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Search Results: 1 - 10 of 70222 matches for " Carlos Augusto Rosa "
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Impact of a Non-Saccharomyces Yeast Isolated in the Equatorial Region in the Acceptance of Wine Aroma  [PDF]
Mariana Oliveira Assis, Adriana Pereira Coelho Santos, Carlos Augusto Rosa, Maria Eugênia de Oliveira Mamede
Food and Nutrition Sciences (FNS) , 2014, DOI: 10.4236/fns.2014.59086
Abstract:

The aim of this work was to isolate and identify the yeasts prevalent in fresh grapes cultivated in the “São Francisco Valley” region (Brazil), as well as evaluating the cell growth of these indigenous yeasts during the fermentation of grape musts and their contribution to the improvement of wine aroma. The Chenin Blanc grape must fermented by H. opuntiae presented higher acceptance means at the three points analyzed (6.74, 6.78 and 7.30) and in the fermentation carried out by the yeasts H. opuntiae and S. cerevisiae, the highest mean acceptance (7.22) was observed after 120 hours, with no statistical difference from the sample fermented by H. opuntiae alone. Since these samples that showed higher acceptance means also receiving higher scores for purchasing intention, corresponding to the concepts of “definitely would buy” and “probably would buy”. The present study suggests that the fermentations of grape musts carried out by the yeast H. opuntiae and mixed cultures of H. opuntiae and Saccharomyces cerevisiae, positively influenced the sensory qualities of the wines and showed greater potential to increase the aroma of the musts and to develop specific wine styles.

Screening of Brazilian basidiomycetes for antimicrobial activity
Rosa, Luiz Henrique;Machado, Kátia M Gomes;Jacob, Camila Cristina;Capelari, Marina;Rosa, Carlos Augusto;Zani, Carlos Leomar;
Memórias do Instituto Oswaldo Cruz , 2003, DOI: 10.1590/S0074-02762003000700019
Abstract: a total of 103 isolates of basidiomycetes, representing 84 species from different brazilian ecosystems, were evaluated for their antifungal and antibacterial activity in a panel of pathogenic and non-pathogenic microorganisms. tissue plugs of the fruiting bodies were cultivated in liquid media and the whole culture extracted with ethyl acetate. crude extracts from agaricus cf. nigrecentulus, agrocybe perfecta, climacodon pulcherrimus, gloeoporus thelephoroides, hexagonia hydnoides, irpex lacteus, leucoagaricus cf. cinereus, marasmius cf. bellus, marasmius sp., nothopanus hygrophanus, oudemansiella canarii, pycnoporus sanguineus, phellinus sp., and tyromyces duracinus presented significant activity against one or more of the target microorganisms. eight isolates were active only against bacteria while three inhibited exclusively the growth of fungi. two extracts presented wide antimicrobial spectrum and were active against both fungi and bacteria. differences in the bioactivity of extracts obtained from isolates from the same species were observed.
Yeasts Occurring in Surface and Mouth Cavity of Two Chelonian Species, Podocnemis expansa Schweigger and P. unifilis Troschel (Reptilia: Chelonia: Pelomedusidae), in the Javaés River Border of Araguaia National Park in Brazil
Paula Benevides de Morais,Raphael Sanzio Pimenta,Inara Brito Tavares,Virginia de Garcia,Carlos Augusto Rosa
International Journal of Microbiology , 2010, DOI: 10.1155/2010/504524
Abstract: Thirty-eight specimens of free-ranging Podocnemis expansa (Amazon turtle) and 22 of P. unifilis (Tracajá) were screened for yeast isolation from surface (plastron, skin, and nails), eye, and mouth cavity. A hundred and eighteen yeast isolates belonging to 39 species were obtained. Debaryomyces hansenii, Candida galli, C. sake, and Rhodotorula mucilaginosa were the most frequent species isolated from these chelonians. Species diversity measured by Shannon's index was shown to be low and a degree of dominance could be detected as species known as potential pathogens were commonly isolated. The effective number of species in plastron of P. expansa was higher than in mouth samples, but not in P. unifilis probably due to dietary factors. P. expansa animals were captured on the beaches, and the superficial yeast populations may include terrestrial species. P. unifilis animals were captured in the water and the yeasts from superficial sites may represent species from river water.
