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Search Results: 1 - 10 of 37860 matches for " Aihua Lin "
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Analysis on the Growth Rhythm and Cold Tolerance of Five-Year Old Eucalyptus benthamii Plantation for Bioenergy  [PDF]
Aihua Yu, Tom Gallagher
Open Journal of Forestry (OJF) , 2015, DOI: 10.4236/ojf.2015.56052
Abstract: A research plot of Eucalyptus benthamii was planted to evaluate this species’ ability to supply the emerging bioenergy markets that are developing in the southern U.S. The plot was planted in two different densities to investigate the growth parameters and the cold tolerance. The stand was measured annually through five growing seasons. The results indicated that the growth difference among the young E. benthamii was noticeable. For example, the maximum and minimum value of five-year old trees at diameter breast height (DBH) was 27.9 centimeters and 1.27 centimeters; and the maximum and minimum value of tree height was 22.86 meters and 2.44 meters, respectively. The yearly change in DBH and height of E. benthamii had significant differences. The average annual survival rates of E. benthamii had differences under the two planting densities (1650 trees ha-1 and 1237 trees ha-1). The densities also had effects on the height and DBH growth of E. benthamii. The average DBH and height of 1650 trees ha-1 plantation were 11.18 centimeters and 15.03 meters, and the average DBH and height of 1237 trees ha-1 plantation were 13.46 centimeters and 16.28 meters. The volume per hectare of 1650 trees ha-1 and 1237 trees ha-1 plantation were 111.45 cubic meters and 101.15 cubic meters, respectively. Average diameter growth was almost 2.54 centimeters per year and average height growth was over 3 meters. E. benthamii plantations were considered tolerant to -7.4 degrees Celsius and a cold spell during early 2014 (-11.3 degrees Celsius for two consecutive nights) killed the plantation. The growth of E. benthamii also
The Association between ATM IVS 22-77 T>C and Cancer Risk: A Meta-Analysis
Lin Zhao,Aihua Gu,Guixiang Ji,Peng Zou,Peng Zhao,Ailin Lu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0029479
Abstract: It has become increasingly clear that ATM (ataxia-telangiectasia-mutated) safeguards genome stability, which is a cornerstone of cellular homeostasis, and ATM IVS 22-77 T>C affects the normal activity of ATM proteins. However, the association between the ATM IVS 22-77 T>C genetic variant and cancer risk is controversial. Therefore, we conducted a systematic meta-analysis to estimate the overall cancer risk associated with the polymorphism and to quantify any potential between-study heterogeneity.
Association of the Maternal MTHFR C677T Polymorphism with Susceptibility to Neural Tube Defects in Offsprings: Evidence from 25 Case-Control Studies
Lifeng Yan, Lin Zhao, Yan Long, Peng Zou, Guixiang Ji, Aihua Gu, Peng Zhao
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041689
Abstract: Background Methylenetetrahydrofolate reductase (MTHFR) is a critical enzyme in folate metabolism and is involved in DNA methylation, DNA synthesis, and DNA repair. In addition, it is a possible risk factor in neural tube defects (NTDs). The association of the C677T polymorphism in the MTHFR gene and NTD susceptibility has been widely demonstrated, but the results remain inconclusive. In this study, we performed a meta-analysis with 2429 cases and 3570 controls to investigate the effect of the MTHFR C677T polymorphism on NTDs. Methods An electronic search of PubMed and Embase database for papers on the MTHFR C677T polymorphism and NTD risk was performed. All data were analysed with STATA (version 11). Odds ratios (ORs) with 95% confidence intervals (CIs) were estimated to assess the association. Sensitivity analysis, test of heterogeneity, cumulative meta-analysis, and assessment of bias were performed in our meta-analysis. Results A significant association between the MTHFR C677T polymorphism and NTD susceptibility was revealed in our meta-analysis ( TT versus CC: OR = 2.022, 95% CI: 1.508, 2.712; CT+TT versus CC: OR = 1.303, 95% CI: 1.089, 1.558; TT versus CC+CT: OR = 1.716, 95% CI: 1.448, 2.033; 2TT+CT versus 2CC+CT: OR = 1.330, 95% CI: 1.160, 1.525). Moreover, an increased NTD risk was found after stratification of the MTHFR C677T variant data by ethnicity and source of controls. Conclusion The results suggested the maternal MTHFR C677T polymorphism is a genetic risk factor for NTDs. Further functional studies to investigate folate-related gene polymorphisms, periconceptional multivitamin supplements, complex interactions, and the development of NTDs are warranted.
Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome
Aihua Wang,Yi Ding,Zhenhua Hu,Chufa Lin,Shuzhen Wang,Bingcai Wang,Hongyuan Zhang,Guolin Zhou
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms130911188
Abstract: In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity ( H E) and observed heterozygosity ( H O) levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species.
The TERT rs2736100 Polymorphism and Cancer Risk: A Meta-analysis Based on 25 Case-Control Studies
Peng Zou, Aihua Gu, Guixiang Ji, Lin Zhao, Peng Zhao, Ailin Lu
BMC Cancer , 2012, DOI: 10.1186/1471-2407-12-7
