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Search Results: 1 - 10 of 41370 matches for " Ana-Clara AM Pires "
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The transmission of Leishmania infantum chagasi by the bite of the Lutzomyia longipalpis to two different vertebrates
Nagila FC Secundino, Vanessa C de Freitas, Carolina C Monteiro, Ana-Clara AM Pires, Bruna A David, Paulo FP Pimenta
Parasites & Vectors , 2012, DOI: 10.1186/1756-3305-5-20
Abstract: This study presents the establishment of a transmission model of Leishmania infantum chagasi by the bite of Lutzomyia longipalpis, the vector of American visceral leishmaniasis. The parasites were successfully transmitted by infected sandfly bites to mice and hamsters, indicating that both animals are good experimental models. The L. infantum chagasi dose that was transmitted in each single bite ranged from 10 to 10, 000 parasites, but 75% of the sandflies transmitted less than 300 parasites.The strategy for initiating infection by sandfly bite of experimental animals facilitates future investigations into the complex and dynamic mechanisms of visceral leishmaniasis. It is important to elucidate the transmission mechanism of vector bites. This model represents a useful tool to study L. infantum chagasi infection transmitted by the vector.Despite the fact that Leishmania is transmitted exclusively by sandfly vectors, a reproducible animal model of Leishmania infection transmitted by sandfly bite was only described in the year 2000 [1].Early investigations showed that infected P. papatasi sandflies can release from 0 to over 1,000 L. major promastigotes through their proboscides by forced feeding [2]. However, a transmission model by bite with P. duboscqui infected with L. major found that the parasite numbers inoculated in the host skin by one insect vector alone could vary from 10 to 100,000 [3].Considering the New World species of Leishmania, using a feeding device of chick skin membrane over culture medium showed that L. longipalpis sandflies infected with Leishmania mexicana (an unnatural vector/parasite pair) expelled an average of 1,000 parasites per fly [4]. Thus, even though L. longipalpis is the vector of L. infantum chagasi in nature, it is permissive in the laboratory to infection with other Leishmania species.In consideration of the continuing prevalence of American visceral leishmaniasis and the paucity of related studies in the literature, our goal was
K10-montmorillonite as support for a cationic manganese(III)-salen complex
Ku?niarska-Biernacka, Iwona;Pereira, Clara;Carvalho, Ana P.;Pires, Jo?o;Freire, Cristina;
Journal of the Brazilian Chemical Society , 2009, DOI: 10.1590/S0103-50532009000700017
Abstract: a commercial k10-montmorillonite was used as support for the immobilization of a cationic manganese(iii)-salen complex. the catalytic behavior of the resulting material was tested in the enantioselective epoxidation of three different alkenes, viz.: styrene, α-methylstyrene and 6-cyano-2,2-dimethylchromene, at 0 oc, using three different oxidant systems: m-chloroperoxybenzoic acid (m-cpba) and n-methylmorpholine (nmo) as co-oxidant, iodosylbenzene (phio) and sodium hypochlorite. all samples, before and after the catalytic studies, were characterised by nitrogen adsorption at -196 oc, drx, ftir and chemical analysis, in order to evaluate the influence of the substrate/oxidant system on catalyst integrity. the catalytic results demonstrated a strong influence of the substrate and oxidant system on the enantiomeric excess (%ee) values. in some cases, these values were comparable to those in the corresponding homogeneous phase reactions. the results also highlighted the importance of optimizing the oxidant systems, since they may lead to the disruption of the complex from the support, and the consequent catalyst deactivation.
Morfologia do desenvolvimento pós-seminal de Syngonanthus elegans e S. niveus (Eriocaulaceae): Eriocaulaceae
Scatena, Vera Lúcia;Lemos Filho, José Pires de;Lima, Ana Amélia Araújo;
Acta Botanica Brasilica , 1996, DOI: 10.1590/S0102-33061996000100008
Abstract: the species s. elegans (bong.) ruhl. and s. niveus (kunth.) ruhl. (eriocaulaceae) are known in brazil as star flowers and they grow in the rupestrian fields of serra do cipó - mg. they are used for ornamental purposes so data on their germination and postseminal development are important. in this study the seeds were germinated in growth chamber under controled conditions and in the laboratory under light and dark conditions. for each treatment four repetitions of 25 seeds on petri plates with humid filter paper were used. the results show that the seeds of s. elegans and s. niveus are positive photoblastic. the post-seminal development stages are the same for both species and during the germination period the undifferentiated axis of the embryo becomes apparent. the observation of the development of this axis shows that the growth of the leaves occurs first than that of the adventitious root. the seed operculum adheres to the testa and the primary root degenerates in the embrionary axis.
