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Search Results: 1 - 10 of 20023 matches for " André Nepomuceno;Buso "
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Shoot tip culture and thermotherapy for recovering virus-free plants of garlic
Torres, Antonio Carlos;Fajardo, Thor Vinícius;Dusi, André Nepomuceno;Resende, Renato de Oliveira;Buso, José Amauri;
Horticultura Brasileira , 2000, DOI: 10.1590/S0102-05362000000300010
Abstract: garlic shoot tip culture associated with dry heat thermotherapy (cloves exposed to 37°c for 35 days) were essential for recovering virus free plants of the cv amarante. in this condition 70% of the explants developed in vitro and produced plants. a total of 77% of those plants was virus free when indexed by isem, which resulted in a final index of 54% of virus free plants from treated cloves. the percentage of regeneration decreased to 20% as the temperature increased up to 40°c. however 90% of those plants were virus free, leading to a final index of 18% virus free plants out of treated cloves.
Detection of three Allexivirus species infecting garlic in Brazil
Melo Filho, Péricles de Albuquerque;Nagata, Tatsuya;Dusi, André Nepomuceno;Buso, José Amauri;Torres, Antonio Carlos;Eiras, Marcelo;Resende, Renato de Oliveira;
Pesquisa Agropecuária Brasileira , 2004, DOI: 10.1590/S0100-204X2004000800002
Abstract: garlic viruses often occur in mixed infections under field conditions. in this study, garlic samples collected in three geographical areas of brazil were tested by dot-elisa for the detection of allexiviruses using monoclonal specific antibodies to detect garlic virus a (garv-a), garlic virus b (garv-b), garlic virus c (garv-c) and a polyclonal antiserum able to detect the three virus species mentioned plus garlic virus d (garv-d). the detected viruses were biologically isolated by successive passages through chenopodium quinoa. reverse transcriptase polimerase chain reaction (rt-pcr) was performed using primers designed from specific regions of the coat protein genes of japanese allexiviruses available in the genetic bank of national center of biotechnology information (ncbi). by these procedures, individual garlic virus genomes were isolated and sequenced. the nucleotide and amino acid sequence analysis and the one with serological data revealed the presence of three distinct allexiviruses garv-c, garv-d and a recently described allexivirus, named garlic mite-borne filamentous virus (garmbfv), in brazil.
A modernidade da História nas raízes do humanismo
Luíz André Nepomuceno
OPSIS : Revista do Departamento de História e Ciências Sociais , 2005,
Abstract: Je voudrais proposer une recherche sur naissance moderne de les intérêts par l’Histoire justement aux racines du premier Humanisme. Il est net que, pour que l’homme accorde son lieu dans l’Histoire, il faut qu’il se rend digne, qu’il accorde à soi même um r le invulnérable d’agent qui transform c’est-à-dire, l’être humain (soit quelque temps) quin e concèdes, pas à soi même lê droit d’intervenir à la conception de l’univers n’a pas également entendement de la notion de l’Histoire. / Gostaria de propor uma investiga o sobre o nascedouro moderno dos interesses pela História justamente nas raízes do primeiro Humanismo. Está claro que, para que o homem conceda o seu lugar na História, é preciso que ele se dignifique, que ele conceda a si um papel invulnerável de agente transformador, e n o apenas de agente entendedor, ou seja, o ser humano (em qualquer tempo que seja) que n o concede a si o direito de intervir na concep o do universo n o tem igualmente entendimento da no o de História.
Da alma e das letras: a eloqüência como projeto humanista
Luís André Nepomuceno
Caligrama : Revista de Estudos Romanicos , 2005,
Abstract: Resumo: No presente artigo, prop e-se uma análise da carta Fam. I 9 (dos Rerum familiarium libri), de Francesco Petrarca, que coloca em evidência algumas das considera es fundamentais do humanista italiano sobre a arte da eloqüência; e discute-se ainda a composi o de alguns procedimentos utilizados por Petrarca na constru o de uma rela o entre a retórica e as virtudes do espírito. Palavras-chave: Francesco Petrarca; Rerum Familiarium Libri; eloqüência; retórica. Abstract: In questo articolo si propone un’analisi dell’epistola Fam. I 9 (dei Rerum Familiarium Libri), di Francesco Petrarca, che mette in evidenza alcune delle considerazioni fondamentali dell’umanista italiano sull’arte dell’eloquenza; e si discute anche la composizione di procedimenti utilizzati da Petrarca nella costruzione di una relazione fra la retorica e le virtù dell’animo. Parole chiave: Francesco Petrarca; Rerum Familiarium Libri; eloquenza; retorica.Keywords: Francesco Petrarca; Rerum Familiarium Libri; eloquence; rhetorica.
