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Search Results: 1 - 10 of 205224 matches for " Blanca Lilia Barrón Romero "
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Pathology Isolation and Identification of Canine Herpesvirus (CHV-1) in Mexico  [PDF]
E. Guillermo Valdivia Lara, Blanca Lilia Barrón Romero, Laura Cobos Marín, Jessica Ileana ángeles Solis, Sofía González Gallardo, Cesar Cuenca Verde, Juan Antonio Montaraz Crespo, Guillermo Valdivia Anda
Open Journal of Pathology (OJPathology) , 2016, DOI: 10.4236/ojpathology.2016.63014
Abstract: This work presents the pathology description, isolation and identification of canine herpesvirus (CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae family. Methods: Isolates were obtained from puppies that died within the first four weeks of life and had lesions consistent with canine herpesvirus. Results: The main gross lesions were petechial and ecchymotic hemorrhages in kidneys, liver and lungs; proliferative interstitial nephritis; multifocal necrosis in liver and kidneys; and encephalitis with intranuclear inclusion bodies. Herpesvirus was confirmed through direct immunofluorescence, electron microscopy and polymerase chain reaction for DNA polymerase and glycoprotein B genes. Discussion: Eight strains were isolated and identified as canine herpesvirus corresponding to three of the working cases with gross and microscopic lesions very similar to those described in the literature; then, isolates were confirmed by PCR gene amplification, positive reactions on immunofluorescence and observations from electron microscopy. This work represents the first report of this disease, including gross and histological lesions, and confirmation by isolation and identification of the canine herpesvirus in Mexico.
Interferencia de una fracción hemoaglutinante de veneno crotálico en la adsorción del virus de parainfl uenza-3 (VPIB-3)
Arcelia Alvarado Islas,ángel Horacio Sandoval Trujillo,Octavio de Paz Villafán,Blanca Lilia Barrón Romero
Veterinaria México , 2007,
Abstract: Se evaluó una fracción proteínica con actividad hemoaglutinante (fracción AL27), aislada del veneno de la serpiente Agkistrodon piscivorus, en su aptitud para inhibir in vitro al virus de parainfl uenza-3 bovino (VPIB-3), ya sea por su capacidad de bloquear receptores celulares o por su adherencia a las espículas de la hemoaglutinina viral. La adición de ≤ 1.062 μg/mL de AL27 en cultivos MDBK, previa a la infección con el VPIB-3 (título 105.6 DICC50%), impidió el da o o destrucción de los tejidos, manteniendo la viabilidad celular entre 69.43% y 84.86%. Asimismo, tras la mezcla de ambos reactantes en proporción 1:1 (1 mL AL27 en concentración ≤ 1.062 μg/mL + 1 mL VPIB-3 con título 105.6 DICC50%) e incubación a 37°C durante 1 h, se detectó al microscopio electrónico una aparente unión de AL27 con las espículas de hemoaglutinina virales. El bloqueo del VPIB-3 por AL27 se dedujo mediante la reducción del título viral de 105.6 a 102.0 DICC50%. Además, se detectó probable identidad entre AL27 y VPIB-3, por el reconocimiento de una proteína común de 21 kDa al realizar un Western blot con suero hiperinmune anti-Agkistrodon piscivorus. Los resultados muestran la factibilidad de que la fracción AL27 pueda ser empleada como agente antiviral.
Conjugate Gradient Algorithm for Solving a Optimal Multiply Control Problem on a System of Partial Differential Equations
Carlos Barrón-Romero
Mathematics , 2014,
Abstract: I development a Conjugate Gradient Method for solving a partial differential system with multiply controls. Some numerical results are depicted. Also, I present an explication of why the control over a partial differential equations system is necessary.
Classical and Quantum Algorithms for the Boolean Satisfiability Problem
Carlos Barrón-Romero
Computer Science , 2015,
Abstract: This paper presents a complete algorithmic study of the decision Boolean Satisfiability Problem under the classical computation and quantum computation theories. The paper depicts deterministic and probabilistic algorithms, propositions of their properties and the main result is that the problem has not an efficient algorithm (NP is not P). Novel quantum algorithms and propositions depict that the complexity by quantum computation approach for solving the Boolean Satisfiability Problem or any NP problem is lineal time.
