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Search Results: 1 - 10 of 28222 matches for " Annie Robert "
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Beta Spaces: New Generalizations of Typically-Metric Properties
Annie Carter,Daniel Lithio,Robert Niichel,Tristan Tager
Mathematics , 2010,
Abstract: It is well-known that point-set topology (without additional structure) lacks the capacity to generalize the analytic concepts of completeness, boundedness, and other typically-metric properties. The ability of metric spaces to capture this information is tied to the fact that the topology is generated by open balls whose radii can be compared. In this paper, we construct spaces that generalize this property, called $\beta$-spaces, and show that they provide a framework for natural definitions of the above concepts. We show that $\beta$-spaces are strictly more general than uniform spaces, a common generalization of metric spaces. We then conclude by proving generalizations of several typically-metric theorems, culminating in a broader statement of the Contraction Mapping Theorem.
Management Problems of Trans-Frontier Yellow Fever Cases in Burkina Faso 2010  [PDF]
Seydou Yaro, Aline R. Ouoba, Alidou Zango, Jérémi Rouamba, Aly Drabo, Soumeya Ouangraoua, Fati Samandoulougou-Kirakoya, Jean Macq, Annie Robert, Jean Bosco Ouedraogo
Advances in Infectious Diseases (AID) , 2013, DOI: 10.4236/aid.2013.32013
Abstract:

This last decade, Burkina Faso has been confronted with yellow fever confirmed cases, mainly from Western part of the country. In 2010, National Reference laboratory of yellow fever received 970 sera of suspected cases from the 65 Health Districts of the country. We found 11 positive results by ELISA test researching specific IgM against yellow fever. An aliquot of these eleven positive sera were sent to Dakar for confirmation by sero neutralization and RT-PCR. Eight have been confirmed by regional laboratory of Pasteur Institute of Dakar and three were classified as doubtful. Confirmed cases were manly notified by Sindou (4/8) and Mangodara (3/8) Health Districts and the last one came from Nongr-masson health District situated in the central part of the country. Three out of the four confirmed cases in Sindou Health District were resident from neighboring village in Ivory Cost. Conformed cases coming from neighboring villages of Ivory Cost were difficult to manage because of the relative lack of coordination between the two health centers responsible in two different countries. The three cases were not notified to Ivory Cost Health authorities and, in addition, they didn’t benefit from the Burkina Faso response plan. The goal of this work is to present results from National Reference yellow fever laboratory in 2010 in Burkina Faso and stressing trans-frontier cases management problems in order to suggest a multinational mechanism of response to fight against this disease more effectively.

Sponge-associated bacteria of Lakshadweep coral reefs, India: resource for extracellular hydrolytic enzymes  [PDF]
Annie Feby, Shanta Nair
Advances in Bioscience and Biotechnology (ABB) , 2010, DOI: 10.4236/abb.2010.14043
Abstract: Sponges (Phylum: Porifera) is one of the major groups in the Lakshadweep coral reefs. These sponges harbor diverse bacteria with metabolic potentiality. From biodiversity to biotechnological prospecting, scientific investigations related to sponge associated microorganisms have expanded, but remain rather limited to few geographic locations. In this study, culturable bacteria associated with two demosponges viz Dysidea granulosa, Sigmadocia fibulata and the ambient water were screened for commercially important enzymes such as amylase, protease, gelatinase, lipase, deoxyribonucleic, phosphatase and urease. Amylase and phosphatase were the predominant enzymes produced by >80% of sponge-associated bacteria compared to the ambient water. Nearly 50% of the sponge-associated bacteria expressed multiple enzymatic activities (> 4) with variation in the percentage of expression of individual enzymes. More than 65% of the culturable heterotrophic bacteria associated with sponges were Gammaproteobacteria. The order Vibrionales was the main source for multiple enzyme production. Sponge associated bacteria formed more closely related clusters than the water isolates based upon their activity pattern. High recovery of sponge-associated bacteria with multiple enzymatic activities suggest that these versatile bacteria are yet unexploited potential for bioprospecting.