Abstract: A computerized search of PubMed and Embase database for publications on the TERT rs2736100 polymorphism and cancer risk was performed and the genotype data were analyzed in a meta-analysis. Odds ratios (ORs) with 95% confidence intervals (CIs) were estimated to assess the association. Sensitivity analysis, test of heterogeneity, cumulative meta-analysis and assessment of bias were performed in our meta-analysis.A significant association between the TERT rs2736100 polymorphism and cancer susceptibility was revealed by the results of the meta-analysis of the 25 case-control studies (GG versus TT: OR = 1.72, 95% CI: 1.58, 1.88; GT versus TT: OR = 1.38, 95% CI: 1.29, 1.47; dominant model-TG + GG versus TT: OR = 1.47, 95% CI: 1.37, 1.58; recessive model-GG versus TT + TG: OR = 1.37, 95% CI 1.31, 1.43; additive model-2GG + TG versus 2TT + TG: OR = 1.30, 95% CI: 1.25, 1.36). Moreover, increased cancer risk in all genetic models was found after stratification of the SNP data by cancer type, ethnicity and source of controls.In all genetic models, the association between the TERT rs2736100 polymorphism and cancer risk was significant. This meta-analysis suggests that the TERT rs2736100 polymorphism may be a risk factor for cancer. Further functional studies between this polymorphism and cancer risk are warranted.Cancer is a multifactorial disease, which is the result of complex interactions between inherited and environmental factors. Lung cancer is the most common malignancy and the leading cause of cancer deaths for women and men worldwide [1-3]. There are two main histologic subgroups of lung cancer: small-cell lung carcinoma (SCLC) and non-small-cell lung carcinoma (NSCLC); the latter includes the common types, which are squamous cell carcinoma (SCC) and adenocarcinoma (ADC). Gliomas of astrocytic, oligodendroglial, and ependymal origin are derived from glial cells and account for Fax~80% of malignant primary brain tumors (PBTs), which are the most common histologic type
Predominant porB1A and porB1B genotypes and correlation of gene mutations with drug resistance in Neisseria gonorrhoeae isolates in Eastern China
Aihua Sun, Xingli Fan, Ye Gu, Peng Du, Renxian Tang, Yafei Mao, Xuai Lin, Jie Yan
BMC Infectious Diseases , 2010, DOI: 10.1186/1471-2334-10-323
Abstract: The β-lactamase production of N. gonorrhoeae isolates was determined by paper acidometric test and nitrocefin discs. The porB1A and porB1B genes of 315 non-penicillinase-producting N. gonorrhoeae (non-PPNG) strains were amplified by PCR for sequencing to determine serotypes and site mutations. A duplex PCR was designed to simultaneously detect both porB1A and porB1B genes. Penicillin and tetracycline resistance was assessed by an in vitro drug sensitivity test.Of the N. gonorrhoeae isolates, 31.1% tested positive for porB1A and 68.9% for porB1B genes. All the 98 porB1A+ isolates belonging to IA6 serotype with either no mutation at the 120 and 121 sites (88.8%) or a D120G (11.2%) mutation and were no resistance to both penicillin and tetracycline. Among the 217 porB1B+ isolates, 26.7%, 22.6% and 11.5% belonged to IB3, IB3/6 and IB4 serotypes, respectively. Particularly, two novel chimeric serotypes, IB3/6-IB2 and IB2-IB4-IB2, were found in 77 and 8 porB1B+ isolates. Two hundred and twelve (97.7%) of the porB1B+ isolates were presented G120 and/or A121 mutations with 163 (76.9%) at both sites. Interestingly, within the 77 porB1B+ isolates belonging to IB3/6-IB2 serotype, 15 were discovered to possess novel deletions at both A121 and N122 sites. All the replacement mutations at these sites in PorB1B were correlated with resistance and the deletion mutation showed the highest resistance.N. gonorrhoeae isolates circulating in Eastern China include a sole PorB1A serotype (IA6) and five PorB1B serotypes. Multiple mutations in porB1B genes, including novel A121 and N122 deletions, are correlated with high levels of penicillin and tetracycline resistance.Gonorrhoea caused by infection with Neisseria gonorrhoeae is a global sexually transmitted disease. In Chinese populations, gonorrhoea is the most common sexually transmitted disease which causes a serious public health problem [1-5].The outer membrane of N. gonorrhoeae bears many proteins such as porins that have been studi
Characterization of Conserved Combined T and B Cell Epitopes in Leptospira interrogans Major Outer Membrane Proteins OmpL1 and LipL41
Xu'ai Lin, Aihua Sun, Ping Ruan, Zhe Zhang, Jie Yan
BMC Microbiology , 2011, DOI: 10.1186/1471-2180-11-21
Abstract: In this study, four epitopes from OmpL1 and four from LipL41 conserved regions were evaluated for their potential utilization in leptospire vaccines. Firstly, combined B and T cell epitopes were predicted by softwares and expressed using a phage display system. OmpL1 residues 87-98 and 173-191 (OmpL187-98 and OmpL1173-191) and LipL4130-48, LipL41233-256 of LipL41 were identified as immunodominant B cell epitopes by Western blot. Epitopes OmpL1173-191, OmpL1297-320 of OmpL1 and LipL41233-256, LipL41263-282 of LipL41 were identified as immunodominant CD4+ T cell epitopes through proliferation analysis of splenocytes from recombinant OmpL1 (rOmpL1) or recombinant LipL41 (rLipL41)-immunized BALB/c (H-2d) mice. These epitopes induced responses of CD4+ T cells and Th1 (T helper cells) type cytokine responses during the infection.This work identified combined T and B cell immunodominant epitopes in outer membrane proteins OmpL1 and LipL41 of Leptospira interrogans. OmpL1173-191 of OmpL1 and LipL41233-256 of LipL41 could be useful in a vaccine against Leptospira. The findings could also contribute to the development of effective cross-protective vaccine strategies for leptospirosis.One of the emerging health problems in poor urban slum communities in developing countries is leptospirosis caused by pathogenic Leptospira, which is the most widespread zoonotic disease[1].The immune responses to leptospires appear complex. Both animal model and human clinical studies have indicated that during the infection, leptospires can still persistently present despite robust immune responses suggesting that leptospires are capable of evading both innate and adaptive immunity and the immune responses triggered by leptospires in nature are not effective in the elimination of this pathogen [2]. Accumulating evidence support a key role for CD4+ T cells in the acute and chronic stages of the infection in many bacterial diseases [3-5]. Immunity is specific for leptospiral types that have close
STUDIES ON MORPHOLOGICAL SUPERSTRUCTURE OF INACTIVATED RABIES VIRUS AND UNINACTIVATED RABIES VIRUS
狂犬病毒灭活前后形态结构的研究

