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Search Results: 1 - 10 of 629183 matches for " Carlos A. Mandarim-de-Lacerda "
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Stereological tools in biomedical research
Mandarim-de-Lacerda, Carlos A.;
Anais da Academia Brasileira de Ciências , 2003, DOI: 10.1590/S0001-37652003000400006
Abstract: stereological studies are more and more frequent in literature, particularly in the development/evolution, pathology, and neurosciences areas. the stereology challenge is to understand the structural inner threedimensional arrangement based on the analysis of the structure slices only showing two-dimensional information. cavalieri and scherle's methods to estimate volume, and buffon's needle problem, are commented in the stereological context. a group of actions is needed to appropriately quantify morphological structures (unbiased and reproducibly), e.g. sampling, isotropic and uniform randomly sections (delesse's principle), and updated stereological tools (disector, fractionator, nucleator, etc). through the correct stereology use, a quantitative study with little effort could be performed: efficiency in stereology means a minimum slices sample counting (little work), low cost (slices preparation), but good accuracy. in the present text, a short review of the main stereological tools is done as a background basis to non-expert scientists.
Stereological tools in biomedical research
Mandarim-de-Lacerda Carlos A.
Anais da Academia Brasileira de Ciências , 2003,
Abstract: Stereological studies are more and more frequent in literature, particularly in the development/evolution, pathology, and neurosciences areas. The stereology challenge is to understand the structural inner threedimensional arrangement based on the analysis of the structure slices only showing two-dimensional information. Cavalieri and Scherle's methods to estimate volume, and Buffon's needle problem, are commented in the stereological context. A group of actions is needed to appropriately quantify morphological structures (unbiased and reproducibly), e.g. sampling, isotropic and uniform randomly sections (Delesse's principle), and updated stereological tools (disector, fractionator, nucleator, etc). Through the correct stereology use, a quantitative study with little effort could be performed: efficiency in stereology means a minimum slices sample counting (little work), low cost (slices preparation), but good accuracy. In the present text, a short review of the main stereological tools is done as a background basis to non-expert scientists.
Beneficial Effects of Angiotensin II AT1 Blocker on Cardiovascular Adverse Remodeling Due to Nitric Oxide Synthesis Blockade
Fernandes-Santos,Caroline; Mendon?a,Leonardo de Souza; Mandarim-de-Lacerda,Carlos Alberto;
International Journal of Morphology , 2006, DOI: 10.4067/S0717-95022006000400003
Abstract: we studied with morphological tools the effects of different doses of losartan upon the cardiovascular remodeling in nitric oxide deficient rats. at 15 weeks of age, thirty wistar rats were separated in six groups: control (c), l-name (ln), and four groups were ln was given plus losartan at different doses (1, 5, 20 and 40 mg/kg/day). the l-name was given for 9 weeks, the losartan administration starting on the 2nd week of experiment. we studied the heart, thoracic aorta and superior mesenteric artery with light microscopy and stereology. the blood pressure (bp) increased since the first week of l-name administration, the losartan treatment at doses of 20 and 40 mg/kg/day was efficient to reduce bp after the 7th week of treatment. the cardiac adverse remodeling in the ln group was characterized by intense interstitial fibrosis, impairment of the myocardial microvascularization, cardiomyocyte hypertrophy and consequent loss of cardiomyocytes. the aortic wall structure (density per area of smooth muscle cell nuclei and surface density of lamellae), and the superior mesenteric artery media/lumen ratio were also strongly affected by l-name administration. only in the dose equal or higher than 20 mg/kg/day losartan showed beneficial effects treating these alterations. in conclusion, both the heart and the arterial wall of no deficient rats suffer a marked adverse remodeling process that is efficiently treated by a dose-dependent losartan administration. the efficiency of losartan treatment in this model of no synthesis blockade correlates with the hypotensor effect of the drug mainly in the high dose treatment
Beneficial Effects of Angiotensin II AT1 Blocker on Cardiovascular Adverse Remodeling Due to Nitric Oxide Synthesis Blockade Efectos Beneficiosos del Bloqueador AT1 de la Angiotensina II en la Remodelación Cardiovascular Adversa Causada por el Bloqueo de la Síntesis de óxido Nítrico
Caroline Fernandes-Santos,Leonardo de Souza Mendon?a,Carlos Alberto Mandarim-de-Lacerda
International Journal of Morphology , 2006,
