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Search Results: 1 - 10 of 1482 matches for " Aleksandra Ellert-Miklaszewska "
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Characteristics of the Alternative Phenotype of Microglia/Macrophages and its Modulation in Experimental Gliomas
Konrad Gabrusiewicz, Aleksandra Ellert-Miklaszewska, Maciej Lipko, Malgorzata Sielska, Marta Frankowska, Bozena Kaminska
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0023902
Abstract: Microglia (brain resident macrophages) accumulate in malignant gliomas and instead of initiating the anti-tumor response, they switch to a pro-invasive phenotype, support tumor growth, invasion, angiogenesis and immunosuppression by release of cytokines/chemokines and extracellular matrix proteases. Using immunofluorescence and flow cytometry, we demonstrate an early accumulation of activated microglia followed by accumulation of macrophages in experimental murine EGFP-GL261 gliomas. Those cells acquire the alternative phenotype, as evidenced by evaluation of the production of ten pro/anti-inflammatory cytokines and expression profiling of 28 genes in magnetically-sorted CD11b+ cells from tumor tissues. Furthermore, we show that infiltration of implanted gliomas by amoeboid, Iba1-positive cells can be reduced by a systematically injected cyclosporine A (CsA) two or eight days after cell inoculation. The up-regulated levels of IL-10 and GM-CSF, increased expression of genes characteristic for the alternative and pro-invasive phenotype (arg-1, mt1-mmp, cxcl14) in glioma-derived CD11b+ cells as well as enhanced angiogenesis and tumor growth were reduced in CsA-treated mice. Our findings define for the first time kinetics and biochemical characteristics of glioma-infiltrating microglia/macrophages. Inhibition of the alternative activation of tumor-infiltrating macrophages significantly reduced tumor growth. Thus, blockade of microglia/macrophage infiltration and their pro-invasive functions could be a novel therapeutic strategy in malignant gliomas.
Application of immobilized cell preparation obtained from biomass of Gluconacetobacter xylinus bacteria in biotransformation of glycerol to dihydroxyacetone
Lidia Stasiak-Ró?ańska,Stanis?aw B?a?ejak,Aleksandra Miklaszewska
Acta Scientiarum Polonorum : Technologia Alimentaria , 2011,
Abstract: Introduction. Dihydroxyacetone (DHA), being a product of glycerol oxidation by acetic acid bacteria, is an important compound widely applied in the cosmetic, food, and pharmaceutical industry, as well as in medicine. Biotransformation of glycerol to DHA is catalyzed by glycerol dehydrogenase (GlyDH, EC bound with the cytoplasmic membrane of bacteria. An attempt was undertaken in this study to conduct glycerol biotransformation with immobilized fractions of a cell preparation with GlyDH activity. The content of dihydroxyacetone obtained with the cell preparation was compared with its content achieved in the reaction with immobilized viable cells of G. xylinus. Material and methods. Cell walls of Gluconacetobacter xylinus bacteria were disintegrated enzymatically. The resultant preparation was immobilized on calcium alginate or first separated into two fractions (precipitate and supernatant) by centrifugation and then immobilized. DHA content was determined colorimetrically after the reaction with 3,5-dinitrosalicilic acid. Glycerol content was assayed with the refractometric method. Results. After 20 days of the process, the concentration of DHA obtained with immobilized whole cells reached 25 g/l. In turn, the content of DHA obtained in the same period with immobilized fractions of the cell preparation accounted for 16.9 g/l and 8.95 g/l (depending on the fraction applied). Conclusions. DHA may be obtained in the process independent of G. xylinus metabolic activity using a preparation which displays the catalytic activity of glycerol dehydrogenase and obtained as a result of disintegration of live bacterial cells. The application of such a preparation may in the future eliminate technological problems posed by the presence of bacterial cells and their metabolites in the culture medium.
