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Search Results: 1 - 10 of 354 matches for " Armen Trchounian "
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Electronmicroscopic Study of Nanolike Bacteria  [PDF]
Karlen Hovnanyan, Margarita Hovnanyan, Armen Trchounian
Open Access Library Journal (OALib Journal) , 2017, DOI: 10.4236/oalib.1103485
Nanobacteria individual cases under the name of suggesting the bacteria mycoplasma and L-transformed forms of bacteria that imitate their size are nanobacteria that a separate systematic units remains controversial; the introduction of new high-resolution analytical microscopy techniques of prokaryote and eukaryote has revealed new structures in the structures of bacteria, which are the target of physical, chemical and biologically active stressors. The purpose of this work is, through a comparative retrospective analysis, to find out the motivation of “nanobacteria” ultrastructure public and visualize the structure of Gram-negative bacteria and viruses like symbiont of entamoeba.
Transmission and Scanning Electron Microscopy of Contacts between Bacterial and Yeast Cells in Biofilms on Different Surfaces  [PDF]
Karlen Hovnanyan, Seda Marutyan, Astghik Pepoyan, Liparit Navasardyan, Armen Trchounian
Open Access Library Journal (OALib Journal) , 2015, DOI: 10.4236/oalib.1101492
Abstract: The mechanism formation of colonies and biofilms of bacteria and yeasts are studied always of great interest. The aim of the presented work was transmission and scanning electron microscopic analysis contacts between cells of bacteria and yeast in biomofilms on natural structures and inorganic surface, as a result of formation of close contacts between a cellular wall, a fringe, cross-pieces, symplasts and cells of Escherichia coli, Shigella flexnerii Salmonella of typhi, Salmonella typhimurium and also some probiotic lactic acid on nutritious agar surfaces. Intercellular contacts in yeast biomofilms on plates of zirconium were scanning electron micro-scopic visualized by Candida guilliermondii.
The respiratory molybdo-selenoprotein formate dehydrogenases of Escherichia coli have hydrogen: benzyl viologen oxidoreductase activity
Basem Soboh, Constanze Pinske, Martin Kuhns, Mandy Waclawek, Christian Ihling, Karen Trchounian, Armen Trchounian, Andrea Sinz, Gary Sawers
BMC Microbiology , 2011, DOI: 10.1186/1471-2180-11-173
Abstract: Here we report the identification of a new H2: benzyl viologen oxidoreductase enzyme activity in E. coli that is independent of the [NiFe]-hydrogenases. This enzyme activity was originally identified after non-denaturing polyacrylamide gel electrophoresis and visualization of hydrogen-oxidizing activity by specific staining. Analysis of a crude extract derived from a variety of E. coli mutants unable to synthesize any [NiFe]-hydrogenase-associated enzyme activity revealed that the mutants retained this specific hydrogen-oxidizing activity. Enrichment of this enzyme activity from solubilised membrane fractions of the hydrogenase-negative mutant FTD147 by ion-exchange, hydrophobic interaction and size-exclusion chromatographies followed by mass spectrometric analysis identified the enzymes Fdh-N and Fdh-O. Analysis of defined mutants devoid of selenocysteine biosynthetic capacity or carrying deletions in the genes encoding the catalytic subunits of Fdh-N and Fdh-O demonstrated that both enzymes catalyze hydrogen activation. Fdh-N and Fdh-O can also transfer the electrons derived from oxidation of hydrogen to other redox dyes.The related respiratory molybdo-selenoproteins Fdh-N and Fdh-O of Escherichia coli have hydrogen-oxidizing activity. These findings demonstrate that the energy-conserving selenium- and molybdenum-dependent formate dehydrogenases Fdh-N and Fdh-O exhibit a degree of promiscuity with respect to the electron donor they use and identify a new class of dihydrogen-oxidizing enzyme.Hydrogen and formate are electron donors frequently used by anaerobic microorganisms. Metabolism of hydrogen and formate is often highly interlinked in many bacteria that can oxidize both compounds. This is exemplified in the fermentative metabolism of the enterobacterium Escherichia coli where up to one third of the carbon from glucose is converted to formate; formate is then disproportionated to H2 and CO2 [1-3]. Formate can be metabolized by three membrane-associated, molybd
New Design & Build Biological System through the Use of Microalgae Addressed to Sustainable Development  [PDF]
Armen B. Avagyan
Journal of Environmental Protection (JEP) , 2010, DOI: 10.4236/jep.2010.12023
