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Search Results: 1 - 10 of 406618 matches for " Aires M.;Marques "
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Esporos e toxinas de Clostridium botulinum dos tipos C e D em cacimbas no Vale do Araguaia, Goiás
Souza, Aires M.;Marques, Dercino F.;D?bereiner, Jürgen;Dutra, Iveraldo S.;
Pesquisa Veterinária Brasileira , 2006, DOI: 10.1590/S0100-736X2006000300001
Abstract: the occurrence and distribution of clostridium botulinum spores and toxins type c and d in 300 ponds, used by cattle for drinking on 130 farms located in 12 municipalities of the rio araguaia valley, state of goiás, brazil, was evaluated. the presence of spores was determined indirectly by cultivation in culture medium, followed by inoculation and neutralization in mice of samples of the sediment from the bottom of the raining ponds, from superficial soil and from cattle feces collected to its circuit. the toxin presence was evaluated directly by inoculation in mice of the filtered sediment of the ponds, followed by the neutralization in mice with antitoxins c and d. the presence of c. botulinum spores was significantly more frequent (p<0,05) in the cattle feces (31%), when compared with the results of the superficial soil samples (19%) and the sediments (10%). botulinum toxins of type c and d or classified as belonging to the cd compound were detected in 6 samples (2%) of the 300 ponds. of the 130 worked farms, in 122 (93,85%) ponds clostridium botulinum spores or toxins were found in at least one of the researched variables, whilst ponds on only 8 (6,15%) farms did not present any contamination. age and depth of the ponds were associated with the frequency of detection of botulinum spores and toxins. the older and shallower the ponds were, the larger was the frequency of isolation of the spores and toxins. the contamination of the ponds in the araguaia valley with clostridium botulinum spores and toxins type c and d demonstrates the permanent and growing potential risk for the occurrence of botulism in cattle through drinking water.
Esporos e toxinas de Clostridium botulinum dos tipos C e D em cacimbas no Vale do Araguaia, Goiás
Souza Aires M.,Marques Dercino F.,D?bereiner Jürgen,Dutra Iveraldo S.
Pesquisa Veterinária Brasileira , 2006,
Abstract: Foram avaliadas a ocorrência e distribui o de esporos e toxinas de Clostridium botulinum tipos C e D em 300 cacimbas empregadas como bebedouro de bovinos em 130 propriedades rurais localizadas em 12 municípios do Vale do Araguaia, Estado de Goiás. A presen a de esporos foi determinada indiretamente pelo cultivo em meio de cultura, seguido da inocula o e neutraliza o em camundongo das amostras de sedimento do interior das cacimbas, e do solo superficial e fezes de bovinos, coletadas ao seu redor. A presen a de toxina foi avaliada diretamente pela inocula o em camundongo do sedimento filtrado das cacimbas, também seguida da neutraliza o em camundongo com antitoxinas C e D. A presen a de esporos de C. botulinum foi significativamente maior (p<0,05) nas fezes de bovinos (31%), quando comparadas com os resultados das amostras de solo superficial (19%) e dos sedimentos (10%). Foram detectadas toxinas botulínicas dos tipos C, D, ou classificadas como pertencentes ao complexo CD, em seis amostras (2%) das 300 cacimbas. Das 130 propriedades trabalhadas, em 122(93,85%) foram encontrados esporos ou toxinas de Clostridium botulinum em pelo menos uma das variáveis pesquisadas, enquanto somente 8(6,15%) n o apresentaram qualquer contamina o A idade e profundidade das cacimbas estiveram associadas com a freqüência de detec o de esporos e toxinas. Assim, quanto mais velhas e rasas, maior a freqüência do isolamento de esporos e toxinas. A contamina o das cacimbas do Vale do Araguaia goiano com esporos e toxinas do Clostridium botulinum tipos C e D demonstra o risco potencial permanente e crescente para a ocorrência da intoxica o botulínica de origem hídrica nos bovinos.
