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Search Results: 1 - 10 of 11045 matches for " Bing Siang Gan "
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Pain and Efficacy Rating of a Microprocessor-Controlled Metered Injection System for Local Anaesthesia in Minor Hand Surgery
André S. Nimigan,Bing Siang Gan
Pain Research and Treatment , 2011, DOI: 10.1155/2011/362396
Abstract: Purpose. Little attention has been given to syringe design and local anaesthetic administration methods. A microprocessor-controlled anaesthetic delivery device has become available that may minimize discomfort during injection. The purpose of this study was to document the pain experience associated with the use of this system and to compare it with use of a conventional syringe. Methods. A prospective, randomized clinical trial was designed. 40 patients undergoing carpal tunnel release were block randomized according to sex into a two groups: a traditional syringe group and a microprocessor-controlled device group. The primary outcome measure was surgical pain and local anaesthetic administration pain. Secondary outcomes included volume of anaesthetic used and injection time. Results. Analysis showed that equivalent anaesthesia was achieved in the microprocessor-controlled group despite using a significantly lower volume of local anaesthetic ( ). This same group, however, has significantly longer injection times ( ). Pain during the injection process or during surgery was not different between the two groups. Conclusions. This RCT comparing traditional and microprocessor controlled methods of administering local anaesthetic showed similar levels of discomfort in both groups. While the microprocessor-controlled group used less volume, the total time for the administration was significantly greater. 1. Introduction The use of local anaesthesia allows surgeons to perform minor surgery procedures in a variety of settings, including the emergency rooms and clinics. The first recorded nerve block was achieved by Halstead, who used cocaine to accomplish an inferior alveolar block on himself in 1884. Hollow tip hypodermic syringes were introduced not long after by Pravaz and Wood. Unfortunately, the administration of local anaesthesia in itself causes pain, despite attempts to diminish this anaesthesia-associated pain, such as by chemically modifying anaesthetic agents, adding buffering agents, or changing the anaesthetic temperature during administration. Very little attention has been given to the current syringe design and the administration methods, and effectively, syringe systems have changed a little since their introduction over a century ago [1]. A new development in the attempt to give greater operator control and minimize patient discomfort and distress is a product known as the Midwest Comfort Control System. This anaesthetic delivery device eliminates the variability of a thumb-operated plunger, allowing for maintenance of an ideal flow rate of
Molecular mechanisms and treatment strategies for Dupuytren’s disease
David B O’Gorman, Linda Vi, Bing Siang Gan
Therapeutics and Clinical Risk Management , 2010, DOI: http://dx.doi.org/10.2147/TCRM.S9165
Abstract: lecular mechanisms and treatment strategies for Dupuytren’s disease Review (3400) Total Article Views Authors: David B O’Gorman, Linda Vi, Bing Siang Gan Published Date August 2010 Volume 2010:6 Pages 383 - 390 DOI: http://dx.doi.org/10.2147/TCRM.S9165 David B O’Gorman1,2,3,4, Linda Vi1,2,5, Bing Siang Gan1,2,3,5,6 1Cell and Molecular Biology Laboratory, 2The Hand and Upper Limb Centre, St. Joseph’s Health Care London, Schulich School of Medicine and Dentistry, 3Departments of Surgery, 4Biochemistry, 5Physiology and Pharmacology, 6Medical Biophysics, The University of Western Ontario, London, OT, Canada Abstract: Dupuytren’s disease (DD) is a common disease of the hand and is characterized by thickening of the palmar fascia and formation of tight collagenous disease cords. At present, the disease is incurable and the molecular pathophysiology of DD is unknown. Surgery remains the most commonly used treatment for DD, but this requires extensive postoperative therapy and is associated with high rates of recurrence. Over the past decades, more indepth exploration of the molecular basis of DD has raised the hopes of developing new treatment modalities. This paper reviews the clinical presentation and molecular pathophysiology of this disease, as well as current and emerging treatment. It also explores the implications of new findings in the laboratory for future treatment.
