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Search Results: 1 - 10 of 32233 matches for " Antonio Felicioli "
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Exploring Proteins in Anopheles gambiae Male and Female Antennae through MALDI Mass Spectrometry Profiling
Francesca R. Dani, Simona Francese, Guido Mastrobuoni, Antonio Felicioli, Beniamino Caputo, Frederic Simard, Giuseppe Pieraccini, Gloriano Moneti, Mario Coluzzi, Alessandra della Torre, Stefano Turillazzi
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0002822
Abstract: MALDI profiling and imaging mass spectrometry (IMS) are novel techniques for direct analysis of peptides and small proteins in biological tissues. In this work we applied them to the study of Anopheles gambiae antennae, with the aim of analysing expression of soluble proteins involved in olfaction perireceptor events. MALDI spectra obtained by direct profiling on single antennae and by the analysis of extracts, showed similar profiles, although spectra obtained through profiling had a richer ion population and higher signal to noise ratio. Male and female antennae showed distinct protein profiles. MALDI imaging experiments were also performed and differences were observed in the localization of some proteins. Two proteins were identified through high resolution measurement and top-down MS/MS experiments. A 8 kDa protein only present in the male antennae matched with an unannotated sequence of the An. gambiae genome, while the presence of odorant binding protein 9 (OBP-9) was confirmed through experiments of 2-DE, followed by MS and MS/MS analysis of digested spots. This work shows that MALDI MS profiling is a technique suitable for the analysis of proteins of small and medium MW in insect appendices, and allows obtaining data for several specimens which can be investigated for differences between groups. Proteins of interest can be identified through other complementary MS approaches.
A Proteomic Investigation of Soluble Olfactory Proteins in Anopheles gambiae
Guido Mastrobuoni, Huili Qiao, Immacolata Iovinella, Simona Sagona, Alberto Niccolini, Francesca Boscaro, Beniamino Caputo, Marta R. Orejuela, Alessandra della Torre, Stefan Kempa, Antonio Felicioli, Paolo Pelosi, Gloriano Moneti, Francesca Romana Dani
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0075162
Abstract: Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are small soluble polypeptides that bind semiochemicals in the lymph of insect chemosensilla. In the genome of Anopheles gambiae, 66 genes encode OBPs and 8 encode CSPs. Here we monitored their expression through classical proteomics (2D gel-MS analysis) and a shotgun approach. The latter method proved much more sensitive and therefore more suitable for tiny biological samples as mosquitoes antennae and eggs. Females express a larger number and higher quantities of OBPs in their antennae than males (24 vs 19). OBP9 is the most abundant in the antennae of both sexes, as well as in larvae, pupae and eggs. Of the 8 CSPs, 4 were detected in antennae, while SAP3 was the only one expressed in larvae. Our proteomic results are in fairly good agreement with data of RNA expression reported in the literature, except for OBP4 and OBP5, that we could not identify in our analysis, nor could we detect in Western Blot experiments. The relatively limited number of soluble olfactory proteins expressed at relatively high levels in mosquitoes makes further studies on the coding of chemical messages at the OBP level more accessible, providing for few specific targets. Identification of such proteins in Anopheles gambiae might facilitate future studies on host finding behavior in this important disease vector.
Who Meets the Standrads: A Multidimensional Approach  [PDF]
Antonio Villar
Modern Economy (ME) , 2011, DOI: 10.4236/me.2011.24069
Abstract: We consider here the evaluation of the performance of a society with respect to a given set of targets. We provide a characterization of an intuitive evaluation formula that consists of the mean of the shares of the achievements in the targets. The criterion so obtained permits one not only to endogenously determine who meets the standards and who does not, but also to quantify the degree of fulfilment. Two empirical illustrations are provided: the compliance of the European Union Stability and Growth Pact, on the one hand, and the evaluation of research excellence in the Spanish universities, on the other hand.
The Educational Development Index: A Multidimensional Approach to Educational Achievements through PISA  [PDF]
Antonio Villar
Modern Economy (ME) , 2013, DOI: 10.4236/me.2013.45042

This paper proposes a multidimensional index that summarizes three relevant aspects of the educational achievements, out of the data provided by the PISA Report, concerning reading abilities of 15-year-old students from 65 countries. The three aspects considered are: performance, equity, and quality. The Educational Development Index (EDI) is the geometric mean of the normalized values of those three variables. We analyse the distribution of the variables that approach those three aspects and the resulting index, relative to the corresponding means of the OECD countries.

