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匹配条件: “ Airborne Microflora” ,找到相关结果约553条。
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Hospital Delivery Room versus Outdoor Birthing Place: Differences in Airborne Microorganisms and Their Impact on the Infant  [PDF]
Tobias C. Olofsson, Alejandra Vàsquez
Open Journal of Medical Microbiology (OJMM) , 2013, DOI: 10.4236/ojmm.2013.31005
Abstract:

The incidence of allergic airway diseases continues to increase in industrial countries while remaining much more stable in developing countries. Allergens inhaled are eventually also swallowed and evidently the gastrointestinal immune system has a role in regulating allergic responses in the pulmonary as well as the GI system. While some studies have pointed out the role of probiotic bacteria as a supplementary protection against the early development of various allergies, little attention has been paid to the composition of the airborne microflora first and continuosly inhaled by newborns and infants. This study compares the composition of two airborne microbial communities, one from hospital delivery rooms and the other from a nature reserve, evidently in use as a birthing place as early as 7500 B.C. around the air from the outdoor birthing place was marked by a far greater variation in microbial composition and a much higher representation of fungi than the air from the hospitals. The dominant bacterial species from the delivery rooms were Staphylococcus areus and Micrococcus luteus, originating from the staff and the hospital environment; the outdoor flora, however, was dominated by Pseudomonas spp. and Bacillus spp. In addition, 56% of all the bacterial isolates from the delivery rooms were most closely related to strains previously associated with clinical infections, whereas only 15% of isolates in the outdoor bacterial sample had such relationships. The role of airborne microorganisms could be important to infants with developing immune systems considering the microbial bias of hospital air presented in this study.

Microbiological Identification of Atmospheric Particles in Hermosillo, Sonora, México  [PDF]
Santos-Romo Alvaro, Sau-Acosta Nancy Janeth, Certucha-Barragán María Teresa, Almendáriz-Tapia Francisco Javier, Monge-Amaya Onofre, Hernández-López Jorge, Zepeda-Ibarra Joel
Journal of Environmental Protection (JEP) , 2014, DOI: 10.4236/jep.2014.55041
Abstract:

The air quality in many cities has been deteriorating and affecting population’s health. Airborne particles can block the defense mechanisms of respiratory system and may lead biological elements such as pollen, bacteria, fungi and viruses into the lungs. This paper presents the study of biologically contaminated air in the city of Hermosillo, analyzing filters containing particulate matter with a diameter equal or less than 10 micrometers (PM10) and Total Suspended Particles (TSP). Three sampling periods were analyzed during 2011. Samples taken from the filters were placed in tubes containing nutritive broth BHI (Brain Heart Infusion) and incubated at 37 °C for 24 hours. The identification of the isolated microorganisms was done by a biochemical test recommended in Bergey’s Manual. Results showed a high bacteria concentration in the presence of microorganisms such as Klebsiella pneumoniae sub pneumoniae, Citrobacter, Enterococcus faecalis, Proteus penneri, Butiauxellaagrestis, Salmonella paratyphi A and Enterobacter sacazakii. When bacterial concentration was analyzed separately, the lowest number of bacteria was observed during February for the south location and in August for the north location. The highest degree of bacterial air pollution was found in the central area of the city.

Relationship of Dietary Soy Protein to Daidzein Metabolism by Cultures of Intestinal Microfloras from Monkeys  [PDF]
Fatemeh Rafii, John B. Sutherland, Brad M. Bridges, Miseon Park, Michael R. Adams
Food and Nutrition Sciences (FNS) , 2012, DOI: 10.4236/fns.2012.32039
Abstract: Soybeans have been shown to contain larger concentrations of isoflavones than other plant foods. The colonic micro-floras of some individuals metabolize isoflavones, including the soy phytoestrogen daidzein, to compounds with altered estrogenic activity that may affect health. Monkeys have been used as models to predict the effect of colonic microorganisms on the metabolism of phytoestrogens. We studied the effect of consumption of a diet rich in soy protein on the metabolism of added daidzein by the intestinal microfloras of monkeys. The metabolism of daidzein by cultures of the colonic microfloras from eight males and eight females of Macaca fascicularis, 6 - 12 years old, consuming diets containing either soy or casein, and two males and three females of Macaca nemestrina, 3 - 5 months old, consuming infant formula, was investigated using high-performance liquid chromatographic analyses. Cultures from ten of the 16 adult monkeys and all five infant monkeys metabolized the added daidzein within 24 h. Daidzein was metabolized within 48 h by cultures from five other monkeys, but it remained even after 72 h in a culture from one female monkey on a casein diet. Equol and dihydrodaidzein were the only metabolites found. Individual variation among monkeys in the efficiency of daidzein metabolism was observed, but there appeared to be no correlation between diet and daidzein metabolism by the intestinal microflora. The intestinal microfloras of most monkeys tested were efficient in the biotransformation of daidzein to equol, regardless of the animals’ consumption of soy protein. Differences in the metabolism of isoflavones by the colonic microfloras of humans and experimental animals should be considered when extrapolating results from animals to humans.
Dynamism of Antimicrobial Activity of Actinomycetes—A Case Study from Undisturbed Microbial Niche  [PDF]
Jayshree D. Patel, Mahesh Parmar, Purshottam Patel, Pravin Rohit, Ramesh Taviyad, Parveen Ansari, Bipasha Bhattacharya, Dhaval Vyas, Vipin Kumar, Nirmal S. Sahay, Pawan K. Singh
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.46039
Abstract:

Escalation of drug resistant microbes (bacteria) had forced researchers to search new and improved therapeutic compounds from different possible sources, including metabolites secreted by the actinomycetes. The aim of this study was to evaluate the pattern of antimicrobial actinomycetes from physiologically distinct soil of different geographical locations. Forty five soil samples were collected from 5 districts of Gujarat including two sanctuaries as source of survey for bioactive actinomycetes. Crowded plate technique was used for isolation and Agar cylinder method was employed for the antimicrobial screening. A total of 171 actinomycetes were isolated and screened against eighteen pathogens responsible for causing diseases in plants and humans. Results indicate that 79% of the isolates were active against at least one of the eighteen tested pathogens. Some of the actinomycetes strain had shown strong antibacterial and antifungal activity which may be a good source of obtaining novel antimicrobials.

Microbiota indígena do meato acústico externo de gatos hígidos
Amaral, Ricardo Coutinho do;Iba?ez, José Fernando;Mamizuka, Elsa Masae;Gambale, Walderez;Paula, Claudete Rodrigues de;Larsson, Carlos Eduardo;
Ciência Rural , 1998, DOI: 10.1590/S0103-84781998000300015
Abstract: the study aimed to find out the frequency of microrganisms in the external ear canal in healthy cats. fifty adult male (26) and female (24) short hair cats were utilized for the purpose, and they were divided according to living enviroment (group l - indoor and group 2 - outdoor). all animals were submitted to sedation prior to physical and otoscopic examinations in order to confirm to be healthy. samples were obtained from ear canal by use of buck curetes and sterile cotton sticks for microbiological investigation. in cats of group 1, otodects cynotis were observed in 4%. in 72% of the animals yeasts and molds were found (cladosporium sp 66.6%, malassezia sp 40%, penicillium sp 33%, aspergillus sp 33.3%, rhodotorula sp 20%, mycelia sp 13.3%, and 6.6% for alternaria sp, aureobasidium sp, ryzopus sp and trichosporon sp), finally, m 64% of the samples, was detected staphylococcus spp (81.2%), pseudomonas sp (12.5%), klebsiella sp (12.5%), and 6,2% for acinetobacter sp. bacilos difteroides, enterobacter sp, lactobacillus spp. in group 2, o. cynotis was isolated in 36% of cats, and in 96% of the animals wasfound malassezia sp (54.1%), aspergillus sp (33.3%), penicillium (33.3%), microsporum sp (29.1%), cladosporium sp (16.6%), trichoderma sp (12.5%) alternaria sp (8.3%), phoma sp (8.3%), epicoccum sp (4.1%), neurospora sp (4.1%), mycelia sp (4.3%), rhodotorula sp (4.1%). in 20 out of 25 (80%), at least one bacterial microrganism was isolated (staphylococcus sp 75%, klebsiela sp 20.8%, bacilos difteroides 12.5%, pseudomonas sp 8.3%, and 4.1% for acynetobacter sp, enterobacter and escherichia sp).
Papel de la flora intestinal en la salud y en la enfermedad
Guarner,F.;
Nutrición Hospitalaria , 2007,
Abstract: the terms intestinal "microflora" or "microbiota" refer to the microbial ecosystem colonizing the gastrointestinal tract. recently developed molecular biology instruments suggest that a substantial part of bacterial communities within the human gut still have to be described. the relevance and impact of resident bacteria on the host physiology and pathology are, however, well documented. the main functions of intestinal microflora include (1) metabolic activities translating into energy and nutrients uptake, and (2) host protection against invasion by foreign microorganisms. intestinal bacteria play an essential role in the development and homeostasis of the immune system. lymphoid follicles within the intestinal mucosa are the main areas for immune system induction and regulation. on the other hand, there is evidence implicating intestinal microbiota in certain pathological processes including multi-organ failure, colon cancer, and inflammatory bowel disease.
Microorganismos presentes en el reverso de las u?as de trabajadores de la salud, Hospital Max Peralta, Cartago, Costa Rica
Hernández-Chavarría,Francisco; Alvarado,Karina; Madrigal,Warren;
Revista Costarricense de Ciencias Médicas , 2003,