Trophic state and microorganisms community of major sub-basins of the middle Rio Doce basin, southeast Brazil
Petrucio, Mauricio Mello;Medeiros, Adriana Oliveira;Rosa, Carlos Augusto;Barbosa, Francisco Ant?nio Rodrigues;
Brazilian Archives of Biology and Technology , 2005, DOI: 10.1590/S1516-89132005000500015
Abstract: total phosphorus concentration was used to define the trophic state of the main sub-basins of the middle rio doce, in minas gerais state (southeast brazil) and physical, chemical, and microbiological variables of water were analyzed during 2000 to 2001. the study evaluated changes in water quality caused by seasonality and human activities. water temperature, conductivity, ph, total alkalinity, dissolved oxygen, and concentrations of nh4-n, no2-n, no3-n, total-n, po4-p, total-p, doc, and chlorophyll-a were measured in seven rivers stretches (sampling stations). total yeasts, faecal and total coliforms, and heterotrophic bacteria were also determined. the studied areas were considered to be under oligotrophic to eutrophic conditions. the variables that presented highest positive correlation with faecal coliforms were total-p and total-n, and heterotrophic bacteria density was identified as a good parameter to differentiate the ecosystems. these results suggested the inclusion of the trophic level and the distinct activities within a watershed as important elements when proposing conservation and restoration areas.
Fatores preditores para o óbito neonatal em gesta??es com diástole zero ou reversa na doplervelocimetria da artéria umbilical
Martins Neto, Manoel;Carvalho, Francisco Herlanio Costa;Mota, Rosa Maria Salani;Alencar Júnior, Carlos Augusto;
Revista Brasileira de Ginecologia e Obstetrícia , 2010, DOI: 10.1590/S0100-72032010000700008
Abstract: purpose: to evaluate the antenatal and postnatal risk factors of neonatal death in pregnancies with absent (dz) or reverse (dr) end-diastolic flow in the umbilical artery. methods: a cross-sectional retrospective study based on data from 48 medical records of singleton pregnancies with dz or dr, and gestational age of 24 to 34 weeks, at a maternity in the brazilian northeast. mean age was 27.3 (sd: 7.9) years. twenty (41.7%) patients were primiparas. hypertensive disorders were found in 44 (91.7%) cases. thirty-five women (72.9%) had dz and 13 (27.1%) had dr. univariate analysis was firstly done (student's t-test and fisher's exact test) correlating the parameters with the assessed outcome (neonatal death). variables that showed significant association were included in the logistic regression model (wald statistics). the level of significance was set at 5%. results: the perinatal mortality rate was 64.6% (31/48). there were five stillbirths and 26 neonatal deaths. the mean gestational age at diagnosis was 27.9 (sd: 2.8) weeks. deliveries before 24 hours after diagnosis occurred in 52.1% of the cases. cesarean section was performed in 85.4% of the sample. the newborns weighed 975.9 g on average (sd: 457.5). twenty-four (57.1%) presented apgar scores below 7 in the first minute and 21.4% in the fifth minute. gestational age at diagnosis, birth weight and apgar of the first minute proved to be variables significantly related to neonatal death (p values were: 0.008, 0.004, and 0.020, respectively). the odds ratio was 6.6, 25.3 and 13.8 for neonatal death, when the diagnosis was established at the 28th week, weight was <1000 g and first minute apgar score was <7, respectively. conclusions: gestational age at diagnosis, birth weight and apgar score at the first minute were factors that could predict neonatal death in pregnancies with dv or dr determined by umbilical artery doppler velocimetry.
