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Search Results: 1 - 10 of 317630 matches for " Célia Sperandéo;Capelletti "
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Role of metabolic control on diabetic nephropathy
Macedo Célia Sperandéo,Capelletti Sonia Maria,Mercadante Maria Cecília Salgado,Padovani Carlos Roberto
Acta Cirurgica Brasileira , 2002,
Abstract: OBJECTIVE: The aim of this investigation was studying the influence of glucose metabolic control on diabetic nephropathy. The authors observed the effect of acarbose, insulin, and both drugs on the metabolic control and development of mesangial enlargement of kidney glomeruli in alloxan-diabetic rats. METHODS: Five groups of Wistar rats were used: normal rats (N), non-treated alloxan-diabetic rats (D), alloxan-diabetic rats treated with acarbose (AD), alloxan-diabetic rats treated with insulin (ID), and alloxan-diabetic rats treated with insulin plus acarbose (IAD). The following parameters were evaluated: body weight; water and food intake; diuresis; blood and urine glucose levels; and the kidney lesions: mesangial enlargement and tubule cell vacuolization. Renal lesions were analysed using a semi-quantitative score 1, 3, 6, 9, and 12 months after diabetes induction. RESULTS: Diabetic rats showed a marked increase of glycemia, urinary glucose levels, diuresis, water and food intake, and weight loss, while the treated diabetic rats showed significant decreased levels of these parameters. The most satisfactory metabolic control was that of diabetic rats treated with acarbose + insulin. There was a significant mesangial enlargement in diabetic rats compared to normal rats from the third up to the 12th month after diabetes induction, with a significant difference between the animals treated with acarbose + insulin and non-treated diabetic rats. A difference between the animals treated with acarbose or insulin alone and non-treated diabetics rats was not seen. CONCLUSIONS: The authors discuss the results stressing the role of diabetic metabolic control in the prevention of diabetic nephropathy.
Role of metabolic control on diabetic nephropathy
Macedo, Célia Sperandéo;Capelletti, Sonia Maria;Mercadante, Maria Cecília Salgado;Padovani, Carlos Roberto;Spadella, César Tadeu;
Acta Cirurgica Brasileira , 2002, DOI: 10.1590/S0102-86502002000600003
Abstract: objective: the aim of this investigation was studying the influence of glucose metabolic control on diabetic nephropathy. the authors observed the effect of acarbose, insulin, and both drugs on the metabolic control and development of mesangial enlargement of kidney glomeruli in alloxan-diabetic rats. methods: five groups of wistar rats were used: normal rats (n), non-treated alloxan-diabetic rats (d), alloxan-diabetic rats treated with acarbose (ad), alloxan-diabetic rats treated with insulin (id), and alloxan-diabetic rats treated with insulin plus acarbose (iad). the following parameters were evaluated: body weight; water and food intake; diuresis; blood and urine glucose levels; and the kidney lesions: mesangial enlargement and tubule cell vacuolization. renal lesions were analysed using a semi-quantitative score 1, 3, 6, 9, and 12 months after diabetes induction. results: diabetic rats showed a marked increase of glycemia, urinary glucose levels, diuresis, water and food intake, and weight loss, while the treated diabetic rats showed significant decreased levels of these parameters. the most satisfactory metabolic control was that of diabetic rats treated with acarbose + insulin. there was a significant mesangial enlargement in diabetic rats compared to normal rats from the third up to the 12th month after diabetes induction, with a significant difference between the animals treated with acarbose + insulin and non-treated diabetic rats. a difference between the animals treated with acarbose or insulin alone and non-treated diabetics rats was not seen. conclusions: the authors discuss the results stressing the role of diabetic metabolic control in the prevention of diabetic nephropathy.
