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Search Results: 1 - 10 of 1716 matches for " Alegria Carrasco-Pancorbo "
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Phenolic Molecules in Virgin Olive Oils: a Survey of Their Sensory Properties, Health Effects, Antioxidant Activity and Analytical Methods. An Overview of the Last Decade Alessandra
Alessandra Bendini,Lorenzo Cerretani,Alegria Carrasco-Pancorbo,Ana Maria Gómez-Caravaca,Antonio Segura-Carretero,Alberto Fernández-Gutiérrez,Giovanni Lercker
Molecules , 2007, DOI: 10.3390/12081679
Abstract: Among vegetable oils, virgin olive oil (VOO) has nutritional and sensory characteristics that to make it unique and a basic component of the Mediterranean diet. The importance of VOO is mainly attributed both to its high content of oleic acid a balanced contribution quantity of polyunsaturated fatty acids and its richness in phenolic compounds, which act as natural antioxidants and may contribute to the prevention of several human diseases. The polar phenolic compounds of VOO belong to different classes: phenolic acids, phenyl ethyl alcohols, hydroxy-isochromans, flavonoids, lignans and secoiridoids. This latter family of compounds is characteristic of Oleaceae plants and secoiridoids are the main compounds of the phenolic fraction. Many agronomical and technological factors can affect the presence of phenols in VOO. Its shelf life is higher than other vegetable oils, mainly due to the presence of phenolic molecules having a catechol group, such as hydroxytyrosol and its secoiridoid derivatives. Several assays have been used to establish the antioxidant activity of these isolated phenolic compounds. Typical sensory gustative properties of VOO, such as bitterness and pungency, have been attributed to secoiridoid molecules. Considering the importance of the phenolic fraction of VOO, high performance analytical methods have been developed to characterize its complex phenolic pattern. The aim of this review is to realize a survey on phenolic compounds of virgin olive oils bearing in mind their chemical-analytical, healthy and sensory aspects. In particular, starting from the basic studies, the results of researches developed in the last ten years will be focused.
Olive oil's bitter principle reverses acquired autoresistance to trastuzumab (Herceptin?) in HER2-overexpressing breast cancer cells
Javier A Menendez, Alejandro Vazquez-Martin, Ramon Colomer, Joan Brunet, Alegria Carrasco-Pancorbo, Rocio Garcia-Villalba, Alberto Fernandez-Gutierrez, Antonio Segura-Carretero
BMC Cancer , 2007, DOI: 10.1186/1471-2407-7-80
Abstract: Semi-preparative HPLC was used to isolate EVOO polyphenols (i.e., tyrosol, hydroxytyrosol, oleuropein). Both the anti-proliferative and the pro-apoptotic effects of EVOO phenolics were evaluated by using MTT-based quantification of metabolically viable cells and ELISA-based detection of histone-associated DNA fragments, respectively. The nature of the interaction between oleuropein aglycone and the anti-HER2 monoclonal antibody trastuzumab (Herceptin?) was mathematically evaluated by the dose-oriented isobologram technique. HER2-specific ELISAs were employed to quantitatively assess both the basal cleavage of the HER2 extracellular domain (ECD) and the expression level of total HER2. The activation status of HER2 was evaluated by immunoblotting procedures using a monoclonal antibody specifically recognizing the tyrosine phosphorylated (Phosphor-Tyr1248) form of HER2.Among EVOO polyphenols tested, oleuropein aglycone was the most potent EVOO phenolic in decreasing breast cancer cell viability. HER2 gene-amplified SKBR3 cells were ~5-times more sensitive to oleuropein aglycone than HER2-negative MCF-7 cells. Retroviral infection of the HER2 oncogene in MCF-7 cells resulted in a "SKBR3-assimilated" phenotype of hypersensitivity to oleuropein aglycone. An up to 50-fold increase in the efficacy of trastuzumab occurred in the presence of oleuropein aglycone. A preclinical model of acquired autoresistance to trastuzumab (SKBR3/Tzb100 cells) completely recovered trastuzumab sensitivity (> 1,000-fold sensitization) when co-cultured in the presence of oleuropein aglycone. Indeed, the nature of the interaction between oleuropein aglycone and trastuzumab was found to be strongly synergistic in Tzb-resistant SKBR3/Tzb100 cells. Mechanistically, oleuropein aglycone treatment significantly reduced HER2 ECD cleavage and subsequent HER2 auto-phosphorylation, while it dramatically enhanced Tzb-induced down-regulation of HER2 expression.Olive oil's bitter principle (i.e., oleuropein a