De mim mesmo sou inimigo : exílio e saudade na écloga II de Bernardim Ribeiro
Luís André Nepomuceno
Caligrama : Revista de Estudos Romanicos , 2011,
Abstract: Resumo: O presente estudo analisa algumas quest es históricas e filosóficas envolvidas na écloga II (“Jano e Franco”) de Bernardim Ribeiro, incluída na sua editio princeps publicada em 1554, em Ferrara, por iniciativa de Abra o Usque, judeu português exilado na Itália. Até o séc. XIX, as éclogas tinham sido vistas como poesia de temática romanesca e amorosa, e apenas recentemente identificadas (juntamente com a Menina e mo a, romance pastoril do mesmo autor) como obra de resistência judaica. Este trabalho pretende identificar as éclogas de Bernardim Ribeiro, em especial a “Jano e Franco”, com as obras consolatórias sefarditas t o em voga na Península Ibérica do séc. XVI, buscando sua identidade literária e política especialmente na Consola o às tribula es de Israel, de Samuel Usque, ou em livros referenciais da cabala, como o Zohar. Palavras-chave: Renascimento português; judaísmo; platonismo. Resumen: El presente estudio analiza algunas cuestiones históricas y filosóficas involucradas en la égloga II (“Jano e Franco”) de Bernardim Ribeiro, incluida en su editio princeps publicada en 1554, en Ferrara, por iniciativa de Abra o Usque, portugués judío exiliado en Italia. Hasta el siglo 19, las églogas habían sido vistas como poesía de temática romanesca y amorosa, y sólo recientemente identificadas (juntamente con Menina e mo a, romance pastoril del mismo autor) como obra de resistencia judaica. Este trabajo pretende identificar las églogas de Bernardim Ribeiro, en especial “Jano e Franco” con la obras consolatorias sefarditas tan en boga en la Península Ibérica del siglo 16, buscando su herencia literaria y política especialmente en Consola o às tribula es de Israel, de Samuel Usque, o en libros referenciales de la cabala, como el Zohar. Palabras-clave: Renacimiento portugués; judaísmo; platonismo. Keywords: Portuguese Renaissance; Judaism; Platonism.
Terza partita. La musica nell’età della tecnica – un madrigale
Nicola Buso
Musica e Tecnologia , 2011,
Abstract: in the electroacoustic music field the writing grow weak; the action, the listening, the body memory come out; the writing become theatrical action in the collaborative live coding praxis over network: the technological action reveal itself like the root of language, of thought, of culture, like the essence of man, where history accomplish.
Spread of viruses in garlic fields cultivated under different agricultural production systems in Brazil
Fayad-André, Michelle de S.;Dusi, André Nepomuceno;Resende, Renato Oliveira;
Tropical Plant Pathology , 2011, DOI: 10.1590/S1982-56762011000600001
Abstract: samples from four regions, representing 76% of the garlic growing area in brazil, were tested by rt-pcr for the presence of onion yellow dwarf virus (oydv), leek yellow stripe virus (lysv), garlic common latent virus (gclv), garlic virus c (garv-c), garlic virus d (garv-d) and garlic mite-borne filamentous virus (garmbfv). the samples (352 bulbs) represented five agricultural systems: traditional common garlic (cg), virus-free common garlic (vfcg), non-vernalized noble garlic (nvngsg) and vernalized noble garlic cultivated by small growers (vngsg), and vernalized noble garlic adopted by major growers (vngmg). multiple infections were detected in 22% of the samples. potyvirus species were present in all regions. lysv prevailed over oydv while the carlavirus gclv was less prevalent. garv-c and garmbfv were the most prevalent among the allexivirus species. the nvng production system had a higher prevalence of lysv and garv-c. the cg production system, that uses less technology, had the highest prevalence for all species, especially lysv that prevailed in 94% of the samples. overall, the regions with higher technological input employing better quality seeds had the lowest viral prevalence for all species. this monitoring provides information to establish a strategy to raise the phytosanitary quality and the national productivity of garlic.