The Factorizational Theory of Finite Asymptotic Expansions in the Real Domain: A Survey of the Main Results  [PDF]
Antonio Granata
Advances in Pure Mathematics (APM) , 2015, DOI: 10.4236/apm.2015.51001

After studying finite asymptotic expansions in real powers, we have developed a general theory for expansions of type (*)\"\" ,x x0 where the ordered n-tuple \"\" forms an asymptotic scale at x0 , i.e.\"\" as x x0, 1 i n 1,

The Role of Asymptotic Mean in the Geometric Theory of Asymptotic Expansions in the Real Domain  [PDF]
Antonio Granata
Advances in Pure Mathematics (APM) , 2015, DOI: 10.4236/apm.2015.52013

We call “asymptotic mean” (at +∞) of a real-valued function \"\" the number, supposed to exist, \"\", and highlight its role in the geometric theory of asymptotic expansions in the real domain of type (*) \"\" where the comparison functions \"\", forming an asymptotic scale at +∞, belong to one of the three classes having a definite “type of variation” at +∞, slow, regular or rapid. For regularly varying comparison functions we can characterize the existence of an asymptotic expansion (*) by the nice property that a certain quantity F(t) has an asymptotic mean at +∞. This quantity is defined via a linear differential operator in f and admits of a remarkable geometric interpretation as it measures the ordinate of the point wherein that special curve \"\", which has a contact of order n -

Chronic Achilles Tendinopathy in Runners: Relationship between Pain and Tendon Vascularity and Efficacy and Safety of the Radial Extracorporeal Shock Wave Therapy  [PDF]
Antonio Ammendolia
Case Reports in Clinical Medicine (CRCM) , 2015, DOI: 10.4236/crcm.2015.46044
Few studies report the possible correlation pain-paratendon microvascularity during the painful phase of the chronic Achilles tendinopathy and the efficacy of the radial shock wave therapy re-spect to other therapies. The aim of the present longitudinal, controlled study is to demonstrate the variation of the tendon micro vascularization in athletes affected by Achilles tendinopathy and the efficacy and safety of the radial extracorporeal shock wave therapy. Twelve elite runners with Achilles tendinopathy were compared with 12 healthy amateurs, both treated by radial extracorporeal shock wave therapy in 3 sessions (1/every 3 days). VAS scale was used for pain evaluation at one and six months after treatment and a Color and Power Doppler echography was performed to observe the paratendon microvascularity before the beginning of the treatment and at one and six months after. One month after the beginning of the treatment, it was observed a decrease of the hypervascularity in all 12 subjects with tendinopathy and no variation in the control group participants. Clinically, 80% of patients referred pain relief and they were able to return to sports activity. The decrease of the paratendon microvascularity confirms the correlation between the disappearance of the pain and the normalization of the vascularity in the athletes. Moreover, radial extracorporeal shock wave therapy consented a quickly pain relief and returned to the sport. These results confirmed the efficacy and safety of this physical therapy that it could be considered a good therapeutic choice in the treatment of the chronic Achilles tendinopathy.
Analytic Theory of Finite Asymptotic Expansions in the Real Domain. Part II-C: Constructive Algorithms for Canonical Factorizations and a Special Class of Asymptotic Scales  [PDF]
Antonio Granata
Advances in Pure Mathematics (APM) , 2015, DOI: 10.4236/apm.2015.58047
Abstract: This part II-C of our work completes the factorizational theory of asymptotic expansions in the real domain. Here we present two algorithms for constructing canonical factorizations of a disconjugate operator starting from a basis of its kernel which forms a Chebyshev asymptotic scale at an endpoint. These algorithms arise quite naturally in our asymptotic context and prove very simple in special cases and/or for scales with a small numbers of terms. All the results in the three Parts of this work are well illustrated by a class of asymptotic scales featuring interesting properties. Examples and counterexamples complete the exposition.
Analytic Theory of Finite Asymptotic Expansions in the Real Domain. Part II-B: Solutions of Differential Inequalities and Asymptotic Admissibility of Standard Derivatives  [PDF]
Antonio Granata
Advances in Pure Mathematics (APM) , 2015, DOI: 10.4236/apm.2015.58046
Abstract: Part II-B of our work continues the factorizational theory of asymptotic expansions of type (*) \"\"\"\",\"\" , \"\" where the asymptotic scale \"\", \"\" , is assumed to be an extended complete Chebyshev system on a one-sided neighborhood of x0. The main result states that to each scale of this type it remains as-sociated an important class of functions (namely that of generalized convex functions) enjoying the property that the expansion (*), if valid, is automatically formally differentiable n ? 1 times in the two special senses characterized in Part II-A. A second result shows that formal applications of ordinary derivatives to an asymptotic expansion are rarely admissible and that they may also yield skew results even for scales of powers.
Analytic Theory of Finite Asymptotic Expansions in the Real Domain. Part II-A: The Factorizational Theory for Chebyshev Asymptotic Scales  [PDF]
Antonio Granata
Advances in Pure Mathematics (APM) , 2015, DOI: 10.4236/apm.2015.58045
Abstract: This paper, divided into three parts (Part II-A, Part II-B and Part II-C), contains the detailed factorizational theory of asymptotic expansions of type (?)?\"\", \"\", \"\", where the asymptotic scale?\"\", \"\", is assumed to be an extended complete Chebyshev system on a one-sided neighborhood of . It follows two pre-viously published papers: the first, labelled as Part I, contains the complete (elementary but non-trivial) theory for \"\"; the second is a survey highlighting only the main results without proofs. All the material appearing in §2 of the survey is here reproduced in an expanded form, as it contains all the preliminary formulas necessary to understand and prove the results. The remaining part of the survey—especially the heuristical considerations and consequent conjectures in §3—may serve as a good introduction to the complete theory.
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