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Search Results: 1 - 10 of 58527 matches for " Ana-Belen Martin-Cuadrado "
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Reconstructing Viral Genomes from the Environment Using Fosmid Clones: The Case of Haloviruses
Inmaculada Garcia-Heredia,Ana-Belen Martin-Cuadrado,Francisco J. M. Mojica,Fernando Santos,Alex Mira,Josefa Antón,Francisco Rodriguez-Valera
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0033802
Abstract: Metaviriomes, the viral genomes present in an environment, have been studied by direct sequencing of the viral DNA or by cloning in small insert libraries. The short reads generated by both approaches make it very difficult to assemble and annotate such flexible genomic entities. Many environmental viruses belong to unknown groups or prey on uncultured and little known cellular lineages, and hence might not be present in databases.
Metagenomic islands of hyperhalophiles: the case of Salinibacter ruber
Lejla Pa?i?, Beltran Rodriguez-Mueller, Ana-Belen Martin-Cuadrado, Alex Mira, Forest Rohwer, Francisco Rodriguez-Valera
BMC Genomics , 2009, DOI: 10.1186/1471-2164-10-570
Abstract: Three regions of the sequenced isolate were scarcely represented in the metagenome thus appearing to vary among co-occurring S. ruber cells. These metagenomic islands showed evidence of extensive genomic corruption with atypically low GC content, low coding density, high numbers of pseudogenes and short hypothetical proteins. A detailed analysis of island gene content showed that the genes in metagenomic island 1 code for cell surface polysaccharides. The strain-specific genes of metagenomic island 2 were found to be involved in biosynthesis of cell wall polysaccharide components. Finally, metagenomic island 3 was rich in DNA related enzymes.The genomic organisation of S. ruber variable genomic regions showed a number of convergences with genomic islands of marine microbes studied, being largely involved in variable cell surface traits. This variation at the level of cell envelopes in an environment devoid of grazing pressure probably reflects a global strategy of bacteria to escape phage predation.Prokaryotic genomes are extraordinarily plastic entities and vary widely within the limits of a well defined species. In order to describe these large genetic reservoirs the pan-genome concept was introduced [1]. According to this concept, the species genome is composed of a core genome, containing genes present in all (or most) strains and a variable genome, containing genes present only in some strains.In some cases, this variation is concentrated in hypervariable sets of genes, known as genomic islands [2-4]. Genomic island genes are often involved in specific lifestyles [5,6], e.g. symbiosis or pathogenesis [7,8] and frequently have the hallmarks of horizontally transferred genetic material such as different GC content or codon usage [9,10]. However, very little is known about the dynamic processes that originate and maintain the large genomic variability found in closely related prokaryotic genomes.Metagenomics provides a new way to look at the dynamics and flexibili
Metagenomics of the Deep Mediterranean, a Warm Bathypelagic Habitat
Ana-Belen Martín-Cuadrado, Purificación López-García, Juan-Carlos Alba, David Moreira, Luis Monticelli, Axel Strittmatter, Gerhard Gottschalk, Francisco Rodríguez-Valera
PLOS ONE , 2007, DOI: 10.1371/journal.pone.0000914
Abstract: Background Metagenomics is emerging as a powerful method to study the function and physiology of the unexplored microbial biosphere, and is causing us to re-evaluate basic precepts of microbial ecology and evolution. Most marine metagenomic analyses have been nearly exclusively devoted to photic waters. Methodology/Principal Findings We constructed a metagenomic fosmid library from 3,000 m-deep Mediterranean plankton, which is much warmer (~14°C) than waters of similar depth in open oceans (~2°C). We analyzed the library both by phylogenetic screening based on 16S rRNA gene amplification from clone pools and by sequencing both insert extremities of ca. 5,000 fosmids. Genome recruitment strategies showed that the majority of high scoring pairs corresponded to genomes from Rhizobiales within the Alphaproteobacteria, Cenarchaeum symbiosum, Planctomycetes, Acidobacteria, Chloroflexi and Gammaproteobacteria. We have found a community structure similar to that found in the aphotic zone of the Pacific. However, the similarities were significantly higher to the mesopelagic (500–700 m deep) in the Pacific than to the single 4000 m deep sample studied at this location. Metabolic genes were mostly related to catabolism, transport and degradation of complex organic molecules, in agreement with a prevalent heterotrophic lifestyle for deep-sea microbes. However, we observed a high percentage of genes encoding dehydrogenases and, among them, cox genes, suggesting that aerobic carbon monoxide oxidation may be important in the deep ocean as an additional energy source. Conclusions/Significance The comparison of metagenomic libraries from the deep Mediterranean and the Pacific ALOHA water column showed that bathypelagic Mediterranean communities resemble more mesopelagic communities in the Pacific, and suggests that, in the absence of light, temperature is a major stratifying factor in the oceanic water column, overriding pressure at least over 4000 m deep. Several chemolithotrophic metabolic pathways could supplement organic matter degradation in this most depleted habitat.
