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Search Results: 1 - 10 of 336383 matches for " Almir S Martins "
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From Rat to Human: Regulation of Renin-Angiotensin System Genes by Sry
Jeremy W. Prokop,Ingrid Kazue Mizuno Watanabe,Monte E. Turner,Adam C. Underwood,Almir S. Martins,Amy Milsted
International Journal of Hypertension , 2012, DOI: 10.1155/2012/724240
Abstract: The testis determining protein, Sry, has functions outside of testis determination. Multiple Sry loci are found on the Y-chromosome. Proteins from these loci have differential activity on promoters of renin-angiotensin system genes, possibly contributing to elevation of blood pressure. Variation at amino acid 76 accounts for the majority of differential effects by rat proteins Sry1 and Sry3. Human SRY regulated rat promoters in the same manner as rat Sry, elevating Agt, Ren, and Ace promoter activity while downregulating Ace 2. Human SRY significantly regulated human promoters of AGT, REN, ACE2, AT2, and MAS compared to control levels, elevating AGT and REN promoter activity while decreasing ACE2, AT2, and MAS. While the effect of human SRY on individual genes is often modest, we show that many different genes participating in the renin-angiotensin system can be affected by SRY, apparently in coordinated fashion, to produce more Ang II and less Ang-(1–7).
Genomic and expression analysis of multiple Sry loci from a single Rattus norvegicus Y chromosome
Monte E Turner, Carey Martin, Almir S Martins, Jeffrey Dunmire, Joel Farkas, Daniel L Ely, Amy Milsted
BMC Genetics , 2007, DOI: 10.1186/1471-2156-8-11
Abstract: Y chromosome fragments were amplified and sequenced using primers that included the entire Sry coding region and flanking sequences. The analysis of these sequences identified six Sry loci on the Y chromosome. These are paralogous copies consistent with a single phylogeny and the divergence between any two copies is less than 2%. All copies have a conserved reading frame and amino acid sequence consistent with function. Fragment analysis of genomic DNA showed close approximations of experimental with predicted values, validating the use of this method to identify proportions of each copy. Using the fragment analysis procedure with cDNA samples showed the Sry copies expressed were significantly different from the genomic distribution (testis p < 0.001, adrenal gland p < 0.001), and the testis and adrenal copy distribution in the transcripts were also significantly different from each other (p < 0.001). Total Sry transcript expression, analyzed by real-time PCR, showed significantly higher levels of Sry in testis than adrenal gland (p, 0.001).The SHR Y chromosome contains at least 6 full length copies of the Sry gene. These copies have a conserved coding region and conserved amino acid sequence. The pattern of divergence is not consistent with gene conversion as the mechanism for this conservation. Expression studies show multiple copies expressed in the adult male testis and adrenal glands, with tissue specific differences in expression patterns. Both the DNA sequence analysis and RNA transcript expression analysis are consistent with more than one copy having function and selection preventing divergence although we have no functional evidence.The recent analysis of the DNA sequence of the human Y chromosome has required a change from the classical view of the mammalian Y chromosome as a passive, genetic graveyard to one of an active, self correcting entity [1,2]. In reality, the mammalian Y chromosome is a combination of both the classical and modern views, since di
Canine distemper virus detection in asymptomatic and non vaccinated dogs
Del Puerto, Helen L.;Vasconcelos, Anilton C.;Moro, Luciana;Alves, Fabiana;Braz, Gissandra F.;Martins, Almir S.;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000200007
Abstract: a quantitative real time polymerase chain reaction (pcr) revealed canine distemper virus presence in peripheral blood samples from asymptomatic and non vaccinated dogs. samples from eleven domestic dogs with no signs of canine distemper and not vaccinated at the month of collection were used. canine distemper virus vaccine samples in vero cells were used as positive controls. rna was isolated with trizol?, and treated with a turbo dna-free kit. primers were designed for canine distemper virus nucleocapsid protein coding region fragment amplification (84 bp). canine b-actin (93 bp) was utilized as the endogenous control for normalization. quantitative results of real time pcr generated by abi prism 7000 sds software showed that 54.5% of dogs with asymptomatic canine distemper were positive for canine distemper virus. dissociation curves confirmed the specificity of the real time pcr fragments. this technique could detect even a few copies of viral rna and identificate subclinically infected dogs providing accurate diagnosis of this disease at an early stage.
From Rat to Human: Regulation of Renin-Angiotensin System Genes by Sry
Jeremy W. Prokop,Ingrid Kazue Mizuno Watanabe,Monte E. Turner,Adam C. Underwood,Almir S. Martins,Amy Milsted
International Journal of Hypertension , 2012, DOI: 10.1155/2012/724240
Abstract: The testis determining protein, Sry, has functions outside of testis determination. Multiple Sry loci are found on the Y-chromosome. Proteins from these loci have differential activity on promoters of renin-angiotensin system genes, possibly contributing to elevation of blood pressure. Variation at amino acid 76 accounts for the majority of differential effects by rat proteins Sry1 and Sry3. Human SRY regulated rat promoters in the same manner as rat Sry, elevating Agt, Ren, and Ace promoter activity while downregulating Ace 2. Human SRY significantly regulated human promoters of AGT, REN, ACE2, AT2, and MAS compared to control levels, elevating AGT and REN promoter activity while decreasing ACE2, AT2, and MAS. While the effect of human SRY on individual genes is often modest, we show that many different genes participating in the renin-angiotensin system can be affected by SRY, apparently in coordinated fashion, to produce more Ang II and less Ang-(1–7). 1. Introduction Many genes on the Y chromosome that are expressed in tissues not involved in testis formation could contribute to sex differences in blood pressure and other disease phenotypes. Sry is believed to have evolved from the X chromosome gene Sox3 during the process of Y-chromosome formation in therian mammals [1]. Since Sox3 has functions other than testis determination [2], Sry may also have additional functions outside testis determination. Sry and other Sox proteins are architectural transcription factors that bind to the minor groove of DNA, changing gene regulation through inducing a bend in the DNA [3]. The spontaneously hypertensive rat (SHR) has at least seven expressed Sry loci whereas the normotensive Wistar Kyoto (WKY) rat has at least six [4], lacking Sry3. Sry transcripts have been observed in adult rat tissues consistent with blood pressure regulation [5], and one or more of the Sry loci play a role in the development of hypertension in SHR [6]. Many rodent species have multiple Sry loci, while human and mouse have only one known locus. Sry proteins in human, rat, and other placental mammals have a homologous HMG box, hinge region, and bridge domain, with little homology in the N and C terminal ends (Figure 1(a)). The role of Sry regulation of blood pressure in humans has not been studied directly. However, with the high degree of conservation in the DNA-binding domain between human and rat, functions of Sry seen in rat are likely to translate into clinical relevance for human male blood pressure regulation. Figure 1: Modeling Sry variations of Sry1, Sry3, and hSRY. (a)
A Hermenêutica no Campo Organizacional: duas possibilidades interpretativistas de pesquisa Hermeneutic Questions in Organizational Studies: two interpretative possibilities for research Hermenéutica en el Campo Organizacional: dos posibilidades interpretativas en la investigación
VIEIRA, Almir Martins,RIVERA, Dario Paulo Barrera
Revista Brasileira de Gest?o de Negócios , 2012,
Abstract: RESUMO Este trabalho tem por objetivo apresentar considera es teóricas sobre dois tipos de estratégia de pesquisa: fenomenologia e etnografia. Com base na perspectiva hermenêutica, s o apresentadas proposi es conceituais das duas vertentes, sendo que a primeira é tratada no contexto sociológico e a segunda é apresentada em bases antropológicas. Seus usos similares no contexto organizacional – como também suas diferen as – s o evidenciados de acordo com as contribui es teóricas de autores pertencentes ao universo qualitativo da pesquisa organizacional. Destaca-se, pela apresenta o dos pressupostos teóricos relatados, a adequa o da proposta interpretativa para consecu o de pesquisas que tomam a organiza o como objeto de estudo, em busca da identifica o das formas de constru o da realidade social