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Search Results: 1 - 10 of 1081 matches for " Ayami Kaneko "
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The Sucrose Starvation Signal Mediates Induction of Autophagy- and Amino Acid Catabolism-Related Genes in Cowpea Seedling  [PDF]
Ayami Kaneko, Eri Noguchi, Yushi Ishibashi, Takashi Yuasa, Mari Iwaya-Inoue
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.43083

In higher plants, autophagy is bulk degradation process in vacuole necessary for survival under nutrient-limited conditions and plays important roles in senescence, development and pathogenic response, etc. Cowpea is one of the most important legume crops in semi-aride region, which is highly tolerant to drought stress. Changes of photoassimilate status by drought stress and/or sink-source balance appeared to affect autophagy and senescence of leaf in cowpea. Accordingly, we focused on roles of sucrose signal in autophagy and amino acid recycling in cowpea. Effects of starvation stress on the expression of autophagy-related genes (ATGs) and amino acid catabolism-related genes in cowpea [Vigna unguiculata (L.) Walp] were examined by Reverse transcription-polymerase chain reaction (RT-PCR) and anti-ATG8i specific antibody. Sucrose starvation stress enhanced the expression levels of VuATG8i, VuATG8c and VuATG4 incowpea seedlings. The expressions of amino acid catabolism related genes, such as asparagine synthase (VuASN1), proline dehydrogenase1 (VuProDH) and branched chain amino acid transaminase (VuBCAT2), are also up-regulated under the sucrose starvation. In contrast, high sucrose condition suppressed autophagy and the expressions of ATGs. These results indicate that sucrose starvation stress stimulates both autophagy and amino acid catabolism by regulation of ATGs and VuBCAT2. It is conceivable that sucrose starvation stress enhances autophagy in cowpea, possibly via branched chain amino acid level regulated by the starvation-induced BCAT.

Wrinkled 1 (WRI1) Homologs, AP2-Type Transcription Factors Involving Master Regulation of Seed Storage Oil Synthesis in Castor Bean (Ricinus communisL.)  [PDF]
Daichi Tajima, Ayami Kaneko, Masatsugu Sakamoto, Yumena Ito, Nong Thi Hue, Masayuki Miyazaki, Yushi Ishibashi, Takashi Yuasa, Mari Iwaya-Inoue
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.42044
Abstract: Among APETALA2 (AP2)-type plant specific transcription factor family, WRINKLED1 (WRI1), has appeared to be a master gene transcriptionally regulating a set of carbon metabolism- and fatty acid synthesis (FAS)-related genes responsible for seed specific triacylglycerols (TAGs) storage in oil plants. B3 type transcription factors, such as ABI3 and FUS3, are known to be involved in seed development, such as seed storage protein synthesis and maturation. Based on the recent whole genome sequence data of castor bean (Ricinus communis L.), putative WRI1 homologs (RcWRI1, RcWRI2) specifically expressed in castor bean seed have been identified by comparing organ specific expression profiles among seed development-related transcription factors, seed storage specific genes (Ricin, RcOleosin) and a set of FAS genes including genes for sucrose synthase (RcSUS2), biotin carboxyl carrier protein (a subunit of acetyl-CoA carboxylase, RcBCCP2) and ketoacyl-acyl carrier protein synthase (RcKAS1). Immunoreactive signals with WRI1, FUS3 and ABI5-related polypeptides were also detected in seed specifically, consistent with the expression profiles of seed development-related genes. The WRI1 binding consensus sites, [CnTnG](n)(7)[CG], designated as the AW-box, were found at the promoter region of RcBCCP2 and RcKAS1. Thus, RcWRI1 possibly play a pivotal role in seed specific TAGs storage during seed development by directly activating FAS -related genes.