Abstract: We studied with morphological tools the effects of different doses of Losartan upon the cardiovascular remodeling in nitric oxide deficient rats. At 15 weeks of age, thirty Wistar rats were separated in six groups: control (C), L-NAME (LN), and four groups were LN was given plus Losartan at different doses (1, 5, 20 and 40 mg/kg/day). The L-NAME was given for 9 weeks, the Losartan administration starting on the 2nd week of experiment. We studied the heart, thoracic aorta and superior mesenteric artery with light microscopy and stereology. The blood pressure (BP) increased since the first week of L-NAME administration, the Losartan treatment at doses of 20 and 40 mg/kg/day was efficient to reduce BP after the 7th week of treatment. The cardiac adverse remodeling in the LN group was characterized by intense interstitial fibrosis, impairment of the myocardial microvascularization, cardiomyocyte hypertrophy and consequent loss of cardiomyocytes. The aortic wall structure (density per area of smooth muscle cell nuclei and surface density of lamellae), and the superior mesenteric artery media/lumen ratio were also strongly affected by L-NAME administration. Only in the dose equal or higher than 20 mg/kg/day Losartan showed beneficial effects treating these alterations. In conclusion, both the heart and the arterial wall of NO deficient rats suffer a marked adverse remodeling process that is efficiently treated by a dose-dependent Losartan administration. The efficiency of Losartan treatment in this model of NO synthesis blockade correlates with the hypotensor effect of the drug mainly in the high dose treatment Se estudiaron con herramientas morfológicas, los efectos de diferentes dosis de Losartan sobre el remodelamiento cardiovascular, en ratas deficientes en óxido nítrico. 30 ratas Wistar, con 15 semanas de edad, fueron separadas en 6 grupos. control (C), L-NAME (LN), y 4 grupos en que administró LN junto con Losartan, en diferentes dosis (1, 5, 20 y 40 mg/kg/dia). El L-NAME fue administrado durante 9 semanas y la administración de Losartan se inició en la segunda semana de experimentación. Se estudiaron el corazón, la parte torácica de la aorta y la arteria mesentérica craneal, con microscopía de luz y estereología. La presión arterial (PA) aumentó desde la primera semana de administración de L-NAME. El tratamiento con Losartan, en las dosis de 20 y 40 mg/kg/día, fue eficiente para reducir la PA después de la séptima semana de tratamiento. El remodelamiento cardiaco adverso en el grupo LN se caracterizó por intensa fibrosis intersticial, disminución de la
Simple hyperplasia versus proliferative endometrium: stereological study
Avvad-Portari, Elyzabeth;Gomes, Nádia D.;Mandarim-de-Lacerda, Carlos A.;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2003, DOI: 10.1590/S1676-24442003000100014
Abstract: the diagnosis of some forms of hyperplastic endometrium continues to present difficulties for the practicing pathologist. one of the major problems is the lack of a standard terminology for endometrial classification. therefore, morphometrical techniques could help the accurate diagnosis. thirteen samples of endometrium with simple hyperplasia and thirteen samples of proliferative endometrium were analyzed by light microscopy and stereology. we determined the volume density, the surface density and the length density (vv, sv, lv) of the glands (epithelium and lumen) and the stroma. volume densities of the epithelium and lumen as well as the surface density of the inner perimeter of the glands were greater in simple hyperplasia than in proliferative endometrium (p < 0.05). but the volume density of the stroma was greater in proliferative endometrium than in simple hyperplasia (p < 0.05). the length density of the glands and the surface density of the outer perimeter were not different comparing these two groups. present results agree with previous studies that have pointed out the importance of quantitative parameters in the diagnosis of the endometrial pathologies offering new stereological parameters to this analysis.
Papel da óxido nítrico sintase na etiopatogenia da estenose hipertrófica do piloro na infancia
Barbosa, Irnak M.;Ferrante, Saulo M.R.;Mandarim-de-Lacerda, Carlos A.;
Jornal de Pediatria , 2001, DOI: 10.1590/S0021-75572001000400013
Abstract: objective: to experimentally reproduce, in rats, the findings corresponding to the histopathology of infantile hypertrophic pyloric stenosis (ihsp), using nitric oxide synthase (nos) inhibitor (l-name). methods: l-name was administered to pregnant rats (l-name group), from the 14th gestational day on in order to reproduce the model of nos inhibition in the production of ihsp. this group was then compared to control animals. after birth, all the animals in the l-name group were maintained under nos inhibition until the 42nd day of life, when they were sacrificed. the control animals, which did not receive any kind of drug, were also sacrificed on the 42nd day of life. the animals and their internal organs were analyzed and weighed. the pyloric region was technically prepared and observed through light microscopy. results: the l-name group presented lower body and intestinal weight and higher gastric weight than the control group. light microscopy revealed hypertrophy of the circular smooth muscle layer of the pyloric muscle in l-name animals. conclusions: this work reproduced an experimental model of an ihsp study, confirming the effect of nos blockade on the pyloric musculature. conclusions: this work reproduced an experimental model of an ihsp study, confirming the effect of nos blockade on the pyloricmusculature.
