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Search Results: 1 - 10 of 80847 matches for " Aizhong Liu "
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Diagnosis of Premature Senescence of Cotton Using SPAD Value  [PDF]
Pengcheng Li, Helin Dong, Aizhong Liu, Jingran Liu, Miao Sun, Guoping Wang, Siping Zhang, Yabing Li, Shuchun Mao
Agricultural Sciences (AS) , 2014, DOI: 10.4236/as.2014.511107
Abstract: Field experiments were conducted in 2011 on an experimental farm at the Cotton Research Insti-tute, Chinese Academy of Agricultural Sciences, Anyang, China. We conducted experiments with a “SPAD-502” meter to quickly measure the relative value of chlorophyll content in the leaf blades of field cotton. Our goal was to seek a suitable leaf on a cotton plant to diagnose senescence status of crop plants at later stages of growth. We began by studying the dynamic change rule of the dis-tal-most four leaves of the cotton plant during the entire growth period with two early-maturing cultivars (CCRI 36, CCRI 50) and two mid-maturing cultivars (CCRI 41, SCRC 28). We also examined the effect of different nitrogen and potassium fertilizer rates on SPAD values of the leaves of SCRC 28. Our results suggest that SPAD values of the 1st distal stem leaves from two early cultivars can act as good indicators of senescence status in the plants, if they are measured between 10 d before the boll-opening stage and 10 d after boll opening stage. Differences of SPAD values of the 3rd distal stem leaves of two mid-maturing cultivars measured between about 15 d before the boll opening stage and 15 d after the boll opening stage can also be used to measure senescence status in these cotton cultivars. The conclusion can be used for cotton producer to manage N fertilizer better at later growth stage.
Prediction of posttraumatic stress disorder among adults in flood district
Peng Huang, Hongzhuan Tan, Aizhong Liu, Shuidong Feng, Mengshi Chen
BMC Public Health , 2010, DOI: 10.1186/1471-2458-10-207
Abstract: A cross-sectional survey was carried out in 2000 among individuals who were affected by the 1998 floods in Hunan, China. Multi-stage sampling was used to select subjects from the flood-affected areas. Data was collected through face-to-face interviews using a questionnaire. PTSD was diagnosed according to DSM-IV criteria. Study subjects were randomly divided into two groups: group 1 was used to establish the prediction model and group 2 was used to validate the model. We first used the logistic regression analysis to select predictive variables and then established a risk score predictive model. The validity of model was evaluated by using the model in group 2 and in all subjects. The area under the receiver operation characteristic (ROC) curve was calculated to evaluate the accuracy of the prediction model.A total of 2336 (9.2%) subjects were diagnosed as probable PTSD-positive individuals among a total of 25,478 study subjects. Seven independent predictive factors (age, gender, education, type of flood, severity of flood, flood experience, and the mental status before flood) were identified as key variables in a risk score model. The area under the ROC curve for the model was 0.853 in the validation data. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of this risk score model were 84.0%, 72.2%, 23.4%, and 97.8%, respectively, at a cut-off value of 67.5 in the validation data.A simple risk score model can be used to predict PTSD among victims of flood.Flood is one of the most common and severe forms of natural disasters. It can result in direct economic and property losses, physical injuries, deaths, and psychological injuries. Posttraumatic stress disorder (PTSD) is a common disorder among victims of various disasters such as traffic accidents[1,2], violent crimes[3], hurricanes[4], earthquakes[5,6], and floods [7-10]. PTSD is also a severe and complex disorder precipitated by exposure to psychologically distressi
Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages
Wang Xiaojuan,Xu Ronghua,Wang Ruling,Liu Aizhong
BMC Genomics , 2012, DOI: 10.1186/1471-2164-13-716
Abstract: Background Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3, a kind of ω-3 FAs). However, little is known about the molecular mechanisms responsible for biosynthesis of unsaturated fatty acids in the developing seeds of this species. Transcriptome data are needed to better understand these mechanisms. Results In this study, de novo transcriptome assembly and gene expression analysis were performed using Illumina sequencing technology. A total of 52.6 million 90-bp paired-end reads were generated from two libraries constructed at the initial stage and fast oil accumulation stage of seed development. These reads were assembled into 70,392 unigenes; 22,179 unigenes showed a 2-fold or greater expression difference between the two libraries. Using this data we identified unigenes that may be involved in de novo FA and triacylglycerol biosynthesis. In particular, a number of unigenes encoding desaturase for formation of unsaturated fatty acids with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified. Conclusions This study provides the first comprehensive dataset characterizing Sacha Inchi gene expression at the transcriptional level. These data provide the foundation for further studies on molecular mechanisms underlying oil accumulation and PUFA biosynthesis in Sacha Inchi seeds. Our analyses facilitate understanding of the molecular mechanisms responsible for the high unsaturated fatty acids (especially α-linolenic acid) accumulation in Sacha Inchi seeds.
Transcriptome-Wide Identification and Characterization of MicroRNAs from Castor Bean (Ricinus communis L.)
Wei Xu, Qinghua Cui, Fei Li, Aizhong Liu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0069995
Abstract: Background MicroRNAs (miRNAs) are endogenously encoded small RNAs that post-transcriptionally regulate gene expression and play essential roles in numerous developmental and physiological processes. Currently, little information on the transcriptome and tissue-specific expression of miRNAs is available in the model non-edible oilseed crop castor bean (Ricinus communis L.), one of the most important non-edible oilseed crops cultivated worldwide. Recent advances in sequencing technologies have allowed the identification of conserved and novel miRNAs in many plant species. Here, we used high-throughput sequencing technologies to identify and characterize the miRNAs in castor bean. Results Five small RNA libraries were constructed for deep sequencing from root tips, leaves, developing seeds (at the initial stage, seed1; and at the fast oil accumulation stage, seed2) and endosperms in castor bean. High-throughput sequencing generated a large number of sequence reads of small RNAs in this study. In total, 86 conserved miRNAs were identified, including 63 known and 23 newly identified. Sixteen miRNA isoform variants in length were found from the conserved miRNAs of castor bean. MiRNAs displayed diverse organ-specific expression levels among five libraries. Combined with criteria for miRNA annotation and a RT-PCR approach, 72 novel miRNAs and their potential precursors were annotated and 20 miRNAs newly identified were validated. In addition, new target candidates for miRNAs newly identified in this study were proposed. Conclusions The current study presents the first high-throughput small RNA sequencing study performed in castor bean to identify its miRNA population. It characterizes and increases the number of miRNAs and their isoforms identified in castor bean. The miRNA expression analysis provides a foundation for understanding castor bean miRNA organ-specific expression patterns. The present study offers an expanded picture of miRNAs for castor bean and other members in the family Euphorbiaceae.
Risks of Enterprise Merger & Purchase and the Prevention
Aizhong Chen
International Journal of Business and Management , 2009, DOI: 10.5539/ijbm.v3n6p111
Abstract: Enterprise merger & purchase is a complex systematic project. It concerns interests of many parts. In China, risks of enterprise merger & purchase include systematic risk, evaluating risk, financing risk, operational risk, corporate culture risk, and legal risk. These risks directly influence the merger & purchase activities. Therefore, we should deal with them carefully, adopt prevention measures, and guarantee the success of merger & purchase.
胸腔镜下 T4 交感神经干切断术对原发性手汗症患者生活质量的影响
Effect of T4 endoscopic thoracic sympathicotomy on life quality in patients with primary palmar hyperhidrosis