Abstract: Current trends in energy consumption and biofuel manufacturing are neither secure nor sustainable, because they are not provided by necessary cost effective technologies. Further reductions of cost and technological development will be needed for biofuels to be able to compete effectively without subsidy. With the debate raging about raw material of biofuel, microalgae may offer a solution to this conundrum; creating enormous reserves of biofuels and boosting feed production. In this goal Center suggest projects, which incorporate water recourse management and restoration of lakes, freshwater conservation and cleanup through cost effective biodiesel manufacturing as well as pharmaceuticals destruction through the use of microalgae Chlorella and wastewaters aimed to replace the burning technology includes also supplying biofuel profitably and developed pilot bussiness plan based on the cost effective technology through applying new innovative approaches in various stages of microalgae production. The benefits of microalgae are so overwhelming that this, combined with the prospect of the improvement in nature protection, makes it imperative for the world to devise an international response and a plan of action. Incentives will be needed for the development of industry-led platforms such as the World Microalgae Technology Platform and its international financial fund. Microalgae must be the key tool for the new design and building sustainable development and environment management.
New Design of Biopharmaceuticals through the Use of Microalgae Addressed to Global Geopolitical and Economic Changes. Are You Ready for New Development in Biopharma?  [PDF]
Armen B. Avagyan
Pharmacology & Pharmacy (PP) , 2010, DOI: 10.4236/pp.2010.11005
Abstract: The Biopharma industry is enduring sweeping change in response to the financial crisis, but one aspect of the in-dustry that emerged relatively unscathed and that perhaps has directly benefited from the crisis is new revolutionary innova- tion solution. Identifying opportunities in the next wave of technologies for Biopharma, along with other policy initia- tives including financial crisis actions and climate policy, will affect on manufacturing biopharmaceutical products today and in the future in cost effective manner, and will be our adequate answer addressing to global geopolitical, economic and climate changes. It also underscores the search for new approach, evidenced by trends around new for- mulations to serve lower income patients. Microalgae biomass comes in many strains, and can be used by means of variety product developments. In the last years the key task of our R & D was to find a solution for these tasks. The bio- fuel market development dynamics include significant opportunity of microalgae raw material and microalgae proc- essing biomass rest of biodiesel manufacturing for Biopharma global growth in cost effective manner. Second new source of microalgae raw material for Biopharma include microalgae production through waste and wastewater cleaning. This should provide the opportunity to see the future in a new vision, where technology can serve as a revela- tion of the truth and where every endeavor is governed by reflection on and appreciation of the environment and thus leading to resolution of global tasks facing the world community and inclusion of microalgae in production and bio cycles open new cost effective ways for Biopharma companies and conservation of nature. A truly coherent microalgae raw material and Biopharma production policy has to find ways to bring these two traces closer for cost effective manufacturing, well being Biopharma economy and human health.
Correlations between delayed fluorescence of chlorophyll, metabolism and yield of plants. II. Influence of moisture of leaf and temperature condition on delayed fluorescence of leaves  [PDF]
Armen B. Avagyan
Journal of Biophysical Chemistry (JBPC) , 2010, DOI: 10.4236/jbpc.2010.11006
Abstract: During various temperatures of incubation the dehydration of leaves up to 3.2-3.8% mainly in-duced increase maximum amplitude of delayed fluorescence of chlorophyll. It was shown that moisture loss with this range could be deter-mined for the most part by the growth of the electrochemical potential of thylakoid mem-branes. The further incubation of detached leaves at 36℃ temperature, with more notable moisture loss, resulted in specific its decline as opposed to cases of 22 and 6℃ of thermal in-cubation. It was confirmed that the increased temperatures and moisture loss damage of the cells of plants occurred together induce a greater influence on plants than in case of oc-curring apart. The results allow to suppose that this can be mostly caused weakly associated polypeptides fallen out from the chloroplast membrane, which may be stipulated by high temperature combined with change ionic and osmotic stresses due moisture loss. Simulta-neously, the results showed that the exposure of the critical lowered air temperature led to considerable typical changes of leaves delayed fluorescence parameters of field plants. There-fore, their use can constitute new approaches to elucidate the molecular basis of plant freezing tolerance in a timely manner, based on concen-tration-related changes and the efficiency of coupling between light and dark processes of plants.
