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Search Results: 1 - 10 of 608571 matches for " C.A.A.;Guimar?es "
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Vitrifica o de ovócitos imaturos de bovinos utilizando etilenoglicol associado à trehalose e polivinilpirrolidona
Souza M.R.,Costa E.P.,Torres C.A.A.,Guimares J.D.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2003,
Abstract: Avaliaram-se os efeitos da vitrifica o de ovócitos imaturos de bovinos utilizando o etilenoglicol (EG) associado à trehalose e à polivinilpirrolidona (PVP). Utilizaram-se ovócitos provenientes de ovários de vacas abatidas em matadouro, distribuídos aleatoriamente em três tratamentos (T). TI - ovócitos n o desnudados e n o congelados, TII - ovócitos vitrificados com cumulus oophorus e TIII - ovócitos desnudados vitrificados. A percentagem de ovócitos recuperados e ovócitos com morfologia normal após a vitrifica o foi diferente entre TII e TIII (92,2 e 72,6%; 79,0 e 63,6%, respectivamente). Os ovócitos normais foram cultivados à 38,5oC em atmosfera de 5% de CO2 por 24 horas. Após o cultivo, os ovócitos foram fecundados e os embri es cultivados in vitro por sete dias. Foram encontradas diferen as entre tratamentos quanto às taxas de matura o nuclear, fecunda o e clivagem (83,9, 70,0 e 44,0%; 17,5, 23,7 e 5,1%; 0,0, 0,0 e 0,0% para os tratamentos I, II e III, respectivamente). Apenas no TI foram obtidas mórulas e blastocistos (21,4%). Os procedimentos de vitrifica o, segundo os protocolos utilizados, n o s o indicados para a criopreserva o de ovócitos imaturos de bovinos.
Utiliza??o de gonadotrofina cori?nica humana e cipionato de estradiol associado ao dispositivo de libera??o controlada de drogas para sincroniza??o de ovula??o em cabras da ra?a Saanen
Maffili, V.V.;Torres, C.A.A.;Pontes, R.A.M.;Guimares, J.D.;Prosperi, C.P.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2005, DOI: 10.1590/S0102-09352005000200012
Abstract: saanen nuliparous female goats were distributed in two experimental groups of eight animals each to synchronize the ovulation. on day zero, all animals were treated with controlled internal drug release (cidr-g?), dinoprost (5mg) and estradiol cipionate (ec; 1mg). on day 4, all animals were treated with ecg (250iu), and on day 5 the cidr-g? was removed. group 1 (t1) received 1mg of ec and group 2 (t2) received 250iu of hcg, 24 and 30 hours after cidr-g? removal, respectively. onset of estrus was observed in 7 and 8 goats of t1 and t2 groups, respectively. hcg treated goats remained in estrus for 42±6.9 hours, whereas ec-treated goats remained in estrus for 45±5.5 hours (p>0.05). none of the studied ovulatory characteristics were affected by treatments. the intervals between cidr-g? removal and ovulation in t1 and t2 groups were similar. both hcg and ec were equally efficient on inducing ovulation synchronically.
Efeito de fontes de óleo e níveis de suplementa??o de vitamina E na ra??o sobre as características físicas e morfológicas do sêmen in natura de suínos
Moraes, E.A.;Torres, C.A.A.;Guimares, J.D.;Murgas, L.D.S.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2010, DOI: 10.1590/S0102-09352010000300004
Abstract: the addition of oil sources and dietary vitamin e supplementation was evaluated on the characteristics of fresh boar sperm. twenty-four mature dalboar 85 boars of proven fertility and in routine semen production for artificial insemination were randomly distributed in a factorial arrangement 2 x 3, with two oil sources, soy and salmon, and three antioxidant levels: 150, 300, and 450mg/kg of vitamin e. volume, total motile sperm, hyposmotic swelling test, percentage of live cell, and morphology did not differ (p<0.05) between the animals from the treatments. salmon oil improved (p<0.05) sperm vigor. the vitamin e added to the diet improved (p<0.05) the sperm concentration, and there was no difference between the oil sources (p>0.05). salmon oil treatment showed the lowest (p<0.05) concentration of total antioxidants in the semen. vitamin e and concentration of total antioxidant showed a linear effect (p<0.05). the semen vigor and the concentration of total antioxidants were improved by oil sources, but the sperm concentration was dependent on the levels of vitamin e. therefore, salmon oil can be used in the diet of male pigs.