Reduction of podocytes number in late diabetic alloxan nephropathy: prevention by glycemic control
Macedo, Célia Sperandéo;Lerco, Mauro Masson;Capelletti, S?nia Maria;Silva, Reinaldo José;Pinheiro, Daniela de Oliveira;Spadella, César Tadeu;
Acta Cirurgica Brasileira , 2007, DOI: 10.1590/S0102-86502007000500003
Abstract: purpose: to determine podocyte number and gbm thickness in diabetic rats either under glycemic control or without glycemic control at 6 and 12 months after diabetes induction. methods: 100 wistar rats weighing 200-300g were divided into 6 groups: normal group (n6 and n12- 25 rats); diabetic group (d6 and d12- 25 rats), diabetic treated group ( dt 6 and dt 12- 25 rats) on insulin 1,8- 3,0 iu/kg associated with acarbose (50mg to 100g of food) daily mixed in chow. alloxan was injected intravenously in a dose of 42 mg/kg of weight. body weight, waterintake, 24-h diuresis, glycemia and glucosuria were determined before induction, 7 and 14 days after induction and monthly thereafter. treatment started at day 14. three groups were sacrificed at 6 months (n6,d6, dt6) and 3 groups at 12 months (n12, d12, dt12) with the renal tissue being prepared for electron microscopy. results: glycemia in dt6¨and in dt12 was significantly different from that in d6 and d12 rats and similar to that in n6 and n12 animals. the number of podocytes in dt6 was not different from that in n6 and d6 (median = 11); the number of podocytes in dt12 (median = 11) differed from that in d12 (median = 8), but not from that in n12 (median = 11). gbm thickness in d6 (0.18 micrometers) was lower than in d12 (0.29 micrometers); while in dt6 (0.16 micrometers) it was lower than in d6 (0.18 micrometers). in dt12 (0.26 micrometers), it was lower than in d12 (0.29 micrometers). conclusion: the control of hyperglycemia prevented gbm thickening in early and late (12 mo) alloxan diabetic nephropathy and podocyte number reduction.
Padr?es morfológicos de les?o glomerular e correla??o com achados clinicolaboratoriais de 43 crian?as com síndrome nefrótica
Riyuzo, Márcia Camega?ava;Viero, Rosa Marlene;Macedo, Célia Sperandéo;Bastos, Herculano Dias;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2004, DOI: 10.1590/S1676-24442004000500009
Abstract: objectives: to evaluate the association between clinical features and laboratory findings with the morphological changes in children with nephrotic syndrome. methods: the data were obtained from medical records of 43 children with nephrotic syndrome submitted to renal biopsy. results: twenty-eight patients were male (65.1%), aged 1.4-12 years (mean 4.7 ± 3,2). forty-two patients (97,7%) presented edema, 83.7% oliguria and 32.5% hypertension. the mean of proteinuria was 15.3g/1.73m2 bsa per day and 55.8% presented microscopic hematuria. renal biopsies showed: proliferative mesangial glomerulonephritis (pmgn) in 37.2%, focal segmental glomerulosclerosis (fsgs) in 27.9%, minimal change disease (mcd) in 25.6%, membranoproliferative glomerulonephritis (mpgn) in 7% and membranous glomerulonephropathy (mgn) in 2.3%. twenty-six patients (60.5%) were steroid-resistant. age, sex, hypertension, oliguria, serum urea and creatinine showed no statistically significant change between the patients with pmgn, fsgs and mcd. the patients with pmgn and fsgs showed higher frequency of microscopic hematuria (p < 0.003 and p < 0.03, respectively) and those with fsgs higher level of proteinuria. the patients with mcd were steroid responsive (p < 0.001 versus fsgs and p = 0.047 versus pmgn). conclusion: age, sex, hypertension, oliguria, serum urea and creatinine did not help to distinguish between the patients with pmgn, fsgs and mcd. the patients with mcd were steroid responsive and less likely to have microscopic hematuria. patients with fsgs presented higher level of proteinuria.
Altera??es cognitivas em escolares de classe socio-econ?mica desfavorecida: resultados de interven??o psicopedagógica
Macedo, Célia Sperandéo;Andreucci, Lívia Christina;Montelli, Terezinha de Cresci Braga;
Arquivos de Neuro-Psiquiatria , 2004, DOI: 10.1590/S0004-282X2004000500021
Abstract: sixty-three school-age children of low socioeconomic status and exposed to adverse environmental factors (malnutrition, familiar distress and low familiar incomes) were submitted to neuropsychological tests to investigate possible cognitive impairments. classical neuropsychological test battery was employed (raven test, bender gestalt copy of complex figures, draw-a-man goodenough test). low intellectual level was found on 30% and 74% showed higher cognitive disorders (visuoperceptual skills and/or perseverations and/or global shapes perception and/or draw-a-man disturbances). these children attended to a school with semi-boarding regimen which receives children under personnel and social adverse factors. school program was enriched with learning activity program based on piaget and psychomotor exercises based on lambert for at least one year. they also had some other activities, as painting, singing, computer training, english and spanish classes. twenty children were newly accepted and 43 attended at school for one, two or three years. we found significant correlations (pc0.05) between superior intellectual performances, bigger periods of attendance at school and methods for cognitive development. there was no association between other brain cognitive functions examined, the attendance to the teaching programs and the years of permanence at school.