Differences in Root Growth and Permeability in the Grafted Combinations of Dutch Tomato Cultivars (Starbuck and Maxifort) and Japanese Cultivars (Reiyo, Receive, and Magnet)  [PDF]
Tomomi Kakita, Ayami Abe, Takashi Ikeda
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.616266
Abstract: Grafting is widely established in agriculture and provides practical advantages for vegetable production. We investigated physiological differences between the grafted combinations of Dutch (Starbuck and Maxifort) and Japanese (Reiyo, Receive and Magnet) tomato cultivars. Plants were grown hydroponically until the flowers on the first truss bloomed, and the following parameters were measured: fresh weight of the aerial parts, root surface area, root permeability (by using a pressure chamber), and water potential of exudates (by using an isopiestic psychrometer). The Starbuck/Maxifort combination had higher values of the aerial part weight, root surface area, and root permeability than Reiyo/Receive, whereas Reiyo/Maxifort tended to have higher values of these parameters than Reiyo/Receive and Reiyo/Magnet. Maxifort had a significantly larger root surface area than Receive, but root permeability was not significantly different. InReiyo/Maxifort and Starbuck/Receive, these parameters were not significantly different except for a single comparison of root permeability. Thus, root permeability and root surface area may depend not only on the rootstock but be also affected by scion in grafted plants. Water potential of exudates was similar in most combinations and experiments. This shows that three rootstock cultivars provided similar nutrient concentrations even with different scions.
Induction of a bZIP Type Transcription Factor and Amino Acid Catabolism-Related Genes in Soybean Seedling in Response to Starvation Stress
Takashi Yuasa,Yuri Nagasawa,Katsumasa Osanai,Ayami Kaneko,Daichi Tajima,Nang Myint Phyu Sin Htwe,Yushi Ishibashi,Mari Iwaya-Inoue
Journal of Botany , 2013, DOI: 10.1155/2013/935479
Abstract: To address roles of bZIP transcription factors on regulation of amino acid catabolism under autophagy-induced plant cells, we examined the effect of nutrient starvation on the expression of low energy stress-related transcription factor homologs, GmbZIP53A and GmbZIP53B, and amino acid catabolism-related genes in soybean (Glycine max (L.) Merr.). Sucrose starvation treatment significantly enhanced the expressions of GmbZIP53A, but not GmbZIP53B asparagine synthase (GmASN1), proline dehydrogenase1 (GmProDH), and branched chain amino acid transaminase 3 (GmBCAT3). GmbZIP53-related immunoreactive signals were upregulated under severe starvation with sucrose starvation and protease inhibitors, while 3% sucrose and sucrose starvation had no or marginal effects on the signal. Profiles of induction of GmASN1, GmProDH and GmBCAT3 under various nutrient conditions were consistent with the profiles of GmbZIP53 protein levels but not with those of GmbZIP mRNA levels. These results indicate that GmbZIP53 proteins levels are regulated by posttranslational mechanism in response to severe starvation stress and that the increased protein of GmbZIP53 under severe starvation accelerates transcriptional induction of GmASN1, GmProDH, and GmBCAT3. Furthermore, it is conceivable that decrease of branched chain amino acid level by the BCAT-mediated degradation eventually enhances autophagy under severe starvation. 1. Introduction The perception and management of nutrient and energy levels in organisms are crucial for survival by adjusting metabolism to available resources. Recent studies revealed that sugar signals in higher plants activate various biological modules such as sugar sensor, transcription factors, sugar transporters, and metabolic enzymes of sugar and amino acids [1, 2]. In higher plants, sugar deprivation and/or low energy stress by decreased photosynthesis have appeared to induce protein degradation via autophagy and amino acid metabolism, leading to translocation of nutrients and senescence [3, 4]. Recently accumulated studies on bZIP type transcription factors of Arabidopsis concerning nutrient signal and amino acid metabolism have unveiled that a set of bZIP transcription factors, bZIP1 and bZIP53, classified to S-type subgroup among the bZIP superfamily, are master regulatory components in transcriptional induction of amino acid catabolism-related enzymes involved in low energy stress, sucrose starvation, and senescence-induced nutrient translocation [5–7]. Sucrose starvation and/or low energy stress activate bZIP1 and bZIP53a by transcriptional and
Exploring New Socio-Economic Thoughts for a Small and Narrow Earth  [PDF]
Mamoru Kaneko
Advances in Applied Sociology (AASoci) , 2018, DOI: 10.4236/aasoci.2018.85024
Abstract: The earth has been getting smaller and narrower with the expansion of human activities. Now, it is an urgent task for social scientists to explore and study new socio-economic thoughts for this small and narrow earth. This article provides a normative theory and a descriptive theory for the present and future earth. The former is to provide, viewing the earth and human community as unity, evaluations of possible events and of designs of social institutions. The normative theory helps us think about where we should direct the earth. The latter discusses social sciences for practical management of the earth. Since, however, great diversity of cultures will remain, a unified management of the earth is practically impossible. We are required to rethink and develop new socio-economic thoughts in radical manners.
