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Search Results: 1 - 10 of 14688 matches for " António Amorim "
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The Interaction of Monetary and Fiscal Policy: The Brazilian Case  [PDF]
Tito Belchior Silva Moreira, Fernando Antnio Ribeiro Soares, Adolfo Sachsida, Paulo Roberto Amorim Loureiro
Modern Economy (ME) , 2011, DOI: 10.4236/me.2011.22016
Abstract: We tested, empirically, whether the Brazilian fiscal policy for the period between 1995:I to 2008:III was active or passive. To analyze fiscal policy transmission mechanisms, we estimated functions by which the public debt/GDP ratio affects investment, primary surplus, output gap and the demand for money. The ratio of public debt to GDP was found to be statistically significant, positively affecting the demand for money and the primary surplus, whereas it was found to negatively affect the level of investment and the output gap. We conclude that the Brazilian regime was non-Ricardian in the context of fiscal dominance.
PAH mutational spectrum: still expanding  [PDF]
Laura Vilarinho, Sofia Esteves, Elisabete Ramos, António Amorim, Luisa Azevedo
Open Journal of Genetics (OJGen) , 2011, DOI: 10.4236/ojgen.2011.12002
Abstract: Phenylketonuria (PKU, MIM 261600) is the most common inborn error of amino acid metabolism. To date, a total of more than 500 mutations have been associated with the disease. In this report, the novel p.Glu182Lys mutation, found in a Portuguese family in combination with the previously reported p.Leu 348Val, is presented and its putative deleterious impact discussed.
Políticas de identidade: perfil de DNA e a identidade genético-criminal
Machado,Helena; Silva,Susana; Amorim,António;
Análise Social , 2010,
Abstract: dna is seen by many as the ?true? basis of human identity, insofar as it is a biological structure that is, in principle, unique in each individual. this notion of ?uniqueness?, a fundamental pillar of criminal investigation and forensic genetics, has fostered identity politics by modern states through the classification and storage of information about ?criminals?. this article explores the alignment of science and state bureaucracy for producing the ?genetic-criminal? identity in the context of the portuguese forensic dna database for forensic purposes. we discuss the impacts of this sort of identity politics for the management, categorization, and surveillance of individuals classified as criminals.
Consórcios de bibliotecas no Brasil: um desafio à democratiza??o do conhecimento
Amorim, Antnio Marcos;Vergueiro, Waldomiro;
Perspectivas em Ciência da Informa??o , 2006, DOI: 10.1590/S1413-99362006000100004
Abstract: discusses the impact of the electronic globalization and the role of library consortia in brazil as an element of information democratization. analyses the scientific journals market and describes the main recent latin-american library consortia. accomplishes a case study of a brazilian library consortium - the probe -, and its benefits to brazilian libraries and to the scientific community, as some considerations about its impact on the promotion of higher social equity.
Tuberculose multirresistente: Detec??o directa em amostras respiratórias com o método de genética molecular MTBDRplus?
Macedo,Rita; Amorim,António; Pereira,Edna;
Revista Portuguesa de Pneumologia , 2009,
Abstract: nowadays, the greatest concern of tuberculosis control programmes is the appearance of multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis. rapid determination of drug resistance in clinical samples, with mycobacterium tuberculosis complex (mtc), is the prerequisite for initiating effective chemotherapy, ensuring successful treatment of the patient and preventing further spread of drugresistant isolates. the aim of our study was to determine the sensitivity of the new mtbdrplus? assay in comparison to culture, identification and classic dst, directly from smear-positive clinical specimens. a total of 68 smear-positive sputum specimens were processed by both the classical mycobacteriological methods and the molecular assay, mtbdrplus?. mtbdrplus? assay allowed an accurate identification of mtc species by detection of the specific band in all samples, from which we also isolated and identified mtc strains by culture methods. in the samples from which we isolated susceptible strains (63.2%), wild type patterns were found using mtbdrplus ? assay. the samples from which we isolated resistant strains (36.8%) showed specific mutations associated with the correspondent resistant phenotype. our study indicated that this assay allows rapid detection of resistance, always in agreement with classic methods.
