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Search Results: 1 - 10 of 4959 matches for " Aroa Ejarque-Ortiz "
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Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
Guido Dentesano, Marco Straccia, Aroa Ejarque-Ortiz, Josep M Tuselll, Joan Serratosa, Josep Saura, Carme Solà
Journal of Neuroinflammation , 2012, DOI: 10.1186/1742-2094-9-165
Abstract: Murine primary microglial cultures, mixed glial cultures from wild-type and CCAAT/enhancer binding protein β (C/EBPβ)-deficient mice, and the BV2 murine cell line overexpressing C/EBPβ were used to study the involvement of C/EBPβ transcription factor in the regulation of CD200R1 expression in response to a proinflammatory stimulus (lipopolysaccharide (LPS)). Binding of C/EBPβ to the CD200R1 promoter was determined by quantitative chromatin immunoprecipitation (qChIP). The involvement of histone deacetylase 1 in the control of CD200R1 expression by C/EBPβ was also determined by co-immunoprecipitation and qChIP.LPS treatment induced a decrease in CD200R1 mRNA and protein expression in microglial cells, an effect that was not observed in the absence of C/EBPβ. C/EBPβ overexpression in BV2 cells resulted in a decrease in basal CD200R1 mRNA and protein expression. In addition, C/EBPβ binding to the CD200R1 promoter was observed in LPS-treated but not in control glial cells, and also in control BV2 cells overexpressing C/EBPβ. Finally, we observed that histone deacetylase 1 co-immunoprecipitated with C/EBPβ and showed binding to a C/EBPβ consensus sequence of the CD200R1 promoter in LPS-treated glial cells. Moreover, histone deacetylase 1 inhibitors reversed the decrease in CD200R1 expression induced by LPS treatment.CD200R1 expression decreases in microglial cells in the presence of a pro-inflammatory stimulus, an effect that is regulated, at least in part, by C/EBPβ. Histone deacetylase 1 may mediate C/EBPβ inhibition of CD200R1 expression, through a direct effect on C/EBPβ transcriptional activity and/or on chromatin structure.
Pro-inflammatory gene expression and neurotoxic effects of activated microglia are attenuated by absence of CCAAT/enhancer binding protein β
Marco Straccia, Núria Gresa-Arribas, Guido Dentesano, Aroa Ejarque-Ortiz, Josep M Tusell, Joan Serratosa, Carme Solà, Josep Saura
Journal of Neuroinflammation , 2011, DOI: 10.1186/1742-2094-8-156
Abstract: Due to fertility and mortality problems associated with the C/EBPβ-null genotype we developed a protocol to prepare mixed glial cultures from cerebral cortex of a single mouse embryo with high yield. Wild-type and C/EBPβ-null glial cultures were compared in terms of total cell density by Hoechst-33258 staining; microglial content by CD11b immunocytochemistry; astroglial content by GFAP western blot; gene expression by quantitative real-time PCR, western blot, immunocytochemistry and Griess reaction; and microglial neurotoxicity by estimating MAP2 content in neuronal/microglial cocultures. C/EBPβ DNA binding activity was evaluated by electrophoretic mobility shift assay and quantitative chromatin immunoprecipitation.C/EBPβ mRNA and protein levels, as well as DNA binding, were increased in glial cultures by treatment with lipopolysaccharide (LPS) or LPS + interferon γ (IFNγ). Quantitative chromatin immunoprecipitation showed binding of C/EBPβ to pro-inflammatory gene promoters in glial activation in a stimulus- and gene-dependent manner. In agreement with these results, LPS and LPS+IFNγ induced different transcriptional patterns between pro-inflammatory cytokines and NO synthase-2 genes. Furthermore, the expressions of IL-1β and NO synthase-2, and consequent NO production, were reduced in the absence of C/EBPβ. In addition, neurotoxicity elicited by LPS+IFNγ-treated microglia co-cultured with neurons was completely abolished by the absence of C/EBPβ in microglia.These findings show involvement of C/EBPβ in the regulation of pro-inflammatory gene expression in glial activation, and demonstrate for the first time a key role for C/EBPβ in the induction of neurotoxic effects by activated microglia.Glial activation is an inflammatory process that occurs in astrocytes and microglia to re-establish homeostasis of the CNS after a disequilibrium of normal physiology. Microglia are tissue-associated macrophages that keep the CNS under dynamic surveillance. Most insults to the C
Technical Note: Dissolved organic matter fluorescence – a finite mixture approach to deconvolve excitation-emission matrices
A. Butturini,E. Ejarque
Biogeosciences Discussions , 2013, DOI: 10.5194/bgd-10-4711-2013
Abstract: The analysis of the shape of excitation-emission matrices (EEMs) is a relevant tool for exploring the origin, transport and fate of dissolved organic matter (DOM) in aquatic ecosystems. Within this context, the decomposition of EEMs is acquiring a notable relevance. A simple mathematical algorithm that automatically deconvolves single EEM is described, creating new possibilities for the comparison of DOM fluorescence properties and EEMs that are very different from each other. A mixture model approach is adopted to decompose complex surfaces into sub-peaks. The laplacian operator and the Nelder–Mead optimization algorithm are implemented to individuate and automatically locate potential peaks in the EEM landscape. A small heterogeneous data set of 21 EEMs from a human-impacted Mediterranean river is used to describe the model application and to illustrate a strategy that optimises the search for the optimal output.