, LIU Aizhong, LIU Wenliang

- , 2015, DOI: 10.11817/j.issn.1672-7347.2015.10.012
Abstract: 目的:应用改良生活质量量表评价胸腔镜下T4交感神经干切断术前、术后原发性手汗症患者生活质量的 变化。方法:收集2009年6月至2014年5月接受胸腔镜下双侧T4交感神经干切断术治疗原发性手汗症患者48例。手术 前后患者完成生活质量自评、改良生活质量量表并予以量化评分。术后1,6个月进行随访并收集手术效果、并发 症、满意度、生活质量调查资料。结果:48例患者均顺利完成双侧同期胸腔镜下T4交感神经干切断术,无死亡及严 重并发症,未发生术中中转开胸情况。术后轻度代偿性多汗38例(79.1%),中度1例(2.1%),无重度代偿性多汗。47例 (97.9%)对手术效果非常满意,没有不满意以及后悔接受手术治疗病例。48例患者均顺利完成改良的手汗症专用生活 质量量表问卷,生活质量量表评测显示手汗症患者术后生活质量比手术前明显改善(F=763.67,P<0.001)。结论:原发 性手汗症严重影响患者的生活质量。胸腔镜下T4交感神经干切断术不仅明显减少手部出汗量,而且可以明显改善患 者生活质量
Effect of T4 thoracoscopic sympathectomy on plantar hyperhidrosis in patients with primary palmoplantar hyperhidrosis

