oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 163 )

2018 ( 838 )

2017 ( 766 )

2016 ( 1130 )

Custom range...

Search Results: 1 - 10 of 470673 matches for " Angela María;Restrepo "
All listed articles are free for downloading (OA Articles)
Page 1 /470673
Display every page Item
RNAi technology targeting PbGP43 and PbP27 in Paracoccidioides brasiliensis  [PDF]
Isaura Torres Gómez, Orville Hernandez Ruiz, Jose F. Mu?oz, Ana María Garcia, Angela Restrepo, Juan G. McEwen
Open Journal of Genetics (OJGen) , 2013, DOI: 10.4236/ojgen.2013.32A2001
Abstract:

Efficient technologies for gene silencing would be important to carry out functional analysis with P. brasiliensis genes, as well as for a better understanding of the biology and pathogenesis of this pathogenic fungus. Due to the fact that homologous recombination is unusual in P. brasiliensis, the development of knockout isolates is currently non-feasible. The goal of this work was to assess RNA interference (RNAi) technology as an alternative tool for gene silencing previously employed successfully in H. capsulatum. For this purpose, we built different inverted repeat transgenic hairpin constructs to down-regulate the PbGP43 and PbP27 genes known to codify for two fungal immunogenic proteins that elicit a strong immune response during experimental paracoccidioidomycosis. Using the RNAi strategy, a reduction in the mRNA levels of the PbGP43 and PbP27 genes was observed during the first 20 days after selection; however, in the transformed yeast cells, the gene silencing status proved non-stable through the assay. We demonstrated that electrotransformation was suitable to transform P. brasiliensis yeast cells and integrate the hairpin constructions; nonetheless, gene silencing was not stable along the experimental time. A detailed analysis of the underlying molecular RNAi machinery may provide further insights into the intracellular mechanism that governs this reverse genetic tool.