Joanna Miklaszewska
Studia Humanistyczne AGH , 2012,
Fatal Urosepsis: A 41 Year-Old Pregnant Woman—Case Report  [PDF]
Malgorzata Gosciniak, Dariusz Kawecki, Malgorzata Miklaszewska, Zenon Truszewski, Tomasz Lazowski, Miroslaw Wielgos, Piotr Radziszewski
Open Journal of Urology (OJU) , 2014, DOI: 10.4236/oju.2014.411024
Abstract: Introduction: Urinary tract infections are one of the most frequent infectious complications during pregnancy [1], while acute pyelonephritis is the main non-obstetric reason for hospitalisation [2] [3]and septic shock in pregnant women. Objectives and methods: The aim of our study is to analyse the diagnostics and treatment of a 41-year-old pregnant woman with the most severe form of urinary tract infection—urosepsis. Case report: A 41-year old woman in the 12th week of pregnancy hospitalised at Intensive Care Unit (ICU) after urological intervention (JJ stent). On the first day after the intervention, the patient was diagnosed as having urosepsis. Scores: SOFA 14 pts, APACHE II 26 pts, SAPS II SCORE 61 pts. Second day in ICU: spontaneous abortion. Microbiological investigation: Escherichia coli—extended-spectrum beta-lactamase (ESBL+). Conclusions: Apregnant patient with a complicated infection of the urinary system ought to be treated in a multi-profile hospital, offering a possibility to consult doctors of various specialisations (urologist, gynaecologist, anaesthesiologist, nephrologist) as well as full access to a radiology laboratory, which will ensure the choice of appropriate and safe treatment for both the mother and the developing foetus.
Sleep Duration and Sleep Problems in a Representative Sample of German Children and Adolescents  [PDF]
Angelika A. Schlarb, Marco D. Gulewitsch, Victoria Weltzer, Ute Ellert, Paul Enck
Health (Health) , 2015, DOI: 10.4236/health.2015.711154
Abstract: Objective: The main purpose of the present study was to evaluate sleep duration for nighttime sleep from early infancy to late adolescence in a German sample to illustrate the developmental course and age-specific variability of these variables among subjects. Methods: A total of 17,641 subjects from the KiGGS study were evaluated. The questionnaires contained questions about physical health, living circumstances, health behavior and risks, health supply, mental health, health-related quality of life and sleep. KiGGS assessed sleep by using parent questionnaire of children aged 0 to 10 years (n = 9944) and self-reports of adolescents (n = 7697) aged 11 to 17 years. Results: Total sleep duration decreased from 14.28 hours (SD = 2.33) at the ages 0 - 0.5 to 9.50 hours (SD = 0.82) at the ages of 10.5 - 11. Above the age of 11 adolescents report a decrease of sleep at night from 9.41 hours (SD = 1.33) at the age of 11 - 11.5 to 7.42 (SD = 1.73) at the age of 17.5 - 18 years. Unspecified sleep problems were reported of 19.5%, 13.0% of the children had difficulties falling asleep, difficulties sleeping through the night showed 8.8% of the children, whereas 3.0% report both symptoms—difficulties falling asleep and difficulties sleeping through the night. Conclusions: Age-specific variability of sleep duration is reported as well as sleep difficulties from infancy to adolescence. This is important knowledge for the health care professional who deals with sleep problems in pediatric practice.
Patchwork or matrix: Testing the capacity of the contemporary city
Stupar Aleksandra,?uki? Aleksandra
Spatium , 2007, DOI: 10.2298/spat0716001s
Abstract: Being exposed to the multiple needs of their contemporary users, the cities from all over the world have been forced to activate all capacities in order to intensify their land use, adjust their urban structure and reinvent some forgotten segments (ex-industrial areas, traffic nodes, docks, waterfronts) as generators of multilayered transformations and mutations. Therefore, the purpose of this paper is to analyze and compare different approaches of this practice, as well as to emphasize the relation between the applied global imperatives/trends/myths, local conditions and limitations, and the outcomes. Consequently, the first part will be structured around four key-words which often "justify" and activate multifunctional and intensive land use - connectivity, profit, identity and sustainability. The second part will be focused on the case of Serbia, describing a unique postmodern example of the fusion of local and global influences. The multifunctional land use in this case is a result of numerous political and economic problems, the plurality of values and (il)legal transformations of urban structure. This condition has finally affected the rising need for urban redesign, the re-organization and revitalization of city centers, fringe areas, devastated and degraded urban zones, as well as the development of existing transport and communication networks i.e. improvement of urban and regional connectivity. Additionally, the "model of territorial values" will be explained as a planning tool which could be used in order to increase the level of urban attractiveness, define urban parameters and estimate land values. .
