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Search Results: 1 - 10 of 303850 matches for " Beatriz Madalena J; "
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In vitro organogenesis of zucchini squash cv. Caserta
Stipp, Liliane Cristina L;Monteiro-Hara, Alessandra Cristina BA;Mendes, Beatriz Madalena J;
Horticultura Brasileira , 2012, DOI: 10.1590/S0102-05362012000200015
Abstract: a protocol for the in vitro culture of cucurbita pepo cv. caserta was studied, using a cotyledon segment with an attached hypocotyl fragment as an explant. first, to determine the optimal seedling age, explants were collected from 4 to 6-day-old in vitro germinated seedlings and cultured in ms basal medium supplemented with benzylaminopurine (bap, 4.5 μm), under a 16-h photoperiod at 27oc. based on the results obtained, the explants collected from the 4-day-old seedlings were then cultured in ms basal medium supplemented with different concentrations of bap (0, 1.1, 2.2, 3.3, 4.5, or 5.5 μm) and incubated under a 16-h photoperiod at 27oc. in vitro organogenesis was most efficient with explants collected from 4-day-old seedlings cultured in medium supplemented with 4.5 μm of bap. after 4 weeks of incubation the development of adventitious buds at the cotyledon/hypocotyl junction could be observed. these buds were transferred to elongation and rooting medium and the developed plants were acclimatized to greenhouse conditions. the morphogenic process was characterized using light and scanning electron microscopy analyses to confirm the organogenesis. the results showed that this alternate explant is efficient for in vitro culture of zucchini squash cv. caserta. the protocol will be further examined for future use in genetic transformation experiments in this species.
Optimization of in vitro organogenesis in passion fruit (Passiflora edulis f. flavicarpa)
Trevisan, Flavio;Mendes, Beatriz Madalena Januzzi;
Scientia Agricola , 2005, DOI: 10.1590/S0103-90162005000400007
Abstract: in vitro organogenesis of passion fruit was studied by the induction of adventitious buds from leaf discs in culture media supplemented with benzyladenine (bap) or thidiazuron (tdz). to minimize adverse effects of ethylene accumulation on shoot development, silver nitrate (agno3) was added to the induction media. both bap (0; 2.2; 4.4; 6.6 μmol l-1) and tdz (0; 1.1; 2.2; 3.4 μmol l-1) were effective in promoting shoot development. although no significant differences were detected using agno3 (23.5 μmol l-1), buds grown in agno3-supplemented media were more vigorous. the number of explants with buds obtained using tdz and agno3-supplemented media (5.6) were higher than those obtained using bap and agno3 (3.0). msm + giberrellic acid (ga3), msm + coconut water, and ? msm culture media were tested for shoot bud elongation, incubated in flasks covered with either non-vented or vented lids. best results were obtained by culturing buds in msm + coconut water media in flasks covered with vented lids. plantlets transferred to msm + indol butyric acid (iba) media rooted in a 30-day period. passion fruit organogenesis was enhanced by using tdz and agno3 for bud induction. transferring the buds to msm + coconut water media and incubating in flasks with vented lids favored shoot elongation and plantlet development.
Avalia o econ mica e ambiental da energia at mica no Brasil = Economical and environmental evaluation of the atomic energy in Brazil
Juliana Bento,Gelta Madalena J?nck Pedroso
Acta Scientiarum : Technology , 2009,
Abstract: O objetivo desta pesquisa é analisar se a energia nuclear pode contribuir para gera o de energia elétrica de maneira sustentável no território nacional. O estudo foi realizado a partir de pesquisa bibliográfica e documental do setor energético, com levantamento de custos e impactos ambientais no processo de gera o de energia elétrica, nas usinas termonucleares. A emiss o de gases na atmosfera é elevada, e uma das principais causas de mudan as climáticas s o as usinas de carv o. Já no caso das usinas termonucleares,a emiss o é controlada, n o afetando o meio ambiente e a saúde humana. Inúmeras melhorias ainda s o necessárias para que seu ingresso flua como uma alternativa viável, de modo que sane problemas de escassez de matéria-prima, torne-se potencial fonte geradorade energia elétrica, n o coloque em risco a sobrevivência da humanidade e garanta a preserva o dos recursos naturais. The objective of this research is to analyze whether nuclear energy can contribute to the generation of electric power in a sustainable way in Brazil. The study was accomplished starting from a bibliographical and documental research of the energy sector,with a study of costs and environmental impacts in the process of electric power generation, in nuclear power plants. The emission of gases in the atmosphere is elevated, and one of the main causes of climatic changes is coal plants. In the case of nuclear power plants the emission is already controlled, not affecting the environment and human health. Countless improvements are still necessary so that it can become a viable alternative, solving problems of shortage of raw materials, becoming a potential generating source of electric power, not putting at risk the survival of humanity and guaranteeing the preservation of natural resources.