resultante da análise de significados e experiências vividas por seus participantes, ressaltando-se a importancia do envolvimento entre pesquisador e pesquisado. A discuss o teórica a respeito das duas vertentes permitiu notar a adequa o da prática de pesquisas fenomenológicas e etnográficas no campo de estudos organizacionais, manifestando-se como possibilidades metodológicas para identificar dinamicas que se atrelam à experiência de vida, favorecendo a análise do ser humano na condi o de fen meno de interpreta o. ABSTRACT The present text has the objective of presenting theoretical considerations regarding two types of strategy of research: phenomenology and ethnography. Based on a hermeneutic approach, the two conceptual propositions are presented, the first being dealt with in the sociological context, and the second presented as an anthropological basis. Their similar uses in an organizational context - as well as their differences – are highligthed according to the theoretical contributions of authors belonging to the qualitative universe of organizational research. The text reveals, by the presentations of reported theoretical assumptions, the relevance of the interpretative perspective for the conduction of research that has the organization as object of study, in the pursuit of the identification of ways of constructing the social reality that is the result of the analysis of meanings and experiences lived by the participants, highlighting the importance of the bond between researcher and the research object The theoretical discussion regarding the two perspectives allows to observe the competence of the phenomenological and ethnographic research practice within the field of organizational studies, showing their methodological possibilities to
An Integrated Approach for Process Control Valves Diagnosis Using Fuzzy Logic  [PDF]
Alvaro Luiz G. Carneiro, Almir C. S. Porto Jr.
World Journal of Nuclear Science and Technology (WJNST) , 2014, DOI: 10.4236/wjnst.2014.43019
Abstract:
Control valves are widely used in industry to control fluid flow in several applications. In nuclear power systems they are crucial for the safe operation of plants. Therefore, the necessity of improvements in monitoring and diagnosis methods started to be of extreme relevance, establishing as main goal of the reliability and readiness of the system components. The main focus of this work is to study the development of a model of non-intrusive monitoring and diagnosis applied to process control valves using artificial intelligence by fuzzy logic technique, contributing to the development of predictive methodologies identifying faults in incipient state. Specially in nuclear power plants, the predictive maintenance contributes to the security factor in order to diagnose in advance the occurrence of a possible failure, preventing severs situations. The control valve analyzed belongs to a steam plant which simulates the secondary circuit of a PWR—Pressurized Water Reactor. The maintenance programs are being implemented based on the ability to diagnose modes of degradation and to take measures to prevent incipient failures, improving plant reliability and reducing maintenance costs. The approach described in this paper represents an alternative departure from the conventional qualitative techniques of system analysis. The methodology used in this project is based on signatures analysis, considering the pressure (psi) in the actuator and the stem displacement (mm) of the valve. Once the measurements baseline of the control valve is taken, it is possible to detect long-term deviations during valve lifetime, detecting in advance valve failures. This study makes use of MATLAB language through the “fuzzy logic toolbox” which uses the method of inference “Mamdani”, acting by fuzzy conjunction, through Triangular Norms (t-norm) and Triangular Conorms (t-conorm). The main goal is to obtain more detailed information contained in the measured data, correlating them to failure situations in the incipient stage.