Fatores pré-operatórios associados à injúria renal aguda após cirurgia cardíaca: estudo prospectivo
Kochi, Ana Cláudia;Martins, Antonio Sergio;Lima, Maria Cristina Pereira;Martin, Luis Cuadrado;Balbi, André Luis;
Revista da Associa??o Médica Brasileira , 2008, DOI: 10.1590/S0104-42302008000300012
Abstract: objectives: to evaluate the clinical characteristics and co-morbidities associated with development of acute kidney injury (aki) after cardiac surgery in patients of a predominantly tertiary care university hospital. methods: one hundred and fifty cardiac surgery patients were prospectively studied at the botucatu school of medicine university hospital - unesp, using a protocol which started 48h before and finished 48h after surgery. aki was defined as a 30% increase in baseline serum creatinine. results: were expressed as mean ± standard deviation or median, with a significance level of 5%. results: there was a slight male predominance (57%); mean age of the studied population was 56±14 years. sixty-five per cent of patients underwent myocardial revascularization. aki was diagnosed in 34% of cases. multivariate analysis showed that age over 60 years and peripheral vascular disease were significantly associated with aki development. conclusion: aki was a common complication in cardiac surgery patients at this hospital. aki was associated with age over 60 years and presence of peripheral vascular disease.
PALINOLOGíA DE LOS GéNEROS MODIOLA, MODIOLASTRUM Y TROPIDOCOCCUS (MALVACEAE) Palynology of the genera Modiola, Modiolastrum and Tropidococcus (Malvaceae)
Graciela Ana Cuadrado
Bonplandia , 2010,
Abstract: Fueron estudiados y descriptos los granos de polen de las especies de tres géneros de Malvaceae, Modiola: M. caroliniana (L.) G. Don, Modiolastrum: M. australe Krapov., M. gilliesii (Steud.) Krapov., M. lateritium (Hook.) Krapov., M. malviflorum (Griseb.) K. Schum., M. palustre (Ekman) Krapov. y Tropidococcus: T. pinnatipartitus (A. St.- Hil. & Naudin) Krapov. A nivel genérico se identifican con facilidad e incluso es posible hacerlo a nivel específico sólo que en algunos casos, esto se consigue con caracteres de menor importancia filogenética. Los granos de polen son suboblatos, oblato esferoidales y esferoidales, de medianos a grandes. La ornamentación no muestra gran variabilidad y las siete especies son equinadas con nanoverrugas o espínulas entre ellas. Las aperturas son variadas en número (de tres a doce) y posición zonocolp(oid)oradas, espiraloide y dizonoaperturadas. Las características diferentes del polen de Tropidococcus pinnatipartitus (grandes, esferoidales, dizonoaperturados, número de aperturas doce), sustentan su separación del género Modiolastrum. Se presenta una clave para identificar las especies. El número básico de cromosomas de los tres géneros fue comparado con el número de aperturas de los granos de polen, resultando tener menos, los que tenían número básico de cromosomas más bajo, que los que lo tenían más alto. Se reconocieron cinco tipos de granos de polen teniendo en cuenta el número y disposición de las aperturas: 1) Tipo trizonocolporado: Modiolastrum malvifolium y M. palustre. 2) Tipo pentazonocolp(oid)orado: Modiolastrum australe y M. gilliesii. 3) Tipo pentacolp(oid)orado en disposición espiraloide: M. latehtium. 4) Tipo hexacolp(oid)orado en disposición espiraloide: Modiola caroliniana y 5) Tipo dizonocolp(oid)orado, ordenado en dos series de seis aperturas a cada lado del ecuador: Tropidococcus pinnatipartitus Pollen grains of seven species of three genera of Malvaceae are studied and described under optical and scanning microscopy: Modiola: M. caroliniana (L.) G. Don, Modiolastrum: M. australe Krapov., M. gilliesii (Steud.) Krapov., M. lateritium (Hook.) Krapov., M. malviflorum (Griseb.) K. Schum., M. palustre (Ekman) Krapov. and Tropidococcus: T. pinnatipartitus (A. St.