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Search Results: 1 - 10 of 120152 matches for " Aijian Wang "
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Nonlinear Optical Properties of Novel Polypyrrole Derivatives Bearing Different Aromatic Segments  [PDF]
Wei Zhao, Yun Wang, Aijian Wang
Materials Sciences and Applications (MSA) , 2017, DOI: 10.4236/msa.2017.811056
Abstract: Polypyrrole (PPy) and its derivatives containing different were synthesized by the interfacial polymerization reaction using ammonium per sulfate as oxidant, and were characterized by using Fourier transform infrared, Ultraviolet/visible absorption, and Raman spectroscopic techniques. The third-order nonlinear optical properties of all samples were measured by using nanosecond Z-scan measurements at 532 nm. At the identical mass concentration of 0.15 mg·mL-1, all PPy derivatives exhibited an obvious reverse saturable absorption performance, while the saturable absorption response was observed for PPy. In addition, significant differences in their nonlinear optical performances were observed, highlighting the influence on optical nonlinearity of the aromatic segments of conjugated polymers.
Synthesis and Crystal Structure of the Hydrogen Bromide Salt of 1,4,7,10-Tetrakis(2-((4-methoxy)phenoxy) ethyl)-1,4,7,10-tetraazacyclododecane  [PDF]
Wei Zhao, Yun Wang, Aijian Wang
Journal of Materials Science and Chemical Engineering (MSCE) , 2017, DOI: 10.4236/msce.2017.510001
Abstract: A novel molecule tetra-N-alkylation of cyclen (1,4,7,10-tetraazacyclododecane), 1,4,7,10-tetrakis(2-((4-methoxy)phenoxy)ethyl)-1,4,7,10-tetraazacyclododecane 1, was synthesized and structurally characterized by the single-crystal X-ray diffraction. The crystals were obtained from ethanol by slow evapora-tion at room temperature and the four hydroquinone groups of the benzene ring formed a π-electron-rich cavity by C-H···Br stacking interaction. The crystal belongs to the orthorhombic system, space group Pbcn with a = 17.3174(15), b = 12.9891(11), c = 19.3379(17) ?, α= β = γ = 90°, V = 4349.8(7) ?3, Z = 4, Dc = 1.304 g/cm3, C44H61 BrN4O8, Mr = 853.88, F(000) = 1808, μ = 1.001 mm?1, CuKa radiation (λ = 0.71073), R = 0.0434 and wR = 0.1091 for 5200 observed reflections with I > 2σ(I).
A Polypyrrole Hybrid Material Self-Assembled with Porphyrin: Facial Synthesis and Enhanced Optical Limiting Properties  [PDF]
Yun Wang, Aijian Wang, Peiyou Yang, Wenxiu Hu, Xingnan Guo, Jing Zhang, Cheng Li, Chi Zhang
Journal of Materials Science and Chemical Engineering (MSCE) , 2017, DOI: 10.4236/msce.2017.510003
Abstract: Polypyrrole/porphyrin nanohybrid (PPy/Tpp(OH)4 nanohybrid) have been synthesized through a self-assembled approach, and the assynthesized PPy/Tpp(OH)4 nanohybrid are characterized by Fourier-transform infrared, X-ray photoelectron spectroscopy, Raman spectroscopy, thermogravimetric analysis, Ultraviolet-visible absorption, scanning electron microscopy, and steady state fluorescence spectroscopic techniques. Formation of the PPy/Tpp(OH)4 nanohybrid dramatically improved the solubility and processability of the PPy-based nanomaterial. The nonlinear optical (NLO) properties of PPy/Tpp(OH)4 nanohybrid were measured by Z-scan at 532 nm with nanosecond laser pulse, the results indicating that PPy/Tpp(OH)4 nanohybrid exhibits a enhanced NLO property in comparison with the benchmark PPy and Tpp(OH)4 due to a combination of mechanisms.
