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Search Results: 1 - 10 of 807 matches for " CM;Dei-Cas "
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Pneumocystis diversity as a phylogeographic tool
Derouiche, S;Deville, M;Taylor, ML;Akbar, H;Guillot, J;Carreto-Binaghi, LE;Pottier, M;Aliouat, EM;Aliouat-Denis, CM;Dei-Cas, E;Demanche, C;
Memórias do Instituto Oswaldo Cruz , 2009, DOI: 10.1590/S0074-02762009000100017
Abstract: parasites are increasingly used to complement the evolutionary and ecological adaptation history of their hosts. pneumocystis pathogenic fungi, which are transmitted from host-to-host via an airborne route, have been shown to constitute genuine host markers of evolution. these parasites can also provide valuable information about their host ecology. here, we suggest that parasites can be used as phylogeographic markers to understand the geographical distribution of intra-specific host genetic variants. to test our hypothesis, we characterised pneumocystis isolates from wild bats living in different areas. bats comprise a wide variety of species; some of them are able to migrate. thus, bat chorology and migration behaviour can be approached using pneumocystis as phylogeographic markers. in the present work, we find that the genetic polymorphisms of bat-derived pneumocystis are structured by host chorology. therefore, pneumocystis intra-specific genetic diversity may constitute a useful and relevant phylogeographic tool.
Dermatitis atópica en ni?os: estudio comparativo en dos grupos etarios
Dei-Cas,Pablo G; Acu?a,María Karina; Dei-Cas,Ignacio;
Revista chilena de pediatría , 2011, DOI: 10.4067/S0370-41062011000500006
Abstract: introduction: prevalence of atopic dermatitis (ad) has increased steadily. risk factors are being researched worldwide. there is little data available in agentina. objetives y methods: this study included 722 children > 12 and < 60 months of age residing in an urban community in buenos aires. uk's working party's criteria were used to establish the prevalence of ad. the objectives were a) to establish the prevalence of in children < 36 and > 36 months of age, b) evaluate gender differences in prevalence, and c) evaluate association between ad and the following variables: family history of atopia and degree of consanguinity; number of people living together < 4; use of synthetic clothing in direct contact with the skin; tobacco use within the home; use of rugs/carpeting within the home; environmental contaminating factors in each age group. χ2 was used to calcúlate associations in a logistic regression model. results: prevalence of ad was 31,3% (ci: 27,235,6) among children < 36 months and 49,6% (ci: 43,1-56,1) among children > 36 months. ad was more prevalent among younger males. family history of atopia, use of synthetic material, and living near a factory were associated to ad in both age groups. using rugs/carpeting inside the house was associated to the disease only in younger children. conclusion: ain elevated prevalence of ald was found in this low-income population. it predominates among older children, which differs from commonly accepted findings. not all evaluated risk factors were associated to the disease.
