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Search Results: 1 - 10 of 143194 matches for " Ajoy K. Roy "
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Genetic Diversity and Lineage Based on SSR Markers of Two Genomic Resources among Trifolium Collections Held within the Australian Pastures Genebank  [PDF]
Devendra R. Malaviya, Harsh Raman, Brian S. Dear, Rosy Raman, Ajoy K. Roy, Pankaj Kaushal, Amaresh Chandra, Steve J. Hughes
Open Journal of Genetics (OJGen) , 2019, DOI: 10.4236/ojgen.2019.91001
Abstract: Trifolium alexandrinum, an important forage legume, suffers from narrow genetic base. The present investigation was envisaged to reveal the inter- and intra-species genetic diversity and lineage among 64 accessions, representing a global collection, of T. alexandrinum; it’s two probable progenitor species (T. salmoneum and T. subterraneum) and the three genetically distant species (T. repens, T. vesiculosum, T. michelianum). A set of Simple Sequence Repeats (SSR) primer-pairs developed from T. alexandrinum have shown to amplify alleles across the species under study, suggesting utility of the newly developed resource for assessing molecular diversity among Trifolium species. These SSRs markers together with previously reported SSRs, derived from T. repens, enabled to reveal high intra-species polymorphism in T. alexandrinum and successfully discriminate different species investigated in this study. The diverse accessions determined herein provide a superior resource for further breeding
Exploiting EST databases for the development and characterisation of 3425 gene-tagged CISP markers in biofuel crop sugarcane and their transferability in cereals and orphan tropical grasses
Amaresh Chandra, Radha Jain, Sushil Solomon, Shiksha Shrivastava, Ajoy K Roy
BMC Research Notes , 2013, DOI: 10.1186/1756-0500-6-47
Abstract: Findings: Utilising 246,180 Saccharum officinarum EST sequences vis-a-vis its comparative analysis with ESTs of sorghum and barley and the whole rice genome sequence, we have developed 3425 novel gene-tagged markers---namely, conserved-intron scanning primers (CISP)---using the web program GeMprospector. Rice orthologue annotation results indicated homology of 1096 sequences with expressed proteins, 491 with hypothetical proteins. The remaining 1838 were miscellaneous in nature. A total of 367 primer-pairs were tested in diverse panel of samples. The data indicate amplification of 41% polymorphic bands leading to 0.52 PIC and 3.50 MI with a set of sugarcane varieties and Saccharum species. In addition, a moderate technical functionality of a set of such markers with orphan tropical grasses (22%) and fodder cum cereal oat (33%) is observed.Developed gene-tagged CISP markers exhibited considerable technical functionality with varieties of sugarcane and unexplored species of tropical grasses. These markers would thus be particularly useful in identifying the economical traits in sugarcane and developing conservation strategies for orphan tropical grasses.
An evaluation of UV protection imparted by cotton fabrics dyed with natural colorants
Ajoy K Sarkar
BMC Dermatology , 2004, DOI: 10.1186/1471-5945-4-15
Abstract: Three cotton fabrics were dyed with three natural colorants. Fabrics were characterized with respect to fabric construction, weight, thickness and thread count. Influence of fabric characteristics on Ultraviolet Protection Factor was studied. Role of colorant concentration on the ultraviolet protection factor was examined via color strength analysis.A positive correlation was observed between the weight of the fabric and their UPF values. Similarly, thicker fabrics offered more protection from ultraviolet rays. Thread count appears to negatively correlate with UPF. Dyeing with natural colorants dramatically increased the protective abilities of all three fabric constructions. Additionally, within the same fabric type UPF values increased with higher depths of shade.Dyeing cotton fabrics with natural colorants increases the ultraviolet protective abilities of the fabrics and can be considered as an effective protection against ultraviolet rays. The UPF is further enhanced with colorant of dark hues and with high concentration of the colorant in the fabric.High, short-term exposure to ultraviolet radiation (UVR) from the sun causes sunburns and long-term exposure leads to skin cancer. The National Toxicology Program, U.S. Department of Health and Human Services has classified UVR as a known human carcinogen [1]. The American Cancer Society estimates that more than one million cases of skin cancer cases are diagnosed each year in the United States [2]. In 2002, an estimated 54,200 new cases of melanoma skin cancer alone were diagnosed [2]. A primary reason for the increased incidence of skin cancers is attributed to ozone depletion. Each one percent decrease in ozone concentration is predicted to increase the rate of skin cancer by two percent to five percent [3]. The United States Environmental Protection Agency estimates that ozone depletion will lead to between three and fifteen million new cases of skin cancer in the United States by the year 2075. Other reasons fo
Overexpression of Prothymosin Alpha Predicts Poor Disease Outcome in Head and Neck Cancer
Satyendra Chandra Tripathi,Ajay Matta,Jatinder Kaur,Jorg Grigull,Shyam Singh Chauhan,Alok Thakar,Nootan Kumar Shukla,Ritu Duggal,Ajoy Roy Choudhary,Siddhartha DattaGupta,Mehar Chand Sharma,Ranju Ralhan,K. W. Michael Siu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019213
Abstract: In our recent study, tissue proteomic analysis of oral pre-malignant lesions (OPLs) and normal oral mucosa led to the identification of a panel of biomarkers, including prothymosin alpha (PTMA), to distinguish OPLs from histologically normal oral tissues. This study aimed to determine the clinical significance of PTMA overexpression in oral squamous cell hyperplasia, dysplasia and head and neck squamous cell carcinoma (HNSCC).