Perfil epidemiológico da raiva na regi?o Noroeste do Estado de S?o Paulo no período de 1993 a 2007
Queiroz, Luzia Helena;Carvalho, Cristiano de;Buso, Daniel Sartori;Ferrari, Clara Isabel de Lucca;Pedro, Wagner André;
Revista da Sociedade Brasileira de Medicina Tropical , 2009, DOI: 10.1590/S0037-86822009000100003
Abstract: the epidemiology of animal rabies in the region of ara?atuba, in the northwest of s?o paulo state, from 1993 to 2007, is described according to the results from diagnoses made at laboratories in the region, using the fluorescent antibody and mouse inoculation tests. out of 10,579 samples analyzed, 4.9% were positive (518/10,579). dogs accounted for 67% of the cases (346/518) and these occurred between 1993 and 1997. among the other positive samples, 16% (84/518) were in cattle and 9.7% (50/518) were in bats. among the 42 municipalities in the region, 23 (55%) presented at least one rabies-positive case, while 13 of them had cases in bats. three distinct cycles of rabies were identified in the northwestern region of the state of s?o paulo: the urban cycle characterized predominantly by canine rabies (1993 to 1997); and the aerial and rural cycles starting in 1998, with predominance of cases in bats in urban areas and in herbivores.
Resistance of genetically modified potatoes to Potato virus Y under field conditions
Dusi, André Nepomuceno;Oliveira, Cristiane Lopes de;Melo, Paulo Eduardo de;Torres, Antonio Carlos;
Pesquisa Agropecuária Brasileira , 2009, DOI: 10.1590/S0100-204X2009000900009
Abstract: the objective of this work was to evaluate the resistance of genetically modified clones of potato to potato virus y (pvy) under field conditions. genetically modified plants were compared with nontransformed plants of the same cultivar. the plots were flanked with potato plants infected with both pvyo and pvyn strains (spread lines), in order to provide the experimental area with the source of virus, which was naturally spread by the native aphid population. the experiment was weekly monitored by visual inspections and by das-elisa in the plants produced from the harvested tubers, in order to evaluate the resistance of transgenic plants throughout the plant growth cycle. by the end of the third year, no infection symptoms were observed in the 1p clone; clone 63p showed 1% of infection, in contrast to about 90% of nontransformed plants infected. the stable expression of resistance to pvy provided by the coat protein gene was obtained in genetically modified clones of potato plants cultivar achat under field conditions, during three consecutive years.
Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles
Ferreira, Enderson Petr?nio de Brito;Dusi, André Nepomuceno;Xavier, Gustavo Ribeiro;Rumjanek, Norma Gouvêa;
Pesquisa Agropecuária Brasileira , 2008, DOI: 10.1590/S0100-204X2008000500008
Abstract: the objective of this work was to determine the shifts on the pcr-dgge profiles of bacterial communities associated to the rhizosphere of potato cultivars, in order to generate baseline information for further studies of environmental risk assessment of genetically modified potato plants. a greenhouse experiment was carried out with five potato cultivars (achat, bintje, agata, monalisa and asterix), cultivated in pots containing soil from an integrated system for agroecological production. the experiment was conducted in a split plot randomized block design with five cultivars, three sampling periods and five replicates. rhizosphere samples were collected in three sampling dates during plant development. dna of rhizosphere microorganisms was extracted, amplified by pcr using bacterial universal primers, and analyzed through dgge. shifts on the rhizosphere bacterial communities associated to rhizosphere of different cultivars were related to both cultivar and plant age. differences among rhizosphere bacterial communities were clearest at the earliest plant age, tending to decrease in later stages. this variation was detected among bacterial communities of the five tested cultivars. the characterization of soil microbial communities can be part of plant breeding programs to be used on studies of environmental risk assessment of genetically modified potatoes.
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