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Search Results: 1 - 10 of 20965 matches for " Bok-Kyu Kim "
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Antiasthmatic Effects of Hesperidin, a Potential Th2 Cytokine Antagonist, in a Mouse Model of Allergic Asthma
Seung-Hyung Kim,Bok-Kyu Kim,Young-Cheol Lee
Mediators of Inflammation , 2011, DOI: 10.1155/2011/485402
Abstract: Background and Objective. The features of asthma are airway inflammation, reversible airflow obstruction, and an increased sensitivity to bronchoconstricting agents, termed airway hyperresponsiveness (AHR), excess production of Th2 cytokines, and eosinophil accumulation in the lungs. To investigate the antiasthmatic potential of hesperidin as well as the underlying mechanism involved, we studied the inhibitory effect and anti-inflammatory effect of hesperidin (HPN) on the production of Th2 cytokines, eotaxin, IL-17, -OVA-specific IgE in vivo asthma model mice. Methods. In this paper, BALB/c mice were systemically sensitized to ovalbumin (OVA) followed intratracheally, intraperitoneally, and by aerosol allergen challenges. We investigated the effect of HPN on airway hyperresponsiveness, pulmonary eosinophilic infiltration, various immune cell phenotypes, Th2 cytokine production and OVA-specific IgE production in a mouse model of asthma. Results. In BALB/c mice, we found that HPN-treated groups had suppressed eosinophil infiltration, allergic airway inflammation, and AHR, and these occurred by suppressing the production of IL-5, IL-17, and OVA-specific IgE. Conclusions. Our data suggest that the therapeutic mechanism by which HPN effectively treats asthma is based on reductions of Th2 cytokines (IL-5), eotaxin, OVA-specific IgE production, and eosinophil infiltration via inhibition of GATA-3 transcription factor. 1. Introduction The features of asthma are airway inflammation, reversible airflow obstruction, and an increased sensitivity to bronchoconstricting agents, termed airway hyperresponsiveness (AHR), excess production of Th2 cytokines, and eosinophil accumulation in the lungs [1]. The immune cells involved in regulating allergic airway inflammatory responses include monocytes/macrophages, dendritic cells, neutrophils, basophils, mast cells, eosinophils, T and B lymphocytes [2]. Moreover, it is well established that there is a strong interaction between eosinophils and Th2 cells in the asthmatic airways and that Th2 cell-derived cytokines, namely IL-4, IL-5, and IL-13, play critical roles in orchestrating and amplifying allergic inflammation in asthma [3]. CD4+ T cells play a crucial role in immune protection, and they do so through their capacity to help B cells make antibodies, to recruit neutrophils, eosinophils, and basophils to sites of inflammation, and, through their production of cytokines and chemokines, to orchestrate the immune responses [4]. Suppression of cytokine production in activated CD4+ T cells may be useful for the treatment of
Effects of Corni fructus on ovalbumin-induced airway inflammation and airway hyper-responsiveness in a mouse model of allergic asthma
Seung-Hyung Kim, Bok-Kyu Kim, Young-Cheol Lee
Journal of Inflammation , 2012, DOI: 10.1186/1476-9255-9-9
Abstract: In this study, BALB/c mice were systemically sensitized to ovalbumin (OVA) by intraperitoneal (i.p.), intratracheal (i.t.) injections and intranasal (i.n.) inhalation of OVA. We investigated the effect of CF on airway hyperresponsiveness, pulmonary eosinophilic infiltration, various immune cell phenotypes, Th2 cytokine production, and OVA-specific immunoglobulin E (IgE) production.The CF-treated groups showed suppressed eosinophil infiltration, allergic airway inflammation, and AHR via reduced production of interleuin (IL) -5, IL-13, and OVA-specific IgE.Our data suggest that the therapeutic effects of CF in asthma are mediated by reduced production of Th2 cytokines (IL-5), eotaxin, and OVA-specific IgE and reduced eosinophil infiltration.Allergic asthma generally presents with symptoms of wheezing, coughing, breathlessness, and airway inflammation. It is a chronic inflammatory disease of the airways, characterized by airway eosinophilia and goblet cell hyperplasia with mucus hypersecretion to inhaled allergens and nonspecific stimuli [1,2]. In particular, eosinophilic inflammation is considered the hallmark of airway inflammation in asthma [3]. The inflammatory process in allergic asthma is dominated by Th2 cells that produce IL-4, IL-5, and IL-13 [4], which activate eosinophils and induce the production of IgE by B cells [5,6].CF is a Korean traditional medicinal herb with tonic, analgesic, and diuretic activity and has been commonly used to facilitate liver and kidney function, reduce urination, and decrease perspiration. We previously reported that CF has anti-allergic activity in mouse splenic B cells and IC-2 mast cells [7]. Sung et al. [8] reported that aqueous extracts of CF have anti-inflammatory and analgesic activities in murine RAW 264.7 macrophage cells. Moreover, Du et al. [9] reported that the polysaccharides in crude and processed CF enhance nonspecific immunity, specific humoral immunity, and specific cellular immunity in immunosuppressed mice, and
Antiasthmatic Effects of Herbal Complex MA and Its Fermented Product MA128
