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Biomarkers responses in fish (Atherinella brasiliensis) of paranaguá bay, southern Brazil, for assessment of pollutant effects
Ciro Alberto de Oliveira Ribeiro,Alberto Katsumiti,Patrícia Fran?a,Jocieli Maschio
Brazilian Journal of Oceanography , 2013,
Abstract: Paranaguá bay is a complex estuary located in southern Brazil containing three protected areas listed by UNESCO. Historically, the estuary has been affected by urban, industrial, agricultural and harbor activities, and occasional accidents. Specifically, the explosion of the Chilean ship Vicu a in December 2004 spilled methanol and crude and fuel oils which affected both protected and non-protected areas. The present study sought to investigate the pollution threat to aquatic organisms in order to evaluate the potential effects of pollutants. One hundred and twenty adult fish Atherinella brasiliensis were collected from different sites within Paranaguá estuary, including the harbor and open ocean, during summer, autumn and winter of 2005. Among the biomarkers, the somatic index, chemical analysis of bile, biochemical, genetic and morphological parameters were considered. Chemical analysis of bile showed a continuous bioavailability of polycyclic aromatic hydrocarbons (PAHs) according to proximity to the harbor site. The histopathological findings have demonstrated aconsiderable incidence of severe pathologies in the liver and gills, corroborated by biochemical disturbances and genetic damage. These findings indicate that more studies are necessary to evaluate both water quality and fish health so as to permit a better analysis of the impact of pollution in Paranaguá estuary. A Baia de Paranaguá é um complexo estuarino localizado no sul do Brasil constituído de três áreas de prote o ambiental listadas pela UNESCO. Historicamente, o estuário tem sido afetado por atividade urbana, industrial, agricultura e portuária, e eventualmente por acidentes. Particularmente a explos o do navio Chileno Vicu a em dezembro de 2004 derramou metanol, óleo cru e combustível atingindo áreas protegidas e n o protegidas. O presente estudo tem por objetivo investigar a polui o em organismos aquáticos. Cento e vinte indivíduos adultos do peixe Atherinella brasiliensis foram coletados em quatro diferentes pontos de coleta no estuário de Paranaguá, partindo do porto até o oceano aberto nos períodos de ver o, inverno e primavera de 2005. Os índices somáticos, parametros químicos, enzimáticos, genéticos e morfológicos foram considerados. As análises histopatológicas demonstraram expressiva incidência de patologia no fígado e nas branquias algumas vezes corroboradas pelas altera es bioquímicas. Danos genéticos e anormalidades genéticas também foram observados. As análises químicas na bile mostraram uma contínua biodisponibilidade de hidrocarbonetos policíclicos aromáticos para os
Micetomas actinomicóticos no Rio Grande do Sul: relato de quatro casos
Londero, Alberto T.;Ramos, Cecy D.;Matte, Shirley W.;
Memórias do Instituto Oswaldo Cruz , 1986, DOI: 10.1590/S0074-02761986000100010
Abstract: four cases of mycetoma caused by n. brasiliensis, occurring in rio grande do sul (brazil), are reported.
