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Search Results: 1 - 10 of 196453 matches for " Barbara;D?bereiner "
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Adherence and experimental infection of bacteria associated with periodontal infections of young cattle in Brazil ("Cara inchada")
Grassmann, Barbara;Dbereiner, Jürgen;Dutra, Iveraldo S.;Kopp, Peter A.;Blobel, Hans;
Pesquisa Veterinária Brasileira , 1997, DOI: 10.1590/S0100-736X1997000300006
Abstract: in vitro- and in vivo-assays were conducted, to study the possible role of streptomycin- and actinomycin-producing soil actinomycetes for the pathogenesis of "cara inchada" in cattle (ci). adherence of bacteroides spp. to epithelial cells of the bovine gingiva, known to be associated with the progressive lesions of ci, was significantly increased by the addition of streptomycin, actinomycin or antibiotic culture supernatants of the soil actinomycetes. applications of these mixtures together with actinomyces pyogenes to the marginal gingiva of the upper premolar teeth of about 1 month old holstein friesian calves did not lead to progressive lesions of ci. only one calf exhibited a slight diarrhea and a temporary retraction of the gingiva at the site of application.
Adherence and experimental infection of bacteria associated with periodontal infections of young cattle in Brazil ("Cara inchada")
Grassmann Barbara,Dbereiner Jürgen,Dutra Iveraldo S.,Kopp Peter A.
Pesquisa Veterinária Brasileira , 1997,
Abstract: In vitro- and in vivo-assays were conducted, to study the possible role of streptomycin- and actinomycin-producing soil actinomycetes for the pathogenesis of "Cara inchada" in cattle (CI). Adherence of Bacteroides spp. to epithelial cells of the bovine gingiva, known to be associated with the progressive lesions of CI, was significantly increased by the addition of streptomycin, actinomycin or antibiotic culture supernatants of the soil actinomycetes. Applications of these mixtures together with Actinomyces pyogenes to the marginal gingiva of the upper premolar teeth of about 1 month old Holstein Friesian calves did not lead to progressive lesions of CI. Only one calf exhibited a slight diarrhea and a temporary retraction of the gingiva at the site of application.
Are alveolar bone changes a determinant factor for "cara inchada" in cattle?
Dbereiner, Jürgen;D?mmrich, Klaus;
Pesquisa Veterinária Brasileira , 1997, DOI: 10.1590/S0100-736X1997000200001
Abstract: in order to study possible alterations of the skeleton which might play a role in the pathogenesis of the periodontitis of "cara inchada" in young cattle, ribs from 20 affected calves, 2 to 10 months old, were examined. electrolytically decalcified longitudinal sections of the costochondral junction and cross sections through the corpus costae, stained with haematoxylin-eosin, were studied. in five calves, longitudinal sections of the proximal humerus were examined as well. the status of mineralization was checked by microradiograms. systemic alteration of the skeleton due to disturbances of mineral metabolism could not be shown in any of the animals. in seven 2 to 4 months old calves, no bone changes were found. the reduced osteogenesis in six 3 to 5 months old calves and the reduced osteogenesis and diminished chondral growth in seven 5 to 10 months old calves are therefore a consequence of the disease. the results show that the development of the alveolar bone was not defective, so this cannot be a determinant factor for the development of the periodontitis of "cara inchada" in cattle.
Are alveolar bone changes a determinant factor for "cara inchada" in cattle?
Dbereiner Jürgen,D?mmrich Klaus
Pesquisa Veterinária Brasileira , 1997,
Abstract: In order to study possible alterations of the skeleton which might play a role in the pathogenesis of the periodontitis of "cara inchada" in young cattle, ribs from 20 affected calves, 2 to 10 months old, were examined. Electrolytically decalcified longitudinal sections of the costochondral junction and cross sections through the corpus costae, stained with Haematoxylin-Eosin, were studied. In five calves, longitudinal sections of the proximal humerus were examined as well. The status of mineralization was checked by microradiograms. Systemic alteration of the skeleton due to disturbances of mineral metabolism could not be shown in any of the animals. In seven 2 to 4 months old calves, no bone changes were found. The reduced osteogenesis in six 3 to 5 months old calves and the reduced osteogenesis and diminished chondral growth in seven 5 to 10 months old calves are therefore a consequence of the disease. The results show that the development of the alveolar bone was not defective, so this cannot be a determinant factor for the development of the periodontitis of "cara inchada" in cattle.
