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Search Results: 1 - 10 of 419089 matches for " Adriana M.;Pol?nio "
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Associa??o do polimorfismo do gene da enzima conversora da angiotensina com dados ecocardiográficos em jovens normotensos filhos de hipertensos
Franken, Roberto Alexandre;Bellesso, Marcelo;Cavazin, Adriana M.;Polnio, Igor Bastos;Mattheucci, Euclides;Varga, José;
Revista da Associa??o Médica Brasileira , 2004, DOI: 10.1590/S0104-42302004000100037
Abstract: objectives: to evaluate angiotensin converting enzyme gene (ace) polymorphism with transthoracic bidimensional ecocardiogram of normotensive young medical students, siblings of hypertensive parents comparing them with those with normotensive parents. method: we had studied 80 normotensive youngs divided in two groups: hypertensive parents' normotensive young medical students 40 x normotensive parents'and normotensive medical students. exclusion criteria were hypertension, obesity, smoke, use of oral contraceptives, as well as those who use chronically drugs or the presence of any disease. the group has been enrolled between 1994 to 1996. 50 students made transthoracic bidimensional echocardiogram. the statistical analysis was done by ''t-student'' test. the evaluation of polymorphism ace gene was studied in 80 people in each step: 1) 5ml of blood in edta tube; 2) extraction of dna; 3) evaluation of dna concentration by eletrophoresis analyses; 4) polymerase chain reaction with primer of ace gene; 5) analysis of polymorphism ace gene by electrophoresis 6) statiscal analysis by chi-square test. results: the group of students with hypertesive parents presented thicker interventricular septum (7.82mm ± 0.69 against 7.38 mm ± 0,8, p < 0,05). on the other hand, we didn't find differences between the groups concerning ace gene genotype: students with hypertensive parents dd:42.5%, di: 37.5%, ii: 20% against students with normotensive parents : dd: 37.5%, di: 32.5%, ii: 30%, (p=0,58), in addition we also did not find differences concerning the alleles group of hypertensive parents: d: 61.25%, i: 38.75% versus normotensive parents: d: 53.75% , i: 46.25%, p=0,33. we divided these groups into two in relation to the mean thickness of interventricular septum and left ventricular mass and we did not find any difference: in students with hypertensive parents group septum > 7,82 mm: dd: 32%, di: 24%, ii: 20% x septum < 7,82 mm: dd: 8%, di: 12 %, ii: 4%, p=0,7) in normotensive paren
Associa o do polimorfismo do gene da enzima conversora da angiotensina com dados ecocardiográficos em jovens normotensos filhos de hipertensos
Franken Roberto Alexandre,Bellesso Marcelo,Cavazin Adriana M.,Polnio Igor Bastos
Revista da Associa??o Médica Brasileira , 2004,
Abstract: OBJETIVOS: Os autores objetivaram no presente estudo avaliar o polimorfismo da enzima conversora da angiotensina com dados do ecocardiograma de jovens estudantes de Medicina, filhos de hipertensos, comparados com jovens filhos de normotensos. MéTODOS: Foram estudados 80 jovens normotensos divididos em dois grupos: 40 filhos normotensos de pais hipertensos e 40 filhos normotensos de pais hipertensos. Critérios de exclus o foram hipertens o arterial, fumo, obesidade, uso de contraceptivos orais. Uso cr nico de medