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Search Results: 1 - 10 of 13796 matches for " Alice Beatriz Mombach Pinheiro; "
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High prevalence of methicillin-resistant Staphylococcus aureus with SCCmec type III in cystic fibrosis patients in southern, Brazil
Reiter, Keli Cristine;Machado, Alice Beatriz Mombach Pinheiro;Freitas, Ana Lúcia Peixoto de;Barth, Afonso Luís;
Revista da Sociedade Brasileira de Medicina Tropical , 2010, DOI: 10.1590/S0037-86822010000400008
Abstract: introduction: bacterial colonization of the lungs is the main cause of morbidity in cystic fibrosis (cf). pathogens such as staphylococcus aureus are very well adapted to the pulmonary environment and may persist for years in the same patient. genetic determinants of these bacteria, such as the presence of sccmec have recently emerged as a problem in this population of patients. methods: staphylococcus aureus isolates obtained from different clinical materials coming from cf and non-cf patients attended at a cystic fibrosis reference hospital were compared according to sccmec type and antibiotic susceptibility profile. results: three hundred and sixty-four single-patient staphylococcus aureus isolates were collected, of which 164 (45%) were from cf patients. among the latter, 57/164 (44.5%) were mrsa, and among the non-cf patients, 89/200 (35%) were mrsa. associated pathogens were found in 38 cf patients. all 57 mrsa from cf patients harbored the multiresistant cassette type iii. in contrast, 31/89 mrsa from non-cf patients harbored sccmec type i (35%) and 44/89 harbored type iii (49%). the antibiotic susceptibility pattern was similar between cf and non-cf patients. conclusions: the high prevalence of multiresistant sccmec type iii among cf patients compared with non-cf patients in our institution may make it difficult to control disease progression through antibiotic therapy for promoting the survival of this kind of patient.
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
de-Paris, Fernanda;Machado, Alice Beatriz Mombach Pinheiro;Gheno, Tailise Conte;Ascoli, Bruna Maria;Oliveira, Kátia Ruschel Pilger de;Barth, Afonso Luis;
Brazilian Journal of Infectious Diseases , 2011, DOI: 10.1590/S1413-86702011000400004
Abstract: streptococcus agalactiae or group b streptococcus (gbs) is one of the most important causal agents of serious neonatal infections. numerous assays have been evaluated for gbs screening in order to validate a fast and efficient method. the aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (pcr) with specific primers (atr gene). two hundred and sixty-three samples were analyzed. vaginal samples were collected, according to the centers for disease control and prevention (cdc) recommendations, from women over 35 weeks of pregnancy at hospital de clínicas de porto alegre (hcpa). two different extraction methods were tested in all samples collected. pcr technique yielded 71 (26.99%) positive results. sensitivity and specificity for pcr were 100% and 86.88%, respectively. pcr demonstrated a shorter turnaround time than the culture. the molecular methodology proved to be a useful screening for gbs, allowing effective treatment to be initiated in shorter time to prevent newborn infection.
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates
Coutinho, Vivian de Lima Spode;Paiva, Rodrigo Minuto;Reiter, Keli Cristine;de-Paris, Fernanda;Barth, Afonso Luis;Machado, Alice Beatriz Mombach Pinheiro;
Brazilian Journal of Infectious Diseases , 2010, DOI: 10.1590/S1413-86702010000600004
Abstract: introduction: resistance to macrolides, lincosamides and streptogramins b (mlsb antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. the expression of mlsb resistance lead to three phenotypes, namely constitutive resistance (cmlsb), inducible resistance (imlsb), and resistance only to macrolides and streptogramins b (msb). the imlsb resistance is the most difficult to detect in the clinical laboratory. objective: this study investigated the expression of mlsb resistance and the prevalence of the erm genes among 152 clinical isolates of staphylococcus aureus and coagulase-negative staphylococcus (cns) from hospital de clínicas de porto alegre. methods: primary mlsb resistance was detected by the disk diffusion method. isolates with imlsb phenotype were tested by double-disk induction method. all isolates were tested by a genotypic assay, pcr with specific primers. results: a total of 46.7% of staphylococci were positive for cmlsb; 3.3% for imlsb and 3.3% for msb. one or more erm genes were present in 50.1% of isolates. the gene erma was detected in 49 isolates, ermc in 29 and ermb in 3. conclusion: the prevalence of the erma, ermb and ermc genes were 29.6%, 17.1% and 0.66% respectively, and constitutive resistance was the most frequent as compared to the other two phenotypes.
