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Search Results: 1 - 10 of 2312 matches for " Amalia Montes "
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Visceral Leishmaniosis caused by Leishmania (L.) mexicana in a Mexican patient with human immunodeficiency virus infection
Ramos-Santos, Carmen;Hernandez-Montes, Omar;Sanchez-Tejeda, Gustavo;Monroy-Ostria, Amalia;
Memórias do Instituto Oswaldo Cruz , 2000, DOI: 10.1590/S0074-02762000000500022
Abstract: a 36 year old male was admitted in december 1997 to hospital with afternoon fever, malaise and hepatosplenomegaly. he also had a dry cough, dyspnoea and anaemia. pneumonia caused by pneumocystis carinii and human immunodeficiency virus (hiv) infection were documented. the hiv infection was confirmed in 1997 with 290,000 virus copies. the patient had been in the mexican state of chiapas which is known to be endemic for visceral leishmaniosis (vl) and localized cutaneous leishmaniosis (lcl). the visceral symptoms were diagnosed as vl and the causal agent was identified as leishmania (l.) mexicana. identification of leishmania was carried out by the analysis of amplified dna with specific primers belonging to the leishmania subgenus and by dot blot positive hybridisation of these polymerase chain reaction derived products with kdna from the l. (l.) mexicana mc strain used as probe. this is the first case in mexico of vl caused by a species of leishmania that typically produces a cutaneous disease form.
Riverón,Yamilka; Montes de Oca,Nivian; Villoch,Alejandra; Vidal,Aniteé; Nú?ez,Amalia;
Revista de Salud Animal , 2010,
Abstract: the natural characteristics of biological agents by themselves allow their potential use for hostile purposes or in armed conflict and it would be impossible to foresee all possible dissemination methods of these entities. the biological weapons convention (bwc) is intended to prohibit the development, production, stockpiling and acquisition or transfer of biological and toxin weapons. the national center for agricultural health (censa), when enrolled in the registry reorganizes internal safeguarding culture collections activity to ensure compliance to resolution 2 / 2004 citma. with this objective, a system for inventory and retained control of biological material that demonstrates compliance with the regulations established in the country to ensure confidence in the handling of biological material, equipment and technologies and satisfy the requirements of the cab, while also ensuring the processes of research, production and technical-services which in this center are developed.
Visceral Leishmaniosis caused by Leishmania (L.) mexicana in a Mexican patient with human immunodeficiency virus infection
Ramos-Santos Carmen,Hernandez-Montes Omar,Sanchez-Tejeda Gustavo,Monroy-Ostria Amalia
Memórias do Instituto Oswaldo Cruz , 2000,
Abstract: A 36 year old male was admitted in December 1997 to hospital with afternoon fever, malaise and hepatosplenomegaly. He also had a dry cough, dyspnoea and anaemia. Pneumonia caused by Pneumocystis carinii and human immunodeficiency virus (HIV) infection were documented. The HIV infection was confirmed in 1997 with 290,000 virus copies. The patient had been in the Mexican State of Chiapas which is known to be endemic for visceral leishmaniosis (VL) and localized cutaneous leishmaniosis (LCL). The visceral symptoms were diagnosed as VL and the causal agent was identified as Leishmania (L.) mexicana. Identification of Leishmania was carried out by the analysis of amplified DNA with specific primers belonging to the Leishmania subgenus and by dot blot positive hybridisation of these polymerase chain reaction derived products with kDNA from the L. (L.) mexicana MC strain used as probe. This is the first case in Mexico of VL caused by a species of Leishmania that typically produces a cutaneous disease form.
Characterization of the SNAG and SLUG Domains of Snail2 in the Repression of E-Cadherin and EMT Induction: Modulation by Serine 4 Phosphorylation
Patricia Molina-Ortiz, Ana Villarejo, Matthew MacPherson, Vanesa Santos, Amalia Montes, Serhiy Souchelnytskyi, Francisco Portillo, Amparo Cano
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0036132
Abstract: Snail1 and Snail2, two highly related members of the Snail superfamily, are direct transcriptional repressors of E-cadherin and EMT inducers. Previous comparative gene profiling analyses have revealed important differences in the gene expression pattern regulated by Snail1 and Snail2, indicating functional differences between both factors. The molecular mechanism of Snail1-mediated repression has been elucidated to some extent, but very little is presently known on the repression mediated by Snail2. In the present work, we report on the characterization of Snail2 repression of E-cadherin and its regulation by phosphorylation. Both the N-terminal SNAG and the central SLUG domains of Snail2 are required for efficient repression of the E-cadherin promoter. The co-repressor NCoR interacts with Snail2 through the SNAG domain, while CtBP1 is recruited through the SLUG domain. Interestingly, the SNAG domain is absolutely required for EMT induction while the SLUG domain plays a negative modulation of Snail2 mediated EMT. Additionally, we identify here novel in vivo phosphorylation sites at serine 4 and serine 88 of Snail2 and demonstrate the functional implication of serine 4 in the regulation of Snail2-mediated repressor activity of E-cadherin and in Snail2 induction of EMT.
