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Search Results: 1 - 10 of 454869 matches for " Carlos Alexandre;Müller "
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Journal of the Chilean Chemical Society , 2003,
Abstract: The essential oils obtained from the dried and fresh leaves of Duguetia glabriuscula - (Annonaceae) were analyzed by GC/MS and compared. By employing the usual phytochemical workup, five aromadendrane sesquiterpenes were isolated from the essential oil obtained from fresh leaves: alloaromadendran-14beta-al, alloaromadendrene, (-)-ledol, viridiflorol, and (+)-spathulenol. Alloaromadendran-14beta-oic acid was also obtained, having been generated by the spontaneous oxidation of its corresponding aldehyde. Both essential oils displayed activity against Artemia salina Los aceites esenciales obtenidos de hojas secas y frescas de Duguetia glabriuscula (Annonaceae) fueron analizados por CG/EM y comparados. Empleando la fitoquímica usual fueron aislados cinco sesquiterpenos del tipo aromadendrano del aceite esencial de las hojas frescas: allo-aromadendran-14beta-al, (-)-ledol, viridiflorol, (+)-espatulenol y el ácido allo-aromadendran-14beta-oico. El ácido allo-aromadendran-14beta-oico fue también obtenido, habiendo sido generado por la oxidación espontánea de su aldehído correspondiente. Ambos aceites mostraron actividad enfrente Artemia salina
Stability of Citrus tristeza virus protective isolates in field conditions
Costa, Alessandra Tenório;Nunes, William Mário de Carvalho;Zanutto, Carlos Alexandre;Müller, Gerd Walter;
Pesquisa Agropecuária Brasileira , 2010, DOI: 10.1590/S0100-204X2010000700009
Abstract: the objective of this work was to monitor the maintenance of citrus tristeza virus (ctv) protective isolates stability in selected clones of 'pêra' sweet orange (citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. the work was carried out in field conditions in the north of paraná state, brazil. coat protein gene (cpg) analysis of 33 isolates collected from 16 clones of 'pêra' sweet orange was performed using single strand conformational polymorphism (sscp). initially, the isolates were characterized by symptoms of stem pitting observed in clones. then viral genome was extracted and used as template for the amplification of cpg by reverse transcription polimerase chain reaction (rtpcr). rtpcr products electrophoretic profiles were analyzed using the jaccard coefficient and the upgma method. the majority of the clones had weak to moderate stem pitting symptoms and its ctv isolates showed alterations in the sscp profiles. however, the stability of the protective complex has been maintained, except for isolates from two analised clones. low genetic variability was observed within the isolates during the studied years.
Análise clínica e biomecanica do efeito do diclofenaco sódico na consolida??o da fratura da tíbia no rato
Müller, Sérgio Swain;Curcelli, Emílio Carlos;Sardenberg, Trajano;Zuccon, Alexandre;De Crudis Júnior, José Luiz;Padovani, Carlos Roberto;
Acta Ortopédica Brasileira , 2004, DOI: 10.1590/S1413-78522004000400001
Abstract: the antinflammatories are agents utilized on clinical practice that interfere on inflammatory process by synthesis inhibition of prostaglandin and tromboxanes. some experimental studies investigated their action on the fractures consolidation process, through clinical and histological studies, as the biomechanical analyses are scarce. in this study, 20 (twenty) wistar pedigree rats were used, aleatory divided into two groups: a group (control) and b group (treated with diclofenac). in both groups open fractures were made through perforation on right tibia. the drug administration was done intramuscularly in a single daily dose, during 28 (twenty-eight) days. the animals were weekly weighed. after the sacrifice the tibias were dissected, weighed and submitted to a biomechanical analysis of flexion and the maximum load, deformation and rigidity coefficient were measured. it was remarkable that the treated group with antinflammatory did not present body weight gain after the second week and the broken tibias were heavier. in this group the osseous callous supported less maximum load, presenting larger deformation and smaller rigidity coefficient. in the treated group, the non-fractured bone was also less rigid. concluding, at this studied conditions, the diclofenac can interfere on the consolidation process and on the osseous metabolism, promoting a delay on the callous maturation and a smaller rigidity of the intact bone, respectively.