COMPARISON BETWEEN COMPUTED TOMOGRAPHY AND ULTRASONOGRAPHY IN THE CEREBRAL VENTRICLES MORPHOMETRY
Boasquevisque,Edson M.; Mandarim-de-Lacerda,Carlos A.; Cerri,Giovani G.;
Revista chilena de anatomía , 2000, DOI: 10.4067/S0716-98682000000100002
Abstract: encephalic ventricles were evaluated by ct and us in normal subjects and in patients suffering of meningitis. the biometry and statistical analyses were performed observing the ventricular index (vi%), and the diameters of the frontal and temporal horns of the lateral ventricle, and the diameters of the iii and iv ventricles. comparing the two diagnostic methods the coefficient of correlation was high for the lateral ventricle diameter (r=0.94) and for the vi (0.85). the sensitivity of the us was better than the ct in the observation of the temporal horn diameter. the us, because it high accuracy, it low cost and the possibility to be a portable equipment, is a useful method to investigate cerebral ventricles in infants
COMPARISON BETWEEN COMPUTED TOMOGRAPHY AND ULTRASONOGRAPHY IN THE CEREBRAL VENTRICLES MORPHOMETRY COMPARACIóN ENTRE TOMOGRAFíA COMPUTARIZADA Y ULTRA-SONOGRAFíA EN LA MORFOMETRíA DE LOS VENTRíCULOS CEREBRALES
Edson M. Boasquevisque,Carlos A. Mandarim-de-Lacerda,Giovani G. Cerri
Revista chilena de anatomía , 2000,
Abstract: Encephalic ventricles were evaluated by CT and US in normal subjects and in patients suffering of meningitis. The biometry and statistical analyses were performed observing the ventricular index (VI%), and the diameters of the frontal and temporal horns of the lateral ventricle, and the diameters of the III and IV ventricles. Comparing the two diagnostic methods the coefficient of correlation was high for the lateral ventricle diameter (r=0.94) and for the VI (0.85). The sensitivity of the US was better than the CT in the observation of the temporal horn diameter. The US, because it high accuracy, it low cost and the possibility to be a portable equipment, is a useful method to investigate cerebral ventricles in infants Ventrículos encefálicos fueron evaluados por CT y US en sujetos normales y en pacientes afectados de meningitis. La biometría y el análisis estadístico fueron ejecutados observando el índex ventricular (VI%), los diámetros de los cuernos frontal y temporal del ventrículo lateral, y los diámetros de los ventrículos III y IV. Comparando los dos métodos diagnósticos, el coeficiente de correlación fue alto para el diámetro del ventrículo lateral (r=0.94) y para el VI (0.85). La sensibilidad de la US fue mejor que para la CT en la observación del diámetro del cuerno temporal. La US, por ser de alta precisión, de bajo costo y por emplear un equipamiento transportable, resulta un método útil para la investigación de los ventrículos cerebrales
Simple hyperplasia versus proliferative endometrium: stereological study
Avvad-Portari Elyzabeth,Gomes Nádia D.,Mandarim-de-Lacerda Carlos A.
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2003,
Abstract: The diagnosis of some forms of hyperplastic endometrium continues to present difficulties for the practicing pathologist. One of the major problems is the lack of a standard terminology for endometrial classification. Therefore, morphometrical techniques could help the accurate diagnosis. Thirteen samples of endometrium with simple hyperplasia and thirteen samples of proliferative endometrium were analyzed by light microscopy and stereology. We determined the volume density, the surface density and the length density (Vv, Sv, Lv) of the glands (epithelium and lumen) and the stroma. Volume densities of the epithelium and lumen as well as the surface density of the inner perimeter of the glands were greater in simple hyperplasia than in proliferative endometrium (p < 0.05). But the volume density of the stroma was greater in proliferative endometrium than in simple hyperplasia (p < 0.05). The length density of the glands and the surface density of the outer perimeter were not different comparing these two groups. Present results agree with previous studies that have pointed out the importance of quantitative parameters in the diagnosis of the endometrial pathologies offering new stereological parameters to this analysis.
Advantages of Evaluating Mean Nuclear Volume as an Adjunct Parameter in Prostate Cancer
Eduardo Leze, Clarice F. E. Maciel-Osorio, Carlos A. Mandarim-de-Lacerda
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0102156
Abstract: Background Efforts to improve the diagnosis, prognosis and surveillance of prostate cancer (PCa) are relevant. Gleason score (GSc) overestimation may subject individuals to unnecessary aggressive treatment. We aimed to use stereology in PCa evaluations and investigate whether mean nuclear volume (MNV) correlates with the Gleason primary pattern (Gpp) and to improve the subjective GSc to obtain an objective and reliable method without inter-observer dissension. Methods We identified 74 radical prostatectomy specimens that were divided into six groups based on Gpp, from 3 to 5. Controls (C) were designed in paired non-tumor regions of the same specimens. MNV was estimated using the “point-sampled intercepts” method. Differences in MNV among the C groups and the Gpp groups were tested with the Kruskall-Wallis test and Dunn post-hoc test. Differences between each Gpp group and its control counterpart were tested with the Wilcoxon test. Correlations were evaluated with the Spearman rank correlation (R[Spearman]). Results The correlations between prostate-specific antigen (PSA) and GSc (R[Spearman] of 0.76) and between PSA and MNV (R[Spearman] of 0.78) were moderately strong and highly significant, and the correlation between MNV and Gpp (R[Spearman] of 0.53) was moderate and highly significant. MNV was significantly greater in cancerous regions than in paired-control regions. Limitations included sample size. Conclusions Proper planning of a study, as well as the availability of equipment and software for morphological quantification, can provide incentive to quickly and accurately estimate MNV as an adjunct parameter in the assessment of PCa. Current data are in favor of the use of MNV associated with GSc and PSA in the assessment of PCa.
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