The number of podocyte and slit diaphragm is decreased in experimental diabetic nephropathy
Lerco Mauro Masson,Macedo Célia Sperandéo,Silva Reinaldo José,Pinheiro Daniela de Oliveira
Acta Cirurgica Brasileira , 2006,
Abstract: PURPOSE: To determine the number of podocyte, slit diaphragms, slit diaphragm extensions and GBM thickness in diabetic nephropathy. METHODS: Sixty "Rattus Wistar"of both sexes weighing 200-300g were divided in two experimental groups: normal group 10 animals, and alloxan diabetic rats - 50 animals. Alloxan was administered in a single IV dose of 42mg/kg body weight. Body weight, water and food intake, diuresis, and blood and urine glucose were determined in both groups before alloxan injection and two weeks, six and twelve months after alloxan injection. Proteinuria was measured at 12 months in both groups. After 12 months animals were sacrificed, and the right kidney processed for electron microscopy. RESULTS: Clear clinical and laboratory signs of severe diabetes were seen, in all alloxan-diabetic rats at all follow-up times. Glomerular basement membrane (GBM) thickening, podocyte number, and slit diaphragm number and extension were determined. GBM of all diabetic rats was significantly thicker (median=0.29μm; semi-interquartile range=0.065μm) than in the normal rats (0.23μm; 0.035μm). Diabetic rat podocyte number (8; 1), slit diaphragm number (4; 1), and slit diaphragm extension (0.021μm; 0.00435μm) were significantly lower than in normal rats (11; 1) and (7; 1.5), and (0.031μm; 0.0058μm). Diabetic rat proteinuria (0.060mg/24h; 0.037mg/24h) was higher than in normal rats (0.00185mg/24h; 0.00055mg/24h). CONCLUSION: Experimental diabetes is associated with significant (p<0.05) changes in podocyte foot process, slit number, slit diaphragm extension, and GBM thickness.
Estudo comparativo entre cinco diferentes tratamentos sobre as altera??es clínicas e laboratoriais do rato diabético induzido pela aloxana
Spadella, César Tadeu;Macedo, Célia Sperandéo;Machado, José Lúcio Martins;Schellini, Silvana Artioli;Padovanni, Carlos Roberto;
Acta Cirurgica Brasileira , 2005, DOI: 10.1590/S0102-86502005000100008
Abstract: purpose: the long-term effects of five different treatments of diabetes were evaluated in alloxan-induced diabetic rats. methods: seven experimental groups, with 50 rats each (gn normal control; gd untreated diabetic control; gi, ga, gia treated groups with insulin, acarbose, and insulin plus acarbose, respectively; gtil, gtpd treated groups with islet of langerhans and pancreas transplantation) were studied. clinical (body weight, water intake, food intake and urine output) and laboratory (blood and urinary glucose, and plasma insulin) parameters were analyzed at the beginning of the study, and after 1, 3, 6, 9 and 12 months of follow-up. results: mortality was observed in all groups, except gn, during 12 months (gd= 50%; gi= 20%; ga= 26%; gia= 18%; gtil= 4%; gtpd= 20%). rats from the gd, gi, and gia groups died due to metabolic or hydrossaline disbalance, and/or pneumonia, diarrhoea, and cachexy. all deaths observed in gtil and gtpd groups were in decorrence of technical failure at the immediate postoperative, until 72h. animals from the gi, ga and gia had significative improving of the clinical and laboratory parameters (p < 0,05) observed in diabetic rats, being the efficacy of theses treatments equal. however, rats from the gtil and gtpd groups had better control of these parameters than gi, ga, and gia groups. transplanted rats had complete restoration, at the normal levels, of all analyzed variables (p<0,01). conclusions: conventional treatments with insulin, acarbose, and insulin plus acarbose improved the severe diabetic state of the alloxan-diabetic rats, but pancreas and islet transplantation have a better performance for treatment of diabetes.
The number of podocyte and slit diaphragm is decreased in experimental diabetic nephropathy
Lerco, Mauro Masson;Macedo, Célia Sperandéo;Silva, Reinaldo José;Pinheiro, Daniela de Oliveira;Spadella, César Tadeu;
Acta Cirurgica Brasileira , 2006, DOI: 10.1590/S0102-86502006000200006
Abstract: purpose: to determine the number of podocyte, slit diaphragms, slit diaphragm extensions and gbm thickness in diabetic nephropathy. methods: sixty "rattus wistar"of both sexes weighing 200-300g were divided in two experimental groups: normal group 10 animals, and alloxan diabetic rats - 50 animals. alloxan was administered in a single iv dose of 42mg/kg body weight. body weight, water and food intake, diuresis, and blood and urine glucose were determined in both groups before alloxan injection and two weeks, six and twelve months after alloxan injection. proteinuria was measured at 12 months in both groups. after 12 months animals were sacrificed, and the right kidney processed for electron microscopy. results: clear clinical and laboratory signs of severe diabetes were seen, in all alloxan-diabetic rats at all follow-up times. glomerular basement membrane (gbm) thickening, podocyte number, and slit diaphragm number and extension were determined. gbm of all diabetic rats was significantly thicker (median=0.29μm; semi-interquartile range=0.065μm) than in the normal rats (0.23μm; 0.035μm). diabetic rat podocyte number (8; 1), slit diaphragm number (4; 1), and slit diaphragm extension (0.021μm; 0.00435μm) were significantly lower than in normal rats (11; 1) and (7; 1.5), and (0.031μm; 0.0058μm). diabetic rat proteinuria (0.060mg/24h; 0.037mg/24h) was higher than in normal rats (0.00185mg/24h; 0.00055mg/24h). conclusion: experimental diabetes is associated with significant (p<0.05) changes in podocyte foot process, slit number, slit diaphragm extension, and gbm thickness.