Stable hepatitis C virus RNA detection by RT-PCR during four days storage
Anne-Isabelle de Moreau de Gerbehaye, Monique Bodéus, Annie Robert, Yves Horsmans, Patrick Goubau
BMC Infectious Diseases , 2002, DOI: 10.1186/1471-2334-2-22
Abstract: Blood from five HCV RNA positive patients was collected in tubes with and without separator gel, centrifuged 1 or 6 hours after collection. Samples were then left 6, 24, 48, 72 or 96 h at room temperature (21.5 – 25.4°C) or at 4°C before determining their HCV RNA level using the COBAS AMPLICOR HCV MONITOR Test, vs 2.0 (Roche Diagnostic Systems).The logarithm of the HCV RNA level measurements remained within a 0.3 value of the means for 4 days at both temperatures (room temperature or 4°C).We conclude that blood samples may be collected and aliquoted within 6 h of collection and can be stored at 4°C for 72 hours as proposed by the manufacturer without significant differences in measured HCV RNA level. Our results indicate that lapses in this scheme may still yield reliable results.Both hepatitis C virus (HCV) RNA viral load and HCV genotype have been described as important for predicting the response to anti-HCV therapy. Quantification of HCV RNA in serum is used to predict treatment response and to monitor antiviral therapy. Suboptimal specimen processing and storage conditions among other factors (e.g. operator problems, reagent lot variability, etc.) may cause false-negative results in HCV RNA detection or underestimate HCV RNA load values. Since serum specimens are often transported to central laboratories for HCV RNA level determination, it was important to define conditions that preserve HCV RNA integrity. The aim of this study was to evaluate the stability of HCV RNA in serum and in clotted blood at room temperature and at 4°C for 4 days in order to optimize the standard procedures of processing and storage.We collected blood by venipuncture from five HCV RNA positive patients (2 female and 3 male; ages from 40 to 67 years) at time 0 after informed consent. All sampling and testing were done in accordance with ethical standards in Belgium and according to the guidelines of the 1975 Declaration of Helsinki. From each patient, three blood samples were collected
In Vitro Evaluation of ESE-15-ol, an Estradiol Analogue with Nanomolar Antimitotic and Carbonic Anhydrase Inhibitory Activity
Barend Andre Stander, Fourie Joubert, Chingkuang Tu, Katherine H. Sippel, Robert McKenna, Annie Margaretha Joubert
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0052205
Abstract: Antimitotic compounds are still one of the most widely used chemotherapeutic anticancer drugs in the clinic today. Given their effectiveness against cancer it is beneficial to continue enhancing these drugs. One way is to improve the bioavailability and efficacy by synthesizing derivatives that reversibly bind to carbonic anhydrase II (CAII) in red blood cells followed by a slow release into the blood circulation system. In the present study we describe the in vitro biological activity of a reduced derivative of 2-ethyl-3-O-sulphamoyl-estradiol (2EE), 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10),1?5-tetraen-17-ol(ESE-15-ol). ESE-15-ol is capable of inhibiting carbonic anhydrase activity in the nanomolar range and is selective towards a mimic of carbonic anhydrase IX when compared to the CAII isoform. Docking studies using Autodock Vina suggest that the dehydration of the D-ring plays a role towards the selectivity of ESE-15-ol to CAIX and that the binding mode of ESE-15-ol is substantially different when compared to 2EE. ESE-15-ol is able to reduce cell growth to 50% after 48 h at 50–75 nM in MCF-7, MDA-MB-231, and MCF-12A cells. The compound is the least potent against the non-tumorigenic MCF-12A cells. In vitro mechanistic studies demonstrate that the newly synthesized compound induces mitochondrial membrane depolarization, abrogates the phosphorylation status of Bcl-2 and affects gene expression of genes associated with cell death and mitosis.
Signaling Pathways of ESE-16, an Antimitotic and Anticarbonic Anhydrase Estradiol Analog, in Breast Cancer Cells
Barend Andre Stander, Fourie Joubert, Chingkuang Tu, Katherine H. Sippel, Robert McKenna, Annie Margaretha Joubert
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0053853
Abstract: The aim of this study was to characterize the in vitro action of 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10)16?-tetraene(ESE-16) on non-tumorigenic MCF-12A, tumorigenic MCF-7 and metastatic MDA-MB-231 breast cancer cells. ESE-16 is able to inhibit the activity of a carbonic anhydrase II and a mimic of carbonic anhydrase IX in the nanomolar range. Gene and protein expression studies using various techniques including gene and antibody microarrays and various flow cytometry assays yielded valuable information about the mechanism of action of ESE-16. The JNK pathway was identified as an important pathway mediating the effects of ESE-16 while the p38 stress-induced pathway is more important in MDA-MB-231 cells exposed to ESE-16. Lysosomal rupture and iron metabolism was identified as important mediators of mitochondrial membrane depolarization. Abrogation of Bcl-2 phosphorylation status as a result of ESE-16 also plays a role in inducing mitochondrial membrane depolarization. The study provides a basis for future research projects to develop the newly synthesized compound into a clinically usable anticancer agent either alone or in combination with other agents. Keywords: Antimitotic, anticarbonic anhydrase IX, apoptosis, autophagy, cell cycle arrest, Bcl-2, JNK, p38, mitochondrial membrane depolarization, flow cytometry, gene expression and protein microarray, anticancer.