tabAnti-HER2 (erbB-2) oncogene effects of phenolic compounds directly isolated from commercial Extra-Virgin Olive Oil (EVOO)
Javier A Menendez, Alejandro Vazquez-Martin, Rocio Garcia-Villalba, Alegria Carrasco-Pancorbo, Cristina Oliveras-Ferraros, Alberto Fernandez-Gutierrez, Antonio Segura-Carretero
BMC Cancer , 2008, DOI: 10.1186/1471-2407-8-377
Abstract: Solid phase extraction followed by semi-preparative high-performance liquid chromatography (HPLC) was used to isolate phenolic fractions from commercial EVOO. Analytical capillary electrophoresis coupled to mass spectrometry was performed to check for the composition and to confirm the identity of the isolated fractions. EVOO polyphenolic fractions were tested on their tumoricidal ability against HER2-negative and HER2-positive breast cancer in vitro models using MTT, crystal violet staining, and Cell Death ELISA assays. The effects of EVOO polyphenolic fractions on the expression and activation status of HER2 oncoprotein were evaluated using HER2-specific ELISAs and immunoblotting procedures, respectively.Among the fractions mainly containing the single phenols hydroxytyrosol and tyrosol, the polyphenol acid elenolic acid, the lignans (+)-pinoresinol and 1-(+)-acetoxypinoresinol, and the secoiridoids deacetoxy oleuropein aglycone, ligstroside aglycone, and oleuropein aglycone, all the major EVOO polyphenols (i.e. secoiridoids and lignans) were found to induce strong tumoricidal effects within a micromolar range by selectively triggering high levels of apoptotic cell death in HER2-overexpressors. Small interfering RNA-induced depletion of HER2 protein and lapatinib-induced blockade of HER2 tyrosine kinase activity both significantly prevented EVOO polyphenols-induced cytotoxicity. EVOO polyphenols drastically depleted HER2 protein and reduced HER2 tyrosine autophosphorylation in a dose- and time-dependent manner. EVOO polyphenols-induced HER2 downregulation occurred regardless the molecular mechanism contributing to HER2 overexpression (i.e. naturally by gene amplification and ectopically driven by a viral promoter). Pre-treatment with the proteasome inhibitor MG132 prevented EVOO polyphenols-induced HER2 depletion.The ability of EVOO-derived polyphenols to inhibit HER2 activity by promoting the proteasomal degradation of the HER2 protein itself, together with the f
Algunas Estrategias Utilizadas por Familias Peruanas para Afrontar la Crisis Económica Actual
Alegria Majluf
Revista de Psicología , 1994,
Abstract: Con el fin de explorar las estrategias utilizadas por madres de CSE media y baja para afrontar la crisis económica actual se entrevistaron a 20 madres de cada estrato económico y se les administraron tres peque as encuestas y la Escala F-Copes "Escala de Evaluación Personal del Funcionamiento Familiar en Situaciones de Crisis". Los resultados evidenciaron que ambos grupos económicos se vieron seriamente afectados económicamente debiendo reducir sus gastos en actividades virales tales como alimentación, vestuario y recreación. Las madres de CSE media recurrieron más a una estrategia de reestructuración del problema para hacerlo más manejable y las madres de CSE baja a la movilización familiar para obtener ayuda y a la evaluación pasiva. No difieren ambos grupos en la búsqueda de apoyo espiritual y social.
Geografias do Mundo Imaginado
Alegria,Maria Fernanda;
Finisterra - Revista Portuguesa de Geografia , 2010,
Abstract: the idea that geography is more about the construction of images of the world than about a given ?objective reality? inspired this study of the mental representations of several countries. among the multiple possible representations of 12 countries (portugal, france, norway, egypt, mauritania, mozambique, united states, mexico, brazil, china, pakistan and saudi arabia), we began by charting their location in a world map as indicated by over 300 respondents with at least high school attendance. then, the compilation of a vast number of mental images (three per country and respondent) led to the definition of six general categories that bring together mental images of a similar kind: nature, culture and history, tourism and gastronomy, economy and society, specific places and no answer/wrong answers. in each category, we have highlighted the dominant (most frequent) idea. location, the relative frequency of the no answer/wrong answers category and the dominant images for each country all proved significant when it comes to dividing the countries into groups, and serve as indicators of the richness and variety of the mental representations. the study provided answers to some of the original research questions, but leaves many others unanswered.