Electrosynthesis of poly(o-phenylenediamine) in ionic liquid and its properties
YanFang Du,Huan Wang,AiJian Zhang,JiaXing Lu
Chinese Science Bulletin , 2007, DOI: 10.1007/s11434-007-0331-9
Abstract: Ionic liquid 1-ethyl-3-methylimidazolium bromide ([EMIM]Br) and N-methylimidazolium tetrafluoroborate ([HMIM]BF4) have been used as solvent and electrolyte for the electropolymerization of o-phenylenediamine at glassy carbon electrode by cyclic voltammetry. It was found that monomer was more easily oxidized in ionic liquid than acidic aqueous solution, and oxidative potential was reduced by 0.725 and 0.455 V, respectively. Electrode modified with poly(o-phenylenediamine) (PPD) has obvious electrochemical activity in acid solution (pH 1–4). The apparent diffusion coefficient (D app) of PPD film is measured by chronocoulometry and chronoamperometry. The polymer structure is measured by UV and IR. IR spectrometry reveals that poly(o-phenylenediamine) has phenazine ring structure. The heat stability of polymer is observed from thermogravimetry.
Molecular epidemiology of chicken anemia virus in commercial farms in China
Yassir M Eltahir, Kun Qian, Wenjie Jin, Pingping Wang, Aijian Qin
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-145
Abstract: Of 460 spleen samples tested by PCR, 47 (10.22%) were found to be positive for CAV. A total of 25 CAV, approximately full genomes, from different commercial farms were characterized. Phylogenetic analysis of the Chinese CAV sequences together with strains from different countries resulted in four distinct groups (A-D) with significant high bootstrap values. The Chinese viral sequences were located as four different clusters within groups A and D. All the Chinese CAV genomes characterized in this study had glutamine (Q) at amino acid position 394, which indicated that all are highly pathogenic. Mutations associated with attenuation and weaker reactivity with monoclonal antibody 2A9 were absent in the Chinese sequences.We revealed that CAV prevalence was lower than that reported previously in commercial farms in China. We also showed four distinct sequence groups (A-D), and genetic variability in local CAV sequences that could be divided into four groups based on phylogenetic analysis.Chicken anemia virus (CAV), a member of the family Circoviridae, is a non-enveloped, icosahedral virus with a negative-sense, single-stranded circular DNA genome [1]. The CAV genome consists of 2.3 kb, with three partially overlapping open reading frames (ORFs) for VP1, the major viral structural protein (51.6 kDa); VP2, a scaffolding protein (24 kDa); and VP3, a non-structural protein named apoptin (13.6 kDa) for its ability to induce apoptosis; VP1 and VP2 are the main targets of neutralizing antibodies[2]. The VP1 gene has the highest variability of the three overlapping ORFs, according to sequences that have been submitted to GenBank [3].To date, all viruses seem to belong to the same worldwide serotypes. However, because there are currently only a few full genome sequences available for CAV strains from the USA, Asia, Australia and Europe, the emergence of new serotypes cannot be excluded, which would have important consequences for vaccine efficacy and serodiagnosis [4].In China, C
Proteomics analysis of feather pulp from chickens infected with very virulent strain of Marek's disease virus

Xinhong Chen,Zhanjun Lu,Kun Qian,Wenjie Jin,You Wang,Aijian Qin,

微生物学报 , 2011,
Abstract: 摘要:【目的】羽毛是细胞游离马立克氏病病毒(Marek's disease virus,MDV) 释放的部位,为了解感染MDV后鸡羽中宿主基因表达的变化及对病毒感染的应答,进行了MDV 感染鸡的羽髓蛋白质组学分析。【方法】1日龄无特定病原体( specific pathogen free,SPF) 鸡人工感染MDV 超强毒RB1B 株(1000PFU),感染后21d 采集鸡羽毛,提取羽髓蛋白,以17cm,pH5 - 8 的IPG 胶条进行二维电泳,以未感染病毒的SPF 鸡羽髓蛋白为对照,使用PDQuest 软件对二维电泳图谱进行差异蛋白分析,并选取部分差异斑点进行质谱鉴定。【结果】PDQuest 软件分析发现攻毒组和对照组表达差异大于两倍的蛋白点有41 个,其中攻毒组表达上调的蛋白点25 个,下调的蛋白点7 个,新出现的蛋白点有9 个。质谱分析共成功鉴定了21 个斑点,对应于20 个蛋白。如载脂蛋白AI(apolipoprotein AI)、14-3-3 sigma(两个斑点均为该蛋白)、癌蛋白18(stathmin) 等。【结论】功能预测表明这些蛋白涉及到宿主的抗病毒应答、物质代谢、细胞骨架成分、细胞增殖相关等方面。