Hipocalcemia por dependencia tipo I de vitamina D en un paciente con nefrolitiasis por ácido úrico
César A,Restrepo; Benjumea,Angela María;
Acta Medica Colombiana , 2010,
Abstract: we discuss the case of a 37-year-old man with hyperuricemia, bilateral nephrolithiasis, and mineral metabolic disorder characterized by hypocalcemia, hypophosphatemia, and hypocalciuria without phosphaturia. the diseased had developed over 10 years without an accurate diagnosis, and had responded poorly to medical management. after clinical analyses and laboratory workup, vitamin d dependency type i was diagnosed (acta med colomb 2010; 35: 96-99).
Hipocalcemia por dependencia tipo I de vitamina D en un paciente con nefrolitiasis por ácido úrico Hypocalcemia due to vitamin D dependency type I in a patient with uric acid nephropathy
Restrepo César A,Angela María Benjumea
Acta Medica Colombiana , 2010,
Abstract: Se discute el caso de un paciente hombre de 37 a os con hiperuricemia, nefrolitiasis múltiple bilateral, y trastorno metabólico mineral caracterizado por hipocalcemia, hipofosfatemia, hipocalciuria y normofosfaturia de 10 a os de evolución, con pobre respuesta al manejo médico instaurado y sin diagnóstico preciso. Tras análisis clínicos y apoyo en exámenes de laboratorio se evidencia dependencia tipo I a la vitamina D (Acta Med Colomb 2010; 35: 96-99) We discuss the case of a 37-year-old man with hyperuricemia, bilateral nephrolithiasis, and mineral metabolic disorder characterized by hypocalcemia, hypophosphatemia, and hypocalciuria without phosphaturia. The diseased had developed over 10 years without an accurate diagnosis, and had responded poorly to medical management. After clinical analyses and laboratory workup, vitamin D dependency type I was diagnosed (Acta Med Colomb 2010; 35: 96-99).
TRANSVERSALIDAD CURRICULAR ORIENTADA A CREACIóN DE EMPRESAS Facultad de Ingeniería Industrial
SANDRA ESTRADA MEJíA,ANGELA MARíA LANZAS,LUZ STELLA RESTREPO
Scientia Et Technica , 2004,
Abstract: El presente artículo se fundamenta en la importancia que tiene la creación de empresas para el beneficio de la comunidad. Partiendo del diagnóstico de la situación actual del currículo de la Facultad de Ingeniería Industrial, se dise o una propuesta que conlleva a la formación integral de los estudiantes con espíritu y mentalidad empresarial.
Sensibilidad a fluconazol y voriconazol de aislamientos de Candida spp., obtenidos de mucosa oral de pacientes con sida
Gutiérrez,Carolina; de Bedout,Catalina; Tobón,Angela María; Cano,Luz Elena; Arango,Myrtha; Tabares,Angela María; Restrepo,Angela;
Infectio , 2007,
Abstract: abstracts the susceptibilities to fluconazol (fcz) and voriconazol (vcz) susceptibility of candida spp. isolates, obtained from the oral mucosa of 54 aids patients hospitalized in hospital la maría (medellín, colombia) were assesed. additionally, the yeast species were identified to species. patients were all adults (mean, 40,5 years old, range 23 to 56) and most (77,8%) were males. candida spp. was isolated from 40 of them (71.1 %) with 6 (11,1%) showing more than one species. the species identified were c. albicans., c. krusei, c. tropicalis, c. parapsilosis and c. glabrata. in vitro agar diffusion susceptibility testing was done according to the clsi m44p specifications. most of the candida species (72.9%) were susceptible to fluconazole with 6,3% susceptible-dose-dependant (sdd) and 20.8% resistant. voriconazole susceptibility was higher (89,6%) with 8,3% sdd and 2,1% resistant. resistance was recorded for c. albicans and c. krusei isolates. the results demonstrate the effectiveness of both antifungals with voriconazole performing better. the importance of the in vitro susceptibility tests in hiv/aids patients harboring resistant species was demonstrated and indicated the need to classify all candida isolate al candida isolates to the species level.
Identification of Paracoccidioides brasiliensis in adrenal glands biopsies of two patients with paracoccidioidomycosis and adrenal insufficiency
Agudelo, Carlos Andrés;Mu?oz, Carolina;Ramírez, Alex;Gutierrez, Jonhairo;Velez, Santiago;Perez, Juan Camilo;Velez, Alejandro;Tobon, Angela María;Restrepo, Angela;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2009, DOI: 10.1590/S0036-46652009000100008
Abstract: the authors report two cases of adrenal insufficiency secondary to infiltration of the adrenal glands by paracoccidioides brasiliensis. the first patient had been treated for a chronic multifocal form of paracoccidiodomycosis 11 years ago. the diagnosis of the mycosis was done simultaneous with that of the adrenal insufficiency in the second patient. in both patients the diagnosis was done by direct visualization of fungus in adrenal biopsies. they were treated with hormonal supplements and itraconazol by 12 and six months, without relapses during the follow-up period.
El canal del crédito bancario en Colombia: 1995-2005. Una aproximación mediante modelos de umbral
Restrepo,María Isabel;
Lecturas de Economía , 2007,
Abstract: the bank credit channel, which amplifies the effects of the traditional channel of monetary policy, emphasizes on the structure and frictions of financial markets as determinants of aggregate spending. this paper aims at analyze and verify the existence of the bank credit channel in colombia estimating a model proposed by gibson (1997) which uses threshold regressions as a way to determine the impact of monetary policy on aggregate demand. results do not allow dismissing the existence of this transmission mechanism in colombia during the analyzed period, although it seems to operate only through contractionary monetary policy.
Tratamiento. Leishmania
Adriana María Restrepo
Biomédica , 2011,
Abstract:
El canal del crédito bancario en Colombia: 1995-2005. Una aproximación mediante modelos de umbral
María Isabel Restrepo,Diana Constanza Restrepo
Lecturas de Economía , 2007,
Abstract: The Bank Credit Channel, which amplifies the effects of the traditionalchannel of monetary policy, emphasizes on the structure and frictions of financial marketsas determinants of aggregate spending. This paper aims at analyze and verify the existenceof the bank credit channel in Colombia estimating a model proposed by Gibson (1997)which uses threshold regressions as a way to determine the impact of monetary policyon aggregate demand. Results do not allow dismissing the existence of this transmissionmechanism in Colombia during the analyzed period, although it seems to operate onlythrough contractionary monetary policy.
Moneda y banca en una economía aurífera. La Región de Antioquia (Colombia), 1850-1890
Botero Restrepo, María Mercedes;
América Latina en la historia económica , 2011,
Abstract: in the 1850s, antioquia started showing significant commercial development resulting mostly from gold-mining expansion. new mining sites generated new markets and trade networks that joined small localities with cities and foreign markets. based on the archives of commercial houses of the time, this essay contributes to studies of 19th century monetary issues, describing different forms of money placed into circulation that facilitated the exchange of goods and services in this mining economy.
Page 1 /470673
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.