Journal of the Chilean Chemical Society , 2003, DOI: 10.4067/S0717-97072003000400014
Abstract: the essential oils obtained from the dried and fresh leaves of duguetia glabriuscula - (annonaceae) were analyzed by gc/ms and compared. by employing the usual phytochemical workup, five aromadendrane sesquiterpenes were isolated from the essential oil obtained from fresh leaves: alloaromadendran-14b-al, alloaromadendrene, (-)-ledol, viridiflorol, and (+)-spathulenol. alloaromadendran-14b-oic acid was also obtained, having been generated by the spontaneous oxidation of its corresponding aldehyde. both essential oils displayed activity against artemia salina
Comparison of Citrus tristeza virus (CTV) isolates by RFLP analysis of the coat protein nucleotide sequences and by the severity of the symptoms
Corazza, Maria Júlia;Zanutto, Carlos Alexandre;Zanineli-Ré, Maria Lúcia;Müller, Gerd Walter;Nunes, William Mário de Carvalho;
Tropical Plant Pathology , 2012, DOI: 10.1590/S1982-56762012000300003
Abstract: plants of pera sweet orange on rangpur lime rootstocks, from orchards of the northwest and north of paraná state, brazil, were evaluated for severity of symptoms and genetic diversity of citrus tristeza virus. the severity of symptoms was evaluated by the development of tree, fruit size and stem pitting symptoms. isolates that infect these plants were compared with known mild and severe isolates by analysis of restriction fragments length polymorphism (rflp) of the coat protein nucleotide sequences (cpns), amplified by the polymerase chain reaction (pcr) and undergone digestion with the restriction enzymes hinf i and rsa i. the severity of symptoms showed that the analyzed plants from the northwest orchards presented mild to moderate tristeza symptoms, while the plants from the north orchards exhibited moderate to severe symptoms. the rflp analysis revealed that the ctv isolates are constituted by haplotype mixtures. rsa i was the enzyme that best discriminated the genetic diversity among the analyzed isolates of ctv. two main groups were generated by the upgma analysis. the isolates from the northwest orchards grouped with most of the mild isolates used as control, and a great part of the isolates from the north orchards, was correlated with the severe isolate cap?o bonito. correlation between the stem pitting intensity and rflp patterns, was demonstrated with some exceptions. the failure of protection of some isolates and the contamination of the rootstocks by the severe isolates, in field nursery conditions, before grafting with scions with mild isolates, were the hypotheses considered to explain the occurrence of severe ctv isolates in the north area of paraná state.
Caracteriza??o biológica e molecular de isolados do Citrus tristeza virus com potencial para utiliza??o em programas de pré-imuniza??o
Costa, Alessandra Tenório;Nunes, William Mário de Carvalho;Corazza, Maria Júlia;Zanutto, Carlos Alexandre;Müller, Gerd Walter;
Summa Phytopathologica , 2010, DOI: 10.1590/S0100-54052010000100014
Abstract: a cross-protection program citrus tristeza disease, caused by citrus tristeza virus (ctv) has been developed in the parana state, to obtain pera orange mother plants. thus, 20 plants were selected in the orange commercial fields of the north and northwest region. comparisons between mild and severe recognized isolates were made. the results showed mild-to-moderate stem pitting symptoms and the great majority of the isolates grouped up with the mild control isolate. these results suggest that the plants selected were infected by ctv mild haplotypes showing that they have mother plant potential.
Faunistic survey of sandstone caves from Altinópolis region, S?o Paulo State, Brazil
Zeppelini Filho, Douglas;Ribeiro, Alexandre Cunha;Ribeiro, Guilherme Cunha;Fracasso, Maria Paula Aguiar;Pavani, Marcelo Monetti;Oliveira, Otto Müller Patr?o;Oliveira, Sérgio Adriano de;Marques, Antonio Carlos;
Papéis Avulsos de Zoologia (S?o Paulo) , 2003, DOI: 10.1590/S0031-10492003000500001
Abstract: the fauna of eight sandstone caves of the region of altinópolis, (serra geral arenitic speleological province, s?o paulo state, southeastern brazil) was surveyed. our results improve the previous faunistic knowledge of the region, recording 15 new occurrences for brazilian caves and 26 for brazilian sandstone caves. the fauna is characterized by a large number of detritivores/omnivores such as crickets and cockroaches, and several predators like spiders and heteropterans in bat guano.