Spatial and Spectral Modeling of the Gamma-ray Distribution in the Large Magellanic Cloud
Gary Foreman,You-Hua Chu,Robert Gruendl,Annie Hughes,Brian Fields,Paul Ricker
Physics , 2015, DOI: 10.1088/0004-637X/808/1/44
Abstract: We perform spatial and spectral analyses of the Large Magellanic Cloud (LMC) gamma-ray emission collected over 66 months by the Fermi Gamma-ray Space Telescope. In our spatial analysis, we model the LMC cosmic-ray distribution and gamma-ray production using observed maps of the LMC interstellar medium, star formation history, interstellar radiation field, and synchrotron emission. We use bootstrapping of the data to quantify the robustness of spatial model performance. We model the LMC gamma-ray spectrum using fitting functions derived from the physics of $\pi^0$ decay, bremsstrahlung, and inverse Compton scattering. We find the integrated gamma-ray flux of the LMC from 200 MeV to 20 GeV to be $1.38 \pm 0.02 \times 10^{-7}$ ph cm$^{-2}$ s$^{-1}$, of which we attribute about 10% to inverse Compton scattering and 40% to bremsstrahlung. From our work, we conclude that the spectral index of the LMC cosmic-ray proton population is 2.4$\pm$0.2, and we find that cosmic-ray energy loss through gamma-ray production is concentrated within a few hundred pc of acceleration sites. Assuming cosmic-ray energy equipartition with magnetic fields, we estimate LMC cosmic rays encounter an average magnetic field strength ~3 $\mu$G.
Neisseria meningitis serogroup X outbreak in Burkina Faso, 2009-2010  [PDF]
Seydou Yaro, Aly Drabo, Soumeya Ouangraoua, Fati Kirakoya-Samadoulougou, Judith Mueller, Oumar Sanou, Haoua Tall, Phillip Jaillard, Berthe-Marie Njanpop-Lafourcade, Jean Macq, Annie Robert, Jean Bosco Ouedraogo
Open Journal of Internal Medicine (OJIM) , 2012, DOI: 10.4236/ojim.2012.22010
Abstract: Background: Centre MURAZ of Bobo-Dioulasso (Burkina Faso) organized in 2009 and 2010 a system of Cerobro-Spinal Fluid (CSF) collection in eight pilot Districts as an initial step for the future Ministry of Health’s led strategy of individual surveillance in a context of meningococcal conjugate A vaccine introduction. Methods: CSF samples were analyzed with Polymerase Chain Reaction (PCR). This allowed for meningitis etiologies dynamics studies in the pilot Districts. Results: Because of geographical difficulties and lack of means, less than 40% of suspected cases had their CSF analyzed at PCR reference laboratory. In 2009, among confirmed cases at reference laboratory, Sp (Streptococcus pneumonia), NmA (Neisseria meningitis A) and Hib (Hemophilus influenzae b) were responsible respectively for 90%, 6.6% and 4.4% of cases. In 2010, serogroup distribution among confirmed cases was: Sp 62.7%, NmX 32.2% and NmA 5.1%. Sp which was continuously present in Burkina Faso takes more significant proportions, just as serogroup X which until there was sporadically encountered. The attack rates of NmX were tree to twelve times higher than for NmA in the two Districts where NmX has been notified. Conclusion: As a consequence of such results, efforts must be maintained in epidemiologic surveillance field and in reinforcement of laboratory capacities. Fast care should be guaranteed to patients with adequate antibiotics according to country national guideline and chemoprophylaxis measures should be undertaken among contacts of patients to prevent secondary cases. A plea must be made on one hand for pneumococcal vaccine introduction in Burkina Faso and on other hand towards manufacturers for taking into account serogroup X into meningococcal polyvalent vaccine composition. With this polyvalent vaccine including serougruop X, we suggested to conduct periodically mass campaign vaccination of people before the beginning of meningitis epidemiological season.
Review: Gram Negative Bacteria in Brewing  [PDF]
Paradh Ashtavinayak, Hill Annie Elizabeth
Advances in Microbiology (AiM) , 2016, DOI: 10.4236/aim.2016.63020
Abstract: Gram negative aerobic bacteria such as Acetic Acid Bacteria, which include Acetobacter and Gluconobacter, have historically caused significant problems to brewers. Although incidences of spoilage have recently reduced as a result of improvements in beer packaging, these bacteria are still a concern in dispense systems in pub breweries, public houses and cask conditioned beers. Gram negative facultative bacteria of the genus Zymomonas can spoil primed cask conditioned beer and cider. There is a wide range of Enterobacteraeceace which are found within brewery environments and they serve as indicator microorganisms for hygiene and sanitation. Gram negative strictly anaerobic bacteria such as Pectinatus and Megasphaera have recently emerged as a significant threat due to the improvement in reduction of oxygen levels in beer and an increase in production of unpasteurised beer. Pectinatus and Megasphaera are sensitive to routine cleaning agents used in breweries, but they can survive and proliferate in biofilms eventually causing spoilage of beer. This review focuses on Gram negative aerobic, facultative anaerobic and strictly anaerobic brewery related spoilage bacteria.
Benjamin & Schmitt: uma arqueologia da exce??o
Dymetman, Annie;
Lua Nova: Revista de Cultura e Política , 2001, DOI: 10.1590/S0102-64452001000200006
Abstract: situated at opposed poles of the ideological and cultural life in the twenties and thirties of the last century. walter benjamin and carl schmitt are protagonists in a complex play of affinity and repulsion on the level of their basic conceptions, which are built around the theme of excess. the author proposes an hermeneutic of excess.
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