Nuttallia brasiliensis e Theileria brasiliensis, sinonímias de Babesia brasiliensis (Piroplasmida: Babesiidae) hemoparasito de marsupiais Didelphidae
Parasitología latinoamericana , 2003, DOI: 10.4067/S0717-77122003000100018
Abstract: transference of the south american opossums hemoparasite nuttallia brasiliensis to the genus babesia are discussed and proposed due to the pre-occupation of nuttallia genus by mollusk species, as well as the synonym of theileria brasiliensis to babesia brasiliensis. simultaneously the situation of the others members of nuttallia and achromaticus genus is discussed, in relation with the international rules of nomenclature and its common biologic and morphologic aspects. also a chronological summary of b. brasiliensis reports is given
Nuttallia brasiliensis e Theileria brasiliensis, sinonímias de Babesia brasiliensis (Piroplasmida: Babesiidae) hemoparasito de marsupiais Didelphidae Nuttallia brasiliensis AND Theileria brasiliensis, SYNONYM OF Babesia brasiliensis (PIROPLASMIDA: BABESIIDAE) HEMOPARASITE OF MARSUPIALS
Parasitología latinoamericana , 2003,
Abstract: é discutida a posi o taxon mica do hemoparasito de marsupiais sul-americanos, descrito como Nuttallia brasiliensis, propondo-se a sua transferência ao gênero Babesia devido à préocupa o do gênero Nuttallia por espécies de moluscos, propondo-se também a sinonímia de Theileria brasiliensis em rela o a Babesia brasiliensis. Simultaneamente é discutida a situa o de representantes de outros gêneros como Nuttallia e Achromaticus, em rela o às normas internacionais de nomenclatura, e a seus aspectos morfológicos e biológicos comuns. Também é apresentado um sumário cronológico dos relatos de B. brasiliensis Transference of the South American opossums hemoparasite Nuttallia brasiliensis to the genus Babesia are discussed and proposed due to the pre-occupation of Nuttallia genus by mollusk species, as well as the synonym of Theileria brasiliensis to Babesia brasiliensis. Simultaneously the situation of the others members of Nuttallia and Achromaticus genus is discussed, in relation with the international rules of nomenclature and its common biologic and morphologic aspects. Also a chronological summary of B. brasiliensis reports is given
Paracoccidioidoma medular
Betancur,Carlos Alberto; Ruiz,Carlos;
Acta Medica Colombiana , 2010,
Abstract: the case is reported of a 51-year-old patient with a three-month history of paraparesia that had rendered him unable to walk over a period of 45 days. the patient underwent surgical excision of 2 granulomas that had been shown by an mri scan. histopathological study with silver methenamine showed budding blastoconidia, typical of paraccoccidioides brasiliensis infection (acta med colomb 2010; 35: 183-184).
Avalia??o do efeito antiparasitário do omeprazol na preven??o do desenvolvimento de les?es cutaneas em hamsters infectados por Leishmania brasiliensis
Miot, Hélio Amante;Miot, Luciane Donida Bartoli;Costa, Ana Laura Bastos da;Matsuo, Cristiane Yuri;O?Dwyer, Lúcia Helena;
Anais Brasileiros de Dermatologia , 2005, DOI: 10.1590/S0365-05962005001000011
Abstract: background: cutaneous leishmaniasis remains an endemic disease in several brazilian regions. the parasite? survival in macrophages is due to a membrane k+/h+-atpase that can be inhibited by omeprazole. objectives: evaluate omeprazole?s efficacy preventing the development of cutaneous leishmaniasis in hamsters. methods: eighteen hamsters were divided in 3 groups: the l group received an inoculation of l. brasiliensis on right paw, the o group received daily 0,4mg omeprazole subcutaneously, and l+o group received both omeprazole and the inocule. the study was performed in 42 days, and the measurements of the diameter of paws were done weekly. at the end of the study was carried out a smear to verify the presence of parasites. results: hamsters fitted in l and l+o groups have developed cutaneous leishmaniasis? lesions, there were ulcerations in 2 hamsters from l group and 1 from l+o group. at the end of the study, the vitality and mobility of animals from l group were less prominent than l+o and o groups. diameters of inoculated paws in l and l+o groups were significantly larger in comparison to begin of the study (p<0,05). there were no statistical difference between diameters of the paws from l and l+o groups at the end of study (p0,05). parasites were detected at microscopic smears of lesions from both groups. conclusions: hamsters? cutaneous lesions due to leishmania sp. inoculation couldn?t be prevented by omeprazole, using this protocol.