Bromeliad-associated mosquitoes from Atlantic forest in Santa Catarina Island, southern Brazil (Diptera, Culicidae), with new records for the State of Santa Catarina
Müller, Gerson Azulim;Marcondes, Carlos Brisola;
Iheringia. Série Zoologia , 2006, DOI: 10.1590/S0073-47212006000300007
Abstract: bromeliad-associated mosquitoes (diptera: culicidae) in atlantic forest in florianópolis, santa catarina, southern brazil, were studied, examining plants of vriesea philippocoburgi wawra and aechmea lindenii (e. morren) baker var. lindenii at secondary atlantic rain forest, and a. lindenii and vriesea friburgensis mez var. paludosa (l. b. smith) at "restinga" per month, during 12 months. no immature forms of mosquitoes were collected from a. lindenii in the secondary forest. collections obtained 368 immature mosquitoes, none of them from a. lindenii from rain forest. culex (microculex) spp. constituted 79.8% of the total, wyeomyia (phoniomyia) spp. 17.93%, and anopheles (kerteszia) cruzii (dyar & knab, 1908) only 1.36%. the study shows the great predominance of species of medical importance not yet proved, and the small number of immature stages of anopheline mosquitoes. the rainfall, but not the mean temperatures, significantly influenced the quantity of mosquitoes from v. philippocoburgi. significant differences between the quantities of immature forms of all the bromeliad species were found, and the shape of the plants could be important to the abundance of mosquitoes. all six species of cx. (microculex) found are recorded for the first time in the state of santa catarina, and all six species of wyeomyia (phoniomyia) are recorded for the first time in bromeliads in this state.
Immature mosquitoes (Diptera: Culicidae) on the bromeliad Nidularium innocentii in ombrophilous dense forest of Santa Catarina Island, Florianópolis, Santa Catarina State, southern Brazil
Gerson Azulim Müller,Carlos Brisola Marcondes
Biotemas , 2007,
Abstract: Immature forms of mosquitoes (Diptera: Culicidae) were collected from bromeliads Nidularium innocentii var. paxianum in dense ombrophilous forest of Santa Catarina Island, state of Santa Catarina. Two hundred and eleven mosquitoes were collected; 37.44% Culex (Microculex) albipes, 19.91% Cx. (Mic.) davisi, 0.95% Anopheles (Kerteszia) cruzii and 0.47% Wyeomyia (Phoniomyia) tripartita. Correlation between the quantities of collected mosquitoes and mean temperatures was significantly positive, but that between the quantities and the rainfall was not. The quantity of anophelines was much smaller than that reported by other workers in the same region. Even considering the small quantity of anophelines collected, the great density of this bromeliad in the area indicates the need for of attention to it as a breeding ground for these mosquitoes.
Protozoa Drive the Dynamics of Culturable Biocontrol Bacterial Communities
Maren Stella Müller, Stefan Scheu, Alexandre Jousset
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0066200
Abstract: Some soil bacteria protect plants against soil-borne diseases by producing toxic secondary metabolites. Such beneficial biocontrol bacteria can be used in agricultural systems as alternative to agrochemicals. The broad spectrum toxins responsible for plant protection also inhibit predation by protozoa and nematodes, the main consumers of bacteria in soil. Therefore, predation pressure may favour biocontrol bacteria and contribute to plant health. We analyzed the effect of Acanthamoeba castellanii on semi-natural soil bacterial communities in a microcosm experiment. We determined the frequency of culturable bacteria carrying genes responsible for the production of the antifungal compounds 2,4-diacetylphloroglucinol (DAPG), pyrrolnitrin (PRN) and hydrogen cyanide (HCN) in presence and absence of A. castellanii. We then measured if amoebae affected soil suppressiveness in a bioassay with sugar beet seedlings confronted to the fungal pathogen Rhizoctonia solani. Amoebae increased the frequency of both DAPG and HCN positive bacteria in later plant growth phases (2 and 3 weeks), as well as the average number of biocontrol genes per bacterium. The abundance of DAPG positive bacteria correlated with disease suppression, suggesting that their promotion by amoebae may enhance soil health. However, the net effect of amoebae on soil suppressiveness was neutral to slightly negative, possibly because amoebae slow down the establishment of biocontrol bacteria on the recently emerged seedlings used in the assay. The results indicate that microfaunal predators foster biocontrol bacterial communities. Understanding interactions between biocontrol bacteria and their predators may thus help developing environmentally friendly management practices of agricultural systems.
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