Plantas úteis para revestimento do solo: II - Gramíneas de porte médio e grande
Alencar, F. M. Aires de;
Bragantia , 1952, DOI: 10.1590/S0006-87051952000400004
Abstract: preliminary results have been obtained on the development of 11 types of grasses with average height varying from 0.70 to 1.15 m and from 11 other types with average height from 1.30 to 5.00 m. from plots with an area of 25 m2, from the collection of useful plants of the soil conservation department of the instituto agron?mico de campinas, one sample of the plants was taken for each plot the sampled area being of 0,50 and 1.00 m2, for the grasses of medium and large size, respectively. the above-ground parts of the plants were cut at the heights of 80, 26, 8, 2 and 0 cm (ground level) respectively and determinations were made of the weight and volume of each portion of the plants under investigation. the results obtained are presented in tables 1 to 3. the underground parts of the grasses were studied in the same areas where the aerial parts were cut. blocks of soil were taken with depths of 2, 8, 26 and 80 cm. the underground portions of the plants in each block were separated by the dry method, using wire screen, their weight and volume being determined (table 1 to 3). the soil particles holding capacity of each species of grass was determined by adding to the weight of the above-ground part at the lowest cut (block aa) the weight of the underground portion found at the first block (block ba, table 2 and 3). it has been observed that among the medium sized grasses the capim chor?o (eragrostis curvula nees var. valida stapf), capim de boi (setaria poiretiana kunth), capim araguai (paspalum fasdculatum willd.) and capim de planta (panicum purpurascens raddi) and among the taller grasses the capim sempre-verde (panicum maximum jacq. var. gongylodes), capim elefante merker, napier and axb (pennisetum purpureum schum.) as well as capim vetiver (vetiveria zizanioides nash), seem to be more promissing for holding the soil particles and more useful for soil conservation purposes.
Plantas úteis para o revestimento do solo pesquisa acêrca das suas caraterísticas de cobertura e travamento
Alencar, F. M. Aires de;
Bragantia , 1949, DOI: 10.1590/S0006-87051949000200004
Abstract: this paper describes the investigations carried out and the methods employed to determine the characteristics of ten plant species for use in various aspects of soil erosion control. seed of the ten plant species to be studied were sowed in beds. after a suitable period of time for growth of the plants, sample areas 0.25 sq. mt. were selected. the number of plants and total volume and weight of the plant growth at specific levels (0-2 cm, 2-8 cm, 8-26 cm, 26-80 cm) both above and below ground were measured. the results obtained have led to the following preliminary conclusions. of the plants studied the species paspalum notalum flügge, composed of two varieties, large leafed batatais and bahia grass, were found to have the largest volume and weight of plant growth in the area 2 to 8 centimeters above and below the soil surface. ro?as grass (paspalum dilatatum poir) proved to be a good ground cover but root penetration and development was relatively poor at all depths. the kikuiu grass (pennisetum clandes-tinum chiov.), tio pedro grass (paspalum convexum h. b.) and gengibre grass (paspalum maritinum trin.) produced a good ground cover but root development in the soil was only fair. the bermuda grass (cynodon dactylon pers.) and portuguêsa grass (panicum repens l.) gave measurements of volume and weight of plant growth at all levels both above and below ground which indicated that these plants would be relatively satisfactory in erosion control work. the data obtained from the study of margaridinha (wedelia paludosa l.) indicated this species is very good both from standpoint of a cover crop and in its capacity to hold the soil in place. the common kudzu (pueraria thumbergiana benth.) gave measurements of top and root development that show that it is a very good species. the author discusses in a general way all of the above mentioned species in relation to specific uses in soil conservation.
Eficácia da associa??o de dexametasona à antibioticoterapia em pacientes pediátricos com meningite bacteriana
Bernardo, Wanderley Marques;Aires, Felipe Toyama;Sá, Fernando Pereira de;
Revista da Associa??o Médica Brasileira , 2012, DOI: 10.1590/S0104-42302012000300011
Abstract: objective: to evaluate the efficacy of the association of corticosteroids and the standard treatment of bacterial meningitis in pediatric patients. methods: a systematic review of the literature was conducted through the medline database. only randomized controlled trials comparing dexamethasone with placebo in the treatment of pediatric patients with bacterial meningitis were included. results: eight articles met the inclusion criteria and were selected for analysis. there were no difference in mortality (p = 0.86), and incidence of neurological (p = 0.41) and auditory (p = 0.48) sequelae between the groups. conclusion: there are no benefits in associating corticosteroids with the standard treatment of bacterial meningitis in pediatric patients.