Canine distemper virus induces apoptosis in cervical tumor derived cell lines
Helen L Del Puerto, Almir S Martins, Amy Milsted, Elaine M Souza-Fagundes, Gissandra F Braz, Barbara Hissa, Luciana O Andrade, Fabiana Alves, Daniela S Raj?o, R?mulo C Leite, Anilton C Vasconcelos
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-334
Abstract: Apoptosis is a regulated form of cell death which occurs during physiological conditions. It plays a critical role in the homeostasis of multicelular organisms, and constitutes a common pathway for cell replacement, tissue remodeling, damaged cell removal and elimination of cancer cells [1]. It is a complex process which involves the participation of caspases, activation of proapoptotic genes, and inhibition of antiapoptotic proteins. Cells undergoing apoptosis present typical morphological characteristics, including membrane blebbing, chromatin condensation, cell shrinkage and apoptotic body formation [2].Apoptosis is triggered by sequential activation of caspases, a group of cysteine proteases, and proceeds primarily through two pathways. The extrinsic pathway involves activation of caspase-8 and is initiated by ligand interaction with death receptors, while the intrinsic pathway is activated by an imbalance between proapoptotic and antiapoptotic proteins from Bcl-2 family in mitochondria and cytosol, resulting in release of cytochrome c from mitochondria, which in turn activates caspase-9 [3]. Both caspase-8 and caspase-9 activate caspase-3, which along with other effectors caspases cleave critical cellular proteins, resulting in apoptosis [3].Many viral proteins can influence the cellular pathways that control cell proliferation and apoptosis. Some viral proteins trigger apoptotic cell death, and this may be important in host defense and viral spread. In other cases, viral proteins inhibit apoptosis [4].HeLa cells, derived from a cervical tumor, encode apoptosis inhibitor proteins E6 and E7, oncoproteins expressed by high-risk human papillomavirus (HPV), among them the HPV18 type [5,6]. HPV E6 protein target p53, a tumor suppressor protein that regulates the cell cycle. The E6 protein binding to p53 causes p53 inactivation by its degradation, turning off its function [6,7]. On the other hand, the HPV E7 protein acts by binding to members of the Rb (retinoblastom
TRANSMISS O DE CóDIGO DE BARRAS POR RáDIO FREQUêNCIA USANDO MICROCONTROLADORES
Alexandre S. Gonsalves,Almir O. Artero
Colloquium Exactarum , 2010, DOI: 10.5747/ce.2010.v02.n2.e014
Abstract: This paper presents a wireless barcode system reader, which can, advantageously, replace the wired system, used in most applications. Using a microcontroller to do all the processing, the proposed system transmits the information collected by a simple wire barcode reader with a common thread to a computer using radio frequency signals. With a low cost, the proposed project allows its use in various applications that need to collect data, mainly, in areas of difficult access, or when it is impossible to bring the object into the reader connected to the wired computer.
Principales criaderos para Aedes aegypti y culícidos asociados, Argentina
Stein,Marina; Oria,Griselda Inés; Almirón,Walter Ricardo;
Revista de Saúde Pública , 2002, DOI: 10.1590/S0034-89102002000600013
Abstract: breeding containers for aedes (stegomyia) aegypti were identified in two cities of chaco province (northeast argentina): presidencia roque saenz pe?a and machagai. all water-retaining recipients found in house backyards capable to retain water were classified according to their type and size, counted and checked. aedes aegypti and culex quinquefasciatus were the most frequently collected species, being also found cx. maxi, cx. saltanensis and ochlerotatus scapularis. tires and car batteries represented the most important type of container where immature forms of culicids could be found. rain was an important factor for ae. aegypti proliferation, as well as the widespread habit of the population of keeping useless containers at home, which allows the development of culicids.
Principales criaderos para Aedes aegypti y culícidos asociados, Argentina
Stein Marina,Oria Griselda Inés,Almirón Walter Ricardo
Revista de Saúde Pública , 2002,
Abstract: Se identificaron criaderos de Aedes (Stegomyia) aegypti en dos ciudades de la provincia del Chaco (Noreste de Argentina): Presidencia Roque Saenz Pe a y Machagai. Los recipientes encontrados en los patios de las viviendas capaces de colectar agua se clasificaron según tipo y tama o, se contaron y examinaron. Aedes aegypti y Culex quinquefasciatus fueron las especies mas abundantes, encontrándose además Cx. maxi, Cx. saltanensis y Ochlerotatus scapularis. Los neumáticos y cajas de baterías para autos fueron los recipientes que más aportaron a la producción de formas inmaduras de los culícidos colectados. Las lluvias fueron un factor importante para la proliferacion de Ae. aegypti, así como también el habito comun en la población de guardar recipientes en sus casas que permitan el desarrollo de estos culícidos.
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