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Search Results: 1 - 10 of 358394 matches for " Angélica P;Lezama-Dávila "
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Serotypes of Vibrio cholerae Non-O1 Isolated from Water Supplies for Human Consumption in Campeche, México and their Antibiotic Susceptibility Pattern
Isaac-Márquez, Angélica P;Lezama-Dávila, Claudio M;Eslava- Campos, Carlos;Navarro-Oca?a, Armando;Cravioto-Quintana, Alejandro;
Memórias do Instituto Oswaldo Cruz , 1998, DOI: 10.1590/S0074-02761998000100004
Abstract: the presence of vibrio cholerae non-o1 in water supplies for human consumption in the city of campeche and rural locality of bécal was investigated. v. cholerae non-o1 was detected in 5.9% of the samples obtained in deep pools of campeche. studies conducted in bécal and neighbourhood of morelos in campeche indicated that collected samples harbored v. cholerae non-o1 in 31.5% and 8.7% respectively. there was a particular pattern of distribution of v. cholerae non-o1 serotypes among different studied regions. accordingly, v. cholerae non-o1 serotype o14 predominated in the deep pools of campeche and together with v. cholerae non-o1, o155 were preferentially founds in samples taken from intradomiciliary faucets in the neighbourhood of morelos. samples from bécal predominantly presented the serotype o112. 60% and 53.8% of all studied strains of v. cholerae non-o1 proved to be resistant to ampicillin and carbenicillin. 3.1%, 7.7% and 6.2% presented resistant to doxycycline, trimethoprim-sulfamethoxazole and erythromycin respectively. the study showed the necessity of performing a strong epidemiologic surveillance for emergence and distribution of v. cholerae non-o1
Serotypes of Vibrio cholerae Non-O1 Isolated from Water Supplies for Human Consumption in Campeche, México and their Antibiotic Susceptibility Pattern
Isaac-Márquez Angélica P,Lezama-Dávila Claudio M,Eslava- Campos Carlos,Navarro-Oca?a Armando
Memórias do Instituto Oswaldo Cruz , 1998,
Abstract: The presence of Vibrio cholerae non-O1 in water supplies for human consumption in the city of Campeche and rural locality of Bécal was investigated. V. cholerae non-O1 was detected in 5.9% of the samples obtained in deep pools of Campeche. Studies conducted in Bécal and neighbourhood of Morelos in Campeche indicated that collected samples harbored V. cholerae non-O1 in 31.5% and 8.7% respectively. There was a particular pattern of distribution of V. cholerae non-O1 serotypes among different studied regions. Accordingly, V. cholerae non-O1 serotype O14 predominated in the deep pools of Campeche and together with V. cholerae non-O1, O155 were preferentially founds in samples taken from intradomiciliary faucets in the neighbourhood of Morelos. Samples from Bécal predominantly presented the serotype O112. 60% and 53.8% of all studied strains of V. cholerae non-O1 proved to be resistant to ampicillin and carbenicillin. 3.1%, 7.7% and 6.2% presented resistant to doxycycline, trimethoprim-sulfamethoxazole and erythromycin respectively. The study showed the necessity of performing a strong epidemiologic surveillance for emergence and distribution of V. cholerae non-O1
Detection of pathogenic bacteria in skin lesions of patients with chiclero's ulcer: reluctant response to antimonial treatment
Isaac-Márquez, Angélica Patricia;Lezama-Dávila, Claudio Manuel;
Memórias do Instituto Oswaldo Cruz , 2003, DOI: 10.1590/S0074-02762003000800021
Abstract: we investigated the bacterial flora present in skin lesions of patients with chiclero's ulcer from the yucatan peninsula of mexico using conventional culture methods (11 patients), and an immunocolorimetric detection of pathogenic streptococcus pyogenes (15 patients). prevalence of bacteria isolated by culture methods was 90.9% (10/11). we cultured, from chiclero's ulcers (60%), pathogenic bacterial such as staphylococcus aureus (20%), s. pyogenes (1.6%), pseudomonas aeruginosa (1.6%), morganella morganii (1.6%), and opportunist pathogenic bacteria such as klebsiella spp. (20.0%), enterobacter spp. (20%), and enterococcus spp. (20%). we also cultured coagulase-negative staphylococci in 40% (4/10) of the remaining patients. micrococcus spp. and coagulase-negative staphylococci constituted the bacterial genuses more frequently isolated in the normal skin of patients with chiclero's ulcer and healthy individuals used as controls. we also undertook another study to find out the presence of s. pyogenes by an immunocolorimetric assay. this study indicated that 60% (9/15) of the ulcerated lesions, but not normal controls, were contaminated with s. pyogenes. importantly, individuals with purulent secretion and holding concomitant infections with s. pyogenes, s. aureus, p. aeruginosa, m. morganii, and e. durans took longer to heal leishmania (l.) mexicana infections treated with antimonial drugs. our results suggest the need to eliminate bacterial purulent infections, by antibiotic treatment, before starting antimonial administration to patients with chiclero's ulcer.