Representa es sobre a imagem na aprendizagem geográfica
Maria Fernanda Alegria
Finisterra - Revista Portuguesa de Geografia , 2005,
Abstract: IMAGE REPRESENTATION IN GEOGRAPHICAL LEARNING. The word image can convey countless different meanings. Of the author-work-audience trilogy, the study of the two former has traditionally been the object of the greatest attention, and only recently has the focus started to shift to the reception of the visual messages as well. Every message is liable to being interpreted in a variety of different ways, but this polysemic characteristic is especially important in the case of images. Every image is an analogy, a representation, of reality – one so natural that reality and its representation are often mistaken for one another. In order to avoid this erroneous superposition, it is necessary to move beyond recognition and into interpretation. Several factors are involved in the latter: some easily discernible, others considerably more obscure. These include the habit of looking at images, the associations evoked by the elements present in the image, the expectations of the recipient, the degree of resistance to analysis and the presence/absence of additional comments. In conclusion, an account is rendered of carrying out an analysis of two cartoons with a strong geographical content with 9th and 11th grade students. Thisis used to draw a number of conclusions and suggest ideas for urther research.
As recentes altera es no currículo obrigatório de Geografia em Portugal (1989-2001)
Maria Fernanda Alegria
Finisterra - Revista Portuguesa de Geografia , 2002,
Abstract: RECENT CHANGES IN COMPULSORY SCHOOL EDUCATION CURRICULUM OF GEOGRAPHY IN PORTUGAL (1989-2001). The two recent reforms in compulsory school education in Portugal (decrees 286/89 and 6/2001) have affected geography teaching. Changes made between 1989 and 2001 included: setting up 90-minute classes with less geography teaching on a weekly basis; organising timetables with history classes; teaching geography at the 7th, 8th and 9th grade level since 2001,whereas in 1989 geography classes ceased at 8th grade. Curriculum contents were changed unlike what had been initially planned. The first part of the paper deals with the idea of ‘competence’, central to more recent educational reform, with a summary of the meaning of the three new so-called transversal curriculum items (civic training, project planning and structured learning), as well as with an understanding of the dissemination of educational reform throughout the country and the advantages and difficulties of schools being more autonomous in their curriculum organisation. The second part explores the usefulness of geography in compulsory education, the justification for some resources and an assessment ofthe qualities and difficulties of the 2001 reform.
Os mapas na história da descoberta do Novo Mundo
Maria Fernanda Alegria
Finisterra - Revista Portuguesa de Geografia , 1994,
Abstract:
Geografias do mundo imaginário
Maria Fernanda Alegria
Finisterra - Revista Portuguesa de Geografia , 2010,
Abstract: Geographies of the imagined world. The idea that Geography ismore about the construction of images of the world than about a given “objective reality” inspired this study of the mental representations of several countries. Among the multiple possible representations of 12 countries (Portugal, France, Norway, Egypt,Mauritania, Mozambique, United States, Mexico, Brazil, China, Pakistan and Saudi Arabia), we began by charting their location in a world map as indicated by over 300 respondents with at least high school attendance. Then, the compilation of a vast number of mental images (three per country and respondent) led to the definition ofsix general categories that bring together mental images of a similar kind: nature, culture and history, tourism and gastronomy, economy and society, specific places and no answer/wrong answers. In each category, we have highlighted the dominant (most frequent)idea. Location, the relative frequency of the no answer/wrong answerscategory and the dominant images for each country all proved significant when it comes to dividing the countries into groups, and serve as indicators of the richness and variety of the mental representations. The study provided answers to some of the original research questions, but leaves many others unanswered.
O desenvolvimento da indústria na regi o de Coimbra entre 1867 e 1927
Maria Fernanda Alegria
Finisterra - Revista Portuguesa de Geografia , 1986,
Abstract:
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