Políticas de identidade: perfil de DNA e a identidade genético-criminal Identity politics: DNA profile and the genetic-criminal identity
Helena Machado,Susana Silva,António Amorim
Análise Social , 2010,
Abstract: O DNA é visto por muitos como a “verdadeira” base da identidade humana, por se tratar de uma estrutura biológica, em princípio, única em cada indivíduo. Esta no o de “unicidade”, pilar fundamental da investiga o criminal e da genética forense, tem alimentado políticas de identidade da parte dos Estados modernos pela classifica o e armazenamento de informa o sobre “criminosos”. Neste artigo analisam-se estratégias médico-legais e burocrático-estatais de produ o da identidade “genético-criminal” relacionadas com a cria o, em Portugal, de uma base de dados forense de perfis de DNA. Discutem-se os impactos desta política de identidade na gest o, categoriza o e vigilancia de indivíduos classificados como criminosos. DNA is seen by many as the “true” basis of human identity, insofar as it is a biological structure that is, in principle, unique in each individual. This notion of “uniqueness”, a fundamental pillar of criminal investigation and forensic genetics, has fostered identity politics by modern states through the classification and storage of information about “criminals”. This article explores the alignment of science and state bureaucracy for producing the “genetic-criminal” identity in the context of the Portuguese forensic DNA database for forensic purposes. We discuss the impacts of this sort of identity politics for the management, categorization, and surveillance of individuals classified as criminals.
Consequences of primer binding-sites polymorphisms on genotyping practice  [PDF]
Estefania M. Martins, Laura Vilarinho, Sofia Esteves, Mónica Lopes-Marques, António Amorim, Luísa Azevedo
Open Journal of Genetics (OJGen) , 2011, DOI: 10.4236/ojgen.2011.12004
Abstract: Herein we investigated the effect of primer binding site polymorphisms in achieving correct genotyping when a mismatch occurs in distinct positions of the primer sequence. For that purpose primer sequences were designed in order to carry either allelic form at the 3’ end and at 3 bp, 5 bp and 7 bp apart from the 3’ end of an intronic polymorphism (rs2247836) observed in phenylalanine hydroxylase (PAH) gene. For one of the alleles annealing failure was obtained when the mismatch occurs at all the four primer-site locations. Primer sequences carrying the alternative SNP allele resulted to be less specific as the distance to the primer-3’ end was increased. Altogether, these results revealthat effects in the extension of the annealing failure is allele and mismatch-position dependent.
Comparative analyses of the Conserved Oligomeric Golgi (COG) complex in vertebrates
Rita Quental, Luísa Azevedo, Rune Matthiesen, António Amorim
BMC Evolutionary Biology , 2010, DOI: 10.1186/1471-2148-10-212
Abstract: We used protein distances and dN/dS ratios as a measure of the rate of proteins evolution. The results showed that all COG subunits are evolving under strong purifying selection, although COG1 seems to evolve faster than the remaining proteins. In addition, we also tested the expression of COG genes in 20 human tissues, and demonstrate their ubiquitous nature.COG complex has a critical role in Golgi structure and function, which, in turn, is involved in protein sorting and glycosylation. The results of this study suggest that COG subunits are evolutionary constrained to maintain the interactions between each other, as well with other partners involved in vesicular trafficking, in order to preserve both the integrity and function of the complex.Most cellular processes are carried out by multiprotein complexes that constitute important functional units in the cell [1]. This fact has motivated a number of studies aiming to investigate the structure, function and evolution of such multisubunit molecular machines [e.g., [1-4]].A cellular process in which protein complexes are known to be involved is the transport of proteins between cellular compartments (vesicular trafficking) [5,6]. Proteins synthesised in the secretory pathway are transported inside vesicles that move from the endoplasmic reticulum to the Golgi apparatus, from where polypeptides are then sorted to several cellular compartments [7]. As progression through the Golgi occurs, proteins may undergo modifications like glycosylation, a necessary step for their stability and function [8]. Several large protein complexes play an important role in the fidelity of vesicle fusion, acting as tethering factors through the formation of physical links between membranes prior to fusion [5,6,9]. One of these is the Conserved Oligomeric Golgi (COG) complex [10], which localizes at the cytoplasmic surface of the Golgi apparatus [11-14].Several studies have been performed demonstrating the involvement of COG in retrograde
