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Search Results: 1 - 10 of 26260 matches for " CCl4 induced liver toxicity "
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6. Hepatoprotective activity of Coccinia indica leaves extract
B. Shyam Kumar,D. Gnanasekaran,V. Jaishree,K.P. Channabasavaraj
International Journal of Pharmaceutical and Biomedical Research (IJPBR) , 2010,
Abstract: The leaves of Coccinia indica (Family: Cucurbitaceae) are sweet, acrid, cooling, and astringent to the bowels, cures kapha and pitta. It is cultivated in Africa and Asia particularly in India used as a vegetable. A diethyl ether extract of the leaves was studied for hepatoprotective activity against carbon tetrachloride induced liver toxicity in rats. The results shownhepatoprotective activity of Coccinia indica leave extract at dose 400 mg/kg body weight was comparable with standard treatment 125 mg/kg body weight of silymarin, a known hepatoprotective drug. These data also supplement for histopathological study of rat liver section.
HEPATOPROTECTIVE EFFECT OF SOLENA AMPLEXICAULIS (TUBER) ON ACUTE CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY
Krishna Mohan G*
International Journal of Pharmacy and Technology , 2010,
Abstract: The hepatoprotective activity of methanolic extract of Solena amplexicaulis (SAME) (Lam.)Gandhi (Cucurbitaceae) at doses of 250 mg/kg and 500 mg/kg were evaluated by carbon tetrachloride(CCl4) intoxication in rats. The toxic group which received CCl4 (0.3 ml/kg of CCl4 dissolved in 1:1 ratio in olive oil by subcutaneous (s.c) alone exhibited significant increase in serum alanine aminotransferase(ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP) and total bilirubin (TB) levels. It also caused significant (P<0.001) decrease in protein levels. The groups receivedpretreatment of SAME at a dose of 500 mg/kg b.w.p.o. had controlled the AST, ALT, ALP and total bilirubin levels and the effects were comparable with standard drug (silymarin 100 mg/kg b.w.p.o).The total protein (TP) and albumin (ALB) levels were significantly increased in the animals received pretreatment of the extract at the higher dose level. The animals received pretreatment of the extractshown decreased necrotic zones and hepatocellular degeneration when compared to the liver exposed to CCl4 intoxication alone. Thus the histopathalogical studies also supported the protective effect ofthe extract.
The Potential Effect of Special Formulas on Cirrhotic Rats  [PDF]
Enayat M. Hassan, Ghada M. El-Kherbawy, Mona A. M. Ali, Omaima M. Dewidar
Food and Nutrition Sciences (FNS) , 2013, DOI: 10.4236/fns.2013.45077
Abstract:

Liver protective effect of special formulas (1 and 2) was assessed against carbon tetra chloride (CCl4) which induced liver damage in Wister albino rats. The two prepared formulas reduced the changes in body weight and liver weight caused by CCl4 in rats. The toxicity of CCl4 is related to loss in body weight and increase in liver weight in rats. The weight ratios of liver to body weight (LW/BW) significantly increased in rats treated with CCl4 followed by other groups. Formulas 1 and 2 could play an important role in improvement of hematological indices in liver cirrhosis rats. Feeding treated rats on special formulas showed improvement in liver function compared to rats fed on basal diet, reflected by significant reduction of the activity of transaminases (ALT and AST), alkaline phosphatase (ALP) and total bilirubin. There was a significant increase in total protein, albumin and globulin in serum. Significant increase in liver weight in rats treated with CCl4. There are no histopathological changes in all groups under study except for group 4 (CCl4 treated rats fed on basal diet) which orally administrated with CCl4 and had congestion of central vein and hepatic sinusoids.