Phytochemical Screening, Cytotoxicity and Antibacterial Activities of Two Bangladeshi Medicinal Plants
Ajoy Roy,Subrata Kumar Biswas,Anusua Chowdhury,Manik Chandra Shill
Pakistan Journal of Biological Sciences , 2011,
Abstract: The objectives of the present study were to investigate phytochemical screening and to assay cytotoxicity and antibacterial activities of ethanolic extracts of leaves of two medicinal plants, Aglaonema hookerianum Schott (Family: Araceae) and Lannea grandis Engl. (Family: Anacardiaceae) available in Bangladesh. The brine shrimp lethality bioassay showed that the ethanolic extracts of Aglaonema hookerianum and Lannea grandis possessed cytotoxic activities with LC50 5.25 (g mL-1) and 5.75 (g mL-1) and LC90 10.47 (g mL-1) and 9.55 (g mL-1), respectively. Two extracts obtained from leaves were examined for their antibacterial activities against some gram positive bacteria such as Bacillus subtilis, Bacillus megaterium and Staphylococcus aureus, also gram negative strains of Pseudomonas aeruginosa, Escherichia coli, Shigella dysenteriae, Salmonella typhi, Salmonella paratyphi and Vibrio cholerae. Agar disc diffusion method was applied to observe the antibacterial efficacy of the extracts. Results indicated that both plant extracts (500 g disc-1) displayed antibacterial activity against all of the tested microorganisms. These results were also compared with the zones of inhibition produced by commercially available standard antibiotic, Amoxicillin at concentration of 10 g disc-1. Observed antibacterial properties of the ethanolic extract of Aglaonema hookerianum Schott and Lannea grandis Engl. showed that both plants might be useful sources for the development of new potent antibacterial agents.
Umesh K. Dwivedi,Ajoy Ghosh,Navin Chand
BioResources , 2009,
Abstract: This experimental study was conducted to investigate the role of poly-vinyl-alcohol (PVA) treatment in improving the sliding wear behaviour of pure bamboo. The effects of dipping time in PVA solution and applied load on wear behaviour of bamboo samples were determined. The wear volume of bamboo was reduced when it was treated with PVA. The wear volume during sliding was increased with increasing of applied load, whereas the coefficient of friction was reduced on PVA treatment. Worn surfaces were observed by using SEM and discussed to explain the mechanism of wear.
Umesh K. Dwivedi,Ajoy Ghosh,Navin Chand
BioResources , 2007,
Abstract: An experimental study was conducted to determine the abrasive wear behaviour of different weight percentage bamboo powder filled polyester composites under the multipass mode. The effect of bamboo powder concentration and sliding distance on the weight loss of composites has been analyzed. Worn surface have been analyzed to observe the mechanism of wear. The weight loss depends on bamboo powder concentration. The weight loss decreases with the increase of sliding distance. Samples having 20 weight percentage (wt%) of bamboo powder show the maximum weight loss during abrasion.
