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Search Results: 1 - 10 of 156213 matches for " Ant?nio F.;Moita "
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The Cadherin Superfamily in Anopheles gambiae: a Comparative Study With Drosophila melanogaster
Catarina Moita,Sérgio Sim es,Luís F. Moita,António Jacinto,Pedro Fernandes
Comparative and Functional Genomics , 2005, DOI: 10.1002/cfg.473
Abstract: The cadherin superfamily is a diverse and multifunctional group of proteins with extensive representation across genomes of phylogenetically distant species that is involved in cell–cell communication and adhesion. The mosquito Anopheles gambiae is an emerging model organism for the study of innate immunity and host–pathogen interactions, where the malaria parasite induces a profound rearrangement of the actin cytoskeleton at critical stages of infection. We have used bioinformatics tools to retrieve present sequence knowledge about the complete repertoire of cadherins in A. gambiae and compared it to that of the fruit fly, Drosophila melanogaster. In A. gambiae, we have identified 43 genes coding for cadherin extracellular domains that were re-annotated to 38 genes and represent an expansion of this gene family in comparison to other invertebrate organisms. The majority of Drosophila cadherins show a 1 : 1 Anopheles orthologue, but we have observed a remarkable expansion in some groups in A. gambiae, such as N-cadherins, that were recently shown to have a role in the olfactory system of the fruit fly. In vivo dsRNA silencing of overrepresented genes in A. gambiae and other genes showing expression at critical tissues for parasite infection will likely advance our understanding of the problems of host preference and host𕢓pathogen interactions in this mosquito species.
Produ??o comercializável e teores de Cu e Zn em cenoura em decorrência da a??o residual de fósforo e composto de lixo em solo sob cerrado
Mesquita Filho, Manoel V. de;Souza, Antnio F.;Moita, Antnio W.;Ramagem, Ricardo D.;
Horticultura Brasileira , 2002, DOI: 10.1590/S0102-05362002000200006
Abstract: a field experiment was conducted on a clayey yellow red oxisol to evaluate the residual effect of the application of phosphorus and urban waste compost of the previous two years on the root production of carrot cv. brasília. the soil of the previous experiment design used a factorial consisting of three levels of phosphorus (0; 400 and 800 kg ha-1), applied as triple superphosphate combined with five levels of urban waste compost (0; 20; 40; 60 and 80 t.ha-1), was arranged in randomized complete blocks with three replicates. carrot plants were harvested 90 days after planting. after the harvest, a linear and quadratic effect for phosphorus and urban waste compost (p<0,01) was observed. the linear interaction p x quadratic urban compost was highly significant (p<0,01). the maximum root total production was 26.5 t.ha-1 corresponding to 18.5 t ha-1 of marketable yield, estimated by the calculated doses of 762.5 kg ha-1 of p2o5 and 53.2 t ha-1 of urban waste compost according to the function: y(prod) = 4.541143 + 4.0088 x 10-2 p2o5 + 2.50486 x 10-1 clx - 2.5619 x 10-5 (p2o5)2 - 1.9125 x 10-5 (clx * p2o5) - 2.216 x 10-3 clx2 (r2 = 0,96), where clx = urban waste compost. doses of urban waste compost applied to the soil, affected the values of ph, electric conductivity, and the available contents of cu and zn. even under the highest levels of phosphorus and urban waste compost applied in the experiment, no phytotoxic effect on carrot plants was observed, and in the edible part, none of the elements reached the maximum tolerant limit for food, as established by the brazilian legislation.
Produ o comercializável e teores de Cu e Zn em cenoura em decorrência da a o residual de fósforo e composto de lixo em solo sob cerrado
Mesquita Filho Manoel V. de,Souza Antnio F.,Moita Antnio W.,Ramagem Ricardo D.