Bioavailability of immediate and controlled release formulations of lithium carbonate
Vismari, Luciana;Pires, Maria Laura N;Benedito-Silva, Ana Amélia;Calil, Helena Maria;
Revista Brasileira de Psiquiatria , 2002, DOI: 10.1590/S1516-44462002000200007
Abstract: introduction/objectives: controlled-release lithium formulations were developed to minimize elevated blood peaks, related to side-effects and intoxications. however, there is little information about the bioavailability of the only controlled-release lithium formulation available in brazil. the objective of this study was to compare the bioavailability of controlled-release and immediate-release lithium formulations, after single and multiple doses. methods: twelve healthy volunteers received 900 mg of immediate-release or controlled-release lithium carbonate in single or multiple doses during 9 days. after single dose administration, the following parameters were analyzed for each formulation: maximum lithium concentration (cmax); time to reach cmax (tmax); area under the curve of serum concentration versus time (auc0-12 and auc0-¥) and the elimination half-life (t1/2 elim.). after multiple doses, cmax; tmax; auc0-12; mean (cmean) and minimum drug concentration (cmin) and degree of fluctuation (df) were analyzed. a 90% confidence interval (90%ci) for the ratio between the aucs for each formulation was constructed. results/discussion: following single dose, the two formulations were bioequivalent; however, they were not after multiple doses. this fact could be a consequence of methodological limitations of lithium level's measurements since, following single dose, these levels could not be detected at time periods 24 and 48h in many volunteers, compromising the calculation of t1/2 elim ,and consequently of the auc0-¥ and the 90%ci to the ratio of these areas. therefore, the bioequivalence found after single dose may be an unreliable result.
Bioavailability of immediate and controlled release formulations of lithium carbonate
Vismari Luciana,Pires Maria Laura N,Benedito-Silva Ana Amélia,Calil Helena Maria
Revista Brasileira de Psiquiatria , 2002,
Abstract: INTRODUCTION/OBJECTIVES: Controlled-release lithium formulations were developed to minimize elevated blood peaks, related to side-effects and intoxications. However, there is little information about the bioavailability of the only controlled-release lithium formulation available in Brazil. The objective of this study was to compare the bioavailability of controlled-release and immediate-release lithium formulations, after single and multiple doses. METHODS: Twelve healthy volunteers received 900 mg of immediate-release or controlled-release lithium carbonate in single or multiple doses during 9 days. After single dose administration, the following parameters were analyzed for each formulation: maximum lithium concentration (Cmax); time to reach Cmax (t max); area under the curve of serum concentration versus time (AUC0-12 and AUC0-¥) and the elimination half-life (t1/2 elim.). After multiple doses, Cmax; t max; AUC0-12; mean (Cmean) and minimum drug concentration (Cmin) and degree of fluctuation (DF) were analyzed. A 90% confidence interval (90%CI) for the ratio between the AUCs for each formulation was constructed. RESULTS/DISCUSSION: Following single dose, the two formulations were bioequivalent; however, they were not after multiple doses. This fact could be a consequence of methodological limitations of lithium level's measurements since, following single dose, these levels could not be detected at time periods 24 and 48h in many volunteers, compromising the calculation of t1/2 elim ,and consequently of the AUC0-¥ and the 90%CI to the ratio of these areas. Therefore, the bioequivalence found after single dose may be an unreliable result.
Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
Baptista, Marcia L;Silva, Messias;Lima, Maria Amélia de;Yoshida, Clara FT;Gaspar, Ana Maria C;Galler, Ricardo;
Memórias do Instituto Oswaldo Cruz , 2006, DOI: 10.1590/S0074-02762006000700009
Abstract: the hepatitis a virus (hav) haf-203 strain was isolated from an acute case of hav infection. the primary isolation of haf-203 in brazil and its adaptation to the frhk-4 cell lineage allowed the production of large amounts of viral particles enabling molecular characterization of the first hav isolate in brazil. the aim of our study was to determine the nucleotide sequence of the haf-203 strain genome, compare it to other hav genomes and highlight its genetic variability. the complete nucleotide sequence of the haf-203 strain (7472 nucleotides) was compared to those obtained earlier by others for other hav isolates. these analyses revealed 19 haf-specific nucleotide sequence differences with 10 amino acid substitutions. most of the non-conservative changes were located at vp1, 2c, and 3d genes, but the 3b region was the most variable. the availability of haf-203 complementary dna was useful for the production of the recombinant vp1 protein, which is a major determinant of viral infectivity. this recombinant protein was shown by enzyme-linked immunoassay and blotting, to be immunogenic and resemble the native protein, therefore suggesting its value as a reagent for incorporation into diagnostic tests.
