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Search Results: 1 - 10 of 79978 matches for " Carla Luiza da Silva; "
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Aerobic stability of sugar cane silages with a novel strain of Lactobacillus sp. isolated from sugar cane
ávila, Carla Luiza da Silva;Pinto, José Cardoso;Oliveira, Damiany Pádua;Schwan, Rosane Freitas;
Revista Brasileira de Zootecnia , 2012, DOI: 10.1590/S1516-35982012000200003
Abstract: the objective of this research was to evaluate the effect of the addition of two lactobacillus strains on the aerobic stability of the sugar cane (saccharum spp.) silages. the experiment was carried out in a completely randomized design with three replicates in split-plot in time scheme, so that the treatments (silages without inoculant and with novel strain or commercial inoculants) were randomized on plot and in times of aerobic evaluation of the silages (zero, two, four, and six days after the opening of the silos) as subplots. the loss of stability of silages was continuous during the time of aerobic exposure. inoculants influenced the changes in populations of lactic acid bacteria and yeasts and in the ammoniacal nitrogen, acetic acid and ethanol concentrations. the dry matter, crude protein, soluble carbohydrates contents and ph values showed similar changes in all three silages studied. a linear increase in crude protein content and ph values and decrease in the soluble carbohydrates content were observed. the dry matter contents increased to a maximum of 335 g/kg on fresh matter at four days, followed by reduction after six days. the results showed that the use of inoculants is recommended because it promoted higher production of acetic and propionic acids, reducing the population of yeast and, therefore, improving the aerobic stability of silages.
Avalia??o dos conteúdos de carboidratos solúveis do capim-tanzania ensilado com aditivos
ávila, Carla Luiza da Silva;Pinto, José Cardoso;Tavares, Valdir Botega;Santos, ívina Paula Almeida dos;
Revista Brasileira de Zootecnia , 2006, DOI: 10.1590/S1516-35982006000300004
Abstract: the aim of this study was to check on the effect of additive addition on the variations in the water soluble carbohydrate contents of the tanzaniagrass silage during the fermentation period, and to know the buffering capacity values and water soluble carbohydrate: buffering capacity ratio of this forage. tanzaniagrass (panicum maximum jacq. cv. tanzania i) was established in a clavey red dusk latosol and harvested at 60-65 day of age. the forage was ensiled in experimental pvc silos, fitted with type bunsen valves, with a capacity of about 3 kg each. the treatments consisted of four doses (3, 6, 9 and 12%) of three additives (citrus pulp, wheat meal and corn meal), plus one check without additives and 8 times of silo opening (0, 1, 2, 3, 7, 14, 28 and 56 days) building up a factorial scheme with an additional treatment [(3 x 4) + 1] x 8 with 3 replicates. the experiment was run in a completely randomized block design. water soluble carbohydrate content of tanzaniagrass at 60-65 days of age was low and all of it was consumed during the fermentation process, regardless of additive treatments. all three additives, mainly the citrus pulp, favored the water-soluble carbohydrate contents and buffering capacity of the silages. an increase of the water soluble-carbohydrate: buffering capacity ratio contributes to the yield of better quality silage by assisting in the fermentation process.