Horticultura Brasileira , 2002,
Abstract: Realizou-se em 1997, em condi es de campo um experimento em Latossolo Vermelho Escuro distrófico argiloso sob cerrado de Brasília, para avaliar o efeito residual das aplica es em anos anteriores, a lan o de doses de fósforo (superfosfato triplo), e de composto de lixo na produ o de cenoura (Daucus carota), cv. Brasília, assim como nos teores de cobre e zinco em raízes frescas. Aproveitou-se o mesmo delineamento experimental de blocos ao acaso com 3 repeti es, no mesmo campo experimental dos experimentos anteriores distribuídos num esquema fatorial 3 x 5 incluindo-se 3 níveis de fósforo (0; 400 e 800 kg ha-1 de P2O5) e 5 níveis de composto de lixo (0; 20; 40; 60 e 80 t ha-1). A colheita foi realizada aos 90 dias após o plantio. A análise estatística dos dados de produ o total de raízes revelou efeito residual da aduba o dos últimos dois anos em linear e quadrático altamente significativo (p<0,01) para fósforo e composto de lixo. A intera o linear de P x quadrática composto de lixo foi altamente significativa (p<0,01). A produ o total máxima de 26,5 t ha-1, correspondendo a 18,5 t ha-1 de raízes comercializáveis, foi assegurada pelas doses calculadas de 762,5 kg ha-1 de P2O5 e 53,2 t ha-1 de composto de lixo de acordo com a fun o Y(PROD) = 4,541143 + 4,0088 x 10-2 P2O5 + 2,50486 x 10-1 CLx - 2,5619 x 10-5 (P2O5)2 - 1,9125 x 10-5 (CLx * P2O5) - 2,216 x 10-3 CLx2 (R2 = 0,96), onde CLx = composto de lixo. A aplica o ao solo das doses de composto de lixo alteraram o pH, a condutividade elétrica e os teores de Cu e Zn. Mesmo nas doses mais elevadas de fósforo e de composto de lixo aplicadas n o foram observados efeitos fitotóxicos de Cu ou de Zn. Os teores desses elementos em raízes in natura estiveram abaixo dos permitidos pela legisla o brasileira de alimentos.
Redu??o de perdas pós-colheita em tomate de mesa acondicionado em três tipos de caixas
Luengo, Rita Fátima A.;Moita, Antnio Williams;Nascimento, Edson F.;Melo, Mário F.;
Horticultura Brasileira , 2001, DOI: 10.1590/S0102-05362001000200012
Abstract: the most common box used for harvested vegetables in brazil is the wood one which was used for kerosene transporting during second world war, in 1945. this box causes mechanical damage and reduces vegetables shelf-life and quality, due to its rough surface allowing pathogen colonization, due to the excessive number of fruit layers, and due to the lateral cut openings. considering the problems of the k box and the necessity of protecting tomato fruits, embrapa hortali?as began in january 1997 a research to develop an adequate box to protect tomato fruits. the definitive box, named "embrapa box", was compared with the k box and the most common plastic boxes from the market. the weight, shelf-life, color, mechanical damage, firmness, relative water content and deterioration were evaluated. the damage was significantly different and lower in the embrapa box, reducing post-harvest losses in tomato fruits.
Identifica o das causas de perdas pós-colheita de cenoura no varejo, Brasília-DF
Lana Milza M.,Moita Antnio Williams,Nascimento Edson F. do,Souza Geraldo da S. e
Horticultura Brasileira , 2002,
Abstract: As perdas pós-colheita de cenoura em 4 lojas de uma rede de supermercados de Brasília foram avaliadas pelo período de um ano. A amostragem foi realizada em duas etapas: antes da exposi o do produto na área de vendas (amostra Qualidade Inicial) e após o descarte das perdas do produto (amostra Descarte). Foram quantificadas a propor o de raízes pequenas, médias e grandes e a incidência dos danos: bifurca o, rachadura, defeitos de formato, doen a, praga, dano mecanico e outros. A cenoura comprada pelo supermercado apresentou cerca de 86,88 + 0,66% das raízes na faixa de 12-22 cm. No descarte, diminuiu a propor o de cenouras médias e grandes, enquanto aumentou a propor o de cenouras pequenas e quebradas. A propor o de raízes com ombro verde foi reduzida de 23,74 + 1,11% na amostra Qualidade Inicial para 13,29 + 1,37% na amostra Descarte, indicando que grande parte das raízes com ombro verde foi adquirida pelo consumidor, e neste caso n o parece ter sido um fator indutor de perda. Cerca de 51% das cenouras recebidas no supermercado apresentavam algum tipo de dano, sendo os mais importantes defeitos de formato (31,0 + 1,16%), dano mecanico (9,46 + 0,4%) e murcha (4,66 + 0,99%). As principais causas de descarte foram dano mecanico (37,4 + 2,47%), defeitos de formato (32,0 + 2,58%) e murcha (7,6 + 1,59%).
