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Search Results: 1 - 10 of 228 matches for " Aldina Barral "
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Interleukin- 2 production during murine infection by Leishmania mexicana amazonensis
Barral-Netto, Manoel;Roters, Silene B.;Barral, Aldina;
Memórias do Instituto Oswaldo Cruz , 1986, DOI: 10.1590/S0074-02761986000100005
Abstract: highly susceptible balb/c mice, resistant c57b1/6 and their f1 progeny (bdf1) were infected subcutaneously in the foot pad with leishmania mexicana amazonenesis. at various times after infection, spleen or draining popliteal lymph node cells were assayed for their capacity to generate interleukin-2 (i1-2) by concanavalin a (cona) stimulation. in both balb/c and c57b1/6 strains there was a transient increase in their capacity to produce i1-2, from the 3rd to the 10th week post-infection. return to pre-infection levels ocurred between 13th to 16th week post-infection in all three strains. balb/c mice always produced higher titers of 11-2 than c57b1/6, but such differences were statistically significant only at 3 and 10 weeks post-infection. bdf1 mice had titers similar to those observed in balb/c mice. i1-2 production by cona-stimulated lymph node cells was lower as compared to the spleen, but with a similar pattern among the three mice strains. our data show that susceptibility to infection by l. mexicana amazonenesis is not associated with deficient cona-stimulated i1-2 production.
Haematophagous arthropod saliva and host defense system: a tale of tear and blood
Andrade, Bruno B.;Teixeira, Clarissa R.;Barral, Aldina;Barral-Netto, Manoel;
Anais da Academia Brasileira de Ciências , 2005, DOI: 10.1590/S0001-37652005000400008
Abstract: the saliva from blood-feeding arthropod vectors is enriched with molecules that display diverse functions that mediate a successful blood meal. they function not only as weapons against host's haemostatic, inflammatory and immune responses but also as important tools to pathogen establishment. parasites, virus and bacteria taking advantage of vectors' armament have adapted to facilitate their entry in the host. today, many salivary molecules have been identified and characterized as new targets to the development of future vaccines. here we focus on current information on vector's saliva and the molecules responsible to modify host's hemostasis and immune response, also regarding their role in disease transmission.
A simple method for human peripheral blood monocyte Isolation
Almeida, Marcos C de;Silva, Alan C;Barral, Aldina;Barral Netto, Manoel;
Memórias do Instituto Oswaldo Cruz , 2000, DOI: 10.1590/S0074-02762000000200014
Abstract: we describe a simple method using percoll gradient for isolation of highly enriched human monocytes. high numbers of fully functional cells are obtained from whole blood or buffy coat cells. the use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.
Abstract
Barral Aldina,Carvalho Edgar,Barral-Netto Manoel,De Souza Wanderley
Memórias do Instituto Oswaldo Cruz , 1998,
Abstract:
A simple method for human peripheral blood monocyte Isolation
Almeida Marcos C de,Silva Alan C,Barral Aldina,Barral Netto Manoel
Memórias do Instituto Oswaldo Cruz , 2000,
Abstract: We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.
Haematophagous arthropod saliva and host defense system: a tale of tear and blood
Andrade Bruno B.,Teixeira Clarissa R.,Barral Aldina,Barral-Netto Manoel
Anais da Academia Brasileira de Ciências , 2005,
Abstract: The saliva from blood-feeding arthropod vectors is enriched with molecules that display diverse functions that mediate a successful blood meal. They function not only as weapons against host's haemostatic, inflammatory and immune responses but also as important tools to pathogen establishment. Parasites, virus and bacteria taking advantage of vectors' armament have adapted to facilitate their entry in the host. Today, many salivary molecules have been identified and characterized as new targets to the development of future vaccines. Here we focus on current information on vector's saliva and the molecules responsible to modify host's hemostasis and immune response, also regarding their role in disease transmission.
Distinct ultrastructural aspects in different biopsies of a single patient with diffuse cutaneous leishmaniasis
Bittencourt, Achilea Lisboa;Freitas, Luiz Antonio Rodrigues de;Pompeu, Margarida L.;Vieira, Maria Lucia;Barral, Aldina;
Memórias do Instituto Oswaldo Cruz , 1990, DOI: 10.1590/S0074-02761990000100008
Abstract: the authors investigated the relation between parasites and host-cells in active and regressed lesions of a patient with diffuse cutaneous leishmaniasis, evaluating the frequency of different cell types, and the location and integrity of amastigotes. no correlation was found between parasite integrity and size of parasitophorous vacuoles. they observed ultrastructural findings characterizing a cell mediated immune response: macrophages lysis, parasitic destruction inside macrophages, close contact between parasitized macrophages and lymphocytes and between parasites and lymphocytes, lymphocytic infiltration and fibrosis. they suggest that in dcl there is a limited cellular immune response, although insufficient to control infection.