Sobre o "ronca", doen?a de etiologia obscura em bovinos, caracterizada por respira??o ruidosa
Tokarnia, Carlos Hubinger;Dbereiner, Jürgen;
Pesquisa Veterinária Brasileira , 1998, DOI: 10.1590/S0100-736X1998000300001
Abstract: "ronca", a disease of cattle occurring in the states of piauí, rio de janeiro and mato grosso do sul, is also known by the popular names "roncadeira" or "ronqueira", what means snoaring disease. data on the disease were obtained through informations, own observations, clinical and postmortem examinations of 12 affected bovines, completed by histopathological examinations and chemical analyses of liver samples for microelements. the main symptoms are the snoaring sound during inspiration, which arises or is intensified the more one leades with the animal, irritability, continuous worsening of the nutritional state and death after a few months up to 2 years. blood examinations, performed during the studies in piauí, showed a normocytic and hypocromic condition; blood smears did not reveal any abnormality. at postmortem examination sometimes an orange discolouration of the liver and an orange-brown colour of the lymphnodes of the liver hilus was observed. careful examinations of the nasal cavity, especially of the turbinates, and of the larynx did not disclose any lesions. histopathological examinations revealed, as the main lesion, severe hemosiderosis of the liver, always seen, as well as of the spleen and lymphnodes. chemical analyses of liver samples revealed in all by "ronca" affected animals of the three regions very low copper and very high iron values. two bovines affected by "ronca" and transferred to a region where the disease does not occur, continued with the snoaring respiration during more than a year. at postmortem examination no lesions besides a few not related to the disease were found. histopathological examinations revealed hemosiderosis in liver, spleen and lymphnodes, but less pronounced than in the animals affected by "ronca" which stayed at the original farm. chemical anlyses of the liver samples of the two transferred animals revealed normal values for copper and high levels of iron, but these were lower than those found in the animals affected
IMUNIDADE CRUZADA PELAS SEMENTES DE Abrus precatorius E Ricinus communis EM BOVINOS
Tokarnia, Carlos Hubinger;Dbereiner, Jürgen;
Pesquisa Veterinária Brasileira , 1997, DOI: 10.1590/S0100-736X1997000100005
Abstract: five bovines immunized against the toxicity of the seeds of abrus precatorius l. (jequiriti bean) did not get poisoned or only slightly so when the seeds of ricinus communis l. (castor bean) were given in amounts that in bovines, which never before ingested the seeds of a. precatorius or r. communis, caused moderate to severe poisoning or even death. a sixth bovine, which was not well immunized against the toxic action of the seeds of a. precatorius, was severely poisoned when given a high dose of the seeds of r. communis. on the other side, from five bovines immunized against the toxic action of the seeds of r. communis four were severely poisoned and the fifth one moderately when the seeds of a. precatorius were given in doses that in bovines, which never before ingested seeds of r. communis or a. precatorius, caused slight to severe poisoning. it is concluded that bovines immunized against the toxic action of the seeds of a. precatorius are resistant to the toxic action of the seeds of r. communis, but that the contrary is not the case; this is, bovines immunized against the toxic action of the seeds of r. communis were not protected against the poisoning by a. precatorius. earlier studies by other authors had shown that the toxalbumins of the seeds of a. precatorius and r. communis, respectively abrin and ricin, are different as to their antigenic properties. a possible explanation for the difference in results can be.that in the present study polygastric animals were used which received the seeds orally, but in the earlier studies monogastric animals received the seeds or toxins by parenteral route. the administration of the fresh leaves or the pericarp of the fruits r. communis to bovines, which had been immunized against the action of the seeds of the plant, had the same toxic effect as to not immunized animals, showing that the immunity due to ricin does not give protection against the action of ricinin, the toxic principle of the leaves and the pericarp.