Identifica??o das causas de perdas pós-colheita de cenoura no varejo, Brasília-DF
Lana, Milza M.;Moita, Antnio Williams;Nascimento, Edson F. do;Souza, Geraldo da S. e;Melo, Mário Felipe de;
Horticultura Brasileira , 2002, DOI: 10.1590/S0102-05362002000200026
Abstract: carrot post-harvest losses were evaluated for one year in four stores of a supermarket chain in brasilia, brazil. sampling was performed at two stages: at reception in the store and after exclusion or removal from retail displays. the proportion of large, middle, small and broken roots was measured. damaged roots were classified in one of the following categories and measured: splitting, forking, mishaped roots, pathological breakdown, pest damage, mechanical damage, and unidentified damage. about 86,88 + 0,66% of the roots were 12-22 cm long (middle size). wastage presented a higher proportion of small and broken roots and a lower proportion of middle and large roots. the incidence of green top in waste decreased, when compared to the sample taken before marketing, from 23,74 + 1,11% to 13,29 + 1,37%. this is an indication that a substantial proportion of roots with green tops were sold and therefore this defect do not seem to be a factor inducing loss. about 51% of the roots delivered at the supermarket were damaged and the most important defects were mishaped roots (31,0 + 1,16%), mechanical damage (9,46 + 0,4%), and wilting (4,66 + 0,99%). the main causes of waste were mechanical damage (37,4 + 2,47%), mishaped roots (32,0 + 2,58%) and wilting (7,6 + 1,59%).
Efeito da descompacta??o profunda de solo na produ??o da cultura da batata
Ragassi, Carlos Francisco;Favarin, José Laércio;Shiraishi, Fábio Akio;Moita, Antnio Williams;Sako, Henry;Melo, Paulo César T de;
Horticultura Brasileira , 2009, DOI: 10.1590/S0102-05362009000400014
Abstract: the soil tillage for potato in brazil commonly relies on harrow utilization (shallow tillage), which causes a compacted zone below 20 cm. apparently, deep soil loosening improves productivity and reduces tuber disease incidence and these effects can vary according to the species of grass cultivated before the potato crop. the objective of this work was to study deep loosening associated to the cultivation of panicum maximum cv. tanzania, brachiaria brizantha cv. marandu and maize (zea mays) 'dekalb 191' and the control (shallow tillage with the same maize genotype). the experiment was carried out in piracicaba, s?o paulo state, brazil, from december 2006 to october 2008, in a complete randomized blocks design. the soil penetration resistance (spr) value, evaluated by impact penetrometer in the center of the seed bed, was lower than 1.5 mpa up to 40 cm depth for all treatments and, in the 40-60 cm layer, the spr value in the shallow tillage (2.4 mpa) was statistically higher than all other treatments, whose values ranged from 0.9 to 1.0 mpa. tuber productivity in the shallow tillage was 28.3 t ha-1 and differed from the highest value among the deep soil loosening treatments (32.9 t ha-1). the proportion of tubers with less than 4 cm diameter in the shallow tillage (5.1%) was higher than the deep soil alleviation treatments with maize (2.9%) and b. brizantha (2.2%). the occurrence of tuber pests, diseases and lenticelose (diabrotica speciosa, 31.0% to 49.7%; streptomyces scabies, 3.3% to 6.3%; helminthosporium solani, 60.3% to 69%; rhizoctonia solani, 1.3% to 4.3%; lenticelose, 6.0% to 15.7%), was not influenced by the treatments. the deep soil loosening reduced soil penetration resistance and increased potato productivity, with lower rate of small tubers, but did not affect the occurrence of tuber pests, diseases and lenticelose.