Utiliza o do composto exaurido de Pleurotus sajor caju em ra es de frangos de corte e seus efeitos no desempenho dessas aves = Utilization of the spent substrate of Pleurotus sajor caju mushroom in broiler chicks ration and the effect on broiler chicken performance
Raquel Santos Azevedo,Carla Luiza da Silva ávila,Eustáquio Souza Dias,Ant?nio Gilberto Bertechini
Acta Scientiarum : Animal Sciences , 2009,
Abstract: Avaliou-se a adi o dietética de um composto exaurido da produ o docogumelo Pleurotus sajor caju sobre o desempenho de frangos de corte nos períodos de um a 21, 22 a 38 e um a 38 dias de idade. Foram utilizados 500 pintos de um dia Ross-308, machos, em delineamento inteiramente casualizado, com cinco tratamentos, obtidos pelosníveis do composto na ra o (0; 0,5; 1,0; 1,5 e 2,0%) com quatro repeti es de 20 aves cada. Foram avaliados ganho de peso, consumo de ra o, convers o alimentar, rendimento de carca a, gordura abdominal e altura das microvilosidades do intestino. A adi o do composto n o influenciou no consumo da ra o e na convers o alimentar. Para o ganho de peso houve efeito positivo somente na fase inicial (um a 21 dias), sendo o valor máximo obtido com a adi o de 0,67% do composto. A adi o do composto n o alterou o rendimento de carca a e gordura abdominal, porém, alterou a altura das microvilosidades do intestino. A adi o de composto exaurido da produ o do fungo Pleurotus sajor caju, na concentra o de 0,67%, melhora o ganho de peso dos frangos nos primeiros 21 dias de idade. This research evaluated the effect of the addition of a spent mushroom substrate (SMS) Pleurotus sajor caju at different levels on the performance of broiler chicks from 1 to 21, 22 to 38 and 1 to 38days of age. Five hundred one-day-old Ross-308 chicks were utilized, allocated in a completely randomized design, with five treatments obtained by increased levels of compost on ration (0; 0.5; 1.0; 1.5 and 2.0%), with four replicates of 20 birds per experimental unit. The intake, weight gain, feed conversion, carcass yield, abdominal fatand villus height were evaluated. No effect was observed on intake and feed conversion when the compost was included in the feeding. A positive effect was observed for weight gain from 1 to 21 days of age, with maximum value of 0.67% of SMS, but its addition didnot modify the carcass yield and abdominal fat, but modified the villus height. The inclusion of the spent substrate of Pleurotus sajor caju mushroom up to 0.67% improves the weight gain of broiler chicks in the first 21 days of age.
Acessibilidade geográfica à aten??o primária à saúde em distrito sanitário do município de Salvador, Bahia
Silva Júnior, Evanildo Souza da;Medina, Maria Guadalupe;Aquino, Rosana;Fonseca, Ana Carla Freitas;Vilasb?as, Ana Luiza Queiroz;
Revista Brasileira de Saúde Materno Infantil , 2010, DOI: 10.1590/S1519-38292010000500005
Abstract: objectives: to assess the geographical accessibility of primary health care for the population served by the community health agents program (pacs) and the family health program (psf) in one sanitary district in the city of salvador, brazil. methods: a evaluative cross-sectional study was carried out. the data were collected using interviews with health workers from the eleven pacs and psf teams. analysis of the data took two factors into account: access of the population and the logic of territorialization. results: the district has a relatively rugged landscape and all the teams reported the existence of geographical barriers in the area, in particular steps and steep hills. there was no difference in accessibility between the pacs and the psf. the process of territorialization was unrelated to the concept of territory as a process. some areas covered by the teams were located some distance from the ubs and this constituted a significant obstacle to access. conclusions: the implantation of the pacs/psf strategy in the district and the model of territorialization were not in line with the principles that should guide primary health care, resulting in geographical restrictions on access for the population served. it is recommended that the current territorial design be reviewed to ensure that it is more line with psf guidelines and to enable better access to primary care.
Efeitos da compacta??o, da inclus?o de aditivo absorvente e do emurchecimento na composi??o bromatológica de silagens de capim-tanzania
Tavares, Valdir Botega;Pinto, José Cardoso;Evangelista, Ant?nio Ricardo;Figueiredo, Henrique César Pereira;ávila, Carla Luiza da Silva;
Revista Brasileira de Zootecnia , 2009, DOI: 10.1590/S1516-35982009000100006
Abstract: the objective of this work was to evaluate the effects of different compaction degrees, inclusion of absorbent additive and wilting on the chemical composition of tanzania grass silages. the experiment was conducted using tanzania grass (panicum maximum jacq. cv. tanzania i) harvested at 60-65 days of growth. the forage was ensiled in experimental pvc silos fitted with bunsen type valves, with capacity for about 4 and 8 kg each. the treatments consisted of three types of silages (control, 5% of citric pulp and pre-wilted) with five compaction degrees (400, 500, 600, 700 and 900 kg/m3). the experimental design was random blocks, with four replicates. citric pulp and pre-wilting increased the dry matter content of tanzania grass silages. the increased density resulted into decreases in the ph values and amoniacal-n contents; the greatest densities resulted in ph values within the ideal range. the presence of citric pulp and the pre-wilting practice reduce the losses by effluent and gas relative to the control. as the density increased, the amount of effluent increased in the control silage and decreased in silages with citric pulp.