Dermatitis atópica en ni os: estudio comparativo en dos grupos etarios Atopic dermatitis in children: a comparative survey among 2 age groups
Pablo G Dei-Cas,María Karina Acu?a,Ignacio Dei-Cas
Revista chilena de pediatría , 2011,
Abstract: Introducción: La prevalencia de la dermatitis atópica (DA) ha aumentado sostenidamente y sus factores de riesgo son investigados mundialmente. En la Agentina, sin embargo, existen escasos datos disponibles. Objetivos y Métodos: Estudio de prevalencia, de corte transversal que incluyó 722 ni os > 12 y < 60 meses de edad pertenecientes a una comunidad urbana bonaerense. Se utilizaron los criterios del the U.K. Working Party's para definir DA. Los objetivos fueron establecer la prevalencia de la DA en ni os < 36 meses y > 36 meses de edad, conocer si existen diferencias de prevalencia según sexo, y evaluar asociaciones entre DA y las siguientes variables: presencia de antecedentes familiares de atopía según grado, número de convivientes < 4, uso de ropa sintética en contacto directo con la piel, consumo de tabaco en el interior de la vivienda, alfombra/s dentro del hogar y factores contaminantes medioambientales en cada grupo etario. Se utilizó la prueba de χ2 para calcular asociaciones, que de ser significativas se incluyeron en un modelo de regresión logística. Resultados: La prevalencia de DA fue del 31,3% (IC: 27,2-35,6) en los < 36 meses y 49,6% (IC: 43,1-56,1) en los > 36 meses. La DA prevaleció en el sexo masculino en los ni os más peque os. El AAF de atopía, el uso de ropa de material sintético y habitar cerca de una fábrica se relacionaron con la DA en ambos grupos etarios, en tanto poseer alfombra/s dentro del hogar se asoció con la enfermedad sólo en los ni os peque os. Conclusión: Hallamos una elevada prevalencia de DA en esta población de bajos recursos, y que la DA predominó en ni os mayores, resultados que se contraponen a lo actualmente aceptado, y que no todos los factores de riesgo investigados se asociaron con la enfermedad. Introduction: Prevalence of atopic dermatitis (AD) has increased steadily. Risk factors are being researched worldwide. There is little data available in Agentina. Objetives y Methods: This study included 722 children > 12 and < 60 months of age residing in an urban community in Buenos Aires. UK's Working Party's criteria were used to establish the prevalence of AD. The objectives were a) to establish the prevalence of in children < 36 and > 36 months of age, b) evaluate gender differences in prevalence, and c) evaluate association between AD and the following variables: family history of atopia and degree of consanguinity; number of people living together < 4; use of synthetic clothing in direct contact with the skin; tobacco use within the home; use of rugs/carpeting within the home; environmental contaminating fac
Néstor Ponce,Norah Giraldi?Dei-Cas
Amerika : Mémoires, Identités, Territoires , 2011,
Abstract: Ce numéro 5 d’Amerika aborde une thématique qui a suscité un vif intérêt parmi les chercheurs travaillant sur les Amériques et sur les rapports Europe / Amériques. Dans la continuité de notre politique de collaboration avec d’autres groupes de recherche (France, Belgique, Espagne, écosse, Mexique, Argentine, Brésil, Uruguay), nous avons travaillé pour la réalisation de ce dossier avec l’équipe de chercheurs du réseau international NEOS/NEWS (Nords, Suds, Ests, Ouests) Les Amériques et plus pa...
Pitiríase rósea purpúrica: relato de caso e revis o da literatura
Carbia Sergio Gabriel,Chain Myriam,Dei-Cas Ignacio,Hochman Adrián
Anais Brasileiros de Dermatologia , 2003,
Abstract: A pitiríase rósea purpúrica constitui doen a rara e 10 casos foram publicados na Europa e EUA. O quadro clínico cutaneo é a forma hemorrágica ou purpúrica com variável descama o marginal. Relata-se o caso de um homem de 25 anos de idade com les es na regi o escapular. A revis o da literatura enfatiza o diagnóstico diferencial das les es purpúricas. Segundo Lilacs e Medline, n o foram relatados casos na literatura latino-americana.
Bisbenzamidines as Antifungal Agents. Are Both Amidine Functions Required to Observe an Anti-Pneumocystis carinii Activity?
Julien Laurent,Dimitri Stanicki,Tien L. Huang,Eduardo Dei-Cas,Muriel Pottier,El Mouktar Aliouat,Jean Jacques Vanden Eynde
Molecules , 2010, DOI: 10.3390/molecules15064283
Abstract: A library of 19 novel 4-(4-phenylpiperazine-1-yl)benzamidines has been synthesized and evaluated in vitro against Pneumocystis carinii. Among these compounds, N-ethyl- and N-hexyl-4-(4-phenylpiperazine-1-yl)benzamidines emerged as the most promising compounds, with inhibition percentages at 10.0 μg/mL of 87% and 96%, respectively. Those compounds remained active at 0.1 μg/mL.
