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Validation of Analytical Method for Chromatographic Determination of Chlorimuron Ethyl Herbicide in Sediments  [PDF]
Ramom Rachide Nunes, Bruno Henrique Froes Santos, Maria Olímpia Oliveira Rezende
Open Access Library PrePrints (OALib PrePrints) , 2014, DOI: 10.4236/oalib.preprints.1200090
Abstract: The present work describes the validation of an analytical method for chromatographic determination of chlorimuron ethyl herbicide (compound of sulphonylurea group) in sediments, in according with Brazilian legislation, following the guidance and normative documents of INMETRO (DOQ-CGCRE-008) and ANVISA (Resolution RE no 899 - 2003). The results show that the chromatographic method demonstrated to be selective, accurate, precision and linear over the concentration range of 5.0-1000.0 ug L-1. Besides, limits of quantitation and determination were satisfactory, 29.48 and 8.84 ug L-1, both determined by calibration curve. The method proposed also showed good results for assays of recovery, with confidence intervals between 80 and 100%, approximately, with accuracy and precision.
Analytical Procedures and Method Validation for Determination of Chlorimuron Ethyl in Sediments  [PDF]
Ramom Rachide Nunes, Bruno Henrique Froes Santos, Maria Olimpia de Oliveira Rezende
Open Access Library Journal (OALib Journal) , 2016, DOI: 10.4236/oalib.1102398
Abstract: The present work describes the validation of an analytical method for chromatographic determination of chlorimuron ethyl herbicide (compound of sulphonylurea group) in sediments, in according with Brazilian legislation, following the guidance and normative documents of INMETRO (DOQ-CGCRE-008) and ANVISA (Resolution RE no. 899-2003). The results show that the chromatographic method demonstrated to be selective, accurate, precision and linear over the concentration range of 5.0 - 1000.0 μg·L﹣1. Besides, limits of quantitation and determination were satisfactory, 29.48 and 8.84 μg·L﹣1, both determined by calibration curve. The method proposed also showed good results for assays of recovery, with confidence intervals between 80 and 100%, approximately, with accuracy and precision.
Toxicity of herbicides on Escherichia coli growth
Botelho, RG;Froes, CM;Santos, JB;
Brazilian Journal of Biology , 2012, DOI: 10.1590/S1519-69842012000100016
Abstract: agriculture uses a huge variety and quantity of chemicals. if, on one hand, the goal is to increase productivity, on the other hand these products contaminate aquatic environments. among these products, herbicides deserve greater attention in relation to contamination of aquatic environments due to their extensive use to weed control. this study was carried out because the effects of these molecules on aquatic microorganisms such as escherichia coli, is still unclear. using microdilution plate assays, escherichia coli were exposed to various commercial formulations of herbicides widely used in brazil. the herbicide paraquat was the only one able to prevent the growth of escherichia coli and is characterized as bacteriostatic.
Pathogenetic trial of boric acid in bean and tomato plants
Solange Gomes Carneiro,Euclides Davidson Bueno Romano,Tiago Henrique dos Santos Garbim,Bruno Garcia de Oliveira
International Journal of High Dilution Research , 2011,
Abstract: Background: homeopathy is held in organic agriculture as a means to control disease and plagues. However, different from doctors, who have works on materia medica and repertories available to choose the most suitable homeopathic medicine for each patient, agronomists do not yet have an equivalent Homeopathic Materia Medica of Plants (HMMP) describing symptoms observed in plants. Aim: the aim of this study was to carry out a homeopathic pathogenetic trial (HPT) in plants comparing the effects elicited by boric acid in ponderable dose and dilution 6cH in two different plant species, namely bean and tomato cultivars. Methods: 4 tests were carried out, 2 on tomato and 2 on bean plants, which received 1 to 6 applications of treatments. Results: there were differences between both species regarding their sensitiveness to boric acid. None of the tomato plants that received Boron 6cH showed symptoms, differently from bean plants. On the other hand, in tests of ponderable doses of boric acid, tomato plants exhibited 3 symptoms more than bean plants. A higher number of bean plants exhibited symptoms with boric acid in ponderable dose than in dilution 6cH). Nos ensaios com feijoeiro, um maior n omero de plantas apresentou sintomas ap 3s o tratamento com cido b 3rico na dose ponderal do que com cido b 3rico 6cH. Conclusions: these results suggest that the elaboration of a HMMP must take into account the species in which symptoms were obtained. Moreover, HPTs in plants must be carried out with both ponderable doses and high dilutions in order for differences in sensitiveness among species be better identified.