Effects of the inhibition of weed communities by winter-flooding  [PDF]
Korehisa Kaneko, Toshihiko Nakamura
Agricultural Sciences (AS) , 2011, DOI: 10.4236/as.2011.24050
Abstract: Winter-flooded paddy field is an agricultural method which putting a water among the winter, it is paid attention as an environmental friendly agriculture. Especially, it is said, winter-flooding is control paddy weeds and there is the farming potential that the annual rice yield is high. However, there is no detailed research about the effects of weed communities by winter-flooding, as the purpose of this study, we investigated the effects of the inhibition of weed communities (life cycle, harmful weed) by winter-flooding at a shore of Inba Lake, Chiba Prefecture, Japan, and compared them to paddy weeds in a dry paddy field. Methods examined the plant height (cm) and the cover degree class of all appearance species in each quadrant frame (1 m2). Quadrant in the two type paddies were 10 frames (D1-D10) at the control, and were 10 frames (W1-W10) at the treatment (Figure 1). In a winter-flooded paddy field, species number and plant volume of winter-annual plants decreased in the third winter after winter-flooding, In particular, the plant volume of Alopecurus aequalis, Cardamine flexuosa decreased significantly. We considered that the cause of this decrease was due to the depth of flooding (more than 10 cm). Eleocharis kuroguwai and Echinochloa oryzoides, which are perennial plants, Sagittaria trifolia, which is an annual plant, increased in a winter-flooded paddy field in the third summer after winter-flooding. We considered that these species grew thicker at places where the seeds germinated, and grew easily, because the conservation situation of the seeds fitted well under the winter-flooding conditions. They started to grow immediately after the drainage of water. The period of drainage coincided with increasing light intensity and temperature. In addition, the annual rice yield of the third year after winter-flooding was higher than that of the habitual practice rice field.
Effects of environmental factors on Sparganium emersum and Sparganium erectum colonization in two drainage ditches with different maintenance  [PDF]
Korehisa Kaneko, Hiroshi Jinguji
Agricultural Sciences (AS) , 2012, DOI: 10.4236/as.2012.34064
Abstract: In the Niheishimizu and Ooshimizu sections of the town of Misato in the Akita Prefecture, Northern Japan, there are many abundant spring water areas. Sparganium (Sparganium emersum and Sparganium erectum) species are widely distributed in the irrigation water that fed by spring water. The irrigation waters were divided the natural type ditch and the maintained ditch that connect with nearby natural ditch to promote environmentally friendly agriculture. This study was conducted in both sections to support the maintenance of the irrigation water fed by the abundant spring water. A vegetation survey was conducted in September of 2005. The survey collected data on the amount of vegetation cover and the stem lengths of the plant species found in selected locations of the study area. The water depths and the flow velocities were also measured in these locations. As for the growth situation of S. emersum and S. erectum, the submerged form of S. emersum was found in water approximately 15 cm deep with a surface flow velocity of approximately 7 cm/s. This species was characterised by a relatively fast flow and relatively shallow water. The emergent and submerged growth forms of S. emersum were found in waters having flow velocities faster than those associated with S. erectum. The emergent form of S. emersum grew in relatively deep water. S. emersum is more capable of adjusting to the conditions of stream habitats than S. erectum.