IMUNIDADE CRUZADA PELAS SEMENTES DE Abrus precatorius E Ricinus communis EM BOVINOS
Tokarnia Carlos Hubinger,Dbereiner Jürgen
Pesquisa Veterinária Brasileira , 1997,
Abstract: Cinco bovinos imunizados contra a a o tóxica das sementes de Abrus precatorius L. ("tento", "jiquiriti") n o adoeceram ou somente levemente pela administra o das sementes de Ricinus communis L. ("mamona"), em doses que em bovinos que antes nunca ingeriram sementes de A. precatorius ou R. communis, causaram intoxica o de intensidade de grau moderado a acentuado ou até a morte. Um sexto bovino, que n o ficou bem imunizado contra a a o tóxica das sementes de A. precatorius, adoeceu em grau acentuado pela administra o de dose elevada das sementes de R. communis. Já dos cinco bovinos imunizados contra a a o tóxica das sementes de R. communis quatro adoeceram em grau acentuado, oquinto em grau moderado, pela administra o das sementes de A. precatorias em doses que em bovinos que antes nunca ingeriram sementes de R. communis ou A. precatorius causaram intoxica o de intensidade leve a acentuada. Estes resultados permitem concluir que bovinos imunizados contra a a o tóxica das sementes de A. precatorius s o resistentes à a o tóxica das sementes de R. communis, mas que o contrário n o ocorre, isto é, bovinos imunizados contra a a o tóxica das sementes de R. communis, n o se mostraram protegidos contra a intoxica o por A. precatorius. Estudos anteriores por outros autores mostraram que as toxalbuminas de A. precatorius e R. communis, respectivamente abrina e ricina, s o diferentes do ponto de vista antigênico. Uma explica o para a divergência desses resultados com os nossos poderia estar no fato de que no presente estudo foram usados poligástricos que receberam as sementes por via oral, enquanto que nos estudos anteriores foram usados monogástricos em que as sementes ou as toxinas foram aplicadas por via parenteral. A administra o de folhas frescas ou do pericarpo do fruto de R. communis a bovinos imunizados contra a a o das sementes desta planta tiveram o mesmo efeito tóxico que em animais n o imunizados, demonstrando que a imunidade conferida pela ricina n o inibe a a o da ricinina, o principio tóxico das folhas e do pericarpo.
Sobre o "ronca", doen a de etiologia obscura em bovinos, caracterizada por respira o ruidosa
Tokarnia Carlos Hubinger,Dbereiner Jürgen
Pesquisa Veterinária Brasileira , 1998,
Abstract: S o apresentados os resultados de estudos realizados sobre uma doen a de evolu o cr nica em bovinos, de etiologia obscura, cuja caraterística principal é uma respira o ruidosa. Esta doen a é conhecida nos Estados do Piauí, Rio de Janeiro e Mato Grosso do Sul, onde a sua ocorrência foi verificada, pelos nomes populares de "ronca", "roncadeira" ou "ronqueira". Os dados mais importantes da doen a, baseados nos históricos, em observa es próprias, exames clínicos, necropsias de 12 bovinos, complementadas por exames histopatológicos e analises químicas de amostras de fígado para microelementos, s o a respira o ruidosa na inspira o, que aparece ou se intensifica a medida que se lida com os animais, irritabilidade, emagrecimento progressivo e morte após alguns meses até dois anos. Exames de sangue, realizados nos estudos no Piauí, revelaram uma condi o normo-cítica hipocr mica; esfrega os de sangue n o revelaram nada digno de nota. à necropsia se constatou às vezes colora o alaranjada do fígado e colora o alaranjada/marrom dos linfonodos do hilo do fígado. Exames da cavidade nasal, especialmente dos cornetos, e da laringe, n o revelaram nenhuma altera o. Os exames histopatológicos evidenciaram como les o principal hemossiderose acentuada do fígado, sempre presente, bem como do ba o e de linfonodos. Análises de amostras de fígado revelaram em todos os animais afetados pela doen a, nas