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Search Results: 1 - 10 of 15818 matches for " Adriana Mayumi Yano-Melo "
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Immature Embryo Rescue and in Vitro Development Evaluation of Intraspecific Hybrids from Brazilian Seedless Grapevine “Superior × Thompson” Clones  [PDF]
Eiryanne Fonseca de Menezes, Eliene Matos e Silva, Adriana Mayumi Yano-Melo, Patrícia Coelho de Souza Le?o, Natoniel Franklin de Melo
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.513209

The fruit production for export is an economically significant activity in the Valley of S?o Francisco River, especially in the irrigated lands of Petrolina-PE/Juazeiro-BA, Brazil. The development of new genetic material most suitable to the tropical climate and the demands of the consumer market have led to the selection of new seedless grapes cultivars. In this case, the use of the embryo rescue technique has produced satisfactory results for obtaining such materials, especially in the semiarid region. This study aimed to evaluate the in vitro development of intraspecific hybrids of grapevine (Vitis vinifera L.), derived from the rescue of immature embryos resultant from the crossing of “Superior Seedless” and “Thompson Seedless” Brazilian clones. To establish and develop the cultivation, the culture media was supplemented with 30 g/L sucrose, 0.1 g/L myo-inositol, 0.002 g/L glycine, 0.1 mg/L indoleacetic acid (IAA), 6.5 g/L of agar, adjusted pH to 5.7. The experiment was evaluated after 90 days. The variables measured were: number of nodes, number of leaves, plant height (cm), number of roots and length (cm) of the root system and internodes. The period of 60 days of in vitro culture of ovules resulted in the highest values of embryos (about 50%), as well as better characterized developmental stages with higher germination (47.3%). The three types of hybrid grapes evaluated in micropropagation showed very similar values of the measured parameters, even having originated from embryos of different developmental stages.

Filamentous fungi isolated from the rhizosphere of melon plants (Cucumis melo L. cv. Gold Mine) cultivated in soil with organic amendments
Coutinho, Flavia Paiva;Cavalcanti, Maria Auxiliadora de Queiroz;Yano-Melo, Adriana Mayumi;
Acta Botanica Brasilica , 2010, DOI: 10.1590/S0102-33062010000100032
Abstract: rhizosphere soil samples were collected in a semiarid area, in the region of the s?o francisco river valley, petrolina, pernambuco state, brazil, to study the diversity of filamentous fungi in a soil cultivated with melon (cucumis melo l. cv. gold mine) and receiving different organic amendments: treatment 1 (control, without organic compost); t2 (77% coconut fiber, 20% goat manure and 3% k2so4); t3 (10% ricinus communis leaves and stems, 50% pennisetum purpureum leaves and 40% goat manure); t4 (77% coconut fiber, 20% goat manure and 3% termophosphate); t5 (47% pennisetum purpureum leaves, 50% goat manure and 3% k2so4); and t6 (57% pennisetum purpureum leaves, 40% goat manure and 3% termophosphate). fungal isolation was carried out by the serial dilution technique to 1:1000. the sorensen index of similarity, frequency and distribution of the fungi were evaluated. seventy-eight species of filamentous fungi were isolated and identified, plus several basidiomycota (04) and mycelia sterilia (02). the predominant genera were aspergillus and penicillium, with 15 and 13 species, respectively. a greater number of species was found in the sowing period (49), and in relation to the organic fertilization, treatment 6 provided the greatest species diversity (43 species). most of the species are saprobes and only a few are considered to be potential pathogens on melon plants, such as fusarium oxysporum, f. solani and myrothecium roridum.
Phosphate-solubilizing fungi isolated from a semiarid area cultivated with melon (Cucumis melo L. cv. gold mine)
Coutinho, Flavia Paiva;Cavalcanti, Maria Auxiliadora de Queiroz;Yano-Melo, Adriana Mayumi;
Acta Botanica Brasilica , 2011, DOI: 10.1590/S0102-33062011000400020
Abstract: considering that little is known about the occurrence of phosphate-solubilizing fungi from areas cultivated with melon, the phosphate solubilization ability of filamentous fungi isolated in these areas was evaluated. three hundred and eighteen filamentous fungal isolates belonging to 23 genera were evaluated, besides aphyllophorales and mycelia sterilia. from those, 52 were able to solubilize p: aphyllophorales (2), aspergillus (34), penicillium (10) and rhizopus (6). these results will contribute to subsidizing further research regarding the capacity of these fungi to solubilize other sources of phosphate applied to the melon crop, as well as indicate the need for a screening program to select those with higher capacity and potential for solubilization.
Production and infectivity of inoculum of arbuscular mycorrhizal fungi multiplied in a substrate supplemented with Tris-HCl buffer
Silva, Fábio Sérgio Barbosa da;Yano-Melo, Adriana Mayumi;Maia, Leonor Costa;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000400030
Abstract: the effect of adding tris-hcl buffer on production and infectivity of amf inoculum was investigated. sporulation of glomus etunicatum, acaulospora longula and gigaspora albida was improved in solution with buffer. the infectivity of g. etunicatum increased after storage, what suggests that the inoculum of this isolate is benefited by storage.