Luis E. ALONSO, La era del consumo
Montes Ceballos, Aroa
Revista Internacional de Sociologia , 2008,
Self-Organising Maps and Correlation Analysis as a Tool to Explore Patterns in Excitation-Emission Matrix Data Sets and to Discriminate Dissolved Organic Matter Fluorescence Components
Elisabet Ejarque-Gonzalez, Andrea Butturini
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0099618
Abstract: Dissolved organic matter (DOM) is a complex mixture of organic compounds, ubiquitous in marine and freshwater systems. Fluorescence spectroscopy, by means of Excitation-Emission Matrices (EEM), has become an indispensable tool to study DOM sources, transport and fate in aquatic ecosystems. However the statistical treatment of large and heterogeneous EEM data sets still represents an important challenge for biogeochemists. Recently, Self-Organising Maps (SOM) has been proposed as a tool to explore patterns in large EEM data sets. SOM is a pattern recognition method which clusterizes and reduces the dimensionality of input EEMs without relying on any assumption about the data structure. In this paper, we show how SOM, coupled with a correlation analysis of the component planes, can be used both to explore patterns among samples, as well as to identify individual fluorescence components. We analysed a large and heterogeneous EEM data set, including samples from a river catchment collected under a range of hydrological conditions, along a 60-km downstream gradient, and under the influence of different degrees of anthropogenic impact. According to our results, chemical industry effluents appeared to have unique and distinctive spectral characteristics. On the other hand, river samples collected under flash flood conditions showed homogeneous EEM shapes. The correlation analysis of the component planes suggested the presence of four fluorescence components, consistent with DOM components previously described in the literature. A remarkable strength of this methodology was that outlier samples appeared naturally integrated in the analysis. We conclude that SOM coupled with a correlation analysis procedure is a promising tool for studying large and heterogeneous EEM data sets.
Classification, Identification, and Manipulation of Relevant Factors for Adaptation and Behavioural Adjustment from a Psychological Point of View  [PDF]
Gerardo Ortiz
Psychology (PSYCH) , 2014, DOI: 10.4236/psych.2014.513162
Abstract: Generally, the study of animal welfare is based on the identification and promotion of speciestypical behaviors of the individual or target group. The adjustment to new conditions (i.e. captivity) is easier for some species, while for others it may be very difficult or even impossible. The adjustment to captive conditions is a basic element for the development of conservation strategies (i.e. translocation, introduction, and reintroduction) and can be measured by different variables related to an animal’s psychological well-being. From a psychological point of view, we assume that organisms can adjust their behavior in correspondence to changes in their environment, adjustment that is enabled by an ecological contact medium (e.g. Ribes, 2007; Ribes & Perez-Almonacid, 2011). Under this assumption, we propose a methodology that allows the classification, identification and manipulation of relevant factors for an individual’s adjustment to different conditions (i.e. freedom and captivity) and a more rational handling of organisms and their specific life condition. The main elements of this methodology are: 1) adaptive and survival circumstances; 2) description of ecological milieu; 3) interactive processes (i.e. intra-individual, inter-individual, and inter-individual dependence); and 4) interaction-situation relationship.