Potencial reprodutivo de touros da ra?a Nelore submetidos a diferentes propor??es touro: vaca
Santos, M.D.;Torres, C.A.A.;Ruas, J.R.M.;Guimares, J.D.;Silva Filho, J.M.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2004, DOI: 10.1590/S0102-09352004000400011
Abstract: this study evaluated the reproductive potential of 20 nelore bulls, submitted to different bull:cow ratio. correlations among the serum testosterone concentration, scrotal circumference, sperm quality and libido were also estimated. the bulls selected by andrologic evaluation and the libido test were alloted to the following bull:cow ratio (treatments): 1:25 (t1), 1:50 (t2), 1:75 (t3) e 1:100 (t4), with five replicates per treatment. blood samples were collected to determine serum testosterone concentration. semen physical and morphologic characteristics, scrotal circumference and libido of the bulls did not differ among treatments. the pregnancy rate of the cows was not affected by treatments (means of 42.1, 66.5 and 71.0 % for breeding season of 30, 60 and 90 days, respectively). the testosterone concentration was not affected by treatments and the mean serum testosterone concentration was 4.04ng/ml, which peaked at 9am. the correlations between the libido, semen physical and morphologic characteristics, and the scrotal circumference of the bulls were low. nelore bulls with good sperm quality and libido supported a large number of cows, and the bull:cow ratio of 1:100 did not reduce the pregnancy rate.
Vitrifica??o de ovócitos imaturos de bovinos utilizando etilenoglicol associado à trehalose e polivinilpirrolidona
Souza, M.R.;Costa, E.P.;Torres, C.A.A.;Guimares, J.D.;Fagundes, L.M.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2003, DOI: 10.1590/S0102-09352003000500011
Abstract: this study aimed to evaluate the effects of vitrification procedure of immature bovine oocytes using ethylene glycol (eg) associated with trehalose and polivinylpyrrolidone on the percentage of recovered oocytes with normal morphology and nuclear maturation, fecundation and cleavage rates for in vitro cultivated embryos. ovary oocytes of slaughtered cows were randomly allotted to three treatments (t): ti - oocytes neither undenuded nor vitrified, tii - vitrified oocytes with cumulus oophorus, tiii - undenuded vitrified oocytes. the percentage of recovered oocytes and oocytes with normal morphology after vitrification was different for tii and tiii (92.2 and 72.6%, 79.0 and 63.3% for tii and tiii, respectively). all normal oocytes were cultivated at 38.5oc in atmosphere with 5% co2 for 24 hours. after culture, the oocytes were fecundated and the embryos were cultivated in vitro for seven days. the nuclear maturation, fecundation and cleavage rates for ti, tii and tiii were different (83.9, 70.0 and 44.0%, 17.5, 23.7 and 5.1%, 0.0, 0.0 and 0.0% for ti, tii and tiii, respectively). morulas and blastocysts were obtained only for ti (21.4%). these results indicate that the protocol used for vitrification procedure is not recommended for cryopreservation of immature bovine oocytes.
Libido de touros Nelore: efeito da propor??o touro: vaca sobre a taxa de gesta??o
Santos, M.D.;Torres, C.A.A.;Guimares, J.D.;Ruas, J.R.M.;Carvalho, G.R.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2003, DOI: 10.1590/S0102-09352003000300008
Abstract: the effect of the libido of the bulls on pregnancy rates at 30, 60 and 90 days of the breeding season was studied, and the correlations between the serum testosterone levels, seminal quality and libido were estimated. twelve bulls selected by andrologic evaluation and by the libido test, were randomly allotted to: treatments t1 and t2, three low libido bulls and three high libido bulls, respectively, in the bull:cow proportion of 1:75; and treatments t3 and t4, three low libido bulls and three high libido bulls, respectively, in the proportion of 1:100. no differences among bulls based on the serum testosterone levels were observed. bulls with high libido (proportion 1:75) provided larger pregnancy rate at 60 and 90 days (90.6 and 94.6%, respectively) of the breeding season, than low libido bulls in the same proportion (80.0 and 86.2%, respectively). the libido of the bulls in the proportion 1:100 had no effect on the pregnancy rates. the effect of bull:cow proportion on the pregnancy rate was not significant and no correlations between libido, seminal quality, scrotal circumference and serum testosterone levels were observed.
Libido de touros Nelore: efeito da propor o touro: vaca sobre a taxa de gesta o
Santos M.D.,Torres C.A.A.,Guimares J.D.,Ruas J.R.M.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2003,
Abstract: Estudou-se a influência da libido de touros sobre as taxas de gesta o de vacas aos 30, 60 e 90 dias de esta o de monta, e avaliaram-se as correla es entre concentra o de testosterona, qualidade seminal e libido. Foram selecionados 12 touros (exame andrológico e teste da libido), distribuídos nos tratamentos: T1 e T2, três com libido baixa e três com libido alta, respectivamente, na propor o touro:vaca de 1:75, e T3 e T4, três com libido baixa e três com libido alta, respectivamente, na propor o de 1:100. N o houve diferen a entre touros quanto à concentra o de testosterona no soro. Touros com alta libido na propor o 1:75 proporcionaram maior taxa de gesta o aos 60 e 90 dias (90,6 e 94,6%, respectivamente) da esta o de monta em rela o aos de libido baixa na mesma propor o (80,0 e 86,2%, respectivamente). A libido dos touros na propor o 1:100 n o influenciou a taxa de gesta o. N o se observou efeito da propor o touro:vaca sobre a taxa de gesta o e n o se observou correla o entre libido, qualidade seminal, circunferência escrotal e concentra o sérica de testosterona.