The Pneumocystis life cycle
Aliouat-Denis, Cécile-Marie;Martinez, Anna;Aliouat, El Moukhtar;Pottier, Muriel;Gantois, Nausicaa;Dei-Cas, Eduardo;
Memórias do Instituto Oswaldo Cruz , 2009, DOI: 10.1590/S0074-02762009000300004
Abstract: first recognised as "schizonts" of trypanosoma cruzi, pneumocystis organisms are now considered as part of an early-diverging lineage of ascomycetes. as no robust long-term culture model is available, most data on the pneumocystis cell cycle have stemmed from ultrastructural images of infected mammalian lungs. although most fungi developing in animals do not complete a sexual cycle in vivo, pneumocystis species constitute one of a few exceptions. recently, the molecular identification of several key players in the fungal mating pathway has provided further evidence for the existence of conjugation and meiosis in pneumocystisorganisms. dynamic follow-up of stage-to-stage transition as well as studies of stage-specific proteins and/or genes would provide a better understanding of the still hypothetical pneumocystislife cycle. although difficult to achieve, stage purification seems a reasonable way forward in the absence of efficient culture systems. this mini-review provides a comprehensive overview of the historical milestones leading to the current knowledge available on the pneumocystis life cycle.
Ploidy of Cell-Sorted Trophic and Cystic Forms of Pneumocystis carinii
Anna Martinez,El Moukhtar Aliouat,Annie Standaert-Vitse,Elisabeth Werkmeister,Muriel Pottier,Claire Pin?on,Eduardo Dei-Cas,Cécile-Marie Aliouat-Denis
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0020935
Abstract: Once regarded as an AIDS-defining illness, Pneumocystis pneumonia (PcP) is nowadays prevailing in immunocompromised HIV-negative individuals such as patients receiving immunosuppressive therapies or affected by primary immunodeficiency. Moreover, Pneumocystis clinical spectrum is broadening to non-severely-immunocompromised subjects who could be colonized by the fungus while remaining asymptomatic for PcP, thus being able to transmit the infection by airborne route to susceptible hosts. Although the taxonomical position of the Pneumocystis genus has been clarified, several aspects of its life cycle remain elusive such as its mode of proliferation within the alveolus or its ploidy level. As no long-term culture model exists to grow Pneumocystis organisms in vitro, an option was to use a model of immunosuppressed rat infected with Pneumocystis carinii and sort life cycle stage fractions using a high-through-put cytometer. Subsequently, ploidy levels of the P. carinii trophic and cystic form fractions were measured by flow cytometry. In the cystic form, eight contents of DNA were measured thus strengthening the fact that each mature cyst contains eight haploid spores. Following release, each spore evolves into a trophic form. The majority of the trophic form fraction was haploid in our study. Some less abundant trophic forms displayed two contents of DNA indicating that they could undergo (i) mating/fusion leading to a diploid status or (ii) asexual mitotic division or (iii) both. Even less abundant trophic forms with four contents of DNA were suggestive of mitotic divisions occurring following mating in diploid trophic forms. Of interest, was the presence of trophic forms with three contents of DNA, an unusual finding that could be related to asymmetrical mitotic divisions occurring in other fungal species to create genetic diversity at lower energetic expenses than mating. Overall, ploidy data of P. carinii life cycle stages shed new light on the complexity of its modes of proliferation.