Rapid identification of Aspergillus fumigatus within the section Fumigati
Rita Serrano, Leonor Gusm?o, António Amorim, Ricardo Araujo
BMC Microbiology , 2011, DOI: 10.1186/1471-2180-11-82
Abstract: A multiplex PCR was developed using prior information based on β-tubulin (βtub) and rodlet A (rodA) partial gene sequences. PCR amplification of βtub and rodA fragments resulted in a distinctive electrophoretic pattern in A. fumigatus and N. udagawae. The polymorphisms found in the smallest amplified sequence of βtub (153 bp) and rodA (103 bp) genes were then compared among and within species of this taxonomic section. βtub was able to differentiate among 13 individual species and two groups of species that included the pathogenic fungus A. lentulus. A more limited number of sequences were available for rodA; nevertheless, we were able to distinguish Aspergillus viridinutans, N. hiratsukae and N. udagawae.The assay described in the present study proved to be specific and highly reproducible, representing a fast and economic way of targeting molecular identification of the relevant mould, A. fumigatus, in clinical laboratories.Aspergillosis is the most common invasive mould disease worldwide. Recently, molecular techniques have been applied to fungal diagnosis and to the identification of species, and new fungal species that are morphologically similar to A. fumigatus have been described, authenticated and included in section Fumigati [1-3]. Therefore, this section now includes a few anamorphous Aspergillus species and teleomorphic species that are found in the genus Neosartorya [4]. The characteristics of the colonies on standard culture media are often similar to A. fumigatus, but conidia may be rather distinct. Neosartorya species produce heat-resistant ascospores [4].Misidentification of fungal species within the section Fumigati has been increasingly reported by clinical laboratories. Species, such as Aspergillus lentulus, Aspergillus viridinutans, Aspergillus fumigatiaffinis, Aspergillus fumisynnematus, Neosartorya pseudofischeri, Neosartorya hiratsukae and Neosartorya udagawae, are frequently reported as A. fumigatus [1,2,5,6]. Some of these species have been
Diversity and specificity of microsatellites within Aspergillus section Fumigati
Araujo Ricardo,Amorim António,Gusm?o Leonor
BMC Microbiology , 2012, DOI: 10.1186/1471-2180-12-154
Abstract: Background Microsatellites (or short tandem repeats, STRs) are the genetic markers of choice for studying Aspergillus fumigatus molecular epidemiology due to its reproducibility and high discrimination power. However, the specificity of these markers must be investigated in a group of isolates from closely related species. The aim of this work was to test a microsatellite-based PCR multiplex previously designed for A. fumigatus in a set of species belonging to section Fumigati, namely Aspergillus fumigatiaffinis, Aspergillus lentulus, Aspergillus novofumigatus, Aspergillus unilateralis, Aspergillus viridinutans, Neosartorya fischeri, Neosartorya hiratsukae, Neosartorya pseudofischeri and Neosartorya udagawae. Results The reference A. fumigatus strain ATCC 46645 was easily genotyped in standard conditions showing a final electrophoretic profile of 8 expected peaks corresponding to each microsatellite locus. Inversely, no peaks were observed for all other species from section Fumigati, with an exception for marker MC6b in A. unilateralis. By screening the genome sequence of Neosartorya fischeri NRRL 181, the results showed that MC3, MC6a and MC7 might be employed for N. fischeri genotyping since these markers present several repeats of each motif. The accumulation of insertions and deletions was frequently observed in the genomic regions surrounding the microsatellites, including those where the A. fumigatus primers are located. The amplification of microsatellite markers in less stringent amplification conditions resulted in a distinct electrophoretic profile for species within section Fumigati. Conclusions Therefore, the microsatellite-based PCR multiplex allow simple identification of A. fumigatus and, with a slight modification of temperature conditions, it also allows discriminating other pathogenic species within section Fumigati, particularly A. fumigatiaffinis, N. fischeri and N. udagawae.
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