Update on the management of Dupuytren’s contracture
Linda Vi, David B O’Gorman, Bing Siang Gan
Orthopedic Research and Reviews , 2010, DOI: http://dx.doi.org/10.2147/ORR.S8592
Abstract: ate on the management of Dupuytren’s contracture Review (4979) Total Article Views Authors: Linda Vi, David B O’Gorman, Bing Siang Gan Published Date August 2010 Volume 2010:2 Pages 35 - 43 DOI: http://dx.doi.org/10.2147/ORR.S8592 Linda Vi1, David B O’Gorman2, Bing Siang Gan3 1Department of Physiology and Pharmacology, 2Hand and Upper Limb Centre, Lawson Health Research Institute, Departments of Surgery and Biochemistry, 3Hand and Upper Limb Centre, Lawson Health Research Institute, Departments of Surgery and Medical Biophysics, University of Western Ontario, London, Ontario, Canada Abstract: Dupuytren’s disease (DD) is a pathological condition of the palmar fascia that is characterized by the formation of tight collagenous disease cords leading to permanent finger contractures. The disease is most prevalent in Caucasian men, and its incidence increases with age advancement. The most common complaint from patients having DD is the impairment of normal hand function. At present, the disease is incurable and the pathophysiology of DD is unknown. The most common treatment for DD is surgery; however, this treatment is associated with a high rate of recurrence. More recently, researchers have begun to explore the molecular basis of DD in the hopes of developing new, more effective treatment for DD. This review will summarize the history and clinical presentation of the disease, highlight current and emerging molecular treatments, and explore the implications of these advancements for future work.
Update on the management of Dupuytren’s contracture
Linda Vi,David B O’Gorman,Bing Siang Gan
Orthopedic Research and Reviews , 2010,
Abstract: Linda Vi1, David B O’Gorman2, Bing Siang Gan31Department of Physiology and Pharmacology, 2Hand and Upper Limb Centre, Lawson Health Research Institute, Departments of Surgery and Biochemistry, 3Hand and Upper Limb Centre, Lawson Health Research Institute, Departments of Surgery and Medical Biophysics, University of Western Ontario, London, Ontario, CanadaAbstract: Dupuytren’s disease (DD) is a pathological condition of the palmar fascia that is characterized by the formation of tight collagenous disease cords leading to permanent finger contractures. The disease is most prevalent in Caucasian men, and its incidence increases with age advancement. The most common complaint from patients having DD is the impairment of normal hand function. At present, the disease is incurable and the pathophysiology of DD is unknown. The most common treatment for DD is surgery; however, this treatment is associated with a high rate of recurrence. More recently, researchers have begun to explore the molecular basis of DD in the hopes of developing new, more effective treatment for DD. This review will summarize the history and clinical presentation of the disease, highlight current and emerging molecular treatments, and explore the implications of these advancements for future work.Keywords: Dupuytren’s disease, etiology, clinical presentation, treatment
Molecular mechanisms and treatment strategies for Dupuytren’s disease
David B O’Gorman,Linda Vi,Bing Siang Gan
Therapeutics and Clinical Risk Management , 2010,
Abstract: David B O’Gorman1,2,3,4, Linda Vi1,2,5, Bing Siang Gan1,2,3,5,61Cell and Molecular Biology Laboratory, 2The Hand and Upper Limb Centre, St. Joseph’s Health Care London, Schulich School of Medicine and Dentistry, 3Departments of Surgery, 4Biochemistry, 5Physiology and Pharmacology, 6Medical Biophysics, The University of Western Ontario, London, OT, CanadaAbstract: Dupuytren’s disease (DD) is a common disease of the hand and is characterized by thickening of the palmar fascia and formation of tight collagenous disease cords. At present, the disease is incurable and the molecular pathophysiology of DD is unknown. Surgery remains the most commonly used treatment for DD, but this requires extensive postoperative therapy and is associated with high rates of recurrence. Over the past decades, more indepth exploration of the molecular basis of DD has raised the hopes of developing new treatment modalities. This paper reviews the clinical presentation and molecular pathophysiology of this disease, as well as current and emerging treatment. It also explores the implications of new findings in the laboratory for future treatment.Keywords: Dupuytren’s contracture, Dupuytren’s disease, fibrosis