Microdissec??o e captura a laser na investiga??o do gene TP53 em tecidos incluídos em parafina
Ihlaseh, Shadia Muhammad;Oliveira, Maria Luiza Cotrim Sartor de;Silva, Glenda Nicioli da;Franchi, Carla Adriene da Silva;Camargo, Jo?o Lauro Viana de;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2007, DOI: 10.1590/S1676-24442007000100012
Abstract: backgorund: laser-capture micro-dissection (lcm) is a recently developed procedure that provides single cells or specific cell groups for molecular analysis. currently, there are few lcm systems in brazil, in such a way that it is necessary to disseminate the technical procedures inherent to the methodology, and also to characterize its enormous potential for diagnosis and research. objective: this study describes the standardization of lcm and dna extraction from formalin fixed and paraffin-embedded tissues. material and method: the gene tp53 exon 8 and the cyclophilin gene were studied in normal and neoplastic liver and kidney samples from a chemical carcinogenesis model in rat. dna extraction was confirmed by nested-pcr. results: histological sections preparation for lcm and the nested-pcr procedures were standardized; 48.3% amplifications of the gene tp53 exon 8 and 51.7% of the cyclophilin gene samples were obtained. when at least one of the gene segments was considered, 79.3% samples presented amplification. discussion and conclusion: procedures for dna extraction from formalin fixed and paraffin-embedded tissues collected by lcm were standardized. they can be useful for dna collection for molecular studies.
Perfil de fermenta??o das silagens de capim-tanzania com aditivos teores de nitrogênio amoniacal e ph
ávila, Carla Luiza da Silva;Pinto, José Cardoso;Evangelista, Ant?nio Ricardo;Morais, Augusto Ramalho de;Figueiredo, Henrique Cesar Pereira;Tavares, Valdir Botega;
Ciência e Agrotecnologia , 2003, DOI: 10.1590/S1413-70542003000500024
Abstract: in general, forage grasses harvested at younger development stage present low contents of dm that associated to the low content of soluble carbohydrates, can harm the fermentation harming the final quality of the silage. thus, it is indispensable to adopt procedures which modify this picture such as the use of additives. this work aimed to evaluate the fermentation of tanzania grass silages with additives in relation to the ammonia-n content and ph, and among these additives, to determine which of them and what dose confers the best qualities to the produced silage. the experiment was carried out in the animal science department of ufla by utilizing tanzaniagrass (panicum maxium jacq. cv. tanzania i) established in a clayey dusky red latosol and harvested at 60-65 days of growth. the forage was ensiled in experimental pvc silos, adapted with valves type bunsen with capacity of about 3 kg each. the treatments consisted of three additives (citrus pulp, wheat meal and corn meal) in four doses (3, 6, 9 and 12%) plus one without additives and 8 times of silo opening (0, 1, 2, 3, 7, 14, 28 and 56 days). the experimental design utilized was randomized blocks, constituing a factorial scheme with additional treatment [(3x4) + 1] x 8 with 3 replicates. the addition of citrus pulp, wheat meal and corn meal improves the fermentative characteristics of the tanzaniagrass silage. the silages without additives present values of ph and ammonia-n characteristics of a satisfactory quality silage but the use of additives is recommended to prevent losses.