-Hil. & Naudin) Krapov. They show clear differences at generic level and it is also possible to distinguish species, which in some cases are based on less important phylogenetic pollen characters. Pollen grains are sub oblate, oblate spheroidal and spheroidal; medium to big sized. Surfaces do not show great variability; the seven species are echinate, w
ESTUDIO MORFOLóGICO DEL POLEN DE BORDASIA BICORNIS (MALVACEAE) Pollen morphological study in Bordasia bicornis (Malvaceae)
Graciela Ana Cuadrado
Bonplandia , 2010,
Abstract: Fueron estudiados y descriptos los granos de polen de Bordasia bicornis Krapov. con microcopio óptico y microscopio electrónico de barrido. éstos presentaron afinidad polínica con especies del género Sida L, por lo que se compararon con 39 especies de Argentina, Brasil, Colombia, México, Sud áfrica, India, Tailandia, Filipinas Texas y Florida, EE.UU. del citado género, usando información bibliográfica y de la propia autora. Se hallaron afinidades en la estructura de la exina y altura de las espinas y diferencias como el número de aperturas y poros sin engrosamiento Pollen grains of Bordasia bicornis Krapov. were studied and described with light and scanning electron microscopy. They showed palynological affinity with species of genus Sida L, therefore they were compared with 39 species of Argentina, Brasil, Colombia. Mexico, South Africa, India, Thailand, Philippines, Texas and Florida U.S.A. Bibliographic and personal data were used. Affinities in the structure of the exine and height of the spines as well as differences in apertures number and pores without thicker ring were found
Avalia??o do desempenho diagnóstico e do valor de corte para o índice de respira??o rápida e superficial na predi??o do insucesso da extuba??o
Danaga, Aline Roberta;Gut, Ana Lúcia;Antunes, Letícia Cláudia de Oliveira;Ferreira, Ana Lúcia dos Anjos;Yamaguti, Fábio Akio;Christovan, José Carlos;Teixeira, Ubirajara;Guedes, Cristina Aparecida Veloso;Sasseron, Ana Beatriz;Martin, Luis Cuadrado;
Jornal Brasileiro de Pneumologia , 2009, DOI: 10.1590/S1806-37132009000600007
Abstract: objective: to evaluate the diagnostic performance of the rapid shallow breathing index (rsbi) in predicting extubation failure among adult patients in the intensive care unit and to determine the appropriateness of the classical rsbi cut-off value. methods: this was a prospective study conducted in the adult intensive care unit of the botucatu school of medicine hospital das clínicas. the rsbi was evaluated in 73 consecutive patients considered clinically ready for extubation. results: the classical rsbi cut-off value (105 breaths/min/l) presented a sensitivity of 20% and a specificity of 95% (sum = 115%). analysis of the receiver operator characteristic (roc) curve revealed a better cut-off value (76.5 breaths/min/l), which presented a sensitivity of 66% and a specificity of 74% (sum = 140%). the area under the roc curve for the rsbi was 0.78. conclusions: the classical rsbi cut-off value proved inappropriate, predicting only 20% of the cases of extubation failure in our sample. the new cut-off value provided substantial improvement in sensitivity, with an acceptable loss of specificity. the area under the roc curve indicated that the discriminative power of the rsbi is satisfactory, which justifies the validation of this index for use.