Comparison of the performance of polymerase chain reaction and pp65 antigenemia for the detection of human cytomegalovirus in immunosuppressed patients
Martiny, Patrícia Borba;de-Paris, Fernanda;Machado, Alice Beatriz Mombach Pinheiro;Mello, Ricardo Obalski de;Senger, Martha Bergman;Corrêa, Maria Clara Medina;Werres Junior, Luiz Carlos;Souza, Carolina Fischinger Moura de;
Revista da Sociedade Brasileira de Medicina Tropical , 2011, DOI: 10.1590/S0037-86822011005000029
Abstract: introduction: human cytomegalovirus (hcmv) is often reactive in latently infected immunosuppressed patients. accordingly, hcmv remains one of the most common infections following solid organ and hemopoietic stem cell transplantations, resulting in significant morbidity, graft loss and occasional mortality. the early diagnosis of hcmv disease is important in immunosuppressed patients, since in these individuals, preemptive treatment is useful. the objective of this study was to compare the performance of the in-house qualitative polymerase chain reaction (pcr) and pp65 antigenemia to hcmv infection in immunosuppressed patients in the hospital de clínicas of porto alegre (hcpa). methods: a total of 216 blood samples collected between august 2006 and january 2007 were investigated. results: among the samples analyzed, 81 (37.5%) were hcmv-positive by pcr, while 48 (22.2%) were positive for antigenemia. considering antigenemia as the gold standard, sensitivity, specificity, positive predictive values and negative predictive values for pcr were 87.5%, 76.8%, 51.8% and 95.5% respectively. conclusions: these results demonstrated that qualitative pcr has high sensitivity and negative predictive value (npv). consequently pcr is especially indicated for the initial diagnosis of hcmv infection. in the case of preemptive treatment strategy, identification of patients at high-risk for hcmv disease is fundamental and pcr can be useful tool.
Prevalência de infec??o congênita por citomegalovírus em recém-nascidos de uma unidade de tratamento intensivo de um hospital público
Miura, Clarissa Schreiner;Miura, Ernani;Mombach, Alice Beatriz;Cheskya, Marisa;
Jornal de Pediatria , 2006, DOI: 10.2223/JPED.1436
Abstract: objective: to determine the prevalence of congenital cytomegalovirus infection in newborn infants admitted to an intensive care unit in a public hospital in porto alegre. methods: a cross-sectional study of 261 newborn infants born at a public hospital in the city of porto alegre in 2003 and admitted to the intensive care ward. urine samples were collected within 7 days of birth and a polymerase chain reaction-pcr performed to test for cytomegalovirus dna. results: the prevalence of congenital cytomegalovirus infection among the study population was 0.8% (95% ci: 0.097%-2.86%). it was not possible to assess risk factors because this prevalence was so low. conclusions: the prevalence of congenital cytomegalovirus infection in an intensive care unit at a public hospital in porto alegre was not considered elevated and was comparable with prevalence rates found by other studies.