ANP impairs the dose-dependent stimulatory effect of ANG II or AVP on H+-ATPase subcellular vesicle trafficking  [PDF]
M. Oliveira-Souza, P. Morethson, G. Malnic, M. Mello-Aires
Open Journal of Molecular and Integrative Physiology (OJMIP) , 2013, DOI: 10.4236/ojmip.2013.33015

The effect of angiotensin II (ANG II) or arginine vasopressin (AVP) alone or plus atrial natriuretic peptide (ANP) on H+-ATPase subcellular vesicle trafficking was investigated in MDCK cells following intracellular pH (pHi) acidification by exposure to20 mMNH4Cl for 2 min in a Na+-free solution containing Schering 28080, conditions under which H+-AT-Pase is the only cell mechanism for pHi recovery. Using the acridine orange fluorescent probe (5mM) and confocal microscopy, the vesicle movement was quantified by determining, for each experimental group, the mean slope of the line indicating the changes in apical/basolateral fluorescence density ratio over time during the first 5.30 min of the pHi recovery period. Under the control conditions, the mean slope was 0.079 ± 0.0033 min-1 (14) and it increased significantly with ANG II [10-12 and 10-7 M, respectively to 0.322 ± 0.038 min-1 (13) and 0.578 ± 0.061 min-1 (12)] or AVP [10-12 and 10-6 M, respectively to 0.301 ± 0.018 min-1 (12) and 0.687 ± 0.049 min-1 (11)]. However, in presence of ANP (10-6 M, decreases cytosolic free calcium), dimethyl-BAPTA/AM (5 × 10-5 M, chelates intracellular calcium) or colchicine (10-5 M, 2-h preincubation; inhibits microtubule-dependent vesicular trafficking) alone or plus ANG II or AVP the mean slopes were similar to the control values, indicating that such agents blocked the stimulatory effect of ANG II or AVP on

Aduba??o N-P-K e o desenvolvimento, produtividade e qualidade dos frutos do abacaxi 'gold' (MD-2)
Guar?oni M, André;Ventura, José Aires;
Revista Brasileira de Ciência do Solo , 2011, DOI: 10.1590/S0100-06832011000400031
Abstract: the cultivar gold (md-2) has attracted interest in brazil, with a view to fresh pineapple export. however, little scientific information is available on the nutritional management of this cultivar in the country. this study aimed to determine the effect of n, p and k fertilization on the nutritional status, development, yield and fruit quality of md-2 pineapple plants. five doses of n; p2o5 and k2o, were studied and the leaf 'd' development, the n, p and k levels, and fruit development and quality were evaluated. the yield and fruit weight were highest after the application of 650.6 kg ha-1 n and 735.9 kg ha-1 k2o, corresponding to 12.7 and 14.4 g/ plant, respectively; in this case, flowering should be induced when the 'd' leaf length is > 75.5 cm; the fruit quality was reduced with application of n, but increased with the addition of p and k, up to a maximum of 205.8 kg ha-1 p2o5 and 703.4 kg ha-1 k2o, corresponding to 4.01 and 13.7 g/plant, respectively.
