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Search Results: 1 - 10 of 8981 matches for " Alejandro Aballay "
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Role of the Nervous System in the Control of Proteostasis during Innate Immune Activation: Insights from C. elegans
Alejandro Aballay
PLOS Pathogens , 2013, DOI: 10.1371/journal.ppat.1003433
Abstract:
C. elegans Germline-Deficient Mutants Respond to Pathogen Infection Using Shared and Distinct Mechanisms
Michael TeKippe,Alejandro Aballay
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0011777
Abstract: Reproduction extracts a cost in resources that organisms are then unable to utilize to deal with a multitude of environmental stressors. In the nematode C. elegans, development of the germline shortens the lifespan of the animal and increases its susceptibility to microbial pathogens. Prior studies have demonstrated germline-deficient nematodes to have increased resistance to Gram negative bacteria. We show that germline-deficient strains display increased resistance across a broad range of pathogens including Gram positive and Gram negative bacteria, and the fungal pathogen Cryptococcus neoformans. Furthermore, we show that the FOXO transcription factor DAF-16, which regulates longevity and immunity in C. elegans, appears to be crucial for maintaining longevity in both wild-type and germline-deficient backgrounds. Our studies indicate that germline-deficient mutants glp-1 and glp-4 respond to pathogen infection using common and different mechanisms that involve the activation of DAF-16.
Recovery from an Acute Infection in C. elegans Requires the GATA Transcription Factor ELT-2
Brian Head,Alejandro Aballay
PLOS Genetics , 2014, DOI: doi/10.1371/journal.pgen.1004609
Abstract: The mechanisms involved in the recognition of microbial pathogens and activation of the immune system have been extensively studied. However, the mechanisms involved in the recovery phase of an infection are incompletely characterized at both the cellular and physiological levels. Here, we establish a Caenorhabditis elegans-Salmonella enterica model of acute infection and antibiotic treatment for studying biological changes during the resolution phase of an infection. Using whole genome expression profiles of acutely infected animals, we found that genes that are markers of innate immunity are down-regulated upon recovery, while genes involved in xenobiotic detoxification, redox regulation, and cellular homeostasis are up-regulated. In silico analyses demonstrated that genes altered during recovery from infection were transcriptionally regulated by conserved transcription factors, including GATA/ELT-2, FOXO/DAF-16, and Nrf/SKN-1. Finally, we found that recovery from an acute bacterial infection is dependent on ELT-2 activity.
High-Throughput Isolation and Mapping of C. elegans Mutants Susceptible to Pathogen Infection
Laura E. Fuhrman, Kevin V. Shianna, Alejandro Aballay
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0002882
Abstract: We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor? as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5–8 megabase sub-chromosomal region.
GATA Transcription Factor Required for Immunity to Bacterial and Fungal Pathogens
Samantha Kerry, Michael TeKippe, Nathan C. Gaddis, Alejandro Aballay
PLOS ONE , 2006, DOI: 10.1371/journal.pone.0000077
Abstract: In the past decade, Caenorhabditis elegans has been used to dissect several genetic pathways involved in immunity; however, little is known about transcription factors that regulate the expression of immune effectors. C. elegans does not appear to have a functional homolog of the key immune transcription factor NF-κB. Here we show that that the intestinal GATA transcription factor ELT-2 is required for both immunity to Salmonella enterica and expression of a C-type lectin gene, clec-67, which is expressed in the intestinal cells and is a good marker of S. enterica infection. We also found that ELT-2 is required for immunity to Pseudomonas aeruginosa, Enterococcus faecalis, and Cryptococcus neoformans. Lack of immune inhibition by DAF-2, which negatively regulates the FOXO transcription factor DAF-16, rescues the hypersusceptibility to pathogens phenotype of elt-2(RNAi) animals. Our results indicate that ELT-2 is part of a multi-pathogen defense pathway that regulates innate immunity independently of the DAF-2/DAF-16 signaling pathway.
Nucleolar Proteins Suppress Caenorhabditis elegans Innate Immunity by Inhibiting p53/CEP-1
Laura E. Fuhrman,Ajay Kumar Goel,Jason Smith,Kevin V. Shianna,Alejandro Aballay
PLOS Genetics , 2009, DOI: 10.1371/journal.pgen.1000657
Abstract: The tumor suppressor p53 has been implicated in multiple functions that play key roles in health and disease, including ribosome biogenesis, control of aging, and cell cycle regulation. A genetic screen for negative regulators of innate immunity in Caenorhabditis elegans led to the identification of a mutation in NOL-6, a nucleolar RNA-associated protein (NRAP), which is involved in ribosome biogenesis and conserved across eukaryotic organisms. Mutation or silencing of NOL-6 and other nucleolar proteins results in an enhanced resistance to bacterial infections. A full-genome microarray analysis on animals with altered immune function due to mutation in nol-6 shows increased transcriptional levels of genes regulated by a p53 homologue, CEP-1. Further studies indicate that the activation of innate immunity by inhibition of nucleolar proteins requires p53/CEP-1 and its transcriptional target SYM-1. Since nucleoli and p53/CEP-1 are conserved, our results reveal an ancient immune mechanism by which the nucleolus may regulate immune responses against bacterial pathogens.
Tolerance of some Grapevine Rootstocks to Tylenchulus Semipenetrans Cobb 1914 in Chile
Aballay,Erwin; Navarro,Alejandra;
Agricultura Técnica , 2005, DOI: 10.4067/S0365-28072005000300010
Abstract: grapevine (vitis vinifera l.) rootstock resistance to tylenchulus semipenetrans cobb 1914 was tested in a field trial carried out in a pre-established grapevine crop. tested rootstocks included vr o39-16, 420 a, kober 5bb, 110 richter, harmony, couderc 1613, ramsey and so4. cultivar cabernet sauvignon was used as a control. a soil analysis was made at planting in 1995, and a second soil and root analysis was made in 2000 to assess the presence of juveniles and adult females. the most resistant rootstocks were ramsey and 110 richter; the others were similar to the control.
Nematicide Effect of Various Organic Soil Amendments on Meloidogyne ethiopica Whitehead, 1968, on Potted Vine Plants
Rivera,Lucia; Aballay,Erwin;
Chilean journal of agricultural research , 2008, DOI: 10.4067/S0718-58392008000300009
Abstract: five organic soil amendments were evaluated for their nematicide effect on root-knot meloidogyne ethiopica whitehead, 1968, on potted vitis vinifera l. var. chardonnay plants. the amendments included two immature composts: compost a made with tea (camellia sinensis (l.) kuntze) residues, broiler litter and grape pomace; compost b made with tea residues, rachis and grape pomace; and separately dried tea residues, grape pomace and broiler litter. these amendments were either incorporated or applied as cover to the substrate in 5 l pots at the beginning of spring. a chemical treatment with the nematicide fenamiphos and a control with substrate only were added for comparisons. the assay was composed of 11 treatments with six replicates, with a grape plant as experimental unit. seven months later, the assessment of the treatments was carried out based on number of second stage juvenile, nodules, eggs per root gram and reproductive index. fresh aerial and root plant weight were also measured. the chemical treatment presented the lowest final population of m. ethiopica. when evaluating the nematicide action of the organic amendments: compost a soil-incorporated and both grape pomace and solid dry tea residue as covers obtained low reproductive indexes, similar to the chemical treatment and control (p ≤ 0.05). no differences in aerial fresh weight were determined. however, the chemical treatment showed the smallest root mass as compared to compost b and solid dry tea residues, both as cover.