Prevalência de infec o congênita por citomegalovírus em recém-nascidos de uma unidade de tratamento intensivo de um hospital público
Miura Clarissa Schreiner,Miura Ernani,Mombach Alice Beatriz,Cheskya Marisa
Jornal de Pediatria , 2006,
Abstract: OBJETIVO: Determinar a prevalência da infec o congênita por citomegalovírus em recém-nascidos internados na unidade de terapia intensiva neonatal de um hospital público de Porto Alegre. METODOS: Estudo transversal, incluindo 261 recém-nascidos que nasceram em um hospital público da cidade de Porto Alegre no ano de 2003 e foram internados na unidade de terapia intensiva neonatal. Foi coletada amostra de urina nos primeiros 7 dias de vida e realizado o teste de rea o em cadeia da polimerase para a pesquisa do DNA do citomegalovírus. RESULTADOS: A prevalência de infec o congênita por citomegalovírus na popula o estudada foi de 0,8% (IC 95%: 0,097-2,86). Devido à baixa prevalência, n o foi possível associar fatores de risco. CONCLUS ES: A prevalência de infec o congênita por citomegalovírus em uma unidade de terapia intensiva neonatal de um hospital público de Porto Alegre n o foi considerada elevada, sendo semelhante à prevalência encontrada em outros estudos realizados.
Polymerase chain reaction as a useful and simple tool for rapid diagnosis of tuberculous meningitis in a Brazilian tertiary care hospital
Dora, José Miguel;Geib, Guilherme;Chakr, Rafael;Paris, Fernanda de;Mombach, Alice Beatriz;Lutz, Larissa;Souza, Carolina Fischinger Moura de;Goldani, Luciano Z.;
Brazilian Journal of Infectious Diseases , 2008, DOI: 10.1590/S1413-86702008000300015
Abstract: meningitis is a severe and potentially fatal form of tuberculosis. the diagnostic workup involves detection of acid-fast bacilli (afb) in the cerebrospinal fluid (csf) by microscopy or culture, however, the difficulty in detecting the organism poses a challenge to diagnosis. the use of the polymerase chain reaction (pcr) in the diagnostic approach to mycobacterium tuberculosis (mtb) meningitis has been reported as a fast and accurate method, with several commercial kits available. as an alternative, some institutions have been developing inexpensive in house assays. in our institution, we use an in house pcr for tuberculosis. we analyzed the performance of our pcr for the diagnosis of mtb meningitis in 148 consecutive patients, using mtb culture as gold standard. the sensitivity and specificity of csf pcr for the diagnosis of mtb meningitis was 50% and 98.6% respectively with a concordance with csf mycobacterial culture of 96% (kappa=0.52). in contrast to csf cultures for mtb, our pcr test is a fast, simple and inexpensive tool to diagnose tuberculous meningitis with a performance similar to that obtained with the available commercial kits.
Beatriz Pinheiro Arraes
Nuances : Estudos sobre Educa??o , 2011,
Abstract: Trata-se de um estudo do texto dramático Otelo: o Mouro de Veneza de autoria de William Shakespeare e traduzido por de Jean Melville e do texto narrativo Otelo, inspirado nessa mesma pe a, a adaptado por Hildegard Feist. Exploram-se os tra os que vinculam as duas composi es, seja no que se refere à forma como s o apresentadas, seja no tocante à carga conteudística. Assim exploramos conceitua es de texto ao lado do estudo dos fatores da obra como experiência humana e da sua permanência através dos séculos além do revestimento ideológico dado ao longo da história. Uma vez que nos interessa recuperar a recep o de clássicos como os de Shakespeare, este trabalho tem por objetivo analisar a adapta o para o público juvenil e de que forma esta contribui para a forma o de um público leitor do mesmo autor.
Os desafios e as preocupa es da escola atual
Heloisa Beatriz Alice Rubman
Pro-Posi??es , 2010, DOI: 10.1590/s0103-73072010000100016
G?del and the Incompleteness of Arithmetic  [PDF]
Advances in Pure Mathematics (APM) , 2016, DOI: 10.4236/apm.2016.68042
Abstract: People normally believe that Arithmetic is not complete because GÖdel launched this idea a long time ago, and it looks as if nobody has presented sound evidence on the contrary. We here intend to do that perhaps for the first time in history. We prove that what Stanford Encyclopedia has referred to as Theorem 3 cannot be true, and, therefore, if nothing else is presented in favour of GÖdel’s thesis, we actually do not have evidence on the incompleteness of Arithmetic: All available evidence seems to point at the extremely opposite direction.
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