três regi es, valores muito baixos de cobre e muito elevados de ferro. Dois bovinos afetados pela doen a, transferidos para regi o indene, continuaram com a respira o ruidosa durante mais de um ano. à necropsia destes animais n o foram encontradas les es, além de alguns achados n o relacionáveis com a doen a. Nos exames histopatológicos foi constatada hemosside-rose no fígado, ba o e em linfonodos, porém menos acentuada que nos casos de "ronca" examinados no local de sua procedência. As análises de amostras de fígado revelaram valores normais para cobre e de ferro ainda altos, mas menos acentuados que nos bovinos com "ronca" no local de sua procedência. Os estudos realizados n o permitiram desvendar a raz o da respira o ruidosa. é levantada a suspeita, que a deficiência de cobre tenha influência na patogênese do "ronca", em que, porém, devem estar envolvidos ainda outros fatores para a forma o do quadro.
Bactérias diazotróficas em mudas de bananeira
WEBER, OLMAR BALLER;BALDANI, JOSé IVO;DBEREINER, JOHANNA;
Pesquisa Agropecuária Brasileira , 2000, DOI: 10.1590/S0100-204X2000001100021
Abstract: the objective of this work was to select strains of diazotrophic bacteria associated with banana plants and to determine micropropagated plantlet-growth-promotion by their inoculation with diazotrophs. isolates of herbaspirillum-like and burkholderia cepacia related bacteria were inoculated in plantlets of banana cv. prata an? and the cultivar caipira. plantlets of banana cv. prata an? grown in vitro on a poor nitrogen substrate presented higher fresh weight when herbaspirillum-like bacteria were used as inoculum, while the banana cv. caipira grew better with the inoculation of burkholderia cepacia related bacteria. nevertheless, plants of the cultivar caipira cultivated in plastic bags filled with sand and vermiculite (1:2) and supplied with hoagland's solution containing 5 mg l-1 of n grew faster with the inoculation of herbaspirillum-like and burkholderia cepacia related bacteria. this effect was similar to 50 mg l-1 of n fertilizer added to the positive control. however, the control plants showed higher n content in their shoots when compared to bacterial inoculated plants. this work constitutes the first announcement of banana plant-growth-promotion by diazotrophic bacteria.
Ocorrência de micorrizas arbusculares e da bactéria diazotrófica Acetobacter diazotrophicus em cana-de-a?úcar
Reis, Veronica Massena;Paula, Mauro Augusto de;Dbereiner, Johanna;
Pesquisa Agropecuária Brasileira , 1999, DOI: 10.1590/S0100-204X1999001000021
Abstract: the occurrence and distribution of species of arbuscular mycorrhizae fungi and acetobacter diazotrophicus in sugar cane (saccharum officinarum) grown in different regimes of crop management in the states of rio de janeiro and pernambuco, brazil, were studied. thirty five samples of the rhizosphere soil and roots were collected from 14 varieties of sugar cane for the extraction of spores and isolation of the bacterium. the number of spores varied from 18 to 2,070 per 100 ml of soil, and the greatest diversity of fungal species was found in the sugarcane fields of campos (rio de janeiro state), especially in those where the sugarcane trash was not burned at harvest. the predominant species found in the three localities sampled were: scutellospora heterogama, glomus etunicatum, glomus occultum, acaulospora sp. and gigaspora margarita. a. diazotrophicus was present in almost all samples of root with the exception of one harvest of sugar cane taken from an area used for the sedimentation of vinasse (distillery waste). it was not possible to detect the bacterium from surface sterilized spores of native arbuscular mycorrhizal fungi (amf), only from washed ones using sterile water.
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