密蒙花黄酮对四氯化碳所致小鼠急性肝损伤的保护作用
曹剑锋
- , 2016, DOI: 10.13982/j.mfst.1673-9078.2016.10.002
Abstract: 研究70%的乙醇浸提的密蒙花黄酮提取物对四氯化碳(CCl4)所致小鼠肝损伤的保护作用。密蒙花黄酮50、100 mg/(kg?d)剂量能显著抑制CCl4所致肝损伤小鼠肝脏指数、脾脏指数,可以使CCl4诱导的肝损伤小鼠血清中的谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、胆碱酯酶(CHE)、总胆红素(TBIL)含量下降,同时,密蒙花黄酮能提高肝脏抗氧化酶SOD的活性和GSH含量,并降低肝组织中MDA的含量,且50 mg/(kg?d)浓度密蒙花黄酮的效果更显著,接近阳性对照药物水飞蓟的作用。组织病理切片证明密蒙花黄酮可以减轻CCl4对肝组织的破坏,改善肝脏组织的病理损伤。其保护机制可能与清除自由基、抑制脂质过氧化有关。
The hepatoprotective effects of flavonoids of Buddleja officinalis Maxim. (L.) DC. (BMF) on CCl4-induced liver damage in mice were studied. The flavonoids extraction was carried out using 70% ethanol. The results showed that BMF extracts at doses 50 and 100 mg/kg/d significantly lowered the levels of serum alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), cholinesterase (ChE), lactate dehydrogenase (LDH), total bilirubin (TIBL), and hepatic malondialdehyde (MDA). In addition, the elevated values of liver index and spleen index induced by CCl4 (p<0.05) were suppressed by the BMF extracts. Moreover, the extracts enhanced superoxide dismutase (SOD) activity (p<0.05) and glutathione (GSH) levels (p<0.05) significantly compared to those of the model control group. Furthermore, 50 mg/kg/d BMF showed a stronger effect, which was close to that of silymarin (positive control drug). The results of the histopathological section demonstrated that BMF could mitigate the CCl4-induced damage in the liver tissues, thereby reducing the extent of pathological damage. The protective mechanism of BMF is possibly related to the free radicals scavenging activity and the inhibition of lipid peroxidation.
黄参茎叶多酚对四氯化碳所致小鼠急性肝损伤的保护作用
康莉娜
- , 2015, DOI: 10.13982/j.mfst.1673-9078.2015.3.004
Abstract: 研究黄参茎叶多酚对CCl4所致小鼠急性肝损伤的保护作用,探讨其对CCl4肝损伤起保护作用的有效剂量。以不同剂量的黄参茎叶多酚(150、300、600 mg/kg BW?d)给予小鼠灌胃实验进行预防,连续21 d后,腹腔注射1%的CCl4 (V/V)菜籽油0.3 mL建立CCl4诱导小鼠肝损伤模型。测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆固醇(TC)、总甘油三酯(TG)、总胆红素(TBIL)含量,以及肝组织中超氧化物岐化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)水平,并进一步观察肝脏组织病理学变化。结果表明:黄参茎叶多酚对CCl4所致小鼠肝损伤具有一定的保护作用,能显著抑制CCl4所致肝损伤小鼠肝脏指数、脾脏指数、血清ALT、AST、TBIL水平以及肝脏MDA含量的升高,能提高肝脏抗氧化酶SOD和CAT的活性,其中300 mg/kg Bw?d剂量水平效果最佳。肝组织病理学检查结果进一步证实了该结果。
The aim of this study was to investigate the protective effect of polyphenols from Sphallerocarpus gracilis stem leaves on carbon tetrachloride (CCl4)-induced hepatic injuries, and to identify its effective dosage. The mice in the treatment group were administrated with the polyphenols from Sphallerocarpus gracilis stem leaves at doses of 150, 300, and 600 mg/kg body weight (BW)/day for 21 consecutive days. The liver injury model was established by intraperitoneal injection of 3 mL 1% CCl4 rapeseed oil (V/V). The alanine transaminase (ALT), aspartate transaminase (AST), total cholesterol (TC), total triglyceride (TG), total bilirubin (TBIL) contents in serum, and superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) levels in liver were measured. Meanwhile, a hepatic pathological examination was conducted. The results of this study showed that the polyphenols from Sphallerocarpus gracilis stem leaves could, to some extent, protect mice from CCl4-induced liver injury. In addition, these polyphenols significantly prevent the increase in liver index spleen index, serum ALT, AST, TBIL, and liver MDA levels, caused by CCl4 in mice. These polyphenols could also markedly increase the activities of liver SOD and CAT. Among the tested doses, 300 mg/kg BW/day showed the best effect. Histopathological examinations further confirmed these results.