Abstract: hand cleansing remain the most effective and least expensive measure to prevent transmission of nosocomial infections; however, it is a practical that sometimes is neglected and permits the establishment of bacteria in fingernails, that could acts as a reservoir for those agents. for that reason, the aim of this study was the evaluation of the microflora of subungual region in health care workers from costa rican hospital. culture obtained from 46 health workers from the material took under their subungual region; which was inoculated on blood agar. from 48% of these persons were isolated at least one microorganism. the most frequent agent cultured was staphylococcus (12% s. aureus 24%, s. epidermidis and 22% s. warneri), that correspond to the 75% of the isolations; also, 3 strains of candida (c. tropicalis, c. guillermondi and c. parapsilopsis) were cultivated. these data reaffirm the importance of conscientious hand cleansing to preventn osocomial infections.
Estudo da microflora do megaes?fago chagásico
Crema, Eduardo;Madureira, Andréa Berdu;Lima, Vanessa Guimar?es de Freitas;Castro, Ana Márcia Werneck de;Silva, Alex Augusto;Junqueira, Iracema Saldanha;
Revista da Sociedade Brasileira de Medicina Tropical , 2002, DOI: 10.1590/S0037-86822002000100008
Abstract: chagasic patients with megaesophagus were submitted to an endoscopy of the upper digestive tract and the samples were collected with special instruments under sterilized conditions. one of the four samples collected was from the stase liquid and the other three samples were collected from fragments of the esophageal mucosa at one, three and five centimeters from the esophageal-stomach transition (z line). the samples were analized by the microbiology and pathologic labs for the identification of microorganisms. after that, the results were correlated with the degree of mega esophagus according to ferreira-santos. we observed that the incidence of pathogenic microorganism is very high in megaesophagus, with no relation with the degree of dilatation making the surgery for the treatment of this afection potencially contaminated. there was no significant difference concerning the positivity of the culture relating to the degree of esophagus dilatation.
Effect of a single-use toothbrush on plaque microflora
Pai Vidya
Indian Journal of Dental Research , 2009,
Abstract: Aim: To study and compare the microbial flora of dental plaque after the use of a self-contaminated toothbrush and that of a single-use toothbrush. Materials and Methods: The study group included 40 young volunteers from Yenepoya Dental College, who were free from any systemic or oral disease. In these subjects, plaque samples were collected after 1 month use of a self-contaminated toothbrush. Each subject was given a set of 30 new toothbrushes and a toothpaste tube and instructed to use one toothbrush everyday and discard it after use. The plaque samples were collected on a weekly interval and cultured on Mitis Salivarius agar. The colonies were identified and speciated and their count was recorded. Results: Streptococcus mitis, S. mutans, S. sanguis, S. milleri and Candida were recovered from the samples. A highly significant decrease in their numbers was found after the use of a single-use toothbrush (P value 0.001). Conclusions : As a contaminated toothbrush can reintroduce microorganisms into the oral cavity, it may be a sound practice to change the toothbrush as frequently as possible.
A simple and rapid method for extracting bacterial DNA from intestinal microflora for ERIC-PCR detection
Jin-Long Yang, Ming-Shu Wang, An-Chun Cheng, Kang-Cheng Pan, Chuan-Feng Li, Shu-Xuan Deng
World Journal of Gastroenterology , 2008,
Abstract: AIM: To develop a simple and convenient method for extracting genomic DNA from intestinal microflora for enterobacterial repetitive intergenic consensus (ERIC)-PCR detection.METHODS: Five methods of extracting bacterial DNA, including Tris-EDTA buffer, chelex-100, ultrapure water, 2% sodium dodecyl sulfate and 10% Triton-100 with and without sonication, were compared with the commercial fecal DNA extraction kit method, which is considered as the gold standard for DNA extraction. The comparison was based on the yield and purity of DNA and the indexes of the structure and property of micro-organisms that were reflected by ERIC-PCR.RESULTS: The yield and purity of DNA obtained by the chelex method was similar to that obtained with the fecal DNA kit. The ERIC-PCR results obtained for the DNA extracted by the chelex method and those obtained for DNA extracted with the fecal DNA kit were basically the same.CONCLUSION: The chelex method is recommended for ERIC-PCR experiments in view of its simplicity and cost-effectiveness; and it is suitable for extracting total DNA from intestinal micro-organisms, particularly for handling a large number of samples.
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