Cryptosporidium parvum, a potential cause of colic adenocarcinoma
Gabriela Certad, Tramy Ngouanesavanh, Karine Guyot, Nausicaa Gantois, Thierry Chassat, Anthony Mouray, Laurence Fleurisse, Anthony Pinon, Jean-Charles Cailliez, Eduardo Dei-Cas, Colette Creusy
Infectious Agents and Cancer , 2007, DOI: 10.1186/1750-9378-2-22
Abstract: We developed a model using adult severe combined immunodeficiency (SCID) mice inoculated with Cryptosporidium parvum or Cryptosporidium muris while treated or not with Dexamethasone (Dex) in order to investigate divergences in prepatent period, oocyst shedding or clinical and histopathological manifestations. C. muris-infected mice showed high levels of oocysts excretion, whatever the chemical immunosuppression status. Pre-patent periods were 11 days and 9.7 days in average in Dex treated and untreated mice, respectively. Parasite infection was restricted to the stomach, and had a clear preferential colonization for fundic area in both groups. Among C. parvum-infected mice, Dex-treated SCID mice became chronic shedders with a prepatent period of 6.2 days in average. C. parvum-inoculated mice treated with Dex developed glandular cystic polyps with areas of intraepithelial neoplasia, and also with the presence of intramucosal adenocarcinoma.For the first time C. parvum is associated with the formation of polyps and adenocarcinoma lesions in the gut of Dex-treated SCID mice. Additionally, we have developed a model to compare chronic muris and parvum cryptosporidiosis using SCID mice treated with corticoids. This reproducible model has facilitated the evaluation of clinical signs, oocyst shedding, location of the infection, pathogenicity, and histopathological changes in the gastrointestinal tract, indicating divergent effects of Dex according to Cryptosporidium species causing infection.Cryptosporidiosis represents a major public health problem. This infection, caused by protozoa of the genus Cryptosporidium, has been reported worldwide as a frequent cause of diarrhoea, and its prevalence varies according to different regions [1]. In developed countries, massive Cryptosporidium foodborne and waterborne outbreaks have been reported. In developing countries, Cryptosporidium affects mostly children under five [2]. Furthermore, cryptosporidiosis remains a clinically signif
Cryptosporidium infection in a veal calf cohort in France: molecular characterization of species in a longitudinal study
Jér?me Follet, Karine Guyot, Hélène Leruste, Anne Follet-Dumoulin, Ourida Hammouma-Ghelboun, Gabriela Certad, Eduardo Dei-Cas, Patrice Halama
Veterinary Research , 2011, DOI: 10.1186/1297-9716-42-116
Abstract: Cryptosporidium is a genus of protozoan parasites infecting a wide range of hosts [1]. All groups of vertebrates are susceptible to Cryptosporidium infection worldwide. This parasite is the etiological agent of cryptosporidiosis, which is mainly characterized by diarrhea in humans and livestock. Massive outbreaks of enteritis in people such as in Milwaukee, Wisconsin (USA) have increased public awareness of this parasite [2]. In humans, the prevalence and severity of infection increase in immunodeficient individuals such as AIDS patients. In immunocompetent patients, the disease is self-limited [3]. No drug therapy is yet available and the high resistance of oocysts to environmental conditions and chemical treatment make cryptosporidiosis difficult to control [4]. Cattle have been considered to be a primary reservoir for Cryptosporidium oocysts for zoonotic C. parvum [5]. These animals could be a risk factor via environmental contamination from their manure being spread on farmland or their grazing on watersheds [6]. On farms, transmission of Cryptosporidium spp. can result from ingestion of contaminated food or water, by direct transmission from host to host, or through insect vectors [7]. In cattle, infection by Cryptosporidium spp. was first reported in 1971 [8]. Since vaccines have become commercially available against Escherichia coli K99, rotavirus, and coronavirus, Cryptosporidium has emerged as the main neonatal diarrheic agent in calves [9]. In farm animals, the economic impact is related to morbidity, mortality and growth retardation [10]. Among the 24 species previously described (if the three fish species are accepted without complete genetic characterization) [1,11-13], C. parvum, C. bovis, C. ryanae and C. andersoni usually infect cattle. C. parvum has zoonotic potential and is a frequent cause of human cryptosporidiosis [14]. C. bovis and C. ryanae have not been found in humans and there is only one description of C. andersoni in a patient [15]. Recen
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