibodies
Luiz Saramago,Mariana Franceschi,Carlos Logullo,Aoi Masuda,Itabajara da Silva Vaz,Sandra Estrazulas Farias,Jorge Moraes
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms131013118
Abstract: In the present work, we produced two monoclonal antibodies (BrBm37 and BrBm38) and tested their action against the triosephosphate isomerase of Rhipicephalus ( Boophilus) microplus (RmTIM). These antibodies recognize epitopes on both the native and recombinant forms of the protein. rRmTIM inhibition? by BrBm37 was up to 85% whereas that of BrBrm38 was 98%, depending on the antibody-enzyme ratio. RmTIM activity was lower in ovarian, gut, and fat body tissue extracts treated with BrBm37 or BrBm38 mAbs. The proliferation of the embryonic tick cell line (BME26) was inhibited by BrBm37 and BrBm38 mAbs. In summary, the results reveal that it is possible to interfere with the RmTIM function using antibodies, even in intact cells.
A Mitochondrial Membrane Exopolyphosphatase Is Modulated by, and Plays a Role in, the Energy Metabolism of Hard Tick Rhipicephalus (Boophilus) microplus Embryos
Eldo Campos,Arnoldo R. Fa?anha,Evenilton P. Costa,Amanda Fraga,Jorge Moraes,Itabajara Da Silva Vaz Jr.,Aoi Masuda,Carlos Logullo
International Journal of Molecular Sciences , 2011, DOI: 10.3390/ijms12063525
Abstract: The physiological roles of polyphosphates (polyP) recently found in arthropod mitochondria remain obscure. Here, the relationship between the mitochondrial membrane exopolyphosphatase (PPX) and the energy metabolism of hard tick Rhipicephalus microplus embryos are investigated. Mitochondrial respiration was activated by adenosine diphosphate using polyP as the only source of inorganic phosphate (P i) and this activation was much greater using polyP 3 than polyP 15. After mitochondrial subfractionation, most of the PPX activity was recovered in the membrane fraction and its kinetic analysis revealed that the affinity for polyP 3 was 10 times stronger than that for polyP 15. Membrane PPX activity was also increased in the presence of the respiratory substrate pyruvic acid and after addition of the protonophore carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. Furthermore, these stimulatory effects disappeared upon addition of the cytochrome oxidase inhibitor potassium cyanide and the activity was completely inhibited by 20 μg/mL heparin. The activity was either increased or decreased by 50% upon addition of dithiothreitol or hydrogen peroxide, respectively, suggesting redox regulation. These results indicate a PPX activity that is regulated during mitochondrial respiration and that plays a role in adenosine-5’-triphosphate synthesis in hard tick embryos.
The Modulation of the Symbiont/Host Interaction between Wolbachia pipientis and Aedes fluviatilis Embryos by Glycogen Metabolism
Mariana da Rocha Fernandes, Renato Martins, Evenilton Pessoa Costa, Etiene Casagrande Pacid?nio, Leonardo Araujo de Abreu, Itabajara da Silva Vaz, Luciano A. Moreira, Rodrigo Nunes da Fonseca, Carlos Logullo
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0098966
Abstract: Wolbachia pipientis, a maternally transmitted bacterium that colonizes arthropods, may affect the general aspects of insect physiology, particularly reproduction. Wolbachia is a natural endosymbiont of Aedes fluviatilis, whose effects in embryogenesis and reproduction have not been addressed so far. In this context, we investigated the correlation between glucose metabolism and morphological alterations during A. fluviatilis embryo development in Wolbachia-positive (W+) and Wolbachia-negative (W?) mosquito strains. While both strains do not display significant morphological and larval hatching differences, larger differences were observed in hexokinase activity and glycogen contents during early and mid-stages of embryogenesis, respectively. To investigate if glycogen would be required for parasite-host interaction, we reduced Glycogen Synthase Kinase-3 (GSK-3) levels in adult females and their eggs by RNAi. GSK-3 knock-down leads to embryonic lethality, lower levels of glycogen and total protein and Wolbachia reduction. Therefore, our results suggest that the relationship between A. fluviatilis and Wolbachia may be modulated by glycogen metabolism.
