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Search Results: 1 - 10 of 1220 matches for " Antonella Gagliardi "
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Escherichia coli Population-Based Study in Pediatric Crohn’s Disease  [PDF]
Serena Schippa, Valentina Totino, Massimiliano Marazzato, Maria Stefania Lepanto, Floriana Santangelo, Marta Aleandri, Antonella Gagliardi, Catia Longhi, Fabrizio Pantanella, Valerio Iebba, Maria Pia Conte
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.413098

Escherichia coli is assumed to be involved in inflammatory bowel disease (IBD) by many authors. The present Short Report was aimed at analyzing E. coli population isolated from ileal samples collected from 4 CD and 3 non-CD (control group) diagnosed pediatric patients. A total of 539 mucosa-associated E. coli strains were characterized by: Random Amplified Polymorphic DNA (RAPD), adhesive and virulence factors, and for their phylogenetic groups. A significant separation between RAPD profiles of the two CD and non-CD cohorts (P < 0.0001), along with a significant reduction of intra-species genomic variability in E. coli populations isolated from CD group (P < 0.0001) was found as assessed by Dice index, indicating a different selective pressure in CD intestinal habitat. A predominance of phylogenetic group A was found in control subjects (P < 0.0001). Results on adhesive and virulence factors evidenced peculiar genes significantly related to CD E. coli populations (K1, IbeA) (P < 0.0001) and to total DNA from biopsy specimens K1 (P < 0.0001). Results suggest that among E. coli population, particular variants may be favorite in the intestinal habitat of CD pediatric patients. These genotype variants could represent the genetic background that, via evolutionary phenomena driven by a persistent inflammatory state, may evolve in Adherent Invasive Escherichia coli (AIEC) like strains found in adult CD patients.

Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Allelic Variants Relate to Shifts in Faecal Microbiota of Cystic Fibrosis Patients
Serena Schippa, Valerio Iebba, Floriana Santangelo, Antonella Gagliardi, Riccardo Valerio De Biase, Antonella Stamato, Serenella Bertasi, Marco Lucarelli, Maria Pia Conte, Serena Quattrucci
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0061176
Abstract: Introduction In this study we investigated the effects of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) gene variants on the composition of faecal microbiota, in patients affected by Cystic Fibrosis (CF). CFTR mutations (F508del is the most common) lead to a decreased secretion of chloride/water, and to mucus sticky secretions, in pancreas, respiratory and gastrointestinal tracts. Intestinal manifestations are underestimated in CF, leading to ileum meconium at birth, or small bowel bacterial overgrowth in adult age. Methods Thirty-six CF patients, fasting and under no-antibiotic treatment, were CFTR genotyped on both alleles. Faecal samples were subjected to molecular microbial profiling through Temporal Temperature Gradient Electrophoresis and species-specific PCR. Ecological parameters and multivariate algorithms were employed to find out if CFTR variants could be related to the microbiota structure. Results Patients were classified by two different criteria: 1) presence/absence of F508del mutation; 2) disease severity in heterozygous and homozygous F508del patients. We found that homozygous-F508del and severe CF patients exhibited an enhanced dysbiotic faecal microbiota composition, even within the CF cohort itself, with higher biodiversity and evenness. We also found, by species-specific PCR, that potentially harmful species (Escherichia coli and Eubacterium biforme) were abundant in homozygous-F508del and severe CF patients, while beneficial species (Faecalibacterium prausnitzii, Bifidobacterium spp., and Eubacterium limosum) were reduced. Conclusions This is the first report that establishes a link among CFTR variants and shifts in faecal microbiota, opening the way to studies that perceive CF as a ‘systemic disease’, linking the lung and the gut in a joined axis.
Systems Approach to Mitotic Chromosome Motions  [PDF]
Lucian John Gagliardi
Open Journal of Biophysics (OJBIPHY) , 2013, DOI: 10.4236/ojbiphy.2013.32017
Abstract: Recent experiments revealing possible nanoscale electrostatic interactions in force generation at kinetochores for chromosome motions have prompted speculation regarding possible models for interactions between positively charged molecules in kinetochores and negative charge on C-termini near the plus ends of microtubules. A clear picture of how kinetochores establish and maintain a dynamic coupling to microtubules for force generation during the complex motions of mitosis remains elusive. The molecular cell biology paradigm requires that specific molecules, or molecular geometries, for force generation be identified. However, it is possible to account for mitotic chromosome motions within a systems approach in terms of experimentally known cellular electric charge distributions interacting over nanometer distances.