Desenvolvimento preliminar de gel de lapachol: estudo de permea??o in vitro
Lira, Ana Amélia Moreira;Sester, Elizangela de Araújo;Abreu, Luiz Renato Pires;Silva, Leila Bastos Leal da;Wanderley, Almir Gon?alves;Santana, Davi Pereira de;
Revista Brasileira de Ciências Farmacêuticas , 2004, DOI: 10.1590/S1516-93322004000100007
Abstract: the scope of this work was to develop two gel formulations containing lapachol, substance with known anti-inflammatory activity. in order to achieve a more effective topical delivery, the effects of vehicles and enhancers were evaluated. the influence of the carbopol? resin (934p, 940 and 941), the ph of the formulation (5, 7 and 8), the concentration of the active (0.2%, 0.5% and 1.0%) and the incorporation of tween? 80 were investigated. a series in vitro permeation studies were conduct in classical franz-type diffusion cells using durapore? synthetic membranes and wistar mouse skins as barriers. through the careful analyse of the data obtained, we could observe that the addition of tween? 80 leads to a significant increase in the in vitro diffusion of the active, except for the formulations containing carbopol? 934p and 0.2% of lapachol. the formulations assayed presented best in vitro diffusion rates of lapachol at ph 8 when 0.5% or 1.0% of the active was added. however, the preparations containing 1.0% of drug presented little insoluble crystals of lapachol.
BCG and BCG/DNAhsp65 Vaccinations Promote Protective Effects without Deleterious Consequences for Experimental Autoimmune Encephalomyelitis
Sofia Fernanda Gon?alves Zorzella-Pezavento,Clara Pires Fujiara Guerino,Fernanda Chiuso-Minicucci,Thais Graziela Donegá Fran?a,Larissa Lumi Watanabe Ishikawa,Ana Paula Masson,Célio Lopes Silva,Alexandrina Sartori
Journal of Immunology Research , 2013, DOI: 10.1155/2013/721383
Abstract: A prime-boost strategy conserving BCG is considered the most promising vaccine to control tuberculosis. A boost with a DNA vaccine containing the mycobacterial gene of a heat shock protein (pVAXhsp65) after BCG priming protected mice against experimental tuberculosis. However, anti-hsp65 immunity could worsen an autoimmune disease due to molecular mimicry. In this investigation, we evaluated the effect of a previous BCG or BCG/pVAXhsp65 immunization on experimental autoimmune encephalomyelitis (EAE) development. Female Lewis rats were immunized with BCG or BCG followed by pVAXhsp65 boosters. The animals underwent EAE induction and were daily evaluated for weight loss and clinical score. They were euthanized during recovery phase to assess immune response and inflammatory infiltration at the central nervous system. Previous immunization did not aggravate or accelerate clinical score or weight loss. In addition, this procedure clearly decreased inflammation in the brain. BCG immunization modulated the host immune response by triggering a significant reduction in IL-10 and IFN-γ levels induced by myelin basic protein. These data indicated that vaccination protocols with BCG or BCG followed by boosters with pVAXhsp65 did not trigger a deleterious effect on EAE evolution. 1. Introduction Tuberculosis (TB) is an infection caused by Mycobacterium tuberculosis and this disease remains one of the most important causes of death worldwide [1, 2]. Factors as coinfection with human immunodeficiency virus and emergence of drug resistance in M. tuberculosis strains have hampered TB control [3, 4]. The only available vaccine against TB is the attenuated M. bovis Bacillus Calmette-Guérin (BCG) that is recommended by the World Health Organization for all infants under 1 year of age. Around 100 million newborn children receive this vaccine and the global vaccine coverage is estimated to be 80% [5, 6]. In spite of this extensive use, numerous well-documented trials showed significant variation, from 0 to 80%, in BCG protective efficacy [7]. This has been attributed to variability in BCG vaccine strains and environmental factors as well as host genetic background [8, 9]. Although BCG seems to provide protection against disseminated tuberculosis in newborns and children, the induced immunity wanes with age, resulting in insufficient protection against adult pulmonary TB [10, 11]. In this context, there is a great interest in the development of new vaccines against TB. Numerous alternative living and nonliving putative TB vaccines are being lately tested [12–14]. Experimental
A natureza do poder: técnica e a??o social
Ribeiro, Ana Clara Torres;
Interface - Comunica??o, Saúde, Educa??o , 2000, DOI: 10.1590/S1414-32832000000200002
Abstract: this text questions the multiple-meaning character of the network notion, attempting to distinguish the current uses of this notion from those historically formulated by the social sciences. based on this indication, it deals with the network notion by means of two analytical alternatives: the reflection of power and the nature of the social relations encouraged by technical networks. the analysis is carried out largely through a reflection on the sense of social action, with due note being taken of the predominance of instrumental action over radical modernity.
Biotecnologia e desenvolvimento sustentável
Schenberg, Ana Clara Guerrini;
Estudos Avan?ados , 2010, DOI: 10.1590/S0103-40142010000300002
Abstract: biotechnology can play an important role to reach the goals of sustainability. in the present work, we describe successful examples of microorganisms especially designed for optimizing ethanol production, biodegradable plastics production from renewable resources, and toxic metals bioremediation. these biotechnological processes significantly contribute to promote sustainable development, although they may, at present, not be competitive with the conventional technologies.
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