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A Sensitive Quantification of HHV-6B by Real-time PCR
ster Bodil,H?llsberg Per
Biological Procedures Online , 2002, DOI: 10.1251/bpo38
Abstract: Human herpesvirus (HHV)-6B is a pathogen causing latent infection in virtually all humans. Nevertheless, the interaction of HHV-6B with its host cells is poorly understood. Although HHV-6B is approximately 90% homologous to HHV-6A, it expresses certain B-specific genes. In order to quantify the amount of expressed viral mRNA we have developed a method using real-time PCR on a LightCycler instrument. Here we describe an assay for the detection of the HHV-6B B6 mRNA, but our approach can easily be extended to involve other mRNAs. This method is useful during the study of HHV-6B biology and offers reliable and reproducible, quantitative detection of viral mRNA below the attomol range.
Keratin23 (KRT23) Knockdown Decreases Proliferation and Affects the DNA Damage Response of Colon Cancer Cells
Karin Birkenkamp-Demtr?der, Stephan A. Hahn, Francisco Mansilla, Kasper Thorsen, Abdelouahid Maghnouj, Rikke Christensen, Bodilster, Torben Falck ?rntoft
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0073593
Abstract: Keratin 23 (KRT23) is strongly expressed in colon adenocarcinomas but absent in normal colon mucosa. Array based methylation profiling of 40 colon samples showed that the promoter of KRT23 was methylated in normal colon mucosa, while hypomethylated in most adenocarcinomas. Promoter methylation correlated with absent expression, while increased KRT23 expression in tumor samples correlated with promoter hypomethylation, as confirmed by bisulfite sequencing. Demethylation induced KRT23 expression in vitro. Expression profiling of shRNA mediated stable KRT23 knockdown in colon cancer cell lines showed that KRT23 depletion affected molecules of the cell cycle and DNA replication, recombination and repair. In vitro analyses confirmed that KRT23 depletion significantly decreased the cellular proliferation of SW948 and LS1034 cells and markedly decreased the expression of genes involved in DNA damage response, mainly molecules of the double strand break repair homologous recombination pathway. KRT23 knockdown decreased the transcript and protein expression of key molecules as e.g. MRE11A, E2F1, RAD51 and BRCA1. Knockdown of KRT23 rendered colon cancer cells more sensitive to irradiation and reduced proliferation of the KRT23 depleted cells compared to irradiated control cells.
Inhibition of p53-Dependent, but Not p53-Independent, Cell Death by U19 Protein from Human Herpesvirus 6B
Emil Kofod-Olsen, Janni M. L. M?ller, Mariane H. Schleimann, Bettina Bundgaard, Rasmus O. Bak, Bodilster, Jacob G. Mikkelsen, Ted Hupp, Per H?llsberg
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0059223
Abstract: Infection with human herpesvirus (HHV)-6B alters cell cycle progression and stabilizes tumor suppressor protein p53. In this study, we have analyzed the activity of p53 after stimulation with p53-dependent and -independent DNA damaging agents during HHV-6B infection. Microarray analysis, Western blotting and confocal microscopy demonstrated that HHV-6B-infected cells were resistant to p53-dependent arrest and cell death after γ irradiation in both permissive and non-permissive cell lines. In contrast, HHV-6B-infected cells died normally through p53-independet DNA damage induced by UV radiation. Moreover, we identified a viral protein involved in inhibition of p53 during HHV-6B-infection. The protein product from the U19 ORF was able to inhibit p53-dependent signaling following γ irradiation in a manner similar to that observed during infection. Similar to HHV-6B infection, overexpression of U19 failed to rescue the cells from p53-independent death induced by UV radiation. Hence, infection with HHV-6B specifically blocks DNA damage-induced cell death associated with p53 without inhibiting the p53-independent cell death response. This block in p53 function can in part be ascribed to the activities of the viral U19 protein.