Detec??o por sorologia do Melon yellowing associated virus (MYaV) em áreas produtoras de mel?o no Nordeste brasileiro
Lima, Mirtes F;Nagata, Tatsuya;Neves, Filipe M;Inoue-Nagata, Alice K;Moita, Antnio W;Sousa, Carla;Vecchia, Marília Della;Rangel, Maurício G;Dias, Rita de CS;Dutra, Luiza S;ávila, Antnio C de;
Horticultura Brasileira , 2009, DOI: 10.1590/S0102-05362009000400013
Abstract: the northeast region of brazil is the main melon-producing region of the country, being responsible for more than 90% of the total national production. a new disease, known as "yellowing of melon plants", which has been associated to a new viral agent, the melon yellowing-associated virus (myav), has been reported to cause damage on this crop since 1999. in this study we evaluated the occurrence of the myav in melon plants exhibiting suspicious symptoms of the disease in major melon growing states of the northeast region. in november 2007, commercial melon fields were inspected for the occurrence of this virus. a total of 374 plants was collected in melon fields of the states of rio grande do norte (54) and ceará (37) and in the submédio do vale do s?o francisco (283), in bahia and pernambuco states. sample evaluation was performed by das-elisa using polyclonal antibodies developed at the embrapa hortali?as for myav detection. extracts prepared from leaves and stems of symptomatic plants were used as antigen. the myav was detected in 58.0% of the collected samples. interestingly, the virus concentration was higher in stems than in leaves. the incidence of myav was higher in samples collected from rio grande do norte (96.3%) and ceará (75.7%) fields than from those in the submédio do vale do s?o francisco (pernambuco and bahia, with 48.4%). these data confirmed the widespread occurrence of the virus in melon fields of the main melon-producing areas of northeastern brazil and the efficiency of the antibody for myav detection.
Reaction of coffee genotypes to different populations of Meloidogyne spp.: detection of a naturally virulent M. exigua population
Muniz, Maria de Fátima S.;Campos, Vicente P.;Moita, Antnio W.;Gon?alves, Wallace;Almeida, Maria Ritta A.;Sousa, Fábio Rodrigues de;Carneiro, Regina Maria D. G.;
Tropical Plant Pathology , 2009, DOI: 10.1590/S1982-56762009000600002
Abstract: the reaction of seven genotypes of coffea arabica to 10 meloidogyne spp. populations collected mainly from coffee plantations in brazil and costa rica was evaluated under greenhouse conditions. the inoculum consisted of 10,000 eggs per plant. evaluations were done 8 months after inoculations considering the root fresh weight, gall and egg mass indices, number of eggs per gram of root and reproduction factor (rf). the cultivars obat? iac 1669-20, sarchimor iac 4361 and tupi amarelo iac 5111 exhibited susceptibility to the four brazilian m. exigua populations tested. however, cv. tupi vermelho iac 1669-33 revealed resistance (rf value of 0.7) to the m. exigua population from lavras, minas gerais state, brazil. a population of m. exigua from bom jesus de itabapoana, rio de janeiro state, brazil, was highly virulent on cv. iapar 59 (rf= 165.7), bearing resistance gene mex-1, and was also virulent on genotype paraíso (h 419-5-4-5-2) (rf=396.2). a meloidogyne sp. population on coffee from gar?a, s?o paulo state, brazil, reproduced at low rates (rf ranging from 0.1 to 3.9) on all genotypes. all tested cultivars were susceptible to m. incognita and m. paranaensis. m. mayaguensis of guava from paraná state, brazil, reproduced at low rates in all coffee genotypes; however, another population of coffee, from costa rica, was more aggressive and showed rf value that ranged from 0.8 to 12.4. results of this study point for the first time to the ability of a naturally occurring m. exigua population to overcome the resistance conferred by the mex-1 gene.
Highly Dynamic Host Actin Reorganization around Developing Plasmodium Inside Hepatocytes
Carina S. S. Gomes-Santos, Maurice A. Itoe, Cristina Afonso, Ricardo Henriques, Rui Gardner, Nuno Sepúlveda, Pedro D. Sim?es, Helena Raquel, António Paulo Almeida, Luis F. Moita, Friedrich Frischknecht, Maria M. Mota
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0029408
Abstract: Plasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside a parasitophorous vacuole. The nature of the Plasmodium-host cell interface, as well as the interactions occurring between these two organisms, remains largely unknown. Here we show that highly dynamic hepatocyte actin reorganization events occur around developing Plasmodium berghei parasites inside human hepatoma cells. Actin reorganization is most prominent between 10 to 16 hours post infection and depends on the actin severing and capping protein, gelsolin. Live cell imaging studies also suggest that the hepatocyte cytoskeleton may contribute to parasite elimination during Plasmodium development in the liver.
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