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Search Results: 1 - 10 of 252413 matches for " Ana Karine C. Monteiro "
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Effect of an Educational Intervention by Attendance and at Distance on Nurses’ Knowledge about Pressure Ulcer  [PDF]
Elaine M. L. R. Andrade, Patrícia A. L. Cavalcanti, Ana Karoline C. Monteiro, Ana Karine C. Monteiro, Maria Helena B. A. Luz, José M. M. Neto, Isabel A. C. Mendes
Creative Education (CE) , 2014, DOI: 10.4236/ce.2014.518185
Abstract: The effect of an educational intervention by attendance and at distance on the knowledge of nurses about pressure ulcer (PU) was verified. An experimental research with a post-test control group was undertaken. A randomized sample of 43 nurses in a Control Group (CG) (N = 20) was submitted to an educational intervention by attendance and an Experimental Group (EG) (N = 23) was submitted to an intervention at distance. Data were collected between January and April 2012 in three phases, which were: location, invitation, registration and adaptation of the nurses to Moodle, educative intervention and post-test. The mean number of correct answers on the posttest among the nurses who participated in the intervention by attendance was 34.0 (sd = 3.3), against 36.2 (sd = 2.7) for the nurses in the distance education intervention. This difference in means was statistically significant (p = 0.019). The research evidenced that continuing education in nursing through distance education (DE) can be effective due to its practicality and interactivity and because it allows nurses in clinical practice to decide on the best time and place to access the content and improve their knowledge about PU.
Plasmodium falciparum Infection Induces Expression of a Mosquito Salivary Protein (Agaphelin) That Targets Neutrophil Function and Inhibits Thrombosis without Impairing Hemostasis
Michael Waisberg ,Alvaro Molina-Cruz,Daniella M. Mizurini,Nidhi Gera,Beatriz C. Sousa,Dongying Ma,Ana C. Leal,Tainá Gomes,Michalis Kotsyfakis,José M. C. Ribeiro,Jan Lukszo,Karine Reiter,Stephen F. Porcella,Carlo J. Oliveira,Robson Q. Monteiro,Carolina Barillas-Mury,Susan K. Pierce,Ivo M. B. Francischetti
PLOS Pathogens , 2014, DOI: doi/10.1371/journal.ppat.1004338
Abstract: Background Invasion of mosquito salivary glands (SGs) by Plasmodium falciparum sporozoites is an essential step in the malaria life cycle. How infection modulates gene expression, and affects hematophagy remains unclear. Principal Findings Using Affimetrix chip microarray, we found that at least 43 genes are differentially expressed in the glands of Plasmodium falciparum-infected Anopheles gambiae mosquitoes. Among the upregulated genes, one codes for Agaphelin, a 58-amino acid protein containing a single Kazal domain with a Leu in the P1 position. Agaphelin displays high homology to orthologs present in Aedes sp and Culex sp salivary glands, indicating an evolutionarily expanded family. Kinetics and surface plasmon resonance experiments determined that chemically synthesized Agaphelin behaves as a slow and tight inhibitor of neutrophil elastase (KD~10 nM), but does not affect other enzymes, nor promotes vasodilation, or exhibit antimicrobial activity. TAXIscan chamber assay revealed that Agaphelin inhibits neutrophil chemotaxis toward fMLP, affecting several parameter associated with cell migration. In addition, Agaphelin reduces paw edema formation and accumulation of tissue myeloperoxidase triggered by injection of carrageenan in mice. Agaphelin also blocks elastase/cathepsin-mediated platelet aggregation, abrogates elastase-mediated cleavage of tissue factor pathway inhibitor, and attenuates neutrophil-induced coagulation. Notably, Agaphelin inhibits neutrophil extracellular traps (NETs) formation and prevents FeCl3-induced arterial thrombosis, without impairing hemostasis. Conclusions Blockade of neutrophil elastase emerges as a novel antihemostatic mechanism in hematophagy; it also supports the notion that neutrophils and the innate immune response are targets for antithrombotic therapy. In addition, Agaphelin is the first antihemostatic whose expression is induced by Plasmodium sp infection. These results suggest that an important interplay takes place in parasite-vector-host interactions.
Entre o estranho e o familiar: o uso de analogias no ensino de história
Monteiro, Ana Maria F. C;
Cadernos CEDES , 2005, DOI: 10.1590/S0101-32622005000300006
Abstract: based on the contributions of authors who use the concept of "school knowledge" and consider it as an original and specific creation of the school culture, this paper discusses the use of analogies in history teaching. since they help establish symbolic mediations and meaningful learning, analogies are often used by history teachers as a tool to help students understand school contents. they are recognized as a helpful resource to surpass the strangeness of unknown facts which become familiar by means of the comparisons provided. by relating scientific information to common sense, they help to transform the meanings of concepts and practices which are being studied, and configure an emblematic creation of school knowledge. nevertheless, the risk of anachronism requires teachers be attentive and cautious to avoid errors
Association of the infusion of Heteropterys aphrodisiaca and endurance training brings spermatogenetic advantages
Gomes,Marcos L M; Monteiro,Juliana C; Freitas,Karine M; Sbervelheri,Mariana M; Dolder,Heidi;
Biological Research , 2011, DOI: 10.4067/S0716-97602011000300004
Abstract: the species heteropterys aphrodisiaca is commonly used as a stimulant by popular medicine in the cerrado, a savanna-like biome, brazil. recent studies have proved its protective effects on testes of animals submitted to treatment using cyclosporine a, as well as its stimulus effect in increasing testosterone secretion. therefore, the present study was designed to analyze whether the association of the plant infusion and endurance exercise could potentiate the stimulating effect. the animals were separated into 4 groups: two control (sedentary and trained) receiving water and two treated (sedentary and trained) receiving the plant infusion daily (104mg/day). the proportion of the seminiferous tubule compartment and interstitium was analyzed. within the seminiferous epithelium, the number of sertoli and germ cells were counted in order to evaluate whether the treatment would alter the spermatogenic dynamics, analyzing: the spermatogenic yield, the mitotic and meiotic indexes, the total number of germ cells and the sertoli cell support capacity. trained and treated animals showed increased spermatogenic yield and spermatogonia mitosis, and no significant differences in apoptotic indexes. despite the results showing the same pattern regarding yield and mitotic index, the meiotic index was higher in the sedentary/treated group. therefore, the h. aphrodisiaca infusion increased both the testosterone production and the spermatogonia mitosis, thus increasing the spermatogenic yield.
Association of the infusion of Heteropterys aphrodisiaca and endurance training brings spermatogenetic advantages
Marcos L M Gomes,Juliana C Monteiro,Karine M Freitas,Mariana M Sbervelheri
Biological Research , 2011,
Abstract: The species Heteropterys aphrodisiaca is commonly used as a stimulant by popular medicine in the Cerrado, a savanna-like biome, Brazil. Recent studies have proved its protective effects on testes of animals submitted to treatment using Cyclosporine A, as well as its stimulus effect in increasing testosterone secretion. Therefore, the present study was designed to analyze whether the association of the plant infusion and endurance exercise could potentiate the stimulating effect. The animals were separated into 4 groups: two control (sedentary and trained) receiving water and two treated (sedentary and trained) receiving the plant infusion daily (104mg/day). The proportion of the seminiferous tubule compartment and interstitium was analyzed. Within the seminiferous epithelium, the number of Sertoli and germ cells were counted in order to evaluate whether the treatment would alter the spermatogenic dynamics, analyzing: the spermatogenic yield, the mitotic and meiotic indexes, the total number of germ cells and the Sertoli cell support capacity. Trained and treated animals showed increased spermatogenic yield and spermatogonia mitosis, and no significant differences in apoptotic indexes. Despite the results showing the same pattern regarding yield and mitotic index, the meiotic index was higher in the sedentary/treated group. Therefore, the H. aphrodisiaca infusion increased both the testosterone production and the spermatogonia mitosis, thus increasing the spermatogenic yield.
Immobilization of a Commercial Lipase from Penicillium camembertii (Lipase G) by Different Strategies
Adriano A. Mendes,Larissa Freitas,Ana Karine F. de Carvalho,Pedro C. de Oliveira,Heizir F. de Castro
Enzyme Research , 2011, DOI: 10.4061/2011/967239
Abstract: The objective of this work was to select the most suitable procedure to immobilize lipase from Penicillium camembertii (Lipase G). Different techniques and supports were evaluated, including physical adsorption on hydrophobic supports octyl-agarose, poly(hydroxybutyrate) and Amberlite resin XAD-4; ionic adsorption on the anionic exchange resin MANAE-agarose and covalent attachment on glyoxyl-agarose, MANAE-agarose cross-linked with glutaraldehyde, MANAE-agarose-glutaraldehyde, and epoxy-silica-polyvinyl alcohol composite. Among the tested protocols, the highest hydrolytic activity (128.2 ± 8.10?IU·g?1 of support) was achieved when the lipase was immobilized on epoxy-SiO2-PVA using hexane as coupling medium. Lipase immobilized by ionic adsorption on MANAE-agarose also gave satisfactory result, attaining 55.6 ± 2.60?IU·g?1 of support. In this procedure, the maximum loading of immobilized enzyme was 9.3?mg·g?1 of gel, and the highest activity (68.8 ± 2.70?IU·g?1 of support) was obtained when 20?mg of protein·g?1 was offered. Immobilization carried out in aqueous medium by physical adsorption on hydrophobic supports and covalent attachment on MANAE-agarose-glutaraldehyde and glyoxyl-agarose was shown to be unfeasible for Lipase G. Thermal stability tests revealed that the immobilized derivative on epoxy-SiO2-PVA composite using hexane as coupling medium had a slight higher thermal stability than the free lipase. 