Progression of nephropathy after islet of langerhans transplantation in alloxan-induced diabetic rats
Spadella, César Tadeu;Mercadante, Maria Cecília Salgado;Breim, Luiz Carlos;Macedo, Célia Sperandéo de;Bacchi, Carlos Eduardo;Macedo, Arthur Roquete de;
Acta Cirurgica Brasileira , 1997, DOI: 10.1590/S0102-86501997000100003
Abstract: we studied the effects of islet of langerhans transplantation (it) on the kidney lesions of rats with alloxan-induced diabetes. forty-five inbred male lewis rats were randomly assigned to 3 experimental groups: group gl included 15 non-diabetic control rats (nc), group git included 15 alloxan-induced diabetic rats (dc), and group iii included 15 alloxan-induced diabetic rats that received pancreatic islet transplantation prepared by nonenzymatic method from normal donor lewis rats and injected into the portal vein (it). each group was further divided into 3 subgroups of 5 rats which were sacrificed at 1, 3, and 6 months of follow-up, respectively. clinical and laboratorial parameters were recorded in the mentioned periods in the 3 experimental groups. for histology, the kidneys of all rats of each subgroup were studied and 50 glomeruli and 50 tubules of each kidney were analyzed using light microscopy by two different investigators in a double blind study. the results showed progressive glomerular basement membrane thickening (gbmt), mesangial enlargement (me), and bowman's capsule thickening (bct) in the 3 experimental groups throughout the follow-up. these alterations were significantly more severe in dc rats at 6 months when compared to nc rats (p < 0.01). however, the degree of gbmt, me, and bct observed in dc rats was not statistically different from it rats at 1, 3, and 6 months. in addition, armanni-ebstein lesions of the tubules (ae) and tubular lumen protein (pro) observed in dc rats were also observed in it rats all over the study. these lesions were never present in nc rats. we conclude that it did not prevent progression of kidney lesions in alloxan-induced diabetic rats within 6 months after transplantation.
Prevalência de press?o arterial elevada em crian?as e adolescentes do ensino fundamental
Rinaldi, Ana Elisa M.;Nogueira, Paulo César K.;Riyuzo, Márcia Camega?ava;Olbrich-Neto, Jaime;Gabriel, Gleice Fernanda C. P.;Macedo, Célia Sperandéo;Burini, Roberto Carlos;
Revista Paulista de Pediatria , 2012, DOI: 10.1590/S0103-05822012000100012
Abstract: objective: to assess the prevalence of elevated blood pressure in schoolchildren and adolescents and the association of blood pressure with anthropometric measures. methods: this cross-sectional study, conducted in three schools in botucatu, brazil, collected blood pressure (bp) measurements taken at three different time points and anthropometric data: weight, height, body mass index (bmi), arm circumference, waist circumference, triceps and subscapular skinfolds. blood pressure was measured using the auscultation method, and children were classified into two groups: pre-hypertension or hypertension for values between the 90th and 95th percentiles or above the 95th percentile. data were compared according to sex using the student's t test. the pearson correlation coefficient was used to evaluate the association between blood pressure and anthropometric data. to evaluate blood pressure, the z score according to bmi percentile categories, one-factor analysis of variance (anova) and the tukey post hoc test were used. results: this study evaluated 903 children and adolescents (51.7% boys) whose mean age was 9.3±2.5 years. the prevalence of pre-hypertension and hypertension was 9.1% and 2.9%. there was a positive correlation between both systolic and diastolic blood pressure and anthropometric variables, especially for weight (r=0.53 and r=0.45, p<0.05) and waist circumference (r=0.50 and r=0.38, p<0.05). conclusions: the prevalence of elevated blood pressure in this study was similar to what has been reported in international and national studies. a positive correlation with abnormal anthropometric measures was found. these results suggest that overweight affects blood pressure already in childhood.
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