Uso de vermicomposto favorece o crescimento de mudas de gravioleira (Annona muricata L. 'Morada') associadas a fungos micorrízicos arbusculares
Silva, Danielle Karla Alves da;Silva, Fábio Sérgio Barbosa da;Yano-Melo, Adriana Mayumi;Maia, Leonor Costa;
Acta Botanica Brasilica , 2008, DOI: 10.1590/S0102-33062008000300021
Abstract: annona muricata l. (soursop) easily adapts to irrigation in the semiarid northeast. the economically important fruits have high exportation potential, so production has been encouraged. the use of organic amendments, together with arbuscular mycorrhizal fungi (amf) may be useful for production of seedlings. however, the effect of such amendments on the symbiosis established between amf and soursop remains unknown. the effect of amf multiplied in a substrate with earthworm manure on the formation of a. muricata seedlings maintained in fertilized substrate was investigated. the experimental design was entirely random, in a factorial of 2×5 = 2 substrates (with or without 10% organic manure) and five inoculation treatments (acaulospora longula spain & schenck and gigaspora albida schenck & smith, produced in substrates with or without organic residue and an uninoculated control), with four replicates. after 102 days, dry mass of shoots and roots, height, growth rate, production of amf spores and glomalin, soil enzymatic activity, microbial respiration, total, arbuscular and hyphal colonization were evaluated. in general, seedling growth was stimulated by the inoculation with amf, but in the fertilized soil, growth of seedlings in symbiosis with g. albida was not benefited. the use of organic fertilizer stimulated mycorrhizal colonization, microbial respiration, enzymatic activity and glomalin production, but inhibited a. longula sporulation. the application of amf and organic manure may constitute an alternative for production of annona muricata, since it reduced by half the period for seedling formation, and can reduce by 75% the dose of fertilizer to be applied. however, the choice of amf compatible with the host is needed to assure positive results. the combination of both, amf and organic amendment, can also improve soil quality, thus contributing to sustainable production of seedlings of a. muricata and other fruit trees.
Fungos micorrízicos arbusculares e vermicomposto na aclimata??o de Alpinia purpurata (Viell.) Schum e Zingiber spectabile Griff. (Zingiberaceae)
Silva, Maryluce Albuquerque da;Silva, Fábio Sérgio Barbosa da;Yano-Melo, Adriana Mayumi;Melo, Natoniel Franklin de;Maia, Leonor Costa;
Acta Botanica Brasilica , 2006, DOI: 10.1590/S0102-33062006000200001
Abstract: to select amf and substrates that enhance production of micropropagated seedlings of alpinia purpurata and zingiber spectabile, tropical ornamental species, seedlings were inoculated with 1,500 infective propagules of gigaspora albida, glomus etunicatum and acaulospora longula, in pots containing: (a) soil; (b) soil and vermicompost (3:1); (c) soil, sand and vermicompost (2:2:1). the experimental design was completely randomized in a factorial of: four inoculation treatments (three with amf and uninoculated control), three substrates, with eight replicates for each plant species. after 70 (z. spectabile) and 100 (a. purpurata) days of inoculation, plant growth parameters such as height, leaf and offshoots number, dry mass of shoots and roots, leaf area, as well as mycorrhizal colonization, amf sporulation were evaluated. significant interactions were not observed between amf and substrates. in a. purpurata, the two substrates with vermicompost promoted growth, while in z. spectabile only the substrate with higher proportion of this compound enhanced plant growth. use of vermicompost reduced mycorrhizal colonization in both hosts and the sporulation of a. longula. during the acclimatization period, application of organic amendment is useful to induce formation of healthy seedlings of a. purpurata and z. spectabile but it may be harmful to the amf community.
Fungos micorrízicos arbusculares e rizobactérias promotoras de crescimento na aclimatiza??o de zingiber spectabile
Oliveira, Jo?o Ricardo Gon?alves de;Moraes, Thiago Alberto de Lima;Melo, Natoniel Franklin de;Yano-Melo, Adriana Mayumi;
Bragantia , 2010, DOI: 10.1590/S0006-87052010000300021
Abstract: beneficial microorganisms are important for survival and development of micropropagated plants. the objective of this work was to evaluate the potential of arbuscular mycorrhizal fungi (amf) and plant growth-promoting rhizobacteria (pgpr), isolated and/or combined (in dual inoculation) on acclimatization of zingiber spectabile griff. the experiment was carried out in a greenhouse using containers of 300 ml and dust from coconut shell (amafibra?) as substrate. the experimental design was completely randomized with two amf treatments [glomus etunicatum (ge) and the mixture of g. etunicatum and gigaspora margarita (ge/gm)], two treatments with pgpr inoculation [bacillus thuringiensis (hpf14) and b. pumilus (hps6)], four treatments combining these microorganisms [ge+hps6, ge+hpf14, ge/gm+hps6 and ge/gm+hpf14] and a control treatment (not inoculated), with eight replicates. after 90 days survival percentage, height, leaf area, fresh and dry biomass of shoots (fbs and dbs) and roots (fbr and dbr), mycorrhizal colonization and content of macro and micronutrients in the shoot were evaluated. co-inoculation (ge/gm+hps6) benefited significantly the mycorrhization when compared to the other treatments, resulting in higher fbs than that produced by hpf14 inoculation. although with 100% survival after the acclimatization period, the development of z. spectabile inoculated with hpf14 isolated and/or combined with amf is lower than the observed for control plants. the results suggest that the use of plant growth-promoting microorganisms should be done carefully, considering the cost/benefit of the application.