1. Introduction Lipases (triacylglycerol acyl hydrolases EC 3.1.1.3) are hydrolases that act on carboxylic ester bonds. The natural physiologic role of lipases is the hydrolysis of triglycerides into fatty acids and glycerol, but they can also catalyze esterifications and interesterifications in nonaqueous media [1–5]. A peculiarity mechanism action of lipases is the interfacial activation. Most lipases have a α-helical oligopeptide structure covering their active site (lid or flap) and making them inaccessible to substrates. In the absence of a hydrophobic interface, the active site is secluded from the reaction medium, and the enzyme is in the so-called “closed conformation.” However, in the presence of a hydrophobic interface (a drop of oil), the lipase changes its conformation and exposes the catalytic site to the hydrophobic phase, yielding the “open conformation” [6–9]. The limitations of the industrial use of lipases have been mainly due to their high cost, which may be overcome by immobilization techniques on solid supports. Immobilization facilitates the separation of products and provides more flexibility with enzyme/substrate contact by
Aplica??o do Processo de Enfermagem: estudo de caso com uma puérpera
Alves, Albertisa Rodrigues;Chaves, Edna Maria Camelo;Freitas, Maria Célia de;Monteiro, Ana Ruth Macedo;
Revista Brasileira de Enfermagem , 2007, DOI: 10.1590/S0034-71672007000300019
Abstract: the study aimed at implementing the nursing process for a post-partum woman with cardiomyopathy after cardiorespiratory arrest. we conducted a clinical case study, in an intensive care unit of fortaleza, ceará, brazil, in the period from september to october, 2005. the data were obtained after evaluation for elaboration of nursing diagnoses according to nanda taxonomy ii, clinical judgment, intervention and goals. the nursing diagnoses found were: impaired verbal communication, decreased cardiac output, inefficient respiratory pattern, excessive volume of fluids, diarrhea, impaired physical mobility and deficit in selfcare. we highlighted the importance of the process as focus of the nurse's work in the clinic, in order to favor the patient's return to his family context as well as give credibility to the nurse's job.
Enfermagem e a promo??o da saúde mental na família: uma reflex?o teóric
Macêdo, Virgílio César Dourado de;Monteiro, Ana Ruth Macêdo;
Texto & Contexto - Enfermagem , 2004, DOI: 10.1590/S0104-07072004000400011
Abstract: the family is considered indispensable for the guarantee of the survival and protection of its members, providing the affection and material support necessary for development and well-being, being in its interior absolved ethical values deepened bows, and solidarity. the family needs to be qualified to supply those needs, being indispensable the maintenance of its mental health. the article provokes reflection on family relationships, vital cycles and values in the family and on the family's nursing, searching to promote the discussion about possibilities to offer support in mental health in the families, furthering an improvement in the quality of interaction among its members.
Educa??o e saúde mental na família: experiência com grupos vivenciais
Macêdo, Virgílio César Dourado de;Monteiro, Ana Ruth Macêdo;
Texto & Contexto - Enfermagem , 2006, DOI: 10.1590/S0104-07072006000200005
Abstract: this is a descriptive study approaching children's families of fundamental teaching, in partnership with a public school of fundamental and grade school teaching in fortaleza, ce, brazil. it seeks to create living groups within these families; to identify difficulties lived in the family relationships; and to know the perception and the influence of the session groups in the family dynamics. there were eight meetings in the school between april and october of 2003, with good interaction and important exchange of experience among the parents. after the meetings, some participant families were interviewed, selecting them for areas of discussion about their children's education, intra-family relationships, and reception of parents` proposal by the group in the school as contribution for the maintenance of the balance in the intra family relationships. the accomplished study evidenced, through family living groups, possibilities to offer support in mental health, propitiating improvement in the quality of interaction among their members.
Educa o e saúde mental na família: experiência com grupos vivenciais
Virgílio César Dourado de Macêdo,Ana Ruth Macêdo Monteiro
Texto & Contexto - Enfermagem , 2006,
Abstract: Estudio de naturaleza descriptiva abordando a las familias de los ni os de la educación primaria con el apoyo de una escuela pública de educación primaria y secundaria en Fortaleza, CE. Se buscó: realizar grupos vivenciales con éstas familias; identificar las dificultades vivenciadas en las relaciones familiares y conocer la percepción e influencia de las sesiones grupales en la dinámica familiar. Se realizaron ocho encuentros en la escuela entre abril y octubre del 2003, con una buena interacción e importante intercambio de experiencias entre los padres. Después de los encuentros, se entrevistaron algunas familias participantes, se alándolas como un espacio de discusión sobre la educación de los hijos y las relaciones intrafamiliares y la acogida a la propuesta de un grupo de padres en la escuela como una contribución para el mantenimiento del equilibrio en las relaciones intrafamiliares. El estudio realizado evidenció, a través de los grupos vivenciales con familias, las posibilidades de ofrecer promoción en la salud mental, propiciando una mejoría en la calidad de la interación entre sus miembros.
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