Uso de mutagênese "in vitro" no melhoramento de citros: I. Sensibilidade a raios gama de explantes do cultivar pêra
Tulmann Neto, Augusto;Cristofani, Mariangela;Mendes, Beatriz Madalena Januzzi;Ando, Akihiko;
Bragantia , 1994, DOI: 10.1590/S0006-87051994000200007
Abstract: in order to obtain basic data for future research in mutation breeding through "in vitro" technology in sweet orange cv. pêra, gamma rays sensitivity of different types of explants was analyzed. after irradiation the following observations were carried out: response of explants, number of embryoids and adventitious buds, callus weight and rooting formation. different results were obtained according the explant used. for irradiated nucellus, the dose was 20-40 gy and for embryogenic callus 120 gy is indicated. for irradiation of cotyledons originated from nucellar embryoids the use of 40 gy of gamma rays was recommended.
Indu??o e cultivo in vitro de gemas adventícias em segmentos de epicótilo de laranja-azeda
Silva, Rosely Pereira da;Mendes, Beatriz Madalena Januzzi;Mour?o Filho, Francisco de Assis Alves;
Pesquisa Agropecuária Brasileira , 2008, DOI: 10.1590/S0100-204X2008001000011
Abstract: the objective of this work was to evaluate the induction and formation of adventitious buds in sour orange explants through the use of plant regulators. in vitro organogenesis experiments were conducted to evaluate the effect of bap, tdz, and kin in different concentrations and under two light conditions; bap and kin, combined or not with naa; bap and kin, separately or in combined concentrations. sour orange epicotyl segments (1 cm length), from in vitro germinated plants, were used as explants. in order to induce bud formation, the explants were cultured in mt medium with or without the addition of plant regulators. the material was cultivated at 27oc in the absence of light for 30 days, followed of culture under a 16-hour photoperiod. the experimental design was completely randomized, with four or five replicates, depending on the experiment; each replicate comprised one petri dish with 20 explants. after 60 or 70 days of cultivation, the percentage of responsive explants and the number of buds were evaluated. the addition of bap to the culture medium, combined or not with ana, and in combinations with cin, induces better organogenic response.
Plant regeneration from protoplast of Brazilian citrus cultivars
GLORIA, FERNANDA JANUZZI MENDES DA;MOUR?O FILHO, FRANCISCO DE ASSIS ALVES;MENDES, BEATRIZ MADALENA JANUZZI;
Pesquisa Agropecuária Brasileira , 2000, DOI: 10.1590/S0100-204X2000000400008
Abstract: a procedure is described to regenerate plants from protoplasts of brazilian citrus cultivars, after isolation, fusion and culture. protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in vitro. the enzyme solution for protoplast isolation was composed of mannitol (0.7 m), cacl2 (24.5 mm), nah2po4 (0.92 mm), mes (6.15 mm), cellulase (onozuka rs - yakult, 1%), macerase (onozuka r10 - yakult, 1%) and pectolyase y-23 (seishin, 0.2%). protoplast culture in liquid medium after chemical fusion lead to the formation of callus colonies further adapted to solid medium. somatic embryo formation occurred spontaneously after two subcultures, on modified mt medium supplemented with 500 mg/l of malt extract. well defined embryos were germinated in modified mt medium with addition of ga3 (2.0 mm) and malt extract (500 mg/l). plant regeneration was also achieved by adventitious shoots obtained through direct organogenesis of not well defined embryos in modified mt medium with addition of malt extract (500 mg/l), bap (1.32 mm), naa (1.07 mm) and coconut water (10 ml/l). plantlets were transferred to root medium. rooted plants were transferred to a greenhouse for further adaptation and development.