Evaluation of Biological Value and Appraisal of Polyphenols and Glucosinolates from Organic Baby-Leaf Salads as Antioxidants and Antimicrobials against Important Human Pathogenic Bacteria
Alfredo Aires,Esperan?a Marques,Rosa Carvalho,Eduardo A. S. Rosa,Maria J. Saavedra
Molecules , 2013, DOI: 10.3390/molecules18044651
Abstract: The present investigation has been carried out to investigate the biological role of four different types of baby-leaf salads and to study their potential as natural sources of antioxidants and antimicrobials against several isolates from important human pathogenic bacteria. Four single types of salads (green lettuce, red lettuce, rucola and watercress) and two mixtures [(1) red lettuce+green lettuce; (2) green lettuce + red lettuce + watercress + rucola] were assayed. The HPLC analysis revealed interesting levels of polyphenols and glucosinolates. The results showed a significant variation ( p < 0.05) of polyphenols and glucosinolates with plant material. Nine different types of polyphenols grouped in three major classes were found: gallic acid, chlorogenic acid, caffeic acid and dicaffeoyltartaric acid (phenolic acids); quercitin-3- O-rutinoside, quercitin-3- O-rhamnoside, luteolin-7- O-glucoside and isorhamnetin (flavonoids); and cyanidin-3-glucoside (anthocyanins). Only three different glucosinolates were found: glucoraphanin; gluconasturtiin and 4-methoxy-glucobrassicin. A positive correlation was detected between polyphenol contents and antioxidant activity. Red lettuce and mixture 1 were the baby-leaf salads with the highest antioxidant potential. As for the antimicrobial activity, the results showed a selective effect of chemicals against Gram-positive and Gram-negative bacteria and Enterococcus faecalis and Staphylococcus aureus were the bacteria most affected by the phytochemicals. Based on the results achieved baby-leaf salads represent an important source of natural antioxidants and antimicrobial substances.
Control Method of the DFIG Connected to a DC Link through a Diode Bridge  [PDF]
G. D. Marques, M. F. Iacchetti
Energy and Power Engineering (EPE) , 2013, DOI: 10.4236/epe.2013.54B020
Abstract: This paper presents a control method for the Doubly-fed Induction Generator connected to a dc link through a diode bridge on the stator. In this system, the rotor is fed, at the slip frequency, by a PWM electronic converter and the stator is directly connected to the dc link using a simple diode bridge. The cost of power electronics is reduced in this system when compared with the classic DFIG machine because the system uses less one PWM inverter and additionally it uses a diode bridge. The application in mind is for microgrids. Microgrids need several elements that should work together. The usual way to connect these elements is to use power electronic devices in a common dc link. This paper presents a new form for the DFIG for this application and presents a control system for the inner control loop. Simulation and experimental results show that the system can work acceptably using a stator frequency near the rated frequency of the machine.
Botulismo em bovinos de corte e leite alimentados com cama de frango
Dutra, Iveraldo S.;D?bereiner, Jürgen;Souza, Aires M.;
Pesquisa Veterinária Brasileira , 2005, DOI: 10.1590/S0100-736X2005000200009
Abstract: outbreaks of botulism caused by type c and d of the botulinum toxin are frequent in brazil, and are associated with bone chewing and ingestion of contaminated food and water. this paper reports the epidemiological, clinical, pathological and laboratorial aspects of 7 outbreaks of botulism in beef and dairy cattle fed with poultry litter, which occurred in the states of s?o paulo and minas gerais, 1989-2000. five outbreaks occurred in beef cattle herds, raised in confinement or under pasture conditions and supplemented with poultry litter, and 2 outbreaks occurred in dairy farms. from o total of 1,535 cattle supplemented regularily with poultry litter 455 animals (29.64%) died within 2 to 4 weeks. morbidity and mortality varied from 3.47 to 100% in the 7 outbreaks. in one of the farms the lethality was 60.52%, and in others more than 88.43%, reaching 100% in three farms. clinical signs were progressive paralysis, difficulties in moving, decubitus, normal alertness, decreased muscular tonus of tongue and tail, sialorrhoe and dyspnoe. at post-mortem examination of 30 cattle no noteable changes were observed. spores of clostridium botulinum were found in poultry litter samples collected on 7 farms. in liver, ruminal and intestinal fluid samples from 30 necropsied cattle botulinum toxin of type c (5) and d (9) or of the cd complex (1) were found in at least one of the samples collected from 15 animals, which confirms the clincial, pathological and epidemiological diagnosis of botulism.
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