Dong-Seon Kim,Seung-Hyung Kim,Bok-Kyu Kim,Min Cheol Yang,Jin Yeul Ma
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/769508
Abstract: This study was conducted to determine if oral administration of the novel herbal medicine, MA, and its Lactobacillus acidophilus fermented product, MA128, have therapeutic properties for the treatment of asthma. Asthma was induced in BALB/c mice by systemic sensitization to ovalbumin (OVA) followed by intratracheal, intraperitoneal, and aerosol allergen challenges. MA and MA128 were orally administered 6 times a week for 4 weeks. At 1 day after the last ovalbumin exposure, airway hyperresponsiveness was assessed and samples of bronchoalveolar lavage fluid, lung cells, and serum were collected for further analysis. We investigated the effect of MA and MA128 on airway hyperresponsiveness, pulmonary eosinophilic infiltration, various immune cell phenotypes, Th2 cytokine production, OVA-specific IgE production, and Th1/Th2 cytokine production in this mouse model of asthma. In BALB/c mice, we found that MA and MA128 treatment suppressed eosinophil infiltration into airways and blood, allergic airway inflammation and AHR by suppressing the production of IL-5, IL-13, IL-17, Eotaxin, and OVA-specific IgE, by upregulating the production of OVA-specific Th1 cytokine (IFN-γ), and by downregulating OVA-specific Th2 cytokine (IL-4) in the culture supernatant of spleen cells. The effectiveness of MA was increased by fermentation with Lactobacillus acidophilus.
Pinellia ternata, Citrus reticulata, and Their Combinational Prescription Inhibit Eosinophil Infiltration and Airway Hyperresponsiveness by Suppressing CCR3+ and Th2 Cytokines Production in the Ovalbumin-Induced Asthma Model
In-Soo Ok,Seung-Hyung Kim,Bok-Kyu Kim,Jang-Cheon Lee,Young-Cheol Lee
Mediators of Inflammation , 2009, DOI: 10.1155/2009/413270
Abstract: Background and Objective. This study was aimed to analyse the curative effects of Pinellia ternata, Citrus reticulata, and their combination on airway hyperresponsiveness (AHR) to inhaled methacholine, pulmonary eosinophilic infiltration, Th2 cytokine production, and IgE and histamine production in a murine model of asthma. Methods. For this purpose, BALB/c mice were systemically sensitized to ovalbumin (OVA) followed intratracheally, intraperitoneally, and by aerosol allergen challenges for 12 weeks. We examined the development of pulmonary eosinophilic accumulation, control of Th2 cytokine, immunoglobulin E (IgE), and histamine productions in a murine model of asthma. Results. Our data suggest that the therapeutic mechanism by which Pinellia ternata, Citrus reticulata, and their combinational prescription effectively treats asthma is based on reductions of eosinophil infiltration, eotaxin receptor (CCR3), histamine, OVA-specific IgE productions in serum, and Th2 cytokines (IL-5, IL-13) by marked reductions of IL-5 and IL-13 mRNA expression in lung tissue. Conclusions. These findings provide evidence that Pinellia ternata, Citrus reticulata, and their combination play a regulatory role in allergic inflammation and offer therapeutic approaches as novel CCR3 antagonists for treatment asthma. However, it is not clear whether pharmacological activities of prescription composed of two herbs are potentiated due to synergistic effect or additive effect.
Protein interaction network related to Helicobacter pylori infection response
Kyu Kwang Kim, Han Bok Kim
World Journal of Gastroenterology , 2009,
Abstract: AIM: To understand the complex reaction of gastric inflammation induced by Helicobacter pylori (H pylori) in a systematic manner using a protein interaction network.METHODS: The expression of genes significantly changed on microarray during H pylori infection was scanned from the web literary database and translated into proteins. A network of protein interactions was constructed by searching the primary interactions of selected proteins. The constructed network was mathematically analyzed and its biological function was examined. In addition, the nodes on the network were checked to determine if they had any further functional importance or relation to other proteins by extending them.RESULTS: The scale-free network showing the relationship between inflammation and carcinogenesis was constructed. Mathematical analysis showed hub and bottleneck proteins, and these proteins were mostly related to immune response. The network contained pathways and proteins related to H pylori infection, such as the JAK-STAT pathway triggered by interleukins. Activation of nuclear factor (NF)-κB, TLR4, and other proteins known to function as core proteins of immune response were also found. These immune-related proteins interacted on the network with pathways and proteins related to the cell cycle, cell maintenance and proliferation, and transcription regulators such as BRCA1, FOS, REL, and zinc finger proteins. The extension of nodes showed interactions of the immune proteins with cancer-related proteins. One extended network, the core network, a summarized form of the extended network, and cell pathway model were constructed.CONCLUSION: Immune-related proteins activated by H pylori infection interact with proto-oncogene proteins. The hub and bottleneck proteins are potential drug targets for gastric inflammation and cancer.