A novel mass spectrometry-based assay for GSK-3β activity
Erin Bowley, Erin Mulvihill, Jeffrey C Howard, Brian J Pak, Bing Siang Gan, David B O'Gorman
BMC Biochemistry , 2005, DOI: 10.1186/1471-2091-6-29
Abstract: Synthetic peptide substrates designed with a GSK-3β phosphorylation site were assayed with both recombinant enzyme and GSK-3β immunoprecipitated from NIH 3T3 fibroblasts. A molecular weight shift equal to that of a single phosphate group (80 Da.) was detected by surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) in a GSK-3β target peptide (2B-Sp). Not only was there a dose-dependent response in molecular weight shift to the amount of recombinant GSK-3β used in this assay, this shift was also inhibited by lithium chloride (LiCl), in a dose-dependent manner.We present here a novel method to sensitively measure peptide phosphorylation by GSK-3β that, due to the incorporation of substrate controls, is applicable to either purified enzyme or cell extracts. Future studies using this method have the potential to elucidate the activity of GSK-3β in vivo, and to screen enzyme activity in relation to a variety of GSK-3β related disorders.Phosphorylation is believed to be the most common protein post-translational covalent modification and is known to occur in the processing of as many as 1/3 of eukaryotic gene products [1]. That the mammalian genome is predicted to encode as many as 1000 different protein phosphatases and twice as many kinases underlines the importance of protein phosphorylation in cellular function [2,3]. One of the most diverse protein kinases studied to-date is the constitutively active serine/threonine kinase, Glycogen Synthase Kinase-3beta (GSK-3β). Originally identified for its role in the regulation of glycogen metabolism [4], it is now known that GSK-3β plays a key role in cellular processes as diverse as cytoskeletal regulation [5], cell cycle progression [6,7], apoptosis [8], cell fate and specification [9], and transcriptional/translational initiation [10,11]. Therefore, functional kinase activity of GSK-3β is important in a variety of biological and biochemical processes and altered GSK-3β activity can con
Enhanced Dupuytren's disease fibroblast populated collagen lattice contraction is independent of endogenous active TGF-β2
Raymond Tse, Jeffrey Howard, Yan Wu, Bing Gan
BMC Musculoskeletal Disorders , 2004, DOI: 10.1186/1471-2474-5-41
Abstract: Fibroblast-populated collagen lattice (FPCL) contraction assays using primary cell cultures derived from diseased and control fascia of the same DD patients were studied in response to exogenous TGF-β2 and neutralizing anti-TGF-β2 antibodies.Contraction of the FPCLs occurred significantly faster and to a greater extent in disease cells compared to control cells. The addition of TGF-β2 enhanced the rate and degree of collagen contraction in a dose-dependent fashion for both control and diseased cells. Neutralizing anti-TGF-β2 antibodies abolished exogenous TGF-β2 stimulated collagen contraction, but did not inhibit the enhanced basal collagen contraction activity of disease FPCL cultures.Although exogenous TGF-β2 stimulated both disease and control FPCL contraction, neutralizing anti-TGF-β2 antibodies did not affect the elevated basal collagen contraction activity of disease FPCLs, suggesting that the differences in the collagen contraction activity of control and disease FPCL cultures are not due to differences in the levels of endogenous