Taxa de gesta??o em cabras Alpinas e Saanen tratadas com hCG no terceiro dia após o estro
Prosperi, C.P.;Torres, C.A.A.;Guimares, J.D.;Bruschi, J.H.;Leite, P.A.G.;Maffili, V.V.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2006, DOI: 10.1590/S0102-09352006000200007
Abstract: the experimental objective was to evaluate the effect of human chorionic gonadotropin (hcg) administration on pregnancy rate and gestation length and on serum concentration of progesterone (p4) in alpine (83) and saanen (60) dairy goats induced by artificial photoperiod to enter the breeding season. animals were randomly allocated to two treatments following estrus: in treatment 1 (t1) goats received 1ml saline solution and in treatment 2 (t2) animals received 250iu of hcg in 1ml saline intramuscularly during the third day after estrus. pregnancy rates detected by ultrasound technology 35 days after mating did not differ significantly between treatments (p>0.05). they equaled 80.4 and 70.3% for alpine goats and 72.7 and 81.5% for saanen goats in t1 and t2, respectively. overall pregnancy rates were 77.2% and 75.0% for the controls and treated animals, respectively. gestation length was not affected by the hcg treatment. blood samples were collected from 5 alpine does from each treatment the day of estrus (day 0) and on days 3, 8, 15, 21, 42 and 60 after estrus, for determination of progesterone concentration in plasma. means were 5.84 and 5.76ng/ml for the control and treated does, respectively, and were not affected by treatment (p>0.05), although concentrations did differ among collection days (p<0.05). the administration of hcg the third day after estrus did not affect pregnancy rate or p4 concentration and had no beneficial effect on reproduction in the does.
Dinamica folicular em cabras da ra?a Toggenburg em lacta??o tratadas ou n?o com somatotropina bovina recombinante
Amorim, E.A.M.;Torres, C.A.A.;Amorim, L.S.;Fonseca, J.F.;Bruschi, J.H.;Guimares, J.D.;Carvalho, G.R.;Alves, N.G.;Cecon, P.R.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2007, DOI: 10.1590/S0102-09352007000600024
Abstract: the effect of the r-bst injection was evaluated on the goats estrous cycles. twenty-four toggenburg does were used in two treatments: t1 (n=12) treated with r-bst; and t2 (n=12) treated with saline solution (control). after the first r-bst injection, the estrous cycles were checked between two estrus. the estrous cycle length and interovulatory period of the goats did not differ between treatments (p>0.05). estrous cycles with two, three and four waves of follicular growth were observed. the number of waves during the estrous cycle were not affected by the r-bst treatment (p>0.05). the number of 3mm follicles was different between t1 and t2 (p<0.05) for estrous cycles with two (15.3±1.2 x 8.1±2.0), three (12.2±0.8 x 8.3±1.9) or four (12.7±1.7 x 8.8±2.5) waves. the dominant follicle of the second wave was smaller (p<0.05) than the one of the first and third waves and smaller than the one of the ovulatory wave. the progesterone concentration during the estrous cycle of the animals did not differ (p>0.05) between treatments. the r-bst did not affect the ovarian response during the estrous cycle. the r-bst not affected the follicular dynamic of toggenburg lactating does, but increased the number of emerging follicles >3mm during the estrous cycle.
Sincroniza??o de estro com CIDR reutilizado em cabras lactantes da ra?a Toggenburg tratadas com somatotropina bovina recombinante (r-bST)
Amorim, E.A.M.;Torres, C.A.A.;Fonseca, J.F.;Amorim, L.S.;Maffili, V.V.;Bruschi, J.H.;Guimares, J.D.;Cecon, P.R.;Alves, N.G.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2008, DOI: 10.1590/S0102-09352008000100008
Abstract: the response of goats treated with r-bst in the estrous synchronization protocol was evaluated. twenty-six toggenburg females were divided in two treatments: t1 (n=13) treated with four injections of 250mg r-bst, at 14 days intervals, and t2 (n=13) treated with saline solution (control). the intravaginal device previously used by five days was inserted (day 0) one week after the last injection of r-bst and pgf2a (22.5μg) was injected in the animals of both treatments. the device was removed on day 6. all the females in estrus were bred by fertile bucks. the percentage of animals in estrus and the pregnancy rate were: 76.9 and 70, and 84.6 and 72.7% for goats in t1 and t2, respectively. the estrous cycle length; the interval from treatment to the onset of estrus; the ovulation number; and the intervals from the onset of estrus to ovulation, end of estrus to ovulation, and from device removal to ovulation were not affected by r-bst injection. the r-bst did not affect the diameter of the ovulatory follicles (p>0.05). progesterone concentration showed similar values (p>0.05) among the goats of t1 and t2 during the treatment with device. the r-bst administration did not affect estrous synchronization.
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