Steviol Glycoside Content Dynamics during the Growth Cycle of Stevia rebaudiana Bert  [PDF]
Ana Belen Guerrero, Leticia San Emeterio, Itziar Dome?o, Ignacio Irigoyen, Julio Muro
American Journal of Plant Sciences (AJPS) , 2018, DOI: 10.4236/ajps.2018.94068
Abstract: The sweetener compounds in Stevia, steviol glycosides (SG), are mainly found in the leaves. The SG content depends on the plant’s growth stage and is usually highest just before flowering. In temperate areas, Stevia is a polyannual crop (5 - 7 years) with a vegetative period lasting from April-May until October, during which time the crop can be harvested two or three times. This research focuses on the need for knowledge about Stevia’s response to temperate climates in Southern Europe. Two field assays were established from June to October 2013 at two sites in Navarra (Spain). The biomass and content of the two major SG, stevioside (ST) and rebaudioside A (RA), were measured using High Performance Liquid Chromatography (HPLC) in 66 cloned plants, at different developmental stages. Although the results from the two sites showed different SG leaf content dynamics during the plant growth, the optimum harvest date at both sites coincided with the bud-flowering stage at the beginning of September (around 96 days after planting), when a ST yield of 27 g·m-2 was reached. These results show Stevia’s potential as a commercial crop for stevioside production in northern Spain.
Tratamento da doen?a arterial coronariana em renais cr?nicos em diálise do Hospital das Clínicas da Faculdade de Medicina de Botucatu - UNESP
Vieira, Paula Ferreiro;Garcia, Paula Dalsoglio;Bregagnollo, Edson Antonio;Carvalho, Fábio Cardoso;Kochi, Ana Cláudia;Martins, Antonio Sérgio;Caramori, Jaqueline Costa Teixeira;Franco, Roberto Jorge da Silva;Barretti, Pasqual;Martin, Luis Cuadrado;
Arquivos Brasileiros de Cardiologia , 2007, DOI: 10.1590/S0066-782X2007000500005
Abstract: background: interventional treatment of coronary insufficiency is underemployed among dialysis patients. studies confirming its efficacy in this set of patients are scarce. objective: to assess the results of interventional treatment of coronary artery disease in patients undergoing dialysis. methods: a total of 34 dialysis patients submitted to coronary angiography between september 1995 and october 2004 were divided according to presence or absence of coronary lesion, type of treatment and presence or absence of diabetes mellitus. the groups were compared according to their clinical and survival characteristics. survival of patients undergoing interventional treatment was compared to overall survival of 146 dialysis patients at the institution in the same period. interventional treatment was indicated to the same clinical conditions in the general population. results: thirteen patients with no angiography coronary lesions presented a survival rate of 100% in 48 months as compared to 35% of 21 patients with coronary artery disease. diabetic patients had a lower survival rate compared with non-diabetics. angioplasty had a worse prognosis compared to surgery; however, 80% of patients undergoing angioplasty were diabetic. seventeen patients submitted to interventional procedures presented a survival rate similar to that of the others 146 hemodialysis patients without clinical evidence of coronary disease. conclusion: this small series shows that myocardial revascularization, whenever indicated, can be performed in dialysis patients. this conclusion is corroborated by similar mortality rates in two groups of patients: coronary patients submitted to revascularization and overall dialysis patients.
Cellular Prion Protein and Sexual Dimorphic Areas in Rodents. Correlates with Alzheimer Disease  [PDF]
Mar Cuadrado-Tejedor, Ana Irujo, Beatriz Paternain, María Jesús Madrid, José Luis Velayos
Neuroscience & Medicine (NM) , 2011, DOI: 10.4236/nm.2011.24051
Abstract: The cellular prion protein (PrPC) expression was analyzed by western-blot in the rat, in two different dimorphic brain areas such as the anterior hypothalamic and the preoptic areas. In both cases, the PrPC expression was increased in males, implying a sexual dimorphism for the PrPC protein. The study was also made in other two brain areas, frontal cortex and hyppocampus (a clearly dimorphic area); in this case, mice of different ages of both sexes were used. In both brain areas analyzed, although the PrPC expression was increased with age until the adult age (38 weeks), it was decreased in aged animals (56 weeks) in both sexes. The PrPC expression in mouse hippocampus was predominant in males in comparison to females. Moreover, the non-glycosylated band was increased with age and this increase was parallel with the increase observed for the glycolsylated band. The non-glycosylated band increases more in aged females. Altogether, these data suggest that PrP in rodents, in the brain areas analyzed, has a dimorphism role. As we discuss in the present study and in relation to previous studies of our group these data could be extrapolated to humans (specially in Alzhemer disease cases).
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