Doing Care with Integrity and Emotional Sensibility—Reciprocal Encounters in Psychiatric Community Care of Older People with Mental Health Problems  [PDF]
Lis Bodil Karlsson, Elisabeth Rydwik
Sociology Mind (SM) , 2013, DOI: 10.4236/sm.2013.32025

The article focuses on the experiences of community care workers in the encounter with older persons suffering from mental health problems, such as mental illness and disability. The purpose is to describe and discuss opportunities for and challenges to reciprocal encounters with these older people in community care, based on statements from professionals interviewed. Structured conversations with five focus groups were organised, consisting of 26 participants, including nurses’ assistants, assistant nurses, nurses, social workers and occupational therapists. The participants in the focus groups highlight the essence of being involved and create space for a reflective attitude. Clinical implications will be presented as well.

An International Trade Model with Entrepreneurs and Financial Markets  [PDF]
Bodil O. Hansen, Hans Keiding
Technology and Investment (TI) , 2016, DOI: 10.4236/ti.2016.73012
We consider a two-period model of a three-country world with free trade in finished products and some factor mobility, where production is subject to uncertainty. Enterpreneurs may establish production in other countries but can obtain financing only in the country of origin. In this model, integrating production across countries, in particular integrating economically strong and weak partners, may give rise to a welfare loss, showing that traditional views on efficiency of international trade must be reconsidered when risk and uncertainty are taken into account.
A case report on a patient suffering from recurrent vomiting episodes, whose condition improved markedly during pregnancy and breast feeding
Bodil Ohlsson
BMC Gastroenterology , 2006, DOI: 10.1186/1471-230x-6-28
Abstract: A woman with recurrent vomiting episodes over several years was examined by esophagogastroduodenoscopy. This showed a non-peristaltic ventricle. Treatment with the procinetic drug cisapride (Prepulsid?) improved the peristalsis and reduced the symptoms. During pregnancy and breast feeding, she was free of symptoms, in spite of having discontinued her medication with cisapride (Prepulsid?).The fact that the patient improved during pregnancy and breast feeding, would seem to indicate the involvement of factors in the physiology of pregnancy and breast feeding that are of importance for gastric motility. This deserves further investigation.The normal motor function of the gut is controlled by the sympathetic and parasympathetic nervous system in addition to the enteric nervous system, interstitial cells of Cajal (ICCs) and smooth muscle cells. Severe gastrointestinal dysmotility, including gastroparesis, can develop as a result of the abnormal function of any of these systems including their associated neurotransmitters [1]. The pathogenesis may be immune-mediated, as in post-infectious gastroparesis [2], or may be due to defects in the ICCs, autonomic nervous system and smooth muscle, as in diabetic gastroparesis [3]. One third of all cases of gastroparesis are still regarded as idiopathic [4]. Several hormones and neurotransmitters have been suggested to be involved in this condition [5]. In clinical practice, I have noticed that some patients with severe gastrointestinal motility problems have improved during pregnancy and breast feeding.In the following, I describe a woman with recurrent vomiting and reduced gastric motility, who was completely free of problems during pregnancy and breast feeding. A hypothesis could be raised on this finding, namely, that peptides and/or neurotransmitters appearing in altered concentrations during these conditions are involved in the physiology and pathophysiology of the gastrointestinal tract.A previously healthy woman, born in 19
A case report concerning the importance of the patients’ symptoms and clinical findings in the management of patients
Bodil Ohlsson
Gastroenterology Insights , 2010, DOI: 10.4081/gi.2010.e1
Abstract: The gastrointestinal tract has always been difficult to visualize in its entire length. New technology such as magnetic resonance imaging enterography, computed tomography ente-ro--graphy, single- and double-balloon entero-scopy, and video capsule enteroscopy have improved the possibilities for visualizing mucosal changes and pathology in the small intestine. We describe here a case of a patient with gastrointestinal signs and symptoms suggesting recurrent intestinal obstruction over a period of several years, who had mostly normal morphology on endoscopic examination. Nonetheless, after some delay, the patient underwent explorative surgery because of his accelerating symptoms. Abdominal exploration revealed a Meckel’s diverticulum, which had led to inflammatory adhesions of the small intestine with a sharp bend and proximal intestinal dilation. After the operation the patient’s health was restored. We concluded that in certain situations the clinician should rely on the patients’ symptoms and clinical findings more than on technological examinations and the clinical picture should guide clin-icians’ interventions, even though we live in a high-technique era.