Expression of P2 receptors in human B cells and Epstein-Barr virus-transformed lymphoblastoid cell lines
Dong Hyeon Lee, Kyu Sang Park, In Deok Kong, Jun Woo Kim, Bok Ghee Han
BMC Immunology , 2006, DOI: 10.1186/1471-2172-7-22
Abstract: The mRNA transcripts of most P2 receptors were detected in primary B cells; the expression of P2X3 and P2X7 receptors was the lowest of all the P2 receptors. By contrast, LCLs expressed several dominant P2 receptors – P2X4, P2X5, and P2Y11 – in amounts similar to those seen in B cells infected with EBV for 2 weeks. The amount of most P2 subtypes in LCLs or EBV-infected B cells was lower than in normal B cells. However, the amount of P2X7 receptor expressed in LCLs was higher. Protein expression was studied using Western blotting to confirm the mRNA findings for P2X1, P2X4, P2X7, P2Y1, and P2Y11 receptors. ATP increased the intracellular free Ca2+ concentration ([Ca2+]i) by enhancing the Ca2+ influx in both B cells and LCLs in a dose-dependent manner.These findings describe P2 receptor expression profiles and the effects of purinergic stimuli on B cells and suggest some plasticity in the expression of the P2 receptor phenotype. This may help explain the nature and effect of P2 receptors on B cells and their role in altering the characteristics of LCLs.B cells synthesize and secrete large quantities of soluble immunoglobulin antibodies and thus, play a key role in humoral immunity. An infection with the Epstein-Barr virus (EBV) easily transforms resting primary B cells in vitro from human peripheral blood cells into B-blast-like proliferating lymphoblastoid cell lines (LCLs) [1]. This infection is used routinely in the laboratory to generate LCLs from B cells [2]. LCLs are widely used in various types of studies, including those involving the disciplines of immunology, cellular biology, and genetics. This transformation results in changes in certain cellular properties, including gene expression [3], cell surface phenotyping, and cytokine production [4].Extracellular nucleotides – e.g., adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate, uracil 5'-triphosphate, and uracil 5'-diphosphate – have various physiological effects in many cells, such as exocrine and en
Chemical characterization of rainwater over Suwon region during farming and non-farming periods  [PDF]
Min-Kyeong Kim, Jong-Sik Lee, Goo-Bok Jung, Soon-Ik Kwon, Byong-Gu Ko, Min-Young Kim, Kyu-Ho So, Sun-Gang Yun
Journal of Agricultural Chemistry and Environment (JACEN) , 2013, DOI: 10.4236/jacen.2013.21001
Abstract:

Recently, special attention has been givento acid rain and its problem to environment such as acid precipitation and air pollution in East Asia.In the present study, rainwater samples werecollected from Apr to Oct (farming period) and from Nov to Mar (non-farming period) in 2009 ~ 2011. The samples were chemically characterized for the assessment of emission sources. Suwon region, a typical agricultural area in Gyeonggi province (South Korea) was chosen as a study site. The content of ionic species and theirtemporal variation were used forfactor analysis, which was used to presume the natural and anthropogenic sources depending upon the farming and non-farming periods. The cationsobserved during farming and non-farming periods were > Na+> Ca2+> K+> H+> Mg2+and Na+> N >Ca2+> H+= Mg2+> K+, respectively. The anions during farming and non-farming periods were > > Cl- and > > Cl-, respectively. While the amounts of sulfate, one of the major dissolved components of rainwater were 113.5 and 177.0 ueq.L-1, the ones of NSS- (Non-Sea Salt sulfate) were 93.7 and 87.1% during farming and non-farming periods, respectively. The comparison of observed pH values (pHobs) with the theoretical pH values (pHthe) showed that the neutralization of rainwater considerably went along during farming and non-farming periods. The highest amountof rainfall throughout the year was310.5 mmin August and its corre sponding nitrogen loading was5.03 kg·ha-1. The major ion contents for crop growth, \"\", Ca2+, K+, Mg2+, were 49.4, 5.5, 18.1,1.4 kg·ha-1from April to October. The major contributions to the existing chemical ions in rainwaters during farming and non-farming periods were from the natural sources (sea and soil) and the anthropogenic sources (human and animal waste, vehicular emission + fossil fuels combustion), respectively.


