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Search Results: 1 - 10 of 170285 matches for " Amanda E. Rossiter "
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E. coli Fis Protein Insulates the cbpA Gene from Uncontrolled Transcription
Kiran Chintakayala equal contributor,Shivani S. Singh equal contributor,Amanda E. Rossiter,Rajesh Shahapure,Remus T. Dame,David C. Grainger
PLOS Genetics , 2013, DOI: 10.1371/journal.pgen.1003152
Abstract: The Escherichia coli curved DNA binding protein A (CbpA) is a poorly characterised nucleoid associated factor and co-chaperone. It is expressed at high levels as cells enter stationary phase. Using genetics, biochemistry, and genomics, we have examined regulation of, and DNA binding by, CbpA. We show that Fis, the dominant growth-phase nucleoid protein, prevents CbpA expression in growing cells. Regulation by Fis involves an unusual “insulation” mechanism. Thus, Fis protects cbpA from the effects of a distal promoter, located in an adjacent gene. In stationary phase, when Fis levels are low, CbpA binds the E. coli chromosome with a preference for the intrinsically curved Ter macrodomain. Disruption of the cbpA gene prompts dramatic changes in DNA topology. Thus, our work identifies a novel role for Fis and incorporates CbpA into the growing network of factors that mediate bacterial chromosome structure.
Mutational and Topological Analysis of the Escherichia coli BamA Protein
Douglas F. Browning, Sophie A. Matthews, Amanda E. Rossiter, Yanina R. Sevastsyanovich, Mark Jeeves, Jessica L. Mason, Timothy J. Wells, Catherine A. Wardius, Timothy J. Knowles, Adam F. Cunningham, Vassiliy N. Bavro, Michael Overduin, Ian R. Henderson
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0084512
Abstract: The multi-protein β-barrel assembly machine (BAM) of Escherichia coli is responsible for the folding and insertion of β-barrel containing integral outer membrane proteins (OMPs) into the bacterial outer membrane. An essential component of this complex is the BamA protein, which binds unfolded β-barrel precursors via the five polypeptide transport-associated (POTRA) domains in its N-terminus. The C-terminus of BamA contains a β-barrel domain, which tethers BamA to the outer membrane and is also thought to be involved in OMP insertion. Here we mutagenize BamA using linker scanning mutagenesis and demonstrate that all five POTRA domains are essential for BamA protein function in our experimental system. Furthermore, we generate a homology based model of the BamA β-barrel and test our model using insertion mutagenesis, deletion analysis and immunofluorescence to identify β-strands, periplasmic turns and extracellular loops. We show that the surface-exposed loops of the BamA β-barrel are essential.
Report: Creative Labour and the Role of Intellectual Property
Ned Rossiter
Fibreculture Journal , 2003,
Abstract: This article reports on findings from a survey posted to the fibreculture mailing list prior to the 3rd annual fibreculture meeting in Brisbane this July. Broadly speaking, the survey was interested in the extent to which the exploitation of intellectual property defines the condition of labour within the creative industries. At a methodological level, the article challenges prevailing assumptions about conducting empirical research in new media studies and enlists a processual media theory approach as a technique for drawing out the relationships between the condition of creative labour and reflexive, non-linear media-information systems of communication. Central to this article is an argument about the merits of the Italian autonomist concept of 'immaterial labour' against what I term 'disorganised labour'. The article suggests that the latter more accurately describes the current condition of creative labour. The article concludes by advocating the political strategy of organising creative labour in the form of networks rather than the traditional model of the party, as adopted by various unions.
Synthesis and Chiral Separation of Fibratol, Isopropyl 2-(4-((4-chlorophenyl)(hydroxyl) methyl)-phenoxy)-2-methylpropanoate  [PDF]
Amanda E. Kotheimer, Wahajul Haq, Ganesaratnam K. Balendiran
International Journal of Organic Chemistry (IJOC) , 2018, DOI: 10.4236/ijoc.2018.82015
Abstract: Practical synthetic route for the formation of enantiomeric mixture of Isopropyl 2-(4-((4-chlorophenyl)(hydroxyl)methyl)phenoxy)-2-methylpropanoate (Fibratol 2a/b) from isopropyl 2-(4-(4-chlorobenzoyl)phenoxy)-2-methylpropanoate (Fenofibrate 1) has been developed. Method has also been established for the chiral separation of enantiomers of Fibratol 2a/b that is synthesized using the route mentioned above. The optical activity determined for enantiomerically separated Fibratol (2a) and Fibratol (2b) are -5.2° and 8.0° which reflect their ability to rotate plane polarized light counterclockwise (levo) and clockwise (dextro), respectively.