Marginalization and Power in Living with and Researching Living with HIV
Bodil Pedersen
Outlines : Critical Practice Studies , 2005,
Abstract: This article takes its point of departure in a research project studying the psychosocial problems of living with HIV. The project was intended to participate in changing practices dealing with these problems. It became a project including many differently situated and intersecting personal and generalized perspectives. The article researches the development of the HIV project as a contribution to discussions related to Participatory Action Research and Practice Research. In mainstream approaches methodological indications are often presented as rules to follow in order to ensure the quality of the obtained knowledge. But situated historical and societal processes are involved in the effectuation of the HIV project, like they are in any other project. Researching the project heightens the awareness of the necessity of reflecting on situated and historical issues of power and marginalization and on the positions of the researcher in a given field of research. Methodological flexibility may also be necessary in order to encompass different perspectives. Such reflections and strategies are necessary precisely to ensure the development of knowledge and practice alike.
History in Popular Magazines: Negotiating Masculinities, the Low of the Popular and the High of History
Bodil Axelsson
Culture Unbound : Journal of Current Cultural Research , 2012,
Abstract: This article explores how the low of the popular and the high of history intersect to negotiate masculinities in the nexus of politics and war in a Swedish history magazine. It investigates the content of the magazine’s form and argues that it produces a kaleidoscopic take on the past which begs the reader to go along with the ads to buy another book, travel to one more historical site, buy a DVD or go to the movies, to turn the page, or to buy another issue of the magazine. Two articles, biographical in their outset, provide the basis for an analysis on how masculinities are negotiated by displaying political and military leaders in contradictory ways and enabling multiple entrance points for the contemporary reader and spectator. Articles on great men produce cultural imaginaries of warlords and political leaders by drawing on layers of historically contingent ways for men to act in public and private spheres and connecting late modern visual celebrity culture to the cults of fame in earlier centuries.
Auto-Antibodies and Their Association with Clinical Findings in Women Diagnosed with Microscopic Colitis
Bodil Roth, Rita J. Gustafsson, Bodil Ohlsson
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0066088
Abstract: Background Microscopic colitis (MC) is a disease manifested by diarrhoea and is divided into collagenous and lymphocytic colitis. The aetiology is unknown, but auto-immunity is suggested. Auto-antibodies have been only rarely examined in this entity. The aim of the study was to examine the prevalence of auto-antibodies, and to examine associations between the presence of antibodies and clinical findings. Methods and Findings Women with MC verified by biopsy and younger than 73 years, at any Department of Gastroenterology, in the district of Sk?ne, between 2002 and 2010 were invited to participate in this study. The patients were asked to complete both a questionnaire describing their medical history and the Gastrointestinal Symptom Rating Scale (GSRS). Blood samples were collected. Anti-nuclear antibodies (ANA), anti-neutrophil cytoplasmic antibodies (ANCA), anti-Saccharomyces cerevisiae antibodies (ASCA), and antibodies against glutamic acid decarboxylase (anti-GAD), islet antigens-like insulin 2 (anti-IA2), thyroid peroxidase (anti-TPO), and thyrotropin receptor (TRAK) were analysed. Of 240 women identified, 133 were finally included in the study, median age 63 (59–67) years. Apart from the MC diagnosis, 52% also suffered from irritable bowel syndrome, 31% from hypertension and 31% from allergy. The prevalence of ANA (14%), ASCA IgG (13%), and anti-TPO antibodies (14%) for these patients was slightly higher than for the general population, and were found together with other concomitant diseases. Patients had more of all gastrointestinal symptoms compared with norm values, irrespective of antibody expression. Conclusions Women with MC have a slightly increased prevalence of some auto-antibodies. These antibodies are not associated with symptoms, but are expressed in patients with concomitant diseases, obscuring the pathophysiology and clinical picture of MC.
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