ZHANG Guirong,Zhang Aijian,Wang Huan,Liu Xiuli,LU Jiaxing,

高分子学报 , 2008,
Abstract: The mechanism of the electro-polymerization of aniline in 0.5 mol/L sulfuric acid was studied in detail by in situ UV-Vis spectrometry.Spectroelectrochemical experiments were done in a three electrode cell built with a quartz cuvette of 1cm path length.The work electrode was an ITO (indium-tin oxide) conductive glass perpendicular to light path,and the counter electrode was a platinum wire.An Ag/AgCl electrode with saturated KCl served as reference electrode.All potential values in the article were against to the reference.The acquired in situ UV-Vis spectra showed that polyanilines could be electropolymerized at aniline concentrations 0.01,0.05 and 0.2 mol/L in sulfuric acid by cyclic voltammetry.Based on in situ UV-Vis spectra corresponding to cylic potential sweep at three different concentration of aniline above,the plots of the relationship between absorbance at 350 nm and 750 nm and elctrolysis time was made,which showed that the obvious inductive period in electropolymerization of aniline by cyclic voltammetry was seen for 0.01 mol/L aniline in 0.5 mol/L sulfuric acid water solution.In order to study how the concerntration of aniline in 0.05 mol/L sulfuric acid and the potential applied on ITO conductive glass electrodes effected the polymerization of aniline,in situ UV-Vis spectra was recorded during electrolysis of solution by potentiostatic method at different concentrations of aniline in 0.5 mol/L sulfuric acid.These in situ UV-Vis spectra indicated that the polymerization of aniline occurred by electrolysis of aniline at potentials 0.8,1.0 and 1.3 V when the concentration of aniline was greater than 0.05 mol/L,however at potential 0.8 V,the polymerization of aniline did not happen in 0.01 mol/L solution of aniline.in situ UV-Vis spectra at the earlier phase of aniline electropolymerization by potentiostatic way showed that the 350 nm absorbance band appeared before the 750 nm absorbance band.This fact proposed that lower molecular weight oligomer intermediates during electrochemical polymerization of aniline might exist and that the intermediate was brought out prior to the production of polyaniline on ITO electrode.When the ITO conductive glass electrode was placed parallel to the light path,no absorbance bands were observed during electrolyzing 0.05 mol/L of aniline at potential 0.8 V,which showed that the intermediate and polymer mainly were produced on the ITO conductive glass electrode.These phenomena was not reported up to now.When the aniline concentration was 0.01 mol/L,and the potential applied on the ITO glass conductive electrode was 0.8 V,there were only an absorbance band at 350 nm in corresponding in situ UV-Vis spectra,which showed that the intermediates such as low molecular oligomers could be produced and exist on the ITO electrode,but did not lead to the formation of polyaniliane because of the low concentration of intermediates.After aniline of 0.05 mol/L in 0.5 mol/L sulfuric acid was electropolymerized by potentio
Kinase domain analysis of MDV-1 CVI988/Rispens UL13 and preferred codon fragments expression in Escherichia coli

Chenfei Zhang,Aijian Qin,Xufang Deng,Yuwen Su,Min Xue,Tianyan Yin,Pingping Wang,

微生物学报 , 2009,
Abstract: 摘要:目的 寻找MDV-1UL13激酶催化中心,并体外表达UL13,以便研究UL13蛋白激酶的功能。方法 用PCR方法从CVI988疫苗株基因组中扩增UL13基因,利用GENEART(www.gcua.de)分析UL13在大肠杆菌中表达时密码子的偏嗜性;通过DNAstar抗原性分析确定UL13的高抗原性片段,进行原核表达,并以切胶免疫方法免疫小鼠制备多抗血清;通过NCBI的protein blast功能及Cn3D 4.1在线软件分析UL13保守结构域。结果 PCR扩增出UL13基因,序列分析结果表明, UL13的259-400aa、431-513aa两个片段抗原性强,且稀有密码子较少;保守结构域分析发现UL13催化中心主要位于152-297氨基酸残基间,且在激酶Subdomain Ⅶ的保守甘氨酸残基被丝氨酸替代,SubdomainⅧ的保守非极性脯氨酸残基被极性半胱氨酸残基替换。利用大肠杆菌表达的UL13第259~400 aa片段免疫小鼠制备出多抗血清能与真核表达产物反应。结论 MDV-1 UL13催化中心主要位于152-297氨基酸残基间,体外表达的基因产物诱导机体产生了抗UL13激酶的特异性抗体。