, LIU Aizhong, LIU Wenliang

- , 2016, DOI: 10.11817/j.issn.1672-7347.2016.03.012
Abstract: 目的:观察胸腔镜下T4交感神经干切断术治疗原发性手足多汗症后对足汗的影响。方法:收集2009年6月至2014年5月接受胸腔镜下双侧T4交感神经干切断术治疗原发性手汗症患者28例。在手术前后患者完成有关多汗症的调查问卷并予以评分。术后1个月至半年连续随访足汗症状缓解情况。结果:患者均顺利完成双侧同期胸腔镜下T4交感神经干切断术。术后所有病例手汗症状完全缓解,随访期间未发现手汗症复发。术后1个月足汗症状缓解率28.6%(8/28),但随后足汗的症状再次复发;术后6个月,无患者表现足汗的症状缓解。27例(96.4%)患者对于手术效果非常满意,1例(3.6%)患者认为手术效果比较满意,没有患者不满意或后悔接受手术治疗。结论:胸腔镜下T4交感神经干切断术虽然早期缓解部分原发性手足多汗症患者足汗症状,但效果不能长久维持;不能用该术式来治疗单纯的足汗症
Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.)
Lijun Qiu, Chun Yang, Bo Tian, Jun-Bo Yang, Aizhong Liu
BMC Plant Biology , 2010, DOI: 10.1186/1471-2229-10-278
Abstract: In total, 18,928 publicly available non-redundant castor bean EST sequences, representing approximately 17.03 Mb, were evaluated and 7732 SSR sites in 5,122 ESTs were identified by data mining. Castor bean exhibited considerably high frequency of EST-SSRs. We developed and characterized 118 polymorphic EST-SSR markers from 379 primer pairs flanking repeats by screening 24 castor bean samples collected from different countries. A total of 350 alleles were identified from 118 polymorphic SSR loci, ranging from 2-6 per locus (A) with an average of 2.97. The EST-SSR markers developed displayed moderate gene diversity (He) with an average of 0.41. Genetic relationships among 24 germplasms were investigated using the genotypes of 350 alleles, showing geographic pattern of genotypes across genetic diversity centers of castor bean.Castor bean EST sequences exhibited considerably high frequency of SSR sites, and were rich resources for developing EST-SSR markers. These EST-SSR markers would be particularly useful for both genetic mapping and population structure analysis, facilitating breeding and crop improvement of castor bean.Castor bean (Ricinus communis L., Euphorbiaceae, 2n = 20) is an important non-edible oilseed crop and its seed derivatives are often used in aviation oil, lubricants, nylon, dyes, inks, soaps, adhesive and biodiesel. Among all the vegetable oils, castor bean oil is distinctive due to its high level of ricinoleic acid (over 85%), a fatty acid consisting of 18 carbons, a double bond between C9 and C10, and a hydroxyl group attached to C12. Ricinoleic acid is responsible for castor bean oil interest, with the highest and most stable viscosity index among all the vegetable oils combined with high lubricity, especially under low-temperature conditions. Although it was found that castor bean seeds had been used by people dating from about 4000 BC [1], it is still an unanswered question about the origin of castor bean cultivation. Castor bean's contemporary
Anaerobic benzene biodegradation by a pure bacterial culture of Bacillus cereus under nitrate reducing conditions

Junfeng Dou,Aizhong Ding,Xiang Liu,Yongchao Du,Dong Deng,Jinsheng Wang,

环境科学学报(英文版) , 2010,
Abstract: A pure culture using benzene as sole carbon and energy sources was isolated by screening procedure from gasoline contaminated soil. The analysis of the 16S rDNA gene sequence, morphological and physiological characteristics showed that the isolated strain was a member of genus Bacillus cereus. The biodegradation performance of benzene by B. cereus was evaluated, and the results showed that benzene could be e ciently biodegraded when the initial benzene concentration was below 150 mg/L. The metabolites of anaerobic nitrate-dependent benzene oxidation by strain B. cereus were identified as phenol and benzoate. The results of substrate interaction between binary combinations for benzene, phenol and benzoate showed that the simultaneous presence of benzene stimulated the degradation of benzoate, whereas the addition of benzene inhibited the degradation of phenol. Benzene degradation by B. cereus was enhanced by the addition of phenol and benzoate, the enhanced e ects were more pronounced at higher concentration. To our knowledge, this is the first report that the isolated bacterial culture of B. cereus can e ciently degraded benzene under nitrate reducing conditions.

Li Aizhong,Liu Xuhua,

软件学报 , 1995,
Abstract: In order to describe the discovery process of synthesis and analysis,a composition-decomposition based approach to machine discovery is presented in the paper, which defines the concepts of composition--decomposition operator, composition-decomposition function, function discovery, function maintenance and so on, and this paper also gives a function discovery algorithm and a function maintenance algorithm. As an application of the approach, the process of functional knowledge discovery is studied in terms of functiondiscovery and function maintenance.
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