Detection of pathogenic bacteria in skin lesions of patients with chiclero's ulcer: reluctant response to antimonial treatment
Isaac-Márquez Angélica Patricia,Lezama-Dávila Claudio Manuel
Memórias do Instituto Oswaldo Cruz , 2003,
Abstract: We investigated the bacterial flora present in skin lesions of patients with chiclero's ulcer from the Yucatan peninsula of Mexico using conventional culture methods (11 patients), and an immunocolorimetric detection of pathogenic Streptococcus pyogenes (15 patients). Prevalence of bacteria isolated by culture methods was 90.9% (10/11). We cultured, from chiclero's ulcers (60%), pathogenic bacterial such as Staphylococcus aureus (20%), S. pyogenes (1.6%), Pseudomonas aeruginosa (1.6%), Morganella morganii (1.6%), and opportunist pathogenic bacteria such as Klebsiella spp. (20.0%), Enterobacter spp. (20%), and Enterococcus spp. (20%). We also cultured coagulase-negative staphylococci in 40% (4/10) of the remaining patients. Micrococcus spp. and coagulase-negative staphylococci constituted the bacterial genuses more frequently isolated in the normal skin of patients with chiclero's ulcer and healthy individuals used as controls. We also undertook another study to find out the presence of S. pyogenes by an immunocolorimetric assay. This study indicated that 60% (9/15) of the ulcerated lesions, but not normal controls, were contaminated with S. pyogenes. Importantly, individuals with purulent secretion and holding concomitant infections with S. pyogenes, S. aureus, P. aeruginosa, M. morganii, and E. durans took longer to heal Leishmania (L.) mexicana infections treated with antimonial drugs. Our results suggest the need to eliminate bacterial purulent infections, by antibiotic treatment, before starting antimonial administration to patients with chiclero's ulcer.
CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection
Lezama-Dávila, C. M;Gallagher, G;
Memórias do Instituto Oswaldo Cruz , 1995, DOI: 10.1590/S0074-02761995000100012
Abstract: we studied the role of cd4+, cd8+, cd4- cd8- t cells and igg anti-leishmania after infection or vaccination in the cba/ca mouse. mice were either infected with l. m. mexicana promastigotes or vaccinated with parasite-membrane antigens incorporated into liposomes. successfully vaccinated mice were used as cell-donors in adoptive transfer experiments. naive, syngeneic recipients received highly-enriched cd4+, cd8+ or cd4- cd8- t cells from those two set of donors and challenged with live parasites. our results showed that, both cd4+ and cd8+ t cells from infected or vaccinated donors conferred significant disease-resistance to naive recipients. in addition, adoptive transfer of cd4- cd8- t cells from vaccinated donors significantly delayed lesion growth in recipient mice. we concluded that vaccination of cba mice correlates with the induction of protective cd4+, cd8+ and cd4- cd8- t cells and the synthesis of igg anti-leishmania.
Frecuencia y características del consentimiento informado en ensayos clínicos
Dora Angélica Corrales-Portales,Luís Cruzado-Dávila,Juan Huamán-Saavedra
CIMEL. Ciencia e Investigación Médica Estudiantil Latinoamericana , 2007,
Abstract: Objetivo: determinar la frecuencia y características del consentimiento informado en ensayos clínicos aleatorizados. Dise o: estudio transversal y descriptivo. Lugar: Hemeroteca de la Facultad de Medicina de la Universidad Nacional de Trujillo. Participantes: todos los ensayos clínicos aleatorizados (tesis para optar el grado Bachiller de Medicina, tesis para optar el grado de Especialista y trabajos de investigación docente) registrados desde 1994 a 2006. Intervenciones: fichas de registro elaboradas por los autores para la obtención de datos. Principales medidas de resultado: análisis en frecuencias absolutas y relativas de consentimiento informado explícito, simple mención del consentimiento informado y no referencia del consentimiento informado. Resultados: se encontró que 42,3 % de los 314 trabajos evaluados tuvo consentimiento informado explicito: 43% de las tesis de Bachiller, 61.4% de las tesis de Especialista y 13,7 % de trabajos de Investigación Docente (p<0,0001). Respecto a los criterios: 80% refirió el título de la investigación (p=0,003) y 97% refirió la participación voluntaria, los demás criterios aparecen en menos de 40%. Conclusiones: el consentimiento informado en ensayos clínicos aleatorizados tiene una frecuencia moderada de aparición, con una escasa presencia en la investigación docente; mientras que la mayoría de los criterios observados en el consentimiento informado explicito son muy poco frecuentes
Influência de argilas pilarizadas na decomposi??o catalítica do óleo de andiroba
Guerra, D. L.;Lemos, V. P.;Angélica, R. S.;Airoldi, C.;
Eclética Química , 2007, DOI: 10.1590/S0100-46702007000400003
Abstract: smectite-bearing clay samples from pará state, amazon region, brazil, were used for pillaring process in the present study. the natural and pillarized matrices were characterized using xrd and textural analysis using nitrogen adsorption-desorption isotherms. the ions of intercalation (al13, ti, zr) were obtained through chemical reactions of alcl36h2o/ naoh, titanium ethoxide /hcl, zirconium acetate /hcl solutions. the results showed that the pillarization process increases the basal spacing of the natural clay from 15.6 to 20.64 ? and the superficial area from 44 to 358 m2/g (zr-pilc). the thermal stability of the natural clay was improved by the pillaring procedure. the resulting materials were submitted to catalytic process of decomposition of andiroba oil in a fix bed reactor at 673 ± 1 k. the catalytic activity was determined by the product composition resulting from the chemical reactions. the physicochemical parameters of the catalysts were obtained using xrd, ftir and textural analysis. the pillared clays showed higher br?nsted acidity, with higher concentration in aromatic hydrocarbons and small concentration of aliphatic hydrocarbons.