The Psychometric Properties and Factor Structure of the Postpartum Bonding Questionnaire in Japanese Mothers  [PDF]
Hitoshi Kaneko, Shuji Honjo
Psychology (PSYCH) , 2014, DOI: 10.4236/psych.2014.59126

The aim of this study was to examine the psychometric properties of the Postpartum Bonding Questionnaire (PBQ) in Japanese mothers. Additionally, we investigated the association between postpartum bonding and postnatal depression. The participants of this study were 1786 Japanese mothers, recruited during their infants’ 3 months check-ups at a public health center. The mothers completed the PBQ and the Edinburgh Postnatal Depression Scale (EPDS). We examined the factor structure of the PBQ. However, we were unable to confirm the original 4 factors. A 1-factor solution was extracted by omitting 9 items that did not have significant loadings onto the single factor. According to the cut-off values of the original PBQ, the positive rate was 7.7%. The PBQ score was moderately correlated with the EPDS score. The abridged 16-item Japanese version of the PBQ can be used as a reliable screening tool during check-ups for Japanese mothers. Health practitioners should pay attention to mothers who have bonding disturbances at postpartum.

Review of Effective Vegetation Mapping Using the UAV (Unmanned Aerial Vehicle) Method  [PDF]
Korehisa Kaneko, Seiich Nohara
Journal of Geographic Information System (JGIS) , 2014, DOI: 10.4236/jgis.2014.66060
Abstract: We tried more precise mapping of vegetation using UAV?(unmanned aerial vehicle), as a new method of creating vegetation maps, and we?objected to be clearly the efficient mapping of vegetation using the UAV method by comparing vegetation maps created by analysing aerial photographs taken by a UAV and an aircraft (manned flight). The aerial photography using UAV was conducted in the Niida River estuary (the secondary river flowing into Minamisoma City in Fukushima Prefecture, Japan). The photography period was in August 2013. We analysed the aerial photographs using ArcGis 9 (Esri Japan Corporation, Tokyo, Japan). The aerial photographs of the main plant communities (Phragmites australis,?Typha domingensis, and?Miscanthus sacchariflorus) taken by the UAV could clearly discriminate each plant community at the 1/50 scale. Moreover, it could clearly discriminate the shape of a plant at the 1/10 scale. We compared the vegetation maps by analysing the aerial photos taken by a UAV (2013 shooting) and an aircraft (2011 shooting). As a result, the vegetation map created by the UAV method could clearly discriminate community distributions. We conclude that vegetation surveys using UAV are possible and are capable of a highly precise community division in places where field reconnaissance is difficult. The UAV method is effective and will contribute to the improvement of research methods in the future; this method may reduce research costs associated with a reduction in field survey days and man-power.
The influence of changes in the degree and frequency of disturbance on the annual salt marsh plant (Suaeda maritima, Artemisia fukudo) communities in estuarine tidal flats: A case study of the Kushida River in Mie Prefecture, Japan  [PDF]
Korehisa Kaneko, Seiich Nohara
Open Journal of Ecology (OJE) , 2014, DOI: 10.4236/oje.2014.41001
Abstract: In this study, we examined the influence of changes in the degree and frequency of disturbance in estuarine tidal flats on the annual salt marsh plant communities (Suaeda maritima, Artemisia fukudo) in Mie Prefecture, Japan. Suaeda maritima and Artemisia fukudo communities occur in the branch river of the Kushida River. Although the areas occupied by these communities were very small in 2006, the Suaeda maritima community expanded significantly to 3609 m2 in 2008, and the Artemisia fukudo community expanded significantly to 2726 m2 in 2008 and 10,396 m2 in 2010. Before the onset of the investigation period in 2006, the overflow warning water level (3.5 m) and the flood fighting corps standby water level (3.0 m) each occurred on one day in August 2004 and October 2004, respectively; at those times, the water volume exceeded 1000 m3·s-1 and 1500 m3·s-1, respectively. We suggest that because much of the estuarine tidal flat erodes when the water volume exceeds 1000 m3·s-1, the establishment of the Suaeda maritima and Artemisia fukudo communities is delayed until sufficient substrate is formed by the deposition of new sediment. In contrast, a water level of 2 - 3 m was observed on one day each in 2005, 2007 and 2009, with average water volumes of 488.5, 566.4 and 690.1 m3·s-1, respectively. We suggest that following the repeated disturbances caused by water levels of 1 - 3 m and flow volumes of 500 - 700 m3·s
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