Influence of environmental factors on the concentration of phenolic compounds in leaves of Lafoensia pacari
Bruno Leite Sampaio,Maria Teresa F. Bara,Pedro Henrique Ferri,Suzana da Costa Santos
Revista Brasileira de Farmacognosia , 2011,
Abstract: Lafoensia pacari A. St.-Hil., Lythraceae, a plant from the Cerrado known as pacari or dedaleiro, is widely used as an antipyretic, wound healing, anti-inflammatory, antidiarrheal and in the treatment of gastritis and cancer. Notable among the metabolite groups identified in leaves of L. pacari are the polyphenols, such as tannins and flavonoids, related to the pharmacological activities of pacari. Studies on the influence of environmental factors over production of major groups of secondary metabolites in pacari are important because they contribute data for its cultivation and harvest, and establish quantitative parameters of secondary metabolites in the plant drug. The objective of this study was to evaluate the influence of environmental factors on concentrations of phenolic metabolites in the leaves of L. pacari. Compounds quantified in the leaves were: total phenols, tannins by protein precipitation, hydrolysable tannins, total flavonoids, ellagic acid and mineral nutrients, while soil fertility was also analyzed, all over a period of one year. The data were analyzed using multivariate analysis, and the results suggest that metabolite concentrations in the leaves of this plant are influenced by seasonal factors, in particular the temperature and foliar micronutrients (Cu, Fe, Mn, Zn).
Efeito de diferentes laminas de irriga??o na produ??o de óleo essencial do manjeric?o (Ocimum basilicum L.)
Pravuschi, Paulo Roberto;Marques, Patricia Angélica Alves;Rigolin, Bruno Henrique Marega;Santos, Ana Cláudia Pacheco;
Acta Scientiarum. Agronomy , 2010, DOI: 10.4025/actasciagron.v32i4.3160
Abstract: basil (ocimum basilicum l.) is an aromatic plant of economic importance in brazil, very used as a spice for food preparation. it is also used to obtain essential oil, which important in the perfume industry. the purpose of this experiment was to analyze the effect of five irrigation water depths, as well as an economic viability analysis of the application of irrigation in basil production. the experiment was carried out from march to october 2007. the water depths were based in the cumulative class a pan evaporation (cpe): 0, 50, 75, 100 and 150% cpe. the analyzed variables were dry weight for leaves and flowers, content of essential oil, and dry mass and length of root zone. the essential oil yield and net return were calculated. the third harvest was possible only under irrigation. the daily excessive water depth was detrimental to plant development. the 100% cpe water depth showed the maximum dry mass productivity (4,248.67 kg ha-1), as well as the maximum essential oil yield (58.26 kg ha-1) with net annual profit of r$ 2,543.82 ha-1.
Caracteriza??o dos medicamentos prescritos aos idosos na Estratégia Saúde da Família
Oliveira, Camila Alves Paes de;Marin, Maria José Sanches;Marchioli, Milton;Pizoletto, Bruno Henrique Magalh?es;Santos, Rafael Varella dos;
Cadernos de Saúde Pública , 2009, DOI: 10.1590/S0102-311X2009000500007
Abstract: considering that the elderly are heavy consumers of medications, with significant consequences for their living conditions, the current study was intended to analyze the characteristics of such drug prescription, using the indicators proposed by the world health organization (who), and to characterize the drug classes prescribed to them in four units under the family health strategy (fhs) in marília, s?o paulo state, brazil. one family health unit (fhu) was selected by region of the city, and 100 patient charts were checked in fhu-a; 86 in b, 99 in c, and 97 in d. according to who drug prescription criteria, the results were similar to the recommended patterns except for drug prescription by generic name. in addition, there were significant differences between the family health units. the most frequently prescribed drug classes were for cardiovascular disorders. the health professionals showed a concern for adjusting drug prescription to rational-use criteria. however, there is a need for understanding the differences in prescription between units and a search for consensus, aimed at rational drug use.
Influence of environmental factors on the concentration of phenolic compounds in leaves of Lafoensia pacari
Sampaio, Bruno Leite;Bara, Maria Teresa F.;Ferri, Pedro Henrique;Santos, Suzana da Costa;Paula, José Realino de;
Revista Brasileira de Farmacognosia , 2011, DOI: 10.1590/S0102-695X2011005000177
Abstract: lafoensia pacari a. st.-hil., lythraceae, a plant from the cerrado known as pacari or dedaleiro, is widely used as an antipyretic, wound healing, anti-inflammatory, antidiarrheal and in the treatment of gastritis and cancer. notable among the metabolite groups identified in leaves of l. pacari are the polyphenols, such as tannins and flavonoids, related to the pharmacological activities of pacari. studies on the influence of environmental factors over production of major groups of secondary metabolites in pacari are important because they contribute data for its cultivation and harvest, and establish quantitative parameters of secondary metabolites in the plant drug. the objective of this study was to evaluate the influence of environmental factors on concentrations of phenolic metabolites in the leaves of l. pacari. compounds quantified in the leaves were: total phenols, tannins by protein precipitation, hydrolysable tannins, total flavonoids, ellagic acid and mineral nutrients, while soil fertility was also analyzed, all over a period of one year. the data were analyzed using multivariate analysis, and the results suggest that metabolite concentrations in the leaves of this plant are influenced by seasonal factors, in particular the temperature and foliar micronutrients (cu, fe, mn, zn).