Tolerance of some Grapevine Rootstocks to Tylenchulus Semipenetrans Cobb 1914 in Chile Tolerancia de Algunos Portainjertos a Tylenchulus Semipenetrans Cobb 1914 en Chile
Erwin Aballay,Alejandra Navarro
Agricultura Técnica , 2005,
Abstract: Grapevine (Vitis vinifera L.) rootstock resistance to Tylenchulus semipenetrans Cobb 1914 was tested in a field trial carried out in a pre-established grapevine crop. Tested rootstocks included VR O39-16, 420 A, Kober 5BB, 110 Richter, Harmony, Couderc 1613, Ramsey and SO4. Cultivar Cabernet Sauvignon was used as a control. A soil analysis was made at planting in 1995, and a second soil and root analysis was made in 2000 to assess the presence of juveniles and adult females. The most resistant rootstocks were Ramsey and 110 Richter; the others were similar to the control. La resistencia de algunos portainjertos de vid (Vitis vinifera L.) a Tylenchulus semipenetrans Cobb 1914 fue evaluada en un ensayo desarrollado en un predio donde existió una vi a establecida en ese sector. Los portainjertos utilizados fueron VR O39-16, 420 A, Kober 5BB, 110 Richter, Harmony, Courdec 1613, Ramsey y SO4. La variedad Cabernet Sauvignon se usó como control. Se realizó un muestreo y análisis de suelos al momento de la plantación en 1995 y un segundo muestreo de suelos y raíces el a o 2000 para determinar la presencia de juveniles y hembras adultas. Los portainjertos más resistentes fueron Ramsey y 110 Richter; los otros se comportaron en forma similar al testigo.
Nematicide Effect of Various Organic Soil Amendments on Meloidogyne ethiopica Whitehead, 1968, on Potted Vine Plants Efecto Nematicida de Varias Enmiendas Orgánicas Sobre Meloidogyne ethiopica Whitehead, 1968, en Plantas de Vid en Macetas
Lucia Rivera,Erwin Aballay
Chilean Journal of Agricultural Research , 2008,
Abstract: Five organic soil amendments were evaluated for their nematicide effect on root-knot Meloidogyne ethiopica Whitehead, 1968, on potted Vitis vinifera L. var. Chardonnay plants. The amendments included two immature composts: Compost A made with tea (Camellia sinensis (L.) Kuntze) residues, broiler litter and grape pomace; Compost B made with tea residues, rachis and grape pomace; and separately dried tea residues, grape pomace and broiler litter. These amendments were either incorporated or applied as cover to the substrate in 5 L pots at the beginning of spring. A chemical treatment with the nematicide fenamiphos and a control with substrate only were added for comparisons. The assay was composed of 11 treatments with six replicates, with a grape plant as experimental unit. Seven months later, the assessment of the treatments was carried out based on number of second stage juvenile, nodules, eggs per root gram and reproductive index. Fresh aerial and root plant weight were also measured. The chemical treatment presented the lowest final population of M. ethiopica. When evaluating the nematicide action of the organic amendments: compost A soil-incorporated and both grape pomace and solid dry tea residue as covers obtained low reproductive indexes, similar to the chemical treatment and control (P ≤ 0.05). No differences in aerial fresh weight were determined. However, the chemical treatment showed the smallest root mass as compared to compost B and solid dry tea residues, both as cover. Se realizó un experimento en macetas con plantas enraizadas de Vitis vinífera L. var. Chardonnay para evaluar el efecto nematicida de diferentes enmiendas orgánicas sobre el nematodo agallador Meloidogyne ethiopica Whitehead, 1968. Se usaron dos composts inmaduros: Compost A, elaborado con residuos de té (Camellia sinensis (L.) Kuntze, guano de pollo seco y orujo de uva; Compost B, elaborado con residuos de té, escobajo y orujo de uva; y separadamente residuos sólidos de té; orujo de uva y guano de pollo seco. Estas enmiendas fueron aplicadas a principio de primavera, tanto en cobertera como incorporadas al suelo, considerando además un testigo químico fenamiphos y un testigo absoluto. Se determinó la población de nematodos de segundo estado juvenil (estado infestivo) de Meloidogyne spp., la cantidad de nódulos, huevos por gramo de raíz y el índice reproductivo, determinando de esta manera la tasa de reproducción de los nematodos. Además se evaluó el peso fresco de la parte aérea y de la masa radical. El testigo químico fue el tratamiento que obtuvo menor población final de
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