Postoperative Delirium after elective and emergency surgery: analysis and checking of risk factors. A study protocol
Vanni Agnoletti, Luca Ansaloni, Fausto Catena, Rabbih Chattat, Angelo De Cataldis, Gianfranco Di Nino, Claudio Franceschi, Stefano Gagliardi, Rita Melotti, Antonella Potalivo, Mario Taffurelli
BMC Surgery , 2005, DOI: 10.1186/1471-2482-5-12
Abstract: All patients admitted to our Emergency Unit who are meet our inclusion/exclusion criteria will be recruited. The arising of postoperative Delirium will select incidentally two groups (Delirium/non Delirium) and the forward analysis of correlate risk factors will be performed. As in a typical observational case/control study we will consider all the exposure factors to which our population are submitted towards the outcome (presence of Delirium). Our exposures are the following: ASA, Pain (SVS; VAS), Blood gas analysis (pH; Hb; pO2; pCO2), Residence pharmacological therapy (BDZ; hypnotics; narcotic drugs; alcohol; nitrous derivates), Body temperature, Arterial pressure, Heart frequency, Breath frequency, Na, K, Creatinin, Glicemia, Albumin, Hct, White blood cells, Glasgow Coma Scale (GCS), Cognitive state (SPMSQ), Functional state (ADL and IADL), Psychological Distress (HADS), Cumulative Illness Rating Scale (CIRS), Hypotension (classified in: light; moderate and severe and duration), Blood loss (classified in: < 2 lt and > 2 lt), Blood transfusions (< 2 lt and > 2 lt), Quantity of red cells and plasma transfusions, Visual VAS / SVS (timing: I-II-III post-operative day), Red cells and Plasma transfusions, Blood count evaluation and Saturation (O2%), Postoperative analgesia (Emilia-Romagna protocol), Presence of malignant tumoral disease, APACHE Score II. Moreover the presence of some relevant genetic polymorphisms will be studied in different genes such as IL-6, IL-10, TNF-alpha, and IL-1 cluster.Delirum is common in hospitalized elderly patients and may be associated with increased morbidity[1], length of stay and patient care costs[2]. The classic manifestations of this syndrome are impaired cognition and decreased ability to maintain attention[3]. Efforts to understand this syndrome require a thorough understanding of its causes and the ability to predict who is at risk. Each year Delirium complicates hospital stays for more than 2.3 milion older people, involves
Inhibition of cell growth by EGR-1 in human primary cultures from malignant glioma
Antonella Calogero, Vincenza Lombari, Giorgia De Gregorio, Antonio Porcellini, Severine Ucci, Antonietta Arcella, Riccardo Caruso, Franco Gagliardi, Alberto Gulino, Gaetano Lanzetta, Luigi Frati, Dan Mercola, Giuseppe Ragona
Cancer Cell International , 2004, DOI: 10.1186/1475-2867-4-1
Abstract: Low levels of EGR-1 protein were found in all primary cultures examined, with lower values present in grade IV tumors and in cultures carrying wild-type copies of p53 gene. The levels of EGR-1 protein were significantly correlated to the amount of intracellular fibronectin, but only in tumors carrying wild-type copies of the p53 gene (R = 0,78, p = 0.0082). Duplication time, plating efficiency, colony formation in agarose, and contact inhibition were also altered in the p53 mutated tumor cultures compared to those carrying wild-type p53. Growth arrest was achieved in both types of tumor within 1–2 weeks following infection with a recombinant adenovirus overexpressing EGR-1 but not with the control adenovirus.Suppression of EGR-1 is a common event in gliomas and in most cases this is achieved through down-regulation of gene expression. Expression of EGR-1 by recombinant adenovirus infection almost completely abolishes the growth of tumor cells in vitro, regardless of the mutational status of the p53 gene.EGR-1 encodes a nuclear phosphoprotein that binds to DNA and regulates transcription through a GC-rich consensus sequence [1-4]. EGR-1 is involved in the regulation of cell responses to a wide array of stimuli such as mitogens, growth factors and stress stimuli [5-7]. Recent studies have shown that EGR-1 expression is altered in several types of neoplasia, compared to normal tissue [1,8,9]. Gene deletion or EGR-1 mutations have been reported in sporadic hematological malignancies [10]. EGR-1 expression has been found to be either decreased or undetectable in human breast cancer tissue and small cell lung carcinoma [11,12]. EGR-1 is altered in a different manner in prostate cancer, where higher levels of EGR-1 expression are found correlated to more advanced stages of malignancy [13]. Later studies confirmed in two independent mouse models that EGR-1 up-regulates tumor progression [14,15]. From these various studies it is clear that EGR-1 is involved in regulation of
Higher Prevalence and Abundance of Bdellovibrio bacteriovorus in the Human Gut of Healthy Subjects
Valerio Iebba, Floriana Santangelo, Valentina Totino, Mauro Nicoletti, Antonella Gagliardi, Riccardo Valerio De Biase, Salvatore Cucchiara, Lucia Nencioni, Maria Pia Conte, Serena Schippa
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0061608