Aplica??o de Al-PILC na adsor??o de Cu2+, Ni2+e Co2+ utilizando modelos físico-químicos de adsor??o
Guerra, D. L.;Airoldi, C.;Lemos, V. P.;Angélica, R. S.;Viana, R. R.;
Eclética Química , 2007, DOI: 10.1590/S0100-46702007000300008
Abstract: smectite sample from the serra de maicuru area (pará state, northern brazil, amazon region) were used for the pillaring process with al13. aluminum pillared clay (al-pilc) was characterized by xrd, sem and eds. the textural analysis using nitrogen adsorption-desorption isotherms. the aim of this paper is to study in how al-pilc adsorb heavy metals. the adsorption of cu2+, ni2+, co2+ ions from aqueous solution in room temperature by al-pilc have been carried out. the langmuir, freundlich and temkin adsorption isotherm models have been applied to fit the experimental data, with the first model well adjusted with r = 0.999. the one-surface langmuir equation provided the best fit to the data. the freundlich equation presented limitations in rises concentrations, but acceptable values of parameters were obtained (kf and n) with the use of the three models. the parameters were used to calculate the amount adsorbed nf, a function constant cs.
Influência da raz?o Al/argila no processo de pilariza??o de esmectita
Guerra, D. L.;Lemos, V. P.;Angélica, R. S.;Airoldi, C.;
Ceramica , 2006, DOI: 10.1590/S0366-69132006000300014
Abstract: smectite-type clays from the state of pará, brazil, were characterized by xrd, 27al mas nmr, ftir and textural analysis by nitrogen adsorpition-desorption isotherms. in the production of pillared clays, the al13 ion (keggin's ion) was used. this ion was obtained by the reaction of alcl36h2o/naoh solutions with oh/al=2 molar ratio and with al/clay ratios of 5, 10, 15, 20, 25 meq/g of clay, intercalated at 25 oc during 3 h and calcined at 450 oc (adequate temperature for calcination). the results showed that the pillarization process increased the basal spaces of the natural clay from 14.02 to 18.84 ? and the surface area from 44.30 to 198.03 m2/g (al/g of clay = 25 meq/g of clay, ph=4). materials prepared with different al/g clay ratio showed the maximum al incorporation for al/g of clay = 25 meq/g of clay. the thermal stability of the natural clay was improved by the pillaring procedure.
Influência do método de síntese no processo de pilariza??o com titanio de uma esmectita da regi?o amaz?nica
Guerra, D. L.;Lemos, V. P.;Angélica, R.;Airoldi, C.;
Ceramica , 2006, DOI: 10.1590/S0366-69132006000400003
Abstract: smectite clays of the state of acre, brazil, were used for the pillarization process. the natural and pillarized matrices were characterized by x-ray diffraction, fourier transform infrared spectroscopy (ftir), thermal analysis, scanning electron microscopy, energy dispersive spectroscopy and textural analysis. the titanium pillarized solutions ti(oc2h5)4 and ticl4 were analyzed by ftir. the intercalation ion was obtained by reacting ti(oc2h5)4 with hcl (hcl/ti ~ 1) and ticl4 with ethanol (ti/clay ~ 25 mmol ti/g). the notronite intercalation was carried out using two methods: with dropwise hydrochloric acid in titanium ethoxide solution, and using titanium tetrachloride, kept under vigorous stirring at 25 °c for 3 h and calcination at 450 °c and 600 °c. the x-ray diffraction results of the pillarization process show an increase (15.30 ? to 18.76 ?) in the basal spacing of the natural clay and in the specific surface area (44.37 m2/g to 188.72 m2/g) the thermal stability of the natural clay was improved by the pillarization procedure.
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