Estabilidade aeróbia de silagens de capim-momba?a tratadas com Lactobacillus buchneri
ávila, Carla Luiza da Silva;Pinto, José Cardoso;Figueiredo, Henrique César Pereira;Morais, Augusto Ramalho de;Pereira, Odilon Gomes;Schwan, Rosane Freitas;
Revista Brasileira de Zootecnia , 2009, DOI: 10.1590/S1516-35982009000500001
Abstract: this study aimed to evaluate the effect of the addition of two l. buchneri strains on the aerobic stability of the momba?a grass silages. the experiment was carried out in a completely randomized design with three replicates in split-plot in time scheme, so that the treatments (silages without inoculant and with experimental or commercial inoculants) were randomized on plot and in times of aerobic evaluation of the silages (0, 2, 8, and 10 days after the opening of the silos) as subplots. after an storage period of 90 days, the silos were opened and samples of about 2,5 kg were collected for the evaluation of the aerobic stability based on the temperature for each sample. the chemical and microbiological changes in the silages were evaluated. as soon as the silos were opened, changes in the silages were observed, with increases in the lactic acid bacteria, yeast and filamentous fungus populations. also, the ph values increased due to the reduction in the organic acids concentration and to the increase in the dm and cp contents. the addition of l.buchneri to the silages increased their stability. the silages without inoculant was stable for 55 hours. the inoculated silages remained stable for 10 days. the improved stability was a consequence of an association of high acetic acid concentration with propionic acid and lower lactic acid concentration at the moment of silo opening, which resulted in inhibition of yeasts and molds.
Chemical and microbiological characteristics of sugar cane silages treated with microbial inoculants
ávila, Carla Luiza da Silva;Valeriano, Alexandre Rocha;Pinto, José Cardoso;Figueiredo, Henrique César Pereira;Rezende, Adauton Vilela de;Schwan, Rosane Freitas;
Revista Brasileira de Zootecnia , 2010, DOI: 10.1590/S1516-35982010000100004
Abstract: the aim of this research was to evaluate the effect of microbial additives containing heterofermentative or homofermentative bacteria on the chemical and microbiological characteristics of sugar cane (saccharum spp) silages. sugar cane was inoculated with lactobacillus plantarum, l. paracasei, l. brevis or l. buchneri previously isolated from sugar cane silages or commercial inoculants containing l. buchneri or l. plantarum. silages were produced in laboratory silos (10 × 60 cm pvc tubes) and evaluated 90 days after ensiling. a randomized complete design was used with eight treatments (seven inoculants and control - without inoculant) and three replications. the inoculation with bacteria affected lactic acid bacteria, yeast populations, volatile fatty acids and ethanol contents in the silages. inoculation with different strains of the same species of bacteria result in silages with different chemical and microbiological characteristics. two of the l. buchneri strains show the best results in relation to silage quality.
Efeito da adi??o de Lactobacillus sp. na ensilagem de cana-de-a?úcar
Valeriano, Alexandre Rocha;Pinto, José Cardoso;ávila, Carla Luiza da Silva;Evangelista, Ant?nio Ricardo;Tavares, Valdir Botega;Schwan, Rosane Freitas;
Revista Brasileira de Zootecnia , 2009, DOI: 10.1590/S1516-35982009000600006
Abstract: this study was aimed to evaluate the effect of microbial additives containing hetero fermentative or homo fermentative bacteria on the characteristics of sugarcane silages. sugarcane was inoculated with the following bacteria: lactobacillus plantarum (lp), l. paracasei (lpara), l. brevis (lbrevis), l.buchneri (lb), isolated from sugarcane silage, and three commercial inoculants, two of them containing l. buchneri (pioneer 11a44tm-lbp and lalsil cana-lblc) and one l. plantarum (biomax?-lpb) and control (without inoculation). all the inoculants were applied in population of 105 log cfu.g-1 of fresh forage. a completely randomized design was used with eight treatments and three replicates. the effect of inoculation on the bromatological characteristics was variable. the silage containing l. plantarum (lpb) showed significantly higher dry matter content (32.6%), while all the others strains studied showed similar effect, with average of 28.4%. the crude protein content ranged from 3.6-4.9% and ndf content ranged from 60-66.7% dm. the adf, hemicellulose and ash contents were similar for all silages. all treatments used more than 85% of the soluble carbohydrate, which resulted in ph around 3.5. the ph and ammoniacal nitrogen values were acceptable. the inoculants were more effective for the aerobic evaluation of silages, because they slow down temperature and improve the aerobic stability with best results observed in treatments lblc, lb and lpb.
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