Montes de Oca,Nivian; Arévalos,Jersys; Nu?ez,Amalia; Riverón,Yamilka; Villoch,Alejandra; Hidalgo-Díaz,L;
Revista de Protecci?3n Vegetal , 2009,
Abstract: the national center of animal and plant health has a technology for manufacturing an agriculture bionematicide known as klamic. it is obtained from the strain of the fungus sd 187 imi pochonia chlamydosporia var. catenulata (kamyscho ex barron and onions) zare and w. gams, which is a biological control agent of root-knot nematodes. for five years, 98 batches of 25 kg at pilot scale have been produced, representing more than 1500 kg of product. the quality indicators of the product klamic have remained within the ranges established for the product with an average concentration of 1,6x107 chlamydospore.g of substrate-1, 91,32% viability of chlamydospores, 77,04% parasitism of m. incognita eggs, 5,43% water content and a concentration of micro-pollutants of 8,1x104 cfu.g of product -1. the application of the fermentation technology in solid state in polypropylene bags under the fulfilment of good manufacturing practices has enabled the obtaining of a quality inoculum, mainly used in characterization studies for toxicological and ecotoxicological testing, formulation, as well as in applications in sheltered crops and intensive orchards to demonstrate the capabilities of the fungus to settle in the soil and reduce nematode populations.
Montes de Oca,Nivian; Gonzáles,Rosa A; Riverón,Yamilka; Nu?ez,Amalia; Villoch,Alejandra; Rodríguez,Norbis;
Revista de Salud Animal , 2008,
Abstract: the use of the microorganisms has been a key when facing and solving serious problems in humanity. this study involves the use of living cultures, and if their importance is proved, they should be maintained and preserved in a collection. for the establishment and operation of such collection at the national center for animal and plant health (censa), the requirements of the world federation culture collection (wfcc, 1999) iso 9001:2000, the regulations of the good manufacture and laboratory practices, as well as the government dispositions for the management of biological agents were used. such establishment allowed the development of aspects like: documentation, identification, authenticity, purity, viability and stability of strains; maintenance of equipment; procedures supplying and exchanging strains; long term security; control of the environmental conditions; audits; fulfilment of the legislations; staff; training and biosafety. practical recommendations have been offered for the people in charge of collections of microorganisms and for the establishment of a quality system that assures the management of biological agents which participate in the research, production and service processes offered by the scientific institution of the country.
Cutaneous leishmaniasis caused by members of Leishmania braziliensis complex in Nayarit, State of Mexico
Sanchez-Tejeda, Gustavo;Rodríguez, Noris;Parra, Carlos I;Hernandez-Montes, Omar;C Barker, Douglas;Monroy-Ostria, Amalia;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000100002
Abstract: an epidemiological study was carried out in the northern mexican state, nayarit. fourteen patients with possible cutaneous leishmaniasis skin lesions gave positive montenegro skin tests. biopsies were taken from the skin ulcer and analyzed by polymerase chain reaction (pcr) with specific primers for the leishmania mexicana complex; however all biopsies were not amplified. pcr carried out with specific primers for the l. braziliensis complex resulted in the amplification of all patient dna. dna from 12 out of 14 biopsies gave positive amplification with primers species specific for l. (viannia) braziliensis and hybridized with a species specific l. (v.) braziliensis probe. these results demonstrate the presence in nayarit of at least two members of the l. braziliensis complex. most of the cutaneous lesions were caused by l. (v.) braziliensis and two by another species belonging to the l. braziliensis complex. as far as we are aware, this is the first report of l. (v.) braziliensis in nayarit. the main risk factor associated with the contraction of this disease in nayarit is attributed to working on coffee plantations.
E47 and Id1 Interplay in Epithelial-Mesenchymal Transition
Eva Cubillo, Antonio Diaz-Lopez, Eva P. Cuevas, Gema Moreno-Bueno, Hector Peinado, Amalia Montes, Vanesa Santos, Francisco Portillo, Amparo Cano
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0059948
Abstract: E12/E47 proteins (encoded by E2A gene) are members of the class I basic helix-loop-helix (bHLH) transcription factors (also known as E proteins). E47 has been described as repressor of E-cadherin and inducer of epithelial-mesenchymal transition (EMT). We reported previously that EMT mediated by E47 in MDCK cells occurs with a concomitant overexpression of Id1 and Id3 proteins. Id proteins belong to class V of HLH factors that lack the basic domain; they dimerise with E proteins and prevent their DNA interaction, thus, acting as dominant negative of E proteins. Here, we show that E47 interacts with Id1 in E47 overexpressing MDCK cells that underwent a full EMT as well as in mesenchymal breast carcinoma and melanoma cell lines. By conducting chromatin immunoprecipitation assays we demonstrate that E47 binds directly to the endogenous E-cadherin promoter of mesenchymal MDCK-E47 cells in a complex devoid of Id1. Importantly, our data suggest that both E47 and Id1 are required to maintain the mesenchymal phenotype of MDCK-E47 cells. These data support the collaboration between E47 and Id1 in the maintenance of EMT by mechanisms independent of the dominant negative action of Id1 on E47 binding to E-cadherin promoter. Finally, the analysis of several N0 breast tumour series indicates that the expression of E47 and ID1 is significantly associated with the basal-like phenotype supporting the biological significance of the present findings.