Abstract: Introduction Members of the human intestinal microbiota are key players in maintaining human health. Alterations in the composition of gut microbial community (dysbiosis) have been linked with important human diseases. Understanding the underlying processes that control community structure, including the bacterial interactions within the microbiota itself, is essential. Bdellovibrio bacteriovorus is a gram-negative bacterium that preys other gram-negative species for survival, acting as a population-balancer. It was found in terrestrial/aquatic ecosystems, and in animal intestines, postulating its presence also in the human gut. Methods The present study was aimed to evaluate, by end-point PCR and qPCR, the presence of B. bacteriovorus in intestinal and faecal biopsy specimens from 92 paediatric healthy subjects and patients, suffering from Inflammatory Bowel Diseases (IBD), Celiac disease and Cystic fibrosis (CF). Results i) B. bacteriovorus was present and abundant only in healthy individuals, while it was heavily reduced in patients, as in the case of IBD and Celiac, while in CF patients and relative controls we observed comparable results; ii) B. bacteriovorus seemed to be mucosa-associated, because all IBD and Celiac biopsies (and related controls) were treated with mucus-removing agents, leaving only the mucosa-attached microflora; iii) B. bacteriovorus abundance was district-dependent, with a major preponderance in duodenum, and gradually decreasing up to rectum; iv) B. bacteriovorus levels significantly dropped in disease status, in duodenum and ileum. Conclusions Results obtained in this study could represent the first step for new therapeutic strategies aimed to restore a balance in the intestinal ecosystem, utilizing Bdellovibrio as a probiotic.
O problema da autoria na teoria literária: apagamentos, retomadas e revis?es
Gagliardi, Caio;
Estudos Avan?ados , 2010, DOI: 10.1590/S0103-40142010000200018
Abstract: the latest discussions about authorship are closely related to the searching for the meaning of the text. because of that, the authorship is being understood as one of the most controversial subjects in literature theory. the first two parts of this paper comprehend a critic and sistematic analysis of 1. the author refusal as source of the text meaning (proust, eliot, croce, wimsatt and beardsley, derrida, barthes and foucault), and 2. the defense and reviewing of this concept (booth, bloom, eco e compagnon). and in the last part, 3. it is proposed a particular hypothesis of analises and interpretation of the authorship, through a change of contexts: the recurrence to the heteronomy concept, by fernando pessoa.
La rivoluzione dell’igiene zootecnica
Giorgio Gagliardi
Veterinaria Italiana , 2010,
Abstract: Le cose troppo lontane o troppo vicine, troppo grandi o piccole, troppo veloci o lente sfuggono ai sensi e, quindi, all’attenzione immediata dei contemporanei, mentre i posteri possono mettere a fuoco le vicende umane nella loro globalità, meglio di chi le ha viste direttamente nel loro evolversi.Dopo il marasma della Grande Guerra, la Zootecnia italiana ripartì con grande volontà di recupero e altrettanto disordine sanitario portando ad una situazione epidemiologica molto complessa che si risolse, progressivamente, durante gli ultimi decenni del secolo scorso. All’inizio dell'ultima decade del secolo scorso il Paese si è trovato in una buona situazione zoosanitaria che oggi permette, soprattutto a chi ha vissuto il fenomeno nel suo complesso, di rivedere il passato con maggiore cognizione, esaminare cause ed effetti, trarre conclusioni epidemiologiche di fondamentale interesse.
Organizations as Designed Islands
Pasquale Gagliardi
Island Studies Journal , 2009,
Abstract: The literature and practice of organizational design are mostly based on simplistic conceptions which ignore recent theoretical developments in organizational studies. Conceiving of organizations as ‘designed islands’, it is argued, can contribute to a more solid theoretical foundation to organization theory, viewed as normative science. Relying on the work of Peter Sloterdijk, who describes the forms of life in space in terms of spheres, the heuristic power of the island metaphor is explored. What can be learnt from the art of isolating in order to construct lived organizational environments is then discussed, and the paradoxical relationship between connection and isolation is highlighted.
The Cox ring of a spherical embedding
Giuliano Gagliardi
Mathematics , 2012, DOI: 10.1016/j.jalgebra.2013.08.037
Abstract: Let G be a connected reductive group and G/H a spherical homogeneous space. We show that the ideal of relations between a natural set of generators of the Cox ring of a G-embedding of G/H can be obtained by homogenizing certain equations which depend only on the homogeneous space. Using this result, we describe some examples of spherical homogeneous spaces such that the Cox ring of any of their G-embeddings is defined by one equation.
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