Susceptibility of the predator Euborellia annulipes (Dermaptera: Anisolabididae) to mycoinsecticides
QUEIROZ DE OLIVEIRA,FLáVIA; DE LUNA BATISTA,JACINTO; BRUNO MALAQUIAS,JOSé; HENRIQUE DE BRITO,CARLOS; PEREIRA DOS SANTOS,EMMERSON;
Revista Colombiana de Entomología , 2011,
Abstract: to evaluate the infuence of beauveria bassiana (balsamo) vuill and metarhizium anisopliae (metsch) sorok on the survival of the predator euborellia annulipes lucas (dermaptera: anisolabididae) were used product concentrations 5.00x109; 7.50x109; 10.00x109; 12.50x109 conidia/l from isolates of b. bassiana and m. anisopliae which were obtained, respectively, through products boveril? and metarril? and control (0.0 conidia/l). high rates of hatching nymphs of e. annulipes have been recorded in clutches that received applications of m. anisopliae. moreover, b. bassiana has affected negatively the hatching rate of nymphs of e. annulipes. a slightly harmful effect has been observed in all concentrations of conidia b. bassiana in clutches of e. annulipes. the survival was 100% in all tested concentrations of m. anisopliae in females of e. annulipes. the females which received topical application of b. bassiana have shown a survival rate ranging from 80.30 (12.50x109 conidia) to 100% (5.00x109 conidia). in males of e. annulipes, the survival rate in insects treated with b. bassiana ranged from 95.00 (12.50x109 conidia) to 100% (5.00x109 conidia), while with m. anisopliae applications this rate was 96.02 (12.50x109 conidia) to 100% (5.00x109 conidia). the entomopathogenic fungi b. bassiana and m. anisopliae did not affect the mortality of nymphs and adults of e. annulipes. however, careful measures should be adopted in applications of b. bassiana directly into clutches of e. annulipes.
Efeito de diferentes laminas de irriga o na produ o de óleo essencial do manjeric o (Ocimum basilicum L.) = Effect of different water depths in basil (Ocimum basilicum L.) oil production
Paulo Roberto Pravuschi,Patricia Angélica Alves Marques,Bruno Henrique Marega Rigolin,Ana Cláudia Pacheco Santos
Acta Scientiarum : Agronomy , 2010,
Abstract: O manjeric o (Ocimum basilicum L.) é uma planta aromática de importancia econ mica no Brasil, muito usado como tempero na prepara o de alimentos. é também utilizado para a obten o de óleo essencial, importante na indústria de perfumaria. O objetivo deste experimento foi analisar o efeito de cinco laminas de irriga o, bem como uma análise da viabilidade econ mica da aplica o da irriga o na produ o do manjeric o. O experimento foi conduzido no período de mar o a outubro de 2007. As laminas foram baseadas na evapora o do Tanque Classe A (ECA) sendo: 0, 50, 75, 100 e 150% ECA. As variáveis analisadas foram matéria seca das folhas e inflorescências, teor de óleo essencial, massa seca e comprimento de raízes. Foram calculadas a produtividade de óleo essencial e a receita líquida. A terceira colheita foi viável somente utilizando irriga o. A irriga o diáriaexcessiva prejudicou o desenvolvimento da planta. A lamina de irriga o equivalente a 100% ECA promoveu a máxima produtividade de matéria seca (4.248,67 kg ha-1), assim como a máxima produtividade de óleo essencial (58,26 kg ha-1), apresentando receita líquida anualde R$ 2.543,82 ha-1. Basil (Ocimum basilicum L.) is an aromatic plant of economic importance in Brazil, very used as a spice for food preparation. It is also used to obtain essential oil, which important in the perfume industry. The purpose of this experiment was to analyze the effect of five irrigation water depths, as well as an economic viability analysis of the application of irrigation in basil production. The experiment was carried out from March to October 2007. The water depths were based in the cumulative class A pan evaporation (CPE): 0, 50, 75, 100 and 150% CPE. The analyzed variables were dry weight for leaves and flowers, content of essential oil, and dry mass and length of root zone. The essential oil yield and net return were calculated. The third harvest was possible only under irrigation. The daily excessive water depth was detrimental to plant development. The 100% CPE water depth showed the maximum dry mass productivity (4,248.67 kg ha-1), as well as the maximum essential oil yield (58.26 kg ha-1) with net annual profit of R$ 2,543.82 ha-1.
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