油菜蜂花粉活性成分对体外肝细胞损伤的保护作用
高丽苗
- , 2016, DOI: 10.13982/j.mfst.1673-9078.2016.9.002
Abstract: 本文通过对油菜蜂花粉的分离纯化,得到槲皮素苷QMP、山奈酚苷KMG和KMP三个化合物。通过体外培养L-02细胞,建立四氯化碳损伤模型,采用MTT实验,来研究QMP、KMG和KMP抑制四氯化碳诱导L-02细胞损伤的作用。并选用丙二醛(MDA)、超氧化物歧化酶(SOD)和乳酸脱氢酶(LDH)作为特征性指标,从氧化应激角度探究保肝作用机制。结果表明,槲皮素苷QMP处理组(200 μg/mL)细胞相对存活率分别是模型组的2.64和2.66倍,说明QMP能够明显抑制四氯化碳对L-02细胞造成的损伤,山奈酚苷KMP和KMG抗L-02细胞的损伤作用不明显。QMP、山奈酚和槲皮素显著降低细胞内MDA含量,其中300 μm剂量组MDA含量分别为模型组的0.65、0.60、0.59和0.52倍;LDH漏出率分别降低了23.82%、27.69%、30.11%和32.77%;同时SOD含量分别提高了1.45、1.61、1.58和1.65倍。这些体外实验证明了QMP具有显著的体外抗氧化和保肝作用,且其抗氧化作用是保肝作用机制之一,为开发治疗肝损伤性疾病的药物提供了选择。
Quercetin-3-O-β-D-glucosyl-(2→l)-β-glucoside (QMP), kaempferol-3,4'-di-O-β-D-glucoside (KMG), and kaempferol-3-O- β-D-glucosyl-(2→l)-β-D-glucoside (KMP) were isolated and purified from rape bee pollen. A model of CCl4-induced liver injury was established via in vitro cultivation of L-02 cells, and the protective effects of QMP, KMG, and KMP on L-02 cells with CCl4-induced injury were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The contents of malondialdehyde (MDA), superoxide dismutase (SOD), and lactic dehydrogenase (LDH) were used as the characteristic indicators to study the hepatoprotective effect in terms of oxidative stress. The relative cell survival rate of QMP-treated cells (200 μg/mL) was 2.64 and 2.66 times as much as that of the model group, respectively, suggesting that QMP can effectively prevent CCl4-induced liver injury in L-02 cells, but the hepatoprotective effects of KMG and KMP were not significant. QMP, kaempferol, and quercetin could significantly reduce intracellular MDA levels; the MDA levels of 300 μM dose groups were 0.65, 0.60, 0.59 and 0.52 times as much as that of the model group, respectively; the corresponding LDH leakage rates were reduced by 23.82%, 27.69%, 30.11%, and 32.77%, respectively; the SOD contents were increased by 1.45, 1.61, 1.58, and 1.65, respectively. The results suggest that QMP possesses significant in vitro antioxidant activity and a hepatoprotective effect, the antioxidant activity is one of the mechanisms underlying its hepatoprotective effect. This study provides a basis for developing drugs to treat diseases associated with liver injury.
Contribution to the study of collagen degradation
Cavalcanti, Andrea;Barbosa Jr., Aryon A.;Andrade, Zilton A.;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2002, DOI: 10.1590/S1676-24442002000400012
Abstract: the rate and morphology of collagen degradation were comparatively analyzed in rats with "early" and "late" carbon tetrachloride-induced hepatic fibrosis, during three different periods after discontinuation of the drug. treatment with ccl4 lasted for eight weeks for the group of early fibrosis and 12 weeks for the late fibrosis group. fibrosis gradually disappeared, but when quantitative methods were applied, the amount of collagen degradation and removal in early and late fibrosis did not reach statistical significance. the light microscope and ultrastructural changes were also qualitatively similar in both cases. probably some long-lasting effects to the liver, induced by the drug, prevented early hepatic fibrosis from undergoing the characteristic ultrastructural changes seen during "acute" collagen degradation, as compared to other experimental models of hepatic fibrosis, after the causal agent is totally removed. present findings suggest that not only the age of fibrosis but also the overall damage inflicted to the organ are limiting factors in the process of collagen degradation and removal.