Be?at Artetxe,Leire San Felices,Aroa Pache,Santiago Reinoso
Acta Crystallographica Section E , 2013, DOI: 10.1107/s1600536813000330
Abstract: The title compound, [Co(C4H3N2O2)2(H2O)2], contains a CoII cation on a twofold rotation axis, exhibiting a distorted octahedral coordination geometry. The equatorial plane is formed by two N,O-bidentate 1H-imidazole-4-carboxylate ligands and the axial positions are occupied by water molecules. The crystal packing consists of a three-dimensional network stabilized by O—H...O and N—H...O hydrogen bonds, together with weak π–π interactions [centroid–centroid distance = 3.577 (2) ] between the imidazole rings.
Tetrakis(μ2-ferrocenecarboxylato-κ2O:O′)bis[(methanol-κO)copper(II)] methanol disolvate
Beñat Artetxe,Pablo Vitoria,Aroa Pache,Santiago Reinoso
Acta Crystallographica Section E , 2011, DOI: 10.1107/s1600536811050185
Abstract: The complex molecule of the title compound, [Cu2Fe4(C5H5)4(C6H4O2)4(CH3OH)2]·2CH3OH, lies about an inversion centre and contains two centrosymetrically related CuII atoms bridged by four O:O′-bidentante ferrocenecarboxylate anions, leading to a dimeric tetrabridged unit with a paddle-wheel geometry. The CuII atom has a distorted square-pyramidal coordination environment with four O atoms from four ferrocenecarboxylate ligands in basal positions and an O atom from a methanol molecule in an apical position. One of the two crystallographically independent ferrocenyl groups has a staggered conformation, while the other is eclipsed. The molecules are connected into a chain along the b axis by O—H...O hydrogen bonds involving coordinated and uncoordinated methanol molecules and the O atom from a ferrocenecarboxylate unit.
Molecular characterisation of virulence graded field isolates of myxoma virus
Kevin P Dalton, Ines Nicieza, Aroa Baraga?o, Jose Alonso, Francisco Parra
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-49
Abstract: The findings reported here show the analysis of 16 genomic regions accounting for approximately 10% of the viral genomes. Of the 20 genes analysed 5 (M034L, M069L, M071L, M130R and M135R) were identical in all strains and 1 (M122R) contained only a single point mutation in an individual strain. Four genes (M002L/R, M009L, M036L and M017L) showed insertions or deletions that led to disruption of the ORFs.The findings presented here provide valuable tools for strain differentiation and phylogenetic studies of MV isolates and some clues as to the reasons for virus attenuation in the field.Myxoma virus (MV) causes myxomatosis in the European rabbit (Oryctolagus cuniculus). The disease is a recurrent problem in rabbit farms and in wild rabbit populations throughout Europe [1-4]. Due to the unique manner of its introduction into the European rabbit population, MV provides an excellent model for studying the coevolution of a virus and its host. The complex virus/host relationship has been shown to lead to the emergence of more transmissible/attenuated virus strains and more resistant hosts (for review see [5]). Such studies have been carried out using experimental rabbit infections, however, few studies have characterised the sequence changes incurred by the virus during adaptation to its new host [1,6-10].MV strains are classified into 5 virulence grades (I to V, I being most virulent and V the most attenuated) based on the mean survival time of rabbits after infection [10]. Due to the large size of the viral genome (161.8 kb) restriction fragment length polymorphism (RFLP) analysis of purified viral genomes has been the traditional method for the molecular differentiation of MV strains [6,11]. However, no correlation between RFLPs and attenuation has been demonstrated [6].Recent studies of field strains of MV have focussed on sequencing genes or gene fragments, however, the majority of these strains have not been characterised for virulence in rabbits [1,9]. Epidemiologi
A vueltas con la jubilación
Sarabia Cobo,Carmen María; Delgado Uría,Aroa; Castanedo Pfeiffer,Cristina;
Gerokomos , 2011, DOI: 10.4321/S1134-928X2011000200004
Abstract: the retirement age is considered the gate to determine the onset of old age. in recent years we are witnessing a political and social change regarding the appropriate age for retirement. a review of recent studies on the impact of retirement on people can afford to professionals adapt appropriate strategies to address these changes. this article provides a review of recent studies and hypotheses relating retirement and cognitive functioning and impact on quality of life.
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