Heavy metal chemistry in soils received long-term application of organic wastes  [PDF]
Soon-Ik Kwon, Yeon-Ah Jang, Kye-Hoon Kim, Goo-Bok Jung, Min-Kyeong Kim, Hae Hwang, Mi-Jin Chae, Seung-Chang Hong, Kyu-Ho So, Sun-Gang Yun, Kwon-Rae Kim
Journal of Agricultural Chemistry and Environment (JACEN) , 2012, DOI: 10.4236/jacen.2012.11001
Abstract: This study was carried out to understand the long-term effect of organic waste treatment on the fate of heavy metals originated from the organic wastes, together with examination of changes in soil properties. For this, the soils received three different organic wastes (municipal sewage sludge, alcohol fermentation processing sludge, pig manure compost) in three different rates (12.5, 25, 50 ton/ha/yr) for 7 years (1994 - 2000) were used. To see the long-term effect, plant growth study and soil examination were conducted twice in 2000 and 2010, respectively. There was no additional treatment of organic wastes for post ten years after ceasing organic waste treatment for seven years. Soil examination conducted in 2010 showed decreases in soil pH, EC, total nitrogen, organic matter, available phosphorus, exchangeable cations and heavy metal contents in all soils received organic wastes compared to the results obtained in 2000. Speciation of heavy metals in soil through sequential extraction showed that organically bound Cu was the dominant species in all treatment and exchangeable Cu was increased in the plots treated with municipal sewage sludge and alcohol fermentation processing sludge. organically bound Ni increased from 25% - 30% to 32% - 45% in 2010 inall treatment while Pb showed increase in carbonate form in all treatments. Zn existed mainly as sulfide and residual forms, showing increases in organically bound form in all treatment during post ten years.


Using Process Indicators to Facilitate Data-Driven Decision Making in the Era of Accountability  [PDF]
Kyu Tae Kim
Creative Education (CE) , 2012, DOI: 10.4236/ce.2012.36102
Abstract: This paper explores which accountability indicators are likely to reveal the distinct contexts and qualitative characteristics of school that stimulate and improve authentic pedagogy and accountability. In the era of accountability, data-driven decision making is a new research area for authentic pedagogy through monitoring student progress and improving school accountability. It is based on input-and-result oriented indicators such as school demographics, facilities, budget, standardized test scores, dropout rates. But the indicators are unlikely to capture a dynamically interactive qualitative characteristics of school organizations featuring a loosely-coupled system and difficult to be measured or assessed. Thus, process indicators need to be complementary to input-and-outcome data for a valid and graphic description, monitoring and explanation of ‘why’ and ‘how’ the school outcomes occur. The author concluded that the data-driven decision making (DDDM) based on process indicators strengthens reflective professionalism and provides for the educational welfare for the poor and left-behind students.
An Assessment of Survival among Korean Elderly Patients Initiating Dialysis: A National Population-Based Study
Shina Lee, Jung-Hwa Ryu, Hyunwook Kim, Kyoung Hoon Kim, Hyeong Sik Ahn, Hoo Jae Hann, Yongjae Cho, Young Mi Park, Seung-Jung Kim, Duk-Hee Kang, Kyu Bok Choi, Dong-Ryeol Ryu
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0086776
Abstract: Background Although the proportion of the elderly patients with incident end-stage renal disease (ESRD) patients has been increasing in Korea, there has been a lack of information on outcomes of dialysis treatment. This study aimed to assess the survival rate and to elucidate predictors for all-cause mortality among elderly Korean patients initiating dialysis. Methods We analyzed 11,301 patients (6,138 men) aged 65 years or older who had initiated dialysis from 2005 to 2008 and had followed up (median, 37.8 months; range, 3–84 months). Baseline demographics, comorbidities and mortality data were obtained using the database from the Health Insurance Review & Assessment Service. Results The unadjusted 5-year survival rate was 37.6% for all elderly dialysis patients, and the rate decreased with increasing age categories; 45.9% (65~69), 37.5% (70~74), 28.4% (75~79), 24.1% (80~84), and 13.7% (≥85 years). The multivariate Cox proportional hazard model revealed that age, sex, dialysis modality, the type of insurance, and comorbidities such as diabetes mellitus, myocardial infarction, congestive heart failure, peripheral vascular disease, cerebrovascular disease, dementia, chronic pulmonary disease, hemiparesis, liver disease, and any malignancy were independent predictors for mortality. In addition, survival rate was significantly higher in patients on hemodialysis compared to patients on peritoneal dialysis during the whole follow-up period in the intention-to-treat analysis. Conclusions Survival rate was significantly associated with age, sex, and various comorbidities in Korean elderly patients initiating dialysis. The results of our study can help to provide relevant guidance on the individualization strategy in elderly ESRD patients requiring dialysis.
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