Optimization of a mouse immunization protocol with Paracoccidioides brasiliensis antigens
Almeida, Armando Vieira;Foga?a, Joel;Chamma, Luiz Gast?o;Fecchio, Denise;Franco, Marcello;
Memórias do Instituto Oswaldo Cruz , 1995, DOI: 10.1590/S0074-02761995000100011
Abstract: the objectives of the present study were to optimize the protocol of mouse immunization with paracoccidioides brasiliensis antigens (rifkind's protocol) and to test the modulation effect of cyclophosphamide (cy) on the delayed hypersensitivity response (dhr) of immunized animals. experiments were carried out using one to four immunizing doses of either crude particulate p. brasiliensis antigen or yeast-cell antigen, followed by dhr test four or seven days after the last immunizing dose. the data demonstrated that an immunizing dose already elicited response; higher dhr indices were obtained with two or three immunizing doses; there were no differences between dhr indices of animals challenged four or seven days after the last dose. overall the inoculation of two or three doses of the yeast-cell antigen, which is easier to prepare, and dhr test at day 4 simplify the original rifkind's immunization protocol and shorten the duration of the experiments. the modulation effect of cy on dhr was assayed with administration of 2.5, 20 and 100 mg/kg weight at seven day intervals starting from day 4 prior to the first immunizing dose. only the treatment with 2.5 mg cy increased the dhr indices. treatment with 100 mg cy inhibited the dhr, whereas 20 mg cy did not affect the dhr indices. results suggest an immunostimulating effect of low dose of cy on the dhr of mice immunized with p. brasiliensis antigens.
Saponinas triterpênicas de Tocoyena brasiliensis Mart. (Rubiaceae)
Hamerski, Lidilhone;Carbonezi, Carlos Alberto;Cavalheiro, Alberto José;Bolzani, Vanderlan da Silva;Young, Maria Cláudia Marx;
Química Nova , 2005, DOI: 10.1590/S0100-40422005000400009
Abstract: the present communication reports the isolation and identification of four triterpenoid saponins from the chloroform extract of the leaves of tocoyena brasiliensis: 3-o-b-d-quinovopyranosyl quinovic acid, 3-o-b-d-quinovopyranosyl cincholic acid, 3-o-b-d-glucopyranosyl quinovic acid and the 28-o-b-d-glucopyranosyl ester derivative of quinovic acid as binary mixtures, respectively. from the ethanol extract a flavonoid identified as ramnazin-3-o-rutinoside was obtained. the structures of these compounds were assigned by data analysis of 1d and 2d nmr spectrometry and comparison with data recorded in the literature for these compounds.
Inmunoglobulinas en pacientes con actinomicetoma por Nocardia brasiliensis
Méndez-Tovar,L. J.; Mondragón-González,R.; Manzano-Gayosso,P.; López-Martínez,R.; Hernández-Hernández,F.; Bonifaz,A.; Anides Fonseca,A.; Araiza,J.; Vega-López,F.;
Revista argentina de microbiolog?-a , 2004,
Abstract: considering that some authors have reported an increasing of some immunoglobulins in actinomycetoma patients, in this study we propose to determine differential production of igg1, igg2, igg3, igg4 and iggm in 25 patients with actinomycetoma and 25 healthy individuals from a mycetoma endemic area. immunoglobulins were determined by elisa technique. to sensibilize the plates, six nocardia brasiliensis antigens were used: a crude antigen denominated nb and five derivatives (nb2, nb4, nb6, nb8 and nb10) obtained by their isoelectric point. results showed that all igg subclasses were higher in the patients’ sera than in control sera, with a maximal difference to igg3 and igg4. to the latter subclass, six antigens were highly reactives. igm levels were similar in both groups. as it occurs in other infections, in the actinomycetoma pathogenesis probably participate the increase or deficiency of a determined immunoglobulin class, as well as the relationship between different subclasses.
Septicemia caused by Paracoccidioides brasiliensis (Lutz, 1908) as the cause of death of an aids patient from Santos, S?o Paulo State, Brazil ? a nonendemic area
Caseiro, Marcos Montani;Etzel, Arnaldo;Soares, Maria Cecilia B.;Costa, Sergio Olavo Pinto da;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2005, DOI: 10.1590/S0036-46652005000400006
Abstract: the first case of paracoccidioides brasiliensis in santos (brazil) leading to septicemia and death of an hiv-positive patient is reported here. the patient was a 34-year-old female that presented essential fever and was only diagnosed after death by positive blood culture. the authors underscore the atypical nature of the case, since the patient was a female at fertile age who was born and had always lived in santos, which is a nonendemic area for this infection.
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