Insulinooporno w cukrzycy typu 1
Aleksandra Uruska,Aleksandra Araszkiewicz
Pediatric Endocrinology, Diabetes and Metabolism , 2009,
Abstract: Insulina warunkuje transport glukozy do wi kszo ci komórek ustroju. Zmniejszona odpowied komórek docelowych na dzia anie insuliny nosi nazw zjawiska insulinooporno ci. Na zjawisko to wp ywaj zarówno czynniki genetyczne jak i rodowiskowe. Najistotniejszy wp yw na progresj insulinoopor-no ci ma styl ycia wykreowany przez post p cywilizacyjny. Wspó wyst powanie insulinooporno ci, oty o ci, cukrzycy, nadci nienia t tniczego i dysli-pidemii okre lane jest mianem zespo u metabolicznego. Zjawisko insulinooporno ci wi zano pocz tkowo jedynie z oty o ci i cukrzyc typu 2. Jednak kliniczne wyk adniki insulinooporno ci stwierdza si równie u wielu pacjentów z cukrzyc typu 1. Zmniejszon wra liwo tkanek na dzia anie insuliny czy si ze zmniejszon ilo ci miejsc wi zania insuliny oraz defektem jej postreceptorowego dzia ania. Wykazano, i zmniejszony wychwyt glukozy w ustroju, jest przede wszystkim nast pstwem zredukowanego wychwytu glukozy w mi niach szkieletowych. Istniej ró ne sposoby pomiaru insulino-oporno ci, jednak w ród chorych na cukrzyc typu 1 nie ka dy mo e mie zastosowanie. Z otym standardem oznaczania wra liwo ci tkanek na dzia anie insuliny jest metoda klamry hiperinsulinowej normoglikemicznej. Poszukuje si jednak innych sposobów oceny insulinooporno ci u pacjentów z cukrzyc typu 1, które by yby atwiej dost pne, prostsze i tańsze. W tym celu proponuje si wykorzystanie po rednich wyk adników oporno ci na dzia anie insuli-ny, koreluj cych z wynikami klamry euglikemicznej, takich jak: dane antropometryczne, cechy zespo u metabolicznego b d markery reakcji zapalnej. Zjawisko insulinooporno ci w cukrzycy typu 1 jest niezwykle istotnym problemem, poniewa zaobserwowano jej wp yw na rozwój przewlek ych powik ań schorzenia. Identyfikacja wyk adników insulinooporno ci w ród pacjentów z cukrzyc typu 1 jest niezb dna do uzyskania lepszego wyrównania metabo-licznego i profilaktyki przewlek ych powik ań schorzenia w tej grupie osób.
Comparison of BMI Derived from Parent-Reported Height and Weight with Measured Values: Results from the German KiGGS Study
Anna-Kristin Brettschneider,Ute Ellert,Angelika Schaffrath Rosario
International Journal of Environmental Research and Public Health , 2012, DOI: 10.3390/ijerph9020632
Abstract: The use of parent-reported height and weight is a cost-efficient instrument to assess the prevalence of children’s weight status in large-scale surveys. This study aimed to examine the accuracy of BMI derived from parent-reported height and weight and to identify potential predictors of the validity of BMI derived from parent-reported data. A subsample of children aged 2–17 years (n = 9,187) was taken from the 2003–2006 cross-sectional German KiGGS study. Parent-reported and measured height and weight were collected and BMI was calculated. Besides descriptive analysis, linear regression models with BMI difference and logistic regression models with weight status misclassification as dependent variables were calculated. Height differences varied by gender and were generally small. Weight and BMI were under-reported in all age groups, the under-reporting getting stronger with increasing age. Overall, the proportion for overweight and obesity based on parental and measured reports differed slightly. In the youngest age group, the proportion of overweight children was overestimated, while it was underestimated for older children and adolescents. Main predictors of the difference between parent reported and measured values were age, gender, weight status and parents’ perception of the child’s weight. In summary, the exclusive use of uncorrected parental reports for assessment of prevalence rates of weight status is not recommended.