New Insights on the Inflammatory Role of Lutzomyia longipalpis Saliva in Leishmaniasis
Deboraci Brito Prates,Théo Araújo-Santos,Cláudia Brodskyn,Manoel Barral-Netto,Aldina Barral,Valéria Matos Borges
Journal of Parasitology Research , 2012, DOI: 10.1155/2012/643029
Abstract: When an haematophagous sand fly vector insect bites a vertebrate host, it introduces its mouthparts into the skin and lacerates blood vessels, forming a hemorrhagic pool which constitutes an intricate environment of cell interactions. In this scenario, the initial performance of host, parasite, and vector “authors” will heavily influence the course of Leishmania infection. Recent advances in vector-parasite-host interaction have elucidated “co-authors” and “new roles” not yet described. We review here the stimulatory role of Lutzomyia longipalpis saliva leading to inflammation and try to connect them in an early context of Leishmania infection. 1. Introduction Leishmaniasis remains a serious problem in public health, endemic in 88 countries on four continents, but most of the cases occur in underdeveloped or developing countries [1]. Visceral Leishmaniasis (VL) is a progressive infection with fatal outcome in the absence of treatment. Approximately 90% of the VL cases registered in the Americas occur in Brazil and are concentrated in the Northeast region. In the New World, Lutzomyia longipalpis is the principal vector of Leishmania infantum chagasi, the agent of American Visceral Leishmaniasis [2]. The causes related to development of distinct clinical manifestations in leishmaniasis are multifactorial and reflect the complexity at the vector-pathogen-host interface [3]. Protozoan parasites of the genus Leishmania are the causative agents of the disease and are transmitted to the mammalian hosts by the bite of female phlebotomine sand flies during blood repast. For blood meal obtainment, sand flies introduce their mouthparts into the skin, tearing tissues, lacerating capillaries, and creating haemorrhagic pools upon which they feed [4]. The presence of sand fly saliva in the blood pool, the environment where the parasite encounters host cells, influences the development and functions of several leukocytes. In recent years, the importance of the interaction between components of sand fly saliva and host immune mechanisms in regulating infectivity and disease progression has become clearer and suggests their consequences to disease outcome in leishmaniasis [5]. The aspects involved in immune response resulting in resistance or susceptibility widely depend on the first attempt of host’s innate response to contain infection that may influence on the predominance of a pattern of future host’s immune adaptive response against Leishmania. Many studies have been performed to understand the mechanisms leading to protection or exacerbation of the disease however;
Plasma Superoxide Dismutase-1 as a Surrogate Marker of Vivax Malaria Severity
Bruno B. Andrade,Antonio Reis-Filho,Sebasti?o Martins Souza-Neto,Imbroinise Raffaele-Netto,Luis M. A. Camargo,Aldina Barral,Manoel Barral-Netto
PLOS Neglected Tropical Diseases , 2010, DOI: 10.1371/journal.pntd.0000650
Abstract: Background Severe outcomes have been described for both Plasmodium falciparum and P. vivax infections. The identification of sensitive and reliable markers of disease severity is fundamental to improving patient care. An intense pro-inflammatory response with oxidative stress and production of reactive oxygen species is present in malaria. Inflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and antioxidant agents such as superoxide dismutase-1 (SOD-1) are likely candidate biomarkers for disease severity. Here we tested whether plasma levels of SOD-1 could serve as a biomarker of severe vivax malaria. Methodology/Principal Findings Plasma samples were obtained from residents of the Brazilian Amazon with a high risk for P. vivax transmission. Malaria diagnosis was made by both microscopy and nested PCR. A total of 219 individuals were enrolled: non-infected volunteers (n = 90) and individuals with vivax malaria: asymptomatic (n = 60), mild (n = 50) and severe infection (n = 19). SOD-1 was directly associated with parasitaemia, plasma creatinine and alanine amino-transaminase levels, while TNF-alpha correlated only with the later enzyme. The predictive power of SOD-1 and TNF-alpha levels was compared. SOD-1 protein levels were more effective at predicting vivax malaria severity than TNF-alpha. For discrimination of mild infection, elevated SOD-1 levels showed greater sensitivity than TNF-alpha (76% vs. 30% respectively; p<0.0001), with higher specificity (100% vs. 97%; p<0.0001). In predicting severe vivax malaria, SOD-1 levels exhibited higher sensitivity than TNF-alpha (80% vs. 56%, respectively; p<0.0001; likelihood ratio: 7.45 vs. 3.14; p<0.0001). Neither SOD-1 nor TNF-alpha could discriminate P. vivax infections from those caused by P. falciparum. Conclusion SOD-1 is a powerful predictor of disease severity in individuals with different clinical presentations of vivax malaria.
DETC Induces Leishmania Parasite Killing in Human In Vitro and Murine In Vivo Models: A Promising Therapeutic Alternative in Leishmaniasis
Ricardo Khouri,Fernanda Novais,Gisélia Santana,Camila Indiani de Oliveira,Marcos André Vannier dos Santos,Aldina Barral,Manoel Barral-Netto,Johan Van Weyenbergh
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0014394
Abstract: Chemotherapy remains the primary tool for treatment and control of human leishmaniasis. However, currently available drugs present serious problems regarding side-effects, variable efficacy, and cost. Affordable and less toxic drugs are urgently needed for leishmaniasis.
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