Evaluation of Durability to Laundering of Triclosan and Chitosan on a Textile Substrate
Anupama Sargur Ranganath,Ajoy K. Sarkar
Journal of Textiles , 2014, DOI: 10.1155/2014/812303
Abstract: In a hospital environment, textile substrates have been implicated as a vector in the transmission of disease. To mitigate the harmful spread of disease via textile substrates, an effective measure is treatment of textiles with antimicrobial agents. The current investigation compares one of the most widely used chemically synthesized antimicrobial agents, triclosan with chitosan, a naturally occurring antimicrobial agent. For the study, samples of a common polyester/cotton textile used in hospital settings were treated with triclosan and chitosan based antimicrobial agents. Following treatment, the samples were analyzed for their effectiveness against Staphylococcus aureus and Escherichia coli using standard AATCC Test Methods. The efficacy of the treatment to laundering was then evaluated by subjecting the treated samples to 50 washings and repeating the tests against the challenge microorganisms. Data obtained were statistically analyzed at a 95% confidence interval. Results showed that before laundering both triclosan and chitosan treated samples were extremely effective as antimicrobial agents. After laundering, chitosan was less effective against E.coli but maintained efficacy against S. aureus. The effectiveness of triclosan was not adversely affected after the laundering treatments. 1. Introduction The Centers for Disease Control and Prevention, USA, estimate that approximately 1.7 million healthcare associated infections (HAIs) and 98,987 associated deaths occur each year on account of infection-causing bacteria. Hence, the control of infections has been identified as the most important target by the United States Department of Health and Human Services [1]. Textile substrates have been implicated as one of the vectors of transmission of infections. Fortuitously, the use of antimicrobial textiles is an effective deterrent against the spread of infection-causing bacteria [2]. Triclosan, a chlorinated phenolic compound, is a derivative of a diphenyl ether compound. Triclosan is most widely used in various medical and consumer care products. Triclosan belongs to leaching or controlled release category of antimicrobial agents [2, 3]. The mechanism of action of triclosan on a textile substrate is illustrated in Figure 1. The figure depicts a treated fiber containing a reservoir of antimicrobial agent. When the chemical agent ions are released outside the fiber they destroy microbes present in the vicinity. Triclosan inhibits the growth of microbes by using an electrochemical mode of action to penetrate and disrupt the cell wall of microbes [2, 4]. It
Depression in adult males with lower extremity amputation and its bio-social correlates  [PDF]
Arupendra Mozumdar, Subrata K. Roy
Health (Health) , 2010, DOI: 10.4236/health.2010.28131
Abstract: Depression is often associated with many adverse health conditions and lower socio-economic status. Stressful conditions like presence of disability coupled with traditional negative societal attitude towards disability may result in higher level of depression in the individuals with disability than in general population and can cause other health problems as well. Therefore, the purposes of this study are to explore the association of depression with its biosocial correlates among individuals with lower extremity amputations (LEA) living in Kolkata, India. Eighty-five participants with traumatic LEA and 105 control participants with no amputation participated in this study. The depression levels of the participants were measured with Beck Depression Index (BDI). All participants were also measured with the following sets of health traits, using standard techniques-1) physical: body weight, stature, body mass index (BMI), blood pressure, total cholesterol, triglycerides, blood glucose, hemoglobin%, 2) functional: functional outcome or degree of independence in daily activities, and 3) social: economic condition, and social discomfort. No significant difference was found in mean BDI scores for individuals with LEA and control participants. Higher BDI scores were associated with chronic energy deficiency. None of the other physical health traits was associated with depression level. Higher depression was associated with poor socio-economic conditions like low economic condition, dependency on others for daily activity, occupational constraints due to disability and perceived problems in conjugal life. Therefore, economic development with a positive social attitude toward people with disability is essential for the mental wellbeing of the individuals with LEA.
Ultrafast laser inscription: an enabling technology for astrophotonics
Robert R. Thomson,Ajoy K. Kar,Jeremy Allington-Smith
Physics , 2009, DOI: 10.1364/OE.17.001963
Abstract: The application of photonics to astronomy offers major advantages in the area of highly-multiplexed spectroscopy, especially when applied to extremely large telescopes. These include the suppression of the near-infrared night-sky spectrum [J. Bland-Hawthorn et al, Opt. Express 12, 5902 (2004), S. G. Leon-Saval et al, Opt. Lett. 30, 2545 (2005)] and the miniaturisation of spectrographs so that they may integrated into the light-path of individual spatial samples [J. Bland-Hawthorn et al, Proc SPIE 6269, 62690N (2006)]. Efficient collection of light from the telescope requires multimode optical fibres and three-dimensional photonic devices. We propose ultrafast laser inscription (ULI) [R. R. Thomson et al, Opt. Express 15, 11691 (2007)] as the best technology to fabricate 3D photonic devices for astrophotonic applications.
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