Zhao Lin Li Aihua Sun Fulin,
赵林
,李爱华,孙富林

微生物学报 , 1994,
Abstract: Rabies is widely epidemic in human and animals. It is very dangerous disease at present. In 1919, semple has shown that antigenicity was not destroyed by chemicals. After that inactivated rabies vaccine was widely used in human being up to more than seventy years. But the afferration of morphological superstructure between inactivation and uninactivated, we have not see any report on the world.
OBSERVATIONS ON NEW CELL LINE OF Indian muntjac TRANSFORMED WITH MNNG
MNNG转化赤麂细胞株(KIZ—8401)的生长特性和核型观察

Liu Aihua Lin Shiying Xiong Xikun,
刘爱华
,林世英

动物学研究 , 1992,
Abstract: Lung fibroblast line of Indian muntjac (KIZ-7901) was transformed with MNNG (N-Methyl-N-nitro-N-nitrosoguanidine). The transformed Indian mu-ntjac: cells (KIZ-8401) possessed the new karyotype with cbromorsome number of 8 in male and polyploid. The morphology and cell cycle as well as mitotic index in MNNG-transformed muntjac cells were different from diploid cell line of Indian muntjac (KIZ-7901). Moreover, the MNNG-transformed muntjac cells possessed colony-forming capacity in vitro.
THE ESTABLISHMENT AND PRESERVATION OF LYMPHOCYTE STRAIN OF SOME RARE PRIMATES
几种珍稀灵长类淋巴细胞系的建立和保存

Liu Aihua Lin Shiying Shi Liming,
刘爱华
,林世英

动物学研究 , 1995,
Abstract: This is a report of the comparative study of the chromosomes in B95-8 cell transformed by EBV with that of the normal Sagninus oedipus studied by Hsu T. C. For the normal, the diploid chromosomes number is 2n= 46 and karyotype consists of three groups: A, 2 pairs submetacentric and 2 pairs of micrometacentric; B, 11 pairs of submetacentric; C, 7 pairs of telocentric. One large metacentric X-chromosome and a microtelocentric Y-chromosome can be differentiated easily.For the B95-8 cell which has been transformed through carrying EBV 2n = 47, the A and C group of the karyotype appeared as the same as the normal Sagninus oeaipus. But for the B group, there are 3 chromosomes without pairing (one large metacentric; one large submetacentric and one microtelocentric) with the exception of No.10 submetacentric. However, the lymphocyte of Rhinopitheus bieti, Rhimopitheus rotellanae, Hylobates hoolock, Presbytis francoisi, Presbytls phayrei crepusculus as well as Presbytis nemaeus transformed by EBV the diploid chromosome number, G-, C-banding and Ag-NORs are all consistent with that of their normal ones.
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