El paciente diabético, aspectos psicológicos de su manejo
Lilia Portilla,Maria Isabel Romero,Jorge Román
Revista Latinoamericana de Psicología , 1991,
Abstract: El presente trabajo describe las reacciones psicológicas y sociales más comunes en los pacientes diabéticos y su posible influencia en el control metabólico. Se analizan las reacciones de inadaptación tributarias de atención especializada tales como el debut, el rechazo a la enfermedad, situaciones especiales en el curso de la misma, y otras. Se valoran algunas estrategias para su tratamiento
Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection
Rosete, Dora P;Manjarrez, María Eugenia;Barrón, Blanca L;
Memórias do Instituto Oswaldo Cruz , 2008, DOI: 10.1590/S0074-02762008000200012
Abstract: adenoviruses (adv) are commonly involved in acute respiratory infections (ari), which cause high morbidity and mortality in children. adv are grouped in six species (a-f), which are associated with a wide range of diseases. the aim of this study was to identify the adv species infecting non-hospitalized mexican children with ari symptoms, attending to the same school. for that, a pcr/rflp assay was designed for a region of the hexon gene, which was chosen, based on the bioinformatical analysis of adv genomes obtained from genbank. a total of 100 children's nasopharyngeal samples were collected from january to june, 2005, and used for viral isolation in a549 cells and pcr/rflp analysis. only 15 samples produced cytopathic effect, and in all of them adv c was identified. adv c was also identified in eight additional nasopharyngeal samples which were negative for viral isolation. in summary, this outpatient population showed a rate of adv infection of 23%, and only adv c was detected.
Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode
Borja Gheorghe M,Meza Mora Eugenio,Barrón Blanca,Gosset Guillermo
Microbial Cell Factories , 2012, DOI: 10.1186/1475-2859-11-132
Abstract: Background Plasmid DNA (pDNA) is a promising molecule for therapeutic applications. pDNA is produced by Escherichia coli in high cell-density cultivations (HCDC) using fed-batch mode. The typical limitations of such cultivations, including metabolic deviations like aerobic acetate production due to the existence of substrate gradients in large-scale bioreactors, remain as serious challenges for fast and effective pDNA production. We have previously demonstrated that the substitution of the phosphotransferase system by the over-expressed galactose permease for glucose uptake in E. coli (strain VH33) allows efficient growth, while strongly decreases acetate production. In the present work, additional genetic modifications were made to VH33 to further improve pDNA production. Several genes were deleted from strain VH33: the recA, deoR, nupG and endA genes were inactivated independently and in combination. The performance of the mutant strains was evaluated in shake flasks for the production of a 6.1 kb plasmid bearing an antigen gene against mumps. The best producer strain was cultivated in lab-scale bioreactors using 100 g/L of glucose to achieve HCDC in batch mode. For comparison, the widely used commercial strain DH5α, carrying the same plasmid, was also cultivated under the same conditions. Results The various mutations tested had different effects on the specific growth rate, glucose uptake rate, and pDNA yields (YP/X). The triple mutant VH33 Δ (recA deoR nupG) accumulated low amounts of acetate and resulted in the best YP/X (4.22 mg/g), whereas YP/X of strain VH33 only reached 1.16 mg/g. When cultivated at high glucose concentrations, the triple mutant strain produced 186 mg/L of pDNA, 40 g/L of biomass and only 2.2 g/L of acetate. In contrast, DH5α produced only 70 mg/L of pDNA and accumulated 9.5 g/L of acetate. Furthermore, the supercoiled fraction of the pDNA produced by the triple mutant was nearly constant throughout the cultivation. Conclusion The pDNA concentration obtained with the engineered strain VH33 Δ (recA deoR nupG) is, to the best of our knowledge, the highest reported for a batch cultivation, and its supercoiled fraction remained close to 80%. Strain VH33 Δ (recA deoR nupG) and its cultivation using elevated glucose concentrations represent an attractive technology for fast and efficient pDNA production and a valuable alternative to fed-batch cultivations of commercial strains.
Protein Quality Evaluation of Animal Food Proteins by In-Vitro Methodologies  [PDF]
Jesús Manuel Barrón-Hoyos, Adrian Rafael Archuleta, María del Refugio Falcón-Villa, Rafael Canett-Romero, Francisco Javier Cinco-Moroyoqui, Ana Lourdes Romero-Barancini, Edgar Omar Rueda-Puente
Food and Nutrition Sciences (FNS) , 2013, DOI: 10.4236/fns.2013.44048
Abstract:

Animal protein foods are undoubtedly among the most concentrated source of essential amino acids (AA) for the human diet. However, their high prices and diseases associated to their excessive consumption have fomented the consumption of other alternative sources of animal proteins such as those from marine or aquatic species. Sonora is a well recognized producer of animal foods in Mexico, both terrestrial and aquatic. In this study, the protein quality evaluation of these animal food sources, highly produced and consumed in Sonora, is proposed, using in-vitro methodologies. Four different species, from each aquatic and terrestrial origin, were selected. Samples of lean muscle were used in all cases. Various in-vitro methodologies for protein quality evaluation were selected, alternatives to the animal bioassays: % digestibility, Total amino acid analyses (HPLC), PDCAAS, computerized PER calculations (C-PER and DC-PER) and total collagen contents. % in-vitro digestibility presented significant differences among samples from terrestrial species, but muscle from aquatic species did not showed significant differences. All sources of proteins, both aquatic and terrestrial proved to be rich sources of essential amino acids. PDCAAS was unable to establish significant differences in protein quality among sources of protein from different origin. Both methods C-PER and DC-PER were more exact in their results and were able to detect significant differences among samples of different origin. An important finding was the great difference in the total collagen content between aquatic and terrestrial sources of proteins, where terrestrial muscle proteins had almost 10-time more collagen than aquatic protein sources. However, these collagen contents did not seem to have a significant influence in the protein quality of these animal proteins. These muscle proteins, from both aquatic and terrestrial species, confirmed to have a high protein quality and some of the in-vitro methodologies used in this study represent a valuable alternative to the animal bioassays.

POLYCYCLIC AROMATIC HYDROCARBONS IN SOILS FROM A BRICK MANUFACTURING LOCATION IN CENTRAL MEXICO
Ana Lilia BARRáN-BERDóN,Virgilio GARCíA GONZáLEZ,Gustavo PEDRAZA ABOYTES,Ismael RODEA-PALOMARES
Revista internacional de contaminación ambiental , 2012,
Abstract: Los hidrocarburos aromáticos policíclicos (HAP) están ampliamente distribuidos en el ambiente y su origen puede ser natural o antrópico. La mayoría de los HAP ambientales son productos de la combustión incompleta o pirólisis de combustibles fósiles y pueden ser introducidos al suelo vía depositación atmosférica. Los HAP son importantes para la salud pública y el ambiente debido a sus posibles efectos carcinogénicos y mutagénicos y a su influencia da ina en los organismos del suelo y las plantas. En este estudio se analizaron los niveles y composición de 13 HAP de 46 muestras de suelo de San Nicolás, una comunidad manufacturera de ladrillos en el centro de México. Las concentraciones totales de HAP estuvieron entre 7 y 1384 ng/g con un promedio de 220 ng/g. El HAP más abundante fue el naftaleno (Nap), seguido por el fluoreno (Fl), el criseno (Chr), el benzo[a]antraceno (BaA) y el dibenzo[a,h]antraceno (DahA). Al comparar los niveles de HAP en el suelo con las normas mexicanas y estadounidenses, se encontró que alrededor de 52 y 76 % de los suelos de San Nicolás tiene niveles más altos que los considerados normales o no peligrosos para uso residencial con base en cualquiera de las dos normas mencionadas. Más aún, las concentraciones equivalentes de benzo[a]pireno (BaPeq) en los suelos de San Nicolás fueron más altas que las máximas aceptables establecidas por Canadá en 40 a 60 % de los suelos. De especial preocupación son las concentraciones de DaH y BaP, dos HAP con un elevado potencial carcinogénico. Todos estos factores implican un riesgo potencial de cáncer para la población expuesta de San Nicolás.
Patrón de transporte de sedimento en Bahía Magdalena, Baja California Sur, México, inferido del análisis de tendencias granulométricas
Sánchez,Alberto; Choumiline,Evgueni; Estela López-Ortiz,Blanca; Aguí?iga,Sergio; Sánchez-Vargas,Lilia; Romero-Guadarrama,Armando; Rodríguez-Meza,Durga;
Latin american journal of aquatic research , 2010,
Abstract: the grain-size trend analysis (grain size, sorting asymmetry) was determined at 58 stations in order to infer the residual vectors of sediment transport in magdalena bay, baja california sur, mexico. grain-size trend analysis and principal components analysis were applied to textural sediment parameters. the results indicated that well-sorted sediment is associated with fine to very fine sand. the residual vector transport suggests the presence of two gyres in the dispersion of clastic material: (1) acycloneic gyre in the deeper, central part and (2) an acycloneic gyre in the southeast part of the bay. the sediment transport pattern is highly correlated with the residual tidal currents in magdalena bay.
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