Research: STUDY THE POSSIBLE HEPATOPROTECTIVE EFFECT OF DIFFERENT DOSES OF Ammi majus SEEDS' EXTRACT AGAINST CCl4 INDUCED LIVER DAMAGE IN RATS
Shihab Hattab Mutlag,Dawser K Ismael,Nada N Al-Shawi*
Pharmacie Globale : International Journal of Comprehensive Pharmacy , 2011,
Abstract: Liver is considered as the major organ responsible for conducting various metabolic processes and according to it’s highly exposed to toxic effect of different xenobiotics predisposing to many types of diseases and disorders. The role of plant with antioxidant activity in the treatment and prevention of chemical-induced liver damage was extensively studies. Ammi majus show antioxidant effect their use in diabetic nephropathy and myocardial injury due to the presence of different active constituent such as quercetine, marmesinin, kempefrol and other compounds that inhibit cytochrome p450 such as xanthotoxin bergapten, imperatorin and isoimpinellin. Accordingly, this study was designed to evaluate the hepatoprotective effect of the aqueous solution of ethanolic extract of the Ammi majus against carbon tetrachloride (CCl4) induced liver damage in rats. Eighty adult rats of both sex divided into four groups allocated as follows: Group I- (negative control), rats received D.W 2ml/kg for 14 days. Group II- rats treated with single oral daily dose Ammi majus seeds extract 16mg/rat/day alone for 14 days. The animals of groups I and II were sacrificed by anesthetic ether on the day 15. Group III- (positive control) rats received single oral daily dose of 2ml/kg/day D.W. for 14 days and at the day 15, the animals received single oral dose of CCl4, the animals were sacrificed by anesthetic ether 24 hr after CCl4 administration. Groups IV (A, B, C, D and E) received either (1mg or 2mg or 4mg or 8mg or 16mg/rat/day), respectively for 14 days of Ammi majus ethanolic extract then at the day 15 they received single dose of CCl4 then sacrificed after 24 hours after CCl4 administration. Rats' livers were obtained for preparation of tissue homogenate to evaluate MDA & GSH in the hepatic homogenate as indicator of lipid peroxidation. Blood samples were collected by intra-cardiac puncture, and utilized for evaluating serum enzymes activities manifested by aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in addition for assessing total serum Bilirubin (TSB). Analysis of data revealed significant amelioration of oxidative stress in rats pre-treated with different doses of Ammi majus extract (4mg,8mg and 16mg/rat/day for 14days) compared to group III of animals intoxicated by CCl4 as evidenced by lowering MDA contents and elevation of GSH levels in liver tissue homogenate but the levels still significantly different compared to controls. Elevation of serum activities of ALT, AST and ALP, in addition to TSB levels as a results of t
Contribution to the study of collagen degradation
Cavalcanti Andrea,Barbosa Jr. Aryon A.,Andrade Zilton A.
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2002,
Abstract: The rate and morphology of collagen degradation were comparatively analyzed in rats with "early" and "late" carbon tetrachloride-induced hepatic fibrosis, during three different periods after discontinuation of the drug. Treatment with CCl4 lasted for eight weeks for the group of early fibrosis and 12 weeks for the late fibrosis group. Fibrosis gradually disappeared, but when quantitative methods were applied, the amount of collagen degradation and removal in early and late fibrosis did not reach statistical significance. The light microscope and ultrastructural changes were also qualitatively similar in both cases. Probably some long-lasting effects to the liver, induced by the drug, prevented early hepatic fibrosis from undergoing the characteristic ultrastructural changes seen during "acute" collagen degradation, as compared to other experimental models of hepatic fibrosis, after the causal agent is totally removed. Present findings suggest that not only the age of fibrosis but also the overall damage inflicted to the organ are limiting factors in the process of collagen degradation and removal.
Hepatoprotective and Antioxidant Effects of Licorice Extract against CCl4-Induced Oxidative Damage in Rats
Hai Zhong Huo,Bing Wang,Yong Kang Liang,Yong Yang Bao,Yan Gu
International Journal of Molecular Sciences , 2011, DOI: 10.3390/ijms12106529
Abstract: Licorice has been used in Chinese folk medicine for the treatment of various disorders. Licorice has the biological capabilities of detoxication, antioxidation, and antiinfection. In this study, we evaluated the antihepatotoxic effect of licorice aqueous extract (LE) on the carbon tetrachloride (CCl 4)-induced liver injury in a rat model. Hepatic damage, as reveled by histology and the increased activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) activities, and decreased levels of serum total protein (TP), albumin (Alb) and globulin (G) were induced in rats by an administration of CCl 4 at 3 mL/kg b.w. (1:1 in groundnut oil). Licorice extract significantly inhibited the elevated AST, ALP and ALT activities and the decreased TP, Alb and G levels caused by CCl 4 intoxication. It also enhanced liver super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), Glutathione S-transferase (GST) activities and glutathione (GSH) level, reduced malondialdehyde (MDA) level. Licorice extract still markedly reverses the increased liver hydroxyproline and serum TNF-α levels induced by CCl 4 intoxication. The data of this study support a chemopreventive potential of licorice extract against liver oxidative injury.
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