Correlations between delayed fluorescence of chlorophyll, metabolism and yield of plants. I. Influence of fertilizers on correlations  [PDF]
Armen B. Avagyan
Journal of Biophysical Chemistry (JBPC) , 2010, DOI: 10.4236/jbpc.2010.11005
Abstract: The increase of the potash fertilizer dose in-duced a raise in efficiency influence of the ni-trogen fertilizer, optimisation of phosphorous fertilizer effect, enhancement of leaf protein production, expansion of assimilating surface and yield growth. In the period of yield forma-tion, the parameters of delayed fluorescence of chlorophyll (DF) of leaf wholly corresponded with key factors that had a dramatic influence on the effectiveness of yield formation. The maximum level of DF amplitude mostly de-pended on the activity of nitrogen metabolism and presumably on active PSII concentration changes per square unit. Half-decay time of this amplitude was predominantly identified by the level of carbohydrate metabolism in the overall plant system, including the quantity of its products and, therefore, mostly by correspon-dence with yield. This is a biological base trig-gering the use of DF parameters for system analyses of plant production process.
Correlations between delayed fluorescence of chlorophyll, metabolism and yield of plants. III. Influence of viral infection on field plants and new technology of clone selection of virus-free planting potato  [PDF]
Armen B. Avagyan
Journal of Biophysical Chemistry (JBPC) , 2010, DOI: 10.4236/jbpc.2010.11007
Abstract: At the end of potato plants vegetation virus in-fection induced both decrease in maximum amplitude of delayed fluorescence maximal amplitude and increase half time of its decrease, as well as reduction in the amount of stems, plants’ height and assimilation area surface, yield, acceleration of plants development and their early die-off. The differences of DF pa-rameters and yields between strongly and weakly infected plants increase in case of a combined virus infection. In industrial test of the selection of virus-free planting potato by the use of DF parameter, a rise in the yield and de-crease degree of viral infection of crops was obtained.
Theory of Global Sustainable Development Based on Microalgae in Bio and Industrial Cycles, Management-Changing Decisions in Areas of Climate Change and Waste Management  [PDF]
Armen B. Avagyan
Journal of Sustainable Bioenergy Systems (JSBS) , 2013, DOI: 10.4236/jsbs.2013.34038

The paper provides requested management-changing decisions through implementation of conclusions of Global Sustainable Development theory based on including of microalgae in Bio and Industrial Cycles in the area of waste-related management challenges within creating market opportunities for industry through expansion of resource efficiency use across global supply chains and new design and building sustainable development with contemporary manufacturing of value added products. A truly coherent waste management and other production policy (biofuel, biopharmaceuticals, food, feed and perfumery additives) and mitigation of Climate Change are ways to bring these traces closer to cost effective manufacturing, improving of resource efficiency use, well being economy and human health. Offered technological change dramatically increase biomass feedstock resources, reduce waste origin of greenhouse emission (since 13%-17%), organics sent to landfill, pyrolyses, etc. and create a model that all elements along the waste value chain create economic, societal and/or environmental value.

3D Oscillator and Coulomb Systems reduced from Kahler spaces
Armen Nersessian,Armen Yeranyan
Physics , 2003, DOI: 10.1088/0305-4470/37/7/020
Abstract: We define the oscillator and Coulomb systems on four-dimensional spaces with U(2)-invariant Kahler metric and perform their Hamiltonian reduction to the three-dimensional oscillator and Coulomb systems specified by the presence of Dirac monopoles. We find the Kahler spaces with conic singularity, where the oscillator and Coulomb systems on three-dimensional sphere and two-sheet hyperboloid are originated. Then we construct the superintegrable oscillator system on three-dimensional sphere and hyperboloid, coupled to monopole, and find their four-dimensional origins. In the latter case the metric of configuration space is non-Kahler one. Finally, we extend these results to the family of Kahler spaces with conic singularities.
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