How to Use Qualitative Research to Design a Managerially Useful E-Service Questionnaire
Rossiter,John R;
Journal of theoretical and applied electronic commerce research , 2012, DOI: 10.4067/S0718-18762012000200006
Abstract: e-service questionnaires must be content-aligned with the company or organization's customer relationship management system (crm). four phases of qualitative research will ensure this alignment. the first phase is a qualitatively evaluative search of the practitioner literature on e-retailing, both b2b and b2c, and on crm so as to capture evolving knowledge in both fields. the second phase is individual depth interviews (idis) with potential, current, and lapsed customers to map their e-interactive behavior and experiences. the third phase is dyadic depth interviews (ddis) with the marketing manager and the website designer to fully understand the company's current and potential e-service and crm capabilities. the e-service questionnaire can then be designed according to the general guidelines presented in this article and using question-and-answer templates provided in the author's previous article [14]. the final phase of qualitative research will consist of post-survey idis with a sample of the original survey respondents to clarify and elaborate on the survey's findings, followed by a final manager-designer ddi to implement the findings.
Dawn of the Organised Networks
Geert Lovink,Ned Rossiter
Fibreculture Journal , 2005,
Abstract: This article introduces the political concept of "organised networks". We explore alternative currencies, critique blogs, wikis and Creative Commons. We revisit the legacy of techno-libertarians. We look at why scale is such a difficult thing for networks to negotiate - particularly tactical media networks, for our interests. Along the way we also have a running critique of liberal democracy. And we end up wondering if criminal networks might be angel investors of some kind of another.
From Precarity to Precariousness and Back Again: Labour, Life and Unstable Networks
Brett Neilson,Ned Rossiter
Fibreculture Journal , 2005,
Abstract: This article explores the distinction and passage between "precarity" and "precariousness". In surveying the various ways in which these terms have circulated, we wish to establish a framework within which questions of labour, life and social-political organisation can be understood. The various uncertainties defining contemporary life are carried over - and, we argue, internal to - the logic of informatisation. Our aim, however, is not to collapse respective differences into a totalising logic that provides a definitive assessment or system of analysis; rather, we seek to identify some of the forces, rhythms, discourses and actions that render notions such as creativity, innovation, and organisation, along with the operation of capital, with a complexity whose material effects are locally situated within transversal networks. Where there are instances of inter-connection between, say, the work of migrants packaging computer parts or cleaning offices and that of media labour in a call centre, software development firm or digital post-production for a film studio, we see a common expressive capacity predicated on the dual conditions of exploitation and uncertainty.
The Role of Local Authorities in Addressing Human Trafficking
Kathryn Rossiter,Jo Benfield
Commonwealth Journal of Local Governance , 2009, DOI: 10.5130/cjlg.v0i0.1098
Abstract:
Persistent Copulation in Asexual Female?? Potamopyrgus antipodarum: Evidence for Male Control with Size-Based Preferences
Amanda E. Nelson,Maurine Neiman
International Journal of Evolutionary Biology , 2011, DOI: 10.4061/2011/439046
Abstract: Transitions from sexual to asexual reproduction provide a useful context for investigating the evolutionary loss of nonfunctional traits. It is often assumed that useless behaviors or structures will degrade, but this process is poorly understood. Potamopyrgus antipodarum is an ancestrally sexual New Zealand freshwater snail characterized by numerous independent transitions to asexual all-female lineages. The availability of multiple independently-derived asexual lineages of various time since derivation from sexual ancestors means that the P. antipodarum system is well-suited for the study of trait loss related to mating behavior and copulation. Here, we asked whether mating behavior in asexual female P. antipodarum degrades with increasing asexual lineage age. While copulation frequency did not differ in females from old versus young asexual lineages, post hoc analyses indicated that it was instead positively associated with mean lineage female size. We observed that female P. antipodarum take a passive physical role in copulatory interactions, indicating that female behavior may not be a useful variable for detection of sex-related vestigialization in this system. Instead, males seem to be in proximate control of copulation frequencies, meaning that male mating behavior may be a primary determinant of the expression of mating behavior in asexual female P. antipodarum. 1. Introduction Vestigialization, the evolutionary loss of formerly useful and presently nonfunctional morphological, molecular, and behavioral traits, is an important evolutionary process that remains poorly understood [1–3]. The multiple independent transitions from sexual to asexual reproduction across eukaryotic phylogeny [4] and the likelihood that mating is costly [5–14] provide a powerful context in which to pose questions regarding rates of vestigialization. For example, we might expect that parthenogenetic lineages that do not need to copulate or be fertilized by another individual to reproduce may experience relaxed selection for the maintenance of sexual structures and behaviors, setting the stage for trait loss [1, 5, 15–17]. Selection may even actively favor the loss of traits related to mating behavior and copulation in asexual lineages if these traits are costly [1–3]. Empirical examples of decay of mating behavior associated with transitions to asexuality include the documentation of increasing reluctance to mate in female Drosophila mercatorum following several generations of artificial selection for parthenogenetic reproduction [18] and the observation of decay of
Real Quadratic Fields In Which Every Non-Maximal Order Has Relative Class Number Greater Than One
Amanda Furness,Adam E. Parker
Mathematics , 2012,
Abstract: Cohn asks if for every real quadratic field Q(m) with discriminant d there exists a non-maximal order corresponding to f > 1 such that the relative class number Hd(f) = h(f2d)/h(d) is one. We prove that when m = 46 (and in seven other cases) there is no such order.
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