Analysis of Kinetoplast DNA from Mexican Isolates of Leishmania (L.) mexicana
Omar Hernández-Montes,Saúl González Guzmán,Federico Martínez Gómez,Douglas C. Barker,Amalia Monroy-Ostria
Interdisciplinary Perspectives on Infectious Diseases , 2012, DOI: 10.1155/2012/279081
Abstract: This study analyzed DNA minicircles of Mexican isolates of L. (Leishmania) mexicana to look for genetic differences between strains isolated from patients with diffuse cutaneous (DCL) and localized (LCL) leishmaniasis. The kDNA was analyzed using polymerase chain reaction (PCR), restriction fragment polymorphism analysis of the PCR products (PCR-RFLP) and the PCR products were sequenced. In the PCR with primers specific for the subgenus Leishmania, the Mexican isolates gave higher amplification products than the other L. mexicana complex strains and with specific primers for the L. mexicana complex they were poorly amplified. In the PCR-RFLP analysis with the Eco RV, Hae III, and Mbo I endonucleases, the Mexican isolates displayed similar restriction patterns, but different from the patterns of the other members of the L. mexicana complex. In the phylogenetic tree constructed, the kDNA sequences of the Mexican clones formed two groups including sequences of LCD or LCL clones, apart from the other L. mexicana complex members. These results suggest that the kDNA minicircles of the Mexican isolates are more polymorphic than the kDNA of other members of the L. mexicana complex and have different recognition sites for the restriction enzymes used in this study. 1. Introduction Cutaneous leishmaniasis (CL) is the most widespread form of leishmaniasis, causing primary localized skin lesions (LCL) from which parasites can disseminate to the nasopharyngeal mucosa and cause the disfiguring lesions typical of mucocutaneous leishmaniasis (MCL) or disseminated to the entire body as nodular lesions (DCL) [1]. American cutaneous leishmaniasis is characterized by a spectrum of clinical presentations. These include LCL caused by L. (Leishmania) mexicana, DCL caused by L. (Leishmania) amazonensis, L. (Leishmania) venezuelensis, and L. (Leishmania) pifanoi, all belonging to the Leishmania mexicana complex, and MCL caused by members of the Leishmania braziliensis complex [2]. Cutaneous leishmaniasis in Mexico is highly endemic with broad geographical distribution of the different clinical manifestations. In endemic areas, LCL or MCL can be caused by members of both L. mexicana and L. braziliensis complexes [3], making more accurate analysis and identification of the Leishmania strains imperative so that opportune and appropriate treatment can be administered. Polymerase chain reaction (PCR) approaches have been used in parasite DNA analysis. Several PCR molecular targets have been developed, including minicircle kinetoplast DNA (kDNA) [3], the miniexon (spliced leader RNA)
Cytosolic phospholipase A2 S-nitrosylation in ghrelin protection against detrimental effect of ethanol cytotoxicity on gastric mucin synthesis ——Ghrelin in gastric mucosal protection  [PDF]
Bronislaw L. Slomiany, Amalia Slomiany
Health (Health) , 2010, DOI: 10.4236/health.2010.29152
Abstract: Ghrelin, a peptide hormone produced mainly in the stomach, has emerged recently as an important regulator of nitric oxide synthase (NOS) and cyclooxygenase (COX) enzyme systems, the products of which play direct cytoprotective function in the maintenance of gastric mucosal integrity. In this study, using gastric mucosal cells, we report on the role of ghrelin in countering the cytotoxic effect of ethanol on mucin synthesis. We show that the countering effect of ghrelin on mucin synthesis was associated with the increase in NO and PGE2 production, and characterized by a marked up-regulation in cytosolic phospholipase A2 (cPLA2) activity. The ghrelin-induced up-regulation in mucin synthesis, like that of cPLA2 activity, was subject to suppression by Src inhibitor, PP2 and ERK inhibitor, PD98059, as well as ascorbate. Moreover, the loss in countering effect of ghrelin on the ethanol cytotoxicity and mucin synthesis was attained with cNOS inhibitor, L-NAME as well as COX-1 inhibitor SC-560. The effect of L-NAME was reflected in the inhibition of ghrelin-induced mucosal cell capacity for NO production, cPLA2 S-nitrosylation and PGE2 generation, whereas COX-1 inhibitor caused only the inhibition in PGE2 generation. Our findings suggest that the activation of gastric mucosal cPLA2 through cNOS-induced S-nitrosylation plays an essential role in the countering effect of ghrelin on the disturbances in gastric mucin synthesis caused by ethanol cytotoxicity.
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