TGF-β2 activity.Dupuytren's disease (DD) is a fibro-proliferative disorder of the palmar fascia (PF) characterized by the formation of fibrous nodules and cords [1]. The disease results in digital contractures, leading to loss of hand function. Surgical excision of the diseased PF is currently the principal form of management since the lack of a clear etiology has precluded the development of other effective and rational forms of treatment.Since Baron Guillaume Dupuytren's classical description of the disease in 1831, multiple clinical associations have been described, however, no clear molecular mechanism for the disease has been established [2]. Histochemical studies of DD have demonstrated the presence of myofibroblasts [3], increased production of type III collagen [4-7], and alterations in other extra-cellular matrix proteins including various fibronectin isoforms [8-14]. These biological features are characteristic of abnorma
Dimerization of two novel apoptosisinducing proteins and its function in regulating cell apoptosis
Qingzhen Liu,Miao Gan,Yipeng Qi,Lingyun Li,Bing Qi
Science China Life Sciences , 2003, DOI: 10.1360/03yc9024
Abstract: Asy (apoptosis/saibousi Yutsudo) is a novel apoptosis-inducing gene found in 1999 by Yutsudo group in Japan. In 2000, Qi Bing et al. cloned another novel gene, named hap (homologue of ASY protein), which encoded the ASY interacting protein, from human lung cell line (WI-38) cDNA library by using yeast two-hybrid system. It has been proved that ASY formed homodimer in yeast and human cell line, ASY and HAP formed heterodimer in yeast cells, and both induced cell apoptosis in human tumor cell lines Sao2 and CGL4. This paper showed that HAP could form homodimer in yeast cells by yeast two-hybrid system; HAP and ASY could produce heterodimer in human cell line by cross-immunoprecipitation test; by using apoptosis-testing technologies such as AnnexinV, TUNEL, DNA ladder and Flow Cytometry, the cell apoptosis in human normal or tumor cell lines transfected with hap or asy individually or cotransfected by the both was qualified or quantified. It was firstly demonstrated that ASY or HAP induced cell apoptosis not only in human tumor cell lines, but also in human normal cell lines. Moreover, we proved that the heterodimer between ASY and HAP decreased apoptosis-inducing activity from the homodimer of ASY or HAP. It revealed that by choosing to form heterodimer or homodimer between ASY and / or HAP is an important mechanism of regulating apoptosis in human cell lines.
Identification of hot spot residues at protein-protein interface
Lei Li,Bing Zhao,Zhanhua Cui,Jacob Gan
Bioinformation , 2006,
Abstract: It is known that binding free energy of protein-protein interaction is mainly contributed by hot spot (high energy) interface residues. Here, we investigate the characteristics of hot spots by examining inter-atomic sidechain-sidechain interactions using a dataset of 296 alanine-mutated interface residues. Results show that hot spots participate in strong and energetically favorable sidechain-sidechain interactions. Subsequently, we describe a novel, yet simple ‘hot spot’ prediction model with an accuracy that is similar to many available approaches. The model is also shown to efficiently distinguish specific protein-protein interactions from non-specific interactions.
PHYLOGENETIC RELATIONSHIPS AND ITS MORPHOLOGICAL CHARACTERISTICS AMONG SOME PYTHIUM SPECIES INFERRED FROM SEQUENCE ANALYSIS OF rDNA ITS
基于rDNA ITS序列探讨部分腐霉种的系统发育与其形态特征

LOU Bing-Gan ZHANG Bing-Xin,
楼兵干
,张炳欣

菌物学报 , 2005,
Abstract: 基于对73株计58种腐霉和6种疫霉的核糖体DNA的ITS序列分析,以海生疫霉为外围群,按邻接法构建系统发育树,对腐霉的系统发育关系进行了研究。结果表明:在58种腐霉中,Pythium ostracodes,P.chamaehyphon,Pcarbonicum,P.montanum和P.vexans归为同一组,介于其它腐霉和疫霉之间,这5种腐霉的孢子囊均为球形;现已归为疫霉属的P.undulatum单独为一组,它与腐霉的亲缘关系比疫霉更近;其余52种腐霉聚成一大组,这52种腐霉基本上按孢子囊或菌丝膨大体形态分成Ⅰ、Ⅱ两组:第Ⅰ组31种腐霉,其中30种腐霉的孢子囊或菌丝膨大体为球形;第Ⅱ组21种腐霉,其中19种腐霉的孢子囊为丝状、瓣状或裂片状。基于ITS序列分析,腐霉属的其它性状如藏卵器壁是否光滑、卵孢子是否满器、雄器的着生方式和数量、异宗配合等呈多元演化。
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