Genetic divergence and parent selection of sugarcane clones
Valéria Rosa Lopes,Jo?o Carlos Bespalhok Filho,Ricardo Augusto de Oliveira,Edson Perez Guerra
Crop Breeding and Applied Biotechnology , 2008,
Abstract: The objective of this study was to estimate the genetic divergence of 140 sugarcane clones of the series RB97,in phase T3 of the Sugarcane Genetic Improvement Program of the Universidade Federal do Paraná, at three locations bymultivariate analysis, using the linear mixed model and grouping analysis by the Tocher procedure, based on Mahalanobis′generalized distance. The evaluated traits were number of stalks per plot, mass of ten stalks, Brix and Brix per plot in kg. Thenumber of groups varied according to the evaluated environment. Based on the results, combinations of one of the mostdivergent clones RB975008, RB975112, RB975019 RB975153 and RB975067 with any one of the most productive clonesRB975269, RB977533, RB975102, RB975317 and RB975038 are recommended.
Mediastinite descendente necrosante pós-angina de Ludwig
BROMMELSTROET MARICéLIA,ROSA JOSé FIORAVANTE TOSATTI DA,BOSCARDIM PAULO CéSAR BUFFARA,SCHMIDLIN CARLOS AUGUSTO
Jornal de Pneumologia , 2001,
Abstract: A angina de Ludwig é uma infec o do espa o submandibular originada, em geral, da infec o do 2o ou 3o molar inferior. Como conseqüência, pode causar mediastinite descendente necrosante, que representa uma forma grave e rara de infec o mediastinal, a qual exige diagnóstico precoce e tratamento cirúrgico para reduzir a alta mortalidade associada a esta doen a. Dois casos de mediastinite descendente necrosante pós-angina de Ludwig foram tratados com excelentes resultados em nosso hospital. A drenagem mediastinal transcervical está justificada em pacientes com doen a limitada ao mediastino superior. Porém, sepse com comprometimento extenso do mediastino requer drenagem através de toracotomia sem demora.
Yeasts Occurring in Surface and Mouth Cavity of Two Chelonian Species, Podocnemis expansa Schweigger and P. unifilis Troschel (Reptilia: Chelonia: Pelomedusidae), in the Javaés River Border of Araguaia National Park in Brazil
Paula Benevides de Morais,Raphael Sanzio Pimenta,Inara Brito Tavares,Virginia de Garcia,Carlos Augusto Rosa
International Journal of Microbiology , 2010, DOI: 10.1155/2010/504524
Abstract: Thirty-eight specimens of free-ranging Podocnemis expansa (Amazon turtle) and 22 of P. unifilis (Tracajá) were screened for yeast isolation from surface (plastron, skin, and nails), eye, and mouth cavity. A hundred and eighteen yeast isolates belonging to 39 species were obtained. Debaryomyces hansenii, Candida galli, C. sake, and Rhodotorula mucilaginosa were the most frequent species isolated from these chelonians. Species diversity measured by Shannon's index was shown to be low and a degree of dominance could be detected as species known as potential pathogens were commonly isolated. The effective number of species in plastron of P. expansa was higher than in mouth samples, but not in P. unifilis probably due to dietary factors. P. expansa animals were captured on the beaches, and the superficial yeast populations may include terrestrial species. P. unifilis animals were captured in the water and the yeasts from superficial sites may represent species from river water. 1. Introduction According to Summerbell [1], an important component of the fungal biodiversity of any given area occurs in habitats defined or conditioned primarily by vertebrates. Such habitats include the animals themselves which are colonized by commensals and disease-causing fungi, as well as organic materials making up dwelling places of those animals. Jones et al. [2] have studied fungi occurring in fecal samples of the Eastern box turtle (Terrapene carolina carolina), a facultative mycovore reptile that may play an important role in fungal spore dispersion. These authors isolated two yeasts, Cryptococcus albidus and Rhodotorula mucilaginosa, that are reported to naturally occur on Trifolium seeds found in fecal samples. Pathogenic interactions are mostly opportunistic [3] and yeasts present a variety of hosts [4–9]). Kostka et al. [10] did postmortem examination in 91 reptiles that revealed that the intestines of 80.6% of the animals carried yeasts. The authors found 56 yeast isolates belonging to the genera Candida (39), Trichosporon (13), Torulopsis (9), and Rhodotorula (3), and one nonidentified teleomorph yeast species. However, they point that no sufficiently reliable criteria could be established to prove that yeasts are associated with disease in reptiles. Erosion and traumatic lesions are common in scutelum and plastron of aquatic turtles that may be caused by algae, bacteria, and fungi [11]. We studied the yeasts occurring in Podocnemis expansa (Amazon turtle) and Podocnemis unifilis (Tracajá), reptiles of Testudines family that occur in the rivers of Araguaia Plains,