Validity and predictors of BMI derived from self-reported height and weight among 11- to 17-year-old German adolescents from the KiGGS study
Anna-Kristin Brettschneider, Angelika Rosario, Ute Ellert
BMC Research Notes , 2011, DOI: 10.1186/1756-0500-4-414
Abstract: Self-reported and measured data were collected from a sub-sample (3,468 adolescents aged 11-17) from the German Health Interview and Examination Survey for Children and Adolescents (KiGGS). BMI was calculated from both reported and measured values, and these were compared in descriptive analyses. Linear regression models with BMI difference (self-reported minus measured) and logistic regression models with weight status misclassifications as dependent variables were calculated.Height was overestimated by 14- to 17-year-olds. Overall, boys and girls under-reported their weight. On average, BMI values calculated from self-reports were lower than those calculated from measured values. This underestimation of BMI led to a bias in the prevalence rates of under- and overweight which was stronger in girls than in boys. Based on self-reports, the prevalence was 9.7% for underweight and 15.1% for overweight. However, according to measured data the corresponding rates were 7.5% and 17.7%, respectively. Linear regression for BMI difference showed significant differences according to measured weight status: BMI was overestimated by underweight adolescents and underestimated by overweight adolescents. When weight status was excluded from the model, body perception was statistically significant: Adolescents who regarded themselves as 'too fat' underestimated their BMI to a greater extent. Symptoms of a potential eating disorder, sexual maturation, socio-economic status (SES), school type, migration background and parental overweight showed no association with the BMI difference, but parental overweight was a consistent predictor of the misclassification of weight status defined by self-reports.The present findings demonstrate that the observed discrepancy between self-reported and measured height and weight leads to inaccurate estimates of the prevalence of under- and overweight when based on self-reports. The collection of body perception data and parents' height and weight is t
Dekompozycja z o onych wykresów Poincare zmiennych hemodynamicznych
Przemys?aw Guzik,Jaros?aw Piskorski,Jerzy Ellert,Janusz Tarchalski
Polish Journal of Cardiology , 2006,
Abstract: Wprowadzenie: Ocena zmienno ci rytmu serca i ci nienia t tniczego znajduje zastosowanie w badaniach naukowych i pracy klinicznej. Ze wzgl du na ograniczenia techniczne analiza zmienno ci parametrów hemodynamicznych, takich jak: rzut serca (cardiac output - CO), obj to wyrzutowa (stroke volume - SV) czy systemowy opór naczyniowy (systemie vascular resistance - SVR) jest rzadko wykonywana. W ocenie zmienno ci rytmu serca wykorzystuje si m.in. wykresy Poincare opisywane takimi deskryptorami, jak: SD1, SD2, czy wspó czynnik r. Parametry te charakteryzuj zmienno krótko- (SD1) i d ugoterminow (SD2) oraz równowag , mi dzy nimi (wspó czynnik r). Cz sto wykresy Poincare utworzone dla d u szych rejestracji przyjmuj z o one kszta ty przypominaj ce np.: komet , podkow , para bol lub zgrupowanie wysp. Stosuj c metod dekompozycji, takz o one wykresy rozk ada si na mniejsze wykresy Poincare (mini-Poincare), co umo liwia dynamiczn ocen chwilowych zmian zachodz cych w autonomicznej modulacji pracy serca. Cel pracy: Przeprowadzenie dekompozycji z o onych wykresów Poincare SV, CO, SVR i odst pów RR pochodz cych z wielogodzinnej ci g ej rejestracji parametrów hemodynamicznych. Materia i metodyka: Badanie zrealizowano wykorzystuj c oko o 19-godzinne nagranie z danymi hemodynamicznymi 72 356 kolejnych uderzeń serca. Rejestracj hemodynamiczn wykonano u 49-letniego chorego ze wie ym zawa em serca po skutecznej angioplastyce prawej t tnicy wieńcowej, wykorzystuj c kardiografi impedancyjn (Niccomo, Medis, Niemcy). Do konstruowania z o onych wykresów Poincare, a nast pnie ich dekompozycji do prostszych wykresów mini-Poincare powsta ych ze zbiorów kolejnych 300 uderzeń serca wykorzystano w asne oprogramowanie. Wyniki: Chwilowe warto ci parametrów hemodynamicznych mie ci y si wza kresie 54-115 ml dla SV, 2,6-9,2 l/min dla CO, 880-2050 dyn s/cm5 dla SVR oraz 600-1300 ms dla odst pów RR. Za pomoc programu do dekompozycji dla wszystkich analizowanych parametrów oceniano ich tachogramy, z o one wykresy Poincare oraz wykresy zale no ci SD2-SD1. Wykres Poincare dla SV przyj kszta t zbli ony do ko a, dla CO i SVR elipsy z ogonami, natomiast dla odst pów RR kszta t najbardziej z o ony. Trajektorie ruchu punktów w przestrzeni SD2-SD1 uk ada y si niemal wzd u linii równowagi (przek tnej) mi dzy SD2 i SDl dla SV, CO i SVR. W przypadku odst pów RR w ród sko nych trajektorii wyró nia y si równie niemal pionowe tory ruchów, wiadcz ce o tym, e niewielkim przyrostom lub spadkom zmienno ci krótkoterminowej (SD1) towarzysz ogromne zmiany w zmienno ci d ugoterminowej (SD
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