Eficiência de isolamento e de plaqueamento de protoplastos de laranja-doce
Castro, Lívia Mendes de;Mour?o Filho, Francisco De Assis Alves;Mendes, Beatriz Madalena Januzzi;
Revista Brasileira de Fruticultura , 2011, DOI: 10.1590/S0100-29452011005000074
Abstract: protoplast isolation and culture are important factors for adequate in vitro culture of this type of explant to further genetic manipulations. the composition of the enzymatic solution, protoplast platting density, and plant genotype are important variables in these steps. therefore, this work aimed to evaluate the isolation efficiency of protoplasts related to three enzymatic solutions and platting efficiency of protoplasts based on five cell densities in different culture media compositions of sweet oranges cultivars. the enzymatic solutions tested were: 1. celulase onozuka rs 1%, macerase r-10 1% and pectoliase 0,2%; 2. celulase onozuka rs 1%, macerase r-10 1% ; 3. celulase onozuka r-10 4% , macerase r-10 1%. protoplasts were cultured at densities of 2 x 104; 5 x 104; 105; 2x 105 e 3 x 105 protoplasts.ml-1 in eme 0,7m, bh3 0,7m e bh3 + eme 0,7m, in darkness, at 25 ± 1 oc. the enzymatic solution 2 provided higher yield for 'hamlin', 'natal' and 'pêra' sweet orange cultivars, and enzymatic solution 1 resulted in better protoplast isolation for 'westin' sweet orange. for 'lima verde' sweet orange, enzymatic solution 3 was the most efficient. final platting efficiency, evaluated 90 days after culture, was higher at the densities of 3 x 105 e 2 x 105 protoplasts.ml-1 for 'hamlin', 'natal', and 'lima verde' sweet orange cultivars, and at the density of 2 x 105 e 105 protoplasts. ml-1 for 'westin' sweet orange.
Enraizamento de estacas do híbrido somático laranja 'caipira' + lim?o 'Volkameriano' e de seus genitores
Bassan, Meire Menezes;Mour?o Filho, Francisco de Assis Alves;Mendes, Beatriz Madalena Januzzi;
Revista Brasileira de Fruticultura , 2009, DOI: 10.1590/S0100-29452009000200041
Abstract: this work aimed to compare the rooting capacity of cuttings of the somatic hybrid 'caipira' sweet orange (c. sinensis l. osbeck) + 'volkamer' lemon (c. volkameriana v. tennore et pasquale) with those of their progenitors treated with indol butyric acid (iba) (0, 500, 1000 and 2000 mg l-1). cuttings of 'caipira' sweet orange and 'volkamer' lemon were collected from seed derived plants, whereas those of the somatic hybrid were obtained from recently pruned grafted plants. the experimental design was a completely randomized block 3 x 4 factorial (genotypes x iba concentrations), with four replications (10 cuttings per replication) in a total of 480 cuttings. cuttings were cultivated in expanded polystyrene trays filled with commercial potting mix, inside an intermittent mist chamber, for 90 days. the production of the rooted plants was highly efficient for the three genotypes evaluated, considering most of the variables related with rooting and cutting vigor, regardless the use of iba. 'volkamer' lemon cuttings had higher root length and root dry mass than the other genotypes. however, percentage of live cuttings, rooted cuttings and sprouted cuttings were high (above 90%) for all evaluated genotypes, indicating the great potential of this material to be used in nursery tree multiplication through cuttings.
Gene silencing: concepts, applications, and perspectives in woody plants
Souza, Amancio José de;Mendes, Beatriz Madalena Januzzi;Mour?o Filho, Francisco de Assis Alves;
Scientia Agricola , 2007, DOI: 10.1590/S0103-90162007000600014
Abstract: rna interference, transcriptional gene silencing, virus induced gene silencing, and micro rnas comprise a series of mechanisms capable of suppressing gene expression in plants. these mechanisms reveal similar biochemical pathways and appear to be related in several levels. the ability to manipulate gene silencing has produced transgenic plants able to switch off endogenous genes and invading nucleic acids. this powerful biotechnological tool has provided plant breeders and researchers with great opportunity to accelerate breeding programs and developmental studies in woody plants. this research work reports on gene silencing in woody plants, and discuss applications and future perspectives.
Calogênese, embriogênese somática e isolamento de protoplastos em variedades de laranja doce
Benedito, Vagner Augusto;Mour?o Filho, Francisco de Assis Alves;Mendes, Beatriz Madalena Januzzi;
Scientia Agricola , 2000, DOI: 10.1590/S0103-90162000000100007
Abstract: intending to produce embryogenic citrus calli (citrus sinensis l. osbeck), aborted ovules of ripe fruits from six sweet orange varieties (?bahia cabula?, ?baianinha?, ?hamlin?, ?orvalho de mel?, ?rubi? and ?valencia?) were introduced onto modified mt medium with and without benzyladenine (ba). calli obtained from ?bahia cabula?, ?orvalho de mel?, ?rubi? and ?valencia? varieties were grown on mt basal medium modified with the carbohydrates maltose, galactose, lactose, glucose and sucrose at 18; 37; 75; 110 and 150 mm. the effect on somatic embryogenesis was studied. calli from the same genotypes were submitted to protoplast isolation, using three different enzymatic solutions. results revealed the genotype effect in the callus induction. somatic embryogenesis was stimulated in the presence of galactose, for most varieties. enzymatic solution composed of 1% cellulase, 1% macerase and 0,2% pectolyase was the best for protoplast isolation from calli of all genotypes tested.
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