Three-Dimensional Shear Wave Velocity Structure of the Northeastern Brazilian Lithosphere  [PDF]
Jorge Luis de Souza, Newton Pereira dos Santos, Carlos da Silva Vilar
International Journal of Geosciences (IJG) , 2016, DOI: 10.4236/ijg.2016.76063
Abstract: A large number of Rayleigh wave dispersion curves recorded at twenty three seismic stations was used to investigate the 3-D shear wave velocity structure of the northeastern Brazilian lithosphere. A simple procedure to generate a three-dimensional image of Mohorovicic; discontinuity was applied in northeastern Brazil and the Moho 3-D image was in agreement with several isolated crustal thicknesses obtained with different geophysical methods. A detailed 3-D S wave velocity model is proposed for the region. In the crust, our model is more realist than CRUST2.0 global model, because it shows more details either laterally or in depth than global model, i.e., clear lateral variation and gradual increase of S wave velocity in depth. Down to 100 km depth, the 3-D S wave velocity model in northeastern Brazil is dominated by low velocities and this is consistent either with heat flow measurements or with measurements of the flexural strength of the lithosphere developed in the South American continent. Our 3-D S wave velocity model was also used to obtain the lithosphere thickness in each cell of the northeastern Brazil and the results were consistent with global studies about the Lithosphere-Asthenosphere Boundary worldwide.
Cirurgia metabólica: cura para diabete tipo 2
Zeve, Jorge Luiz de Mattos;Tomaz, Carlos Alberto Bezerra;
ABCD. Arquivos Brasileiros de Cirurgia Digestiva (S?o Paulo) , 2011, DOI: 10.1590/S0102-67202011000400012
Abstract: introduction: type 2 diabetes is a metabolic disease characterized by chronic hyperglycemia associated with several organs damage and failure. it is the most common cause of blindness, amputation and kidney failure in western adults, besides increase the risk of coronary disease and stroke with high mortality of patients. the association of type 2 diabetes with obesity is really significant. obese diabetic patients had a better glucose control after bariatric surgery even prior to weight loss. methods: a systematic review in pubmed, bireme and scielo research sites was made, using "diabetes mellitus", "bariatric surgery" and "obesity" keywords. were selected the studies related to surgical techniques in the treatment of obesity and type 2 diabetes. the papers presented at the 1st and 2nd world congress of interventional therapies for type 2 diabetes was also included. conclusion: surgical treatment of patients with obesity and type 2 diabetes showed important initial results controling blood glucose. however, the procedures and techniques used must be more investigated in randomized controlled trials comparing the clinical and surgical treatments, in animals and in humans.
Doen?as granulomatosas ocupacionais
Bagatin, Ericson;Pereira, Carlos Alberto de Costa;Afiune, Jorge Barros;
Jornal Brasileiro de Pneumologia , 2006, DOI: 10.1590/S1806-37132006000800012
Abstract: a variety of diseases are encompassed in the didactic denomination of "granulomatous diseases of probable occupational etiology". as well as presenting similar clinical aspects, such diseases are characterized by certain common traits: formation of granulomas; systemic and respiratory manifestations; environmental or occupational exposure to organic or inorganic agents; and t lymphocyte involvement in the pathogenesis. included in this category are hypersensitivity pneumonitis, mycobacteriosis (all forms) and sarcoidosis, as well as beryllium disease and other lung diseases caused by exposure to heavy metals. in order to highlight the risk of developing one of these diseases as a result of environmental or occupational exposure to etiologic agents, we address aspects related to epidemiology, pathogenesis and evaluation of exposure of these diseases, as well as those related to diagnostic criteria, prevention and control. we have given special emphasis to groups of individuals considered to be at high risk for developing these diseases, as well as to the need for health care professionals to remain aware of the potential occupational etiology of such diseases, a decisive factor in devising effective measures of prevention and epidemiological surveillance.
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