Analysis of chicken anemia virus genome: evidence of intersubtype recombination
Yassir M Eltahir, Kun Qian, Wenjie Jin, Aijian Qin
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-512
Abstract: Phylogenetic analysis, together with a variety of computational recombination detection algorithms, was used to investigate CAV approximately full genomes. Statistically significant evidence of intersubtype recombination was detected in the parent-like and two putative CAV recombinant sequences. This event was shown to occur between CAV subgroup A1 and A2 sequences in the phylogenetic trees.We revealed that intersubtype recombination in CAV genome sequences played a role in generating genetic diversity within the natural population of CAV.Chicken anemia virus (CAV) was first reported in 1979 in specific-pathogen-free (SPF) chickens [1]. CAV belongs to the Circoviridae and is a non-enveloped, icosahedral virus with a negative-sense, single-stranded circular DNA. The viral genome consists of 2.3 kb, with three partially overlapping open reading frames. CAV infection is an economically important clinical and subclinical disease in broiler chickens, with a worldwide distribution [2].CAV isolates show extremely limited genetic variability worldwide [3]. All isolates of CAV are suspected to belong to a single serotype [4]. Little is known about CAV genome recombination analysis. CAV putative intergenotype recombinants have been reported to occurs in the virus gene VP1 and results in a new virus genotype [5].Recently, after adding more CAV approximately full genome sequences to GenBank, CAV sequences arising from different parts of world have been categorized into four genotype groups (A-D) and five subtypes (A1, A2, A3, D1 and D2) [6]. Therefore, the necessity of exploring these CAV genotypes for evidence of recombination as an important tool for genetic variability has been raised, to establish if any would help in understanding the evolutionary process in the CAV genome.Here, we report evidence of intersubtype recombination based on sequence analysis of the entire coding regions (VP1, VP2 and VP3) of CAV genomes.DNA extraction, PCR screening, amplification of CAV genome
Expression and intercellular trafficking of the VP22 protein of CVI988/Rispens vaccine strain of Marek’s disease virus
HongJun Chen,CuiPing Song,AiJian Qin,ChenFei Zhang
Science China Life Sciences , 2007, DOI: 10.1007/s11427-007-2038-1
Abstract: The viral protein 22 (VP22) in the tegument of Marek’s disease virus serotype 1 (MDV-1) plays an important role in cell-to-cell spread and viral propagation. Antiserum against the carboxyl terminus of VP22 was prepared by immunizing mice with recombinant VP22 expressed in E. coli, and used to investigate its expression in chicken embryo fibroblast (CEF) cells infected with different MDV-1 strains. At an infection dose of PFU=50, intercellular trafficking of the VP22 into the nuclei of the surrounding receipt cells was detected as early as 3 hours post infection. By 6 hours after infection (before viral plague formation), the protein was detected in the whole nuclei of the recipient cells with no difference among MDV-1 strains CVI988/Rispens, GA and RB1B. Intra-nuclear accumulation of the VP22 protein was further increased when the viral plagues started to form. These results indicate that, albeit the existence of the 201TKSERT206 deletion, the VP22 of the CVI988/Rispens vaccine strain has also intercelular-trafficking function, which might serve as a potential alternative delivering protein instead of virulent strains VP22.
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