Distribution of the endophytic fungi community in leaves of Bauhinia brevipes (Fabaceae)
Hilarino, Mariana Patrícia Amorim;Silveira, Fernando Augusto de Oliveira e;Oki, Yumi;Rodrigues, Leonardo;Santos, Jean Carlos;Corrêa Junior, Ary;Fernandes, Geraldo Wilson;Rosa, Carlos Augusto;
Acta Botanica Brasilica , 2011, DOI: 10.1590/S0102-33062011000400008
Abstract: endophytic fungi represent large, yet unexplored components of biodiversity. this work evaluated the richness and the distribution of endophytes in the leaves of bauhinia brevipes (fabaceae). a total of 1110 colonies were recovered from the samples and grouped by their morphological traits into 126 taxa. the total number of taxa according to leaf development was: 102 in mature leaves, 93 in recently expanded leaves and 79 for unfolded leaves. the major endophyte genera were phomopsis, followed by dothiorella, pestalotiopsis and acremonium. the richness and the isolate numbers of endophytes were not statistically affected by leaf region. however, some taxa were leaf-age specific; six were isolated only from unfolded leaves, nine from recently expanded leaves and 17 were exclusively found in mature leaves. the composition of endophytes varied with leaf region; the similarities (jaccard's index) among the leaf regions of different leaf ages ranged from 0.36 to 0.46, indicating a high spatial variation in the community of endophytic fungi inside the leaves. the high richness of endophytes in this host plant highlights a significant contribution of fungi to tropical biodiversity and the need for further research in this area.
Leishmanicidal Metabolites from Cochliobolus sp., an Endophytic Fungus Isolated from Piptadenia adiantoides (Fabaceae)
Fernanda Fraga Campos,Luiz Henrique Rosa,Betania Barros Cota,Rachel Basques Caligiorne,Ana Lúcia Teles Rabello,Tania Maria Almeida Alves,Carlos Augusto Rosa,Carlos Leomar Zani
PLOS Neglected Tropical Diseases , 2008, DOI: 10.1371/journal.pntd.0000348
Abstract: Protozoan parasites belonging to genera Leishmania and Trypanosoma are the etiological agents of severe neglected tropical diseases (NTDs) that cause enormous social and economic impact in many countries of tropical and sub-tropical areas of the world. In our screening program for new drug leads from natural sources, we found that the crude extract of the endophytic fungus Cochliobolus sp. (UFMGCB-555) could kill 90% of the amastigote-like forms of Leishmania amazonensis and inhibit by 100% Ellman's reagent reduction in the trypanothione reductase (TryR) assay, when tested at 20 μg mL?1. UFMGCB-555 was isolated from the plant Piptadenia adiantoides J.F. Macbr (Fabaceae) and identified based on the sequence of the internally transcribed spacer (ITS) regions of its ribosomal DNA. The chromatographic fractionation of the extract was guided by the TryR assay and resulted in the isolation of cochlioquinone A and isocochlioquinone A. Both compounds were active in the assay with L. amazonensis, disclosing EC50 values (effective concentrations required to kill 50% of the parasite) of 1.7 μM (95% confidence interval = 1.6 to 1.9 μM) and 4.1 μM (95% confidence interval = 3.6 to 4.7 μM), respectively. These compounds were not active against three human cancer cell lines (MCF-7, TK-10, and UACC-62), indicating some degree of selectivity towards the parasites. These results suggest that cochlioquinones are attractive lead compounds that deserve further investigation aiming at developing new drugs to treat leishmaniasis. The findings also reinforce the role of endophytic fungi as an important source of compounds with potential to enter the pipeline for drug development against NTDs.
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