Soil biochemistry and microbial activity in vineyards under conventional and organic management at Northeast Brazil
Freitas, Nicácio de Oliveira;Yano-Melo, Adriana Mayumi;Silva, Fábio Sérgio Barbosa da;Melo, Natoniel Franklin de;Maia, Leonor Costa;
Scientia Agricola , 2011, DOI: 10.1590/S0103-90162011000200013
Abstract: the s?o francisco submedium valley is located at the brazilian semiarid region and is an important center for irrigated fruit growing. this region is responsible for 97% of the national exportation of table grapes, including seedless grapes. based on the fact that organic fertilization can improve soil quality, we compared the effects of conventional and organic soil management on microbial activity and mycorrhization of seedless grape crops. we measured glomerospores number, most probable number (mpn) of propagules, richness of arbuscular mycorrhizal fungi (amf) species, amf root colonization, ee-brsp production, carbon microbial biomass (c-mb), microbial respiration, fluorescein diacetate hydrolytic activity (fda) and metabolic coefficient (qco2). the organic management led to an increase in all variables with the exception of ee-brsp and qco2. mycorrhizal colonization increased from 4.7% in conventional crops to 15.9% in organic crops. spore number ranged from 4.1 to 12.4 per 50 g-1 soil in both management systems. the most probable number of amf propagules increased from 79 cm-3 soil in the conventional system to 110 cm-3 soil in the organic system. microbial carbon, co2 emission, and fda activity were increased by 100 to 200% in the organic crop. thirteen species of amf were identified, the majority in the organic cultivation system. acaulospora excavata, entrophospora infrequens, glomus sp.3 and scutellospora sp. were found only in the organically managed crop. s. gregaria was found only in the conventional crop. organically managed vineyards increased mycorrhization and general soil microbial activity.
O papel da comunidade de fungos micorrízicos arbusculares (FMA) autóctones no desenvolvimento de espécies vegetais nativas em área de dunas de restinga revegetadas no litoral do Estado da Paraíba
Oliveira, Jo?o Ricardo Gon?alves de;Souza, Renata Gomes de;Silva, Fábio Sérgio Barbosa da;Mendes, Alessandra Salviano Monteiro;Yano-Melo, Adriana Mayumi;
Brazilian Journal of Botany , 2009, DOI: 10.1590/S0100-84042009000400005
Abstract: association of arbuscular mycorrhizal fungi (amf) and plant roots constituted one of the most important ways to mitigate the impact of disturbed area. the aim of this work was to characterize the amf community in the soil from revegetated dune area and to analyze its influence on the native plant development. soils were collected in rainfall season in the revegetated area. glomalin-related soil protein production (grsp), glomerospores density and diversity were evaluated. the influence of amf autoctone community was determined for two native plants: tabebuia roseo-alba (ridl.) sandw. (peroba) and tocoyena selloana schum. (jenipapo-bravo). experimental design for each species was completely randomized with 10 replicates, two treatment - native soil (sn) and disinfested native soil (snd) - were tested. parameters evaluated were: height, shoot diameter, leaf number and area, shoot and root dry mass, nutrient contents, glomerospore density, mycorrhizal colonization and glomalin-related soil protein production (grsp). in dune revegetated soil was found 1 glomerospore g-1 soil and 1.20 ± 0.04 mg grsp g-1 soil, and six amf species were identified. native soil promoted more development to plant and fungi. mycorrhizal colonization in peroba was 80% and in jenipapo-bravo 60%. then, amf are present in revegetated area after mining contributing to growth of native plant species studied in greenhouse conditions, suggesting that their performance is affected by arbuscular mycorrhiza symbiosis.
Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates
Maia, Leonor C.;Yano-Melo, Adriana M.;
Brazilian Journal of Microbiology , 2001, DOI: 10.1590/S1517-83822001000400005
Abstract: the role of the substrate on germination of gigaspora albida schenck & smith was investigated. spores were desinfested with 0.5% sodium hypochlorite (20 min.) and placed on petri dishes over a millipore filter, with one of the following media: a- 1% water-agar; b- water-agar + aqueous extract of roots of panicum miliaceum l.; c- salt medium of murashige & skoog (ms) or sterilized sand; and incubated in the dark at room temperature (28oc ± 2). the experimental design was at random with four treatments and four replicates. germination was evaluated every 7 days until the 28th day. the water-agar medium was the most feasible for spore germination at the 7th day, followed by the ms medium at the 14th day. conversely, the sand and the root extract medium did not allow high germination. spores maintained in water-agar also presented longer germ tubes than spores in the other treatments. auxiliary cells were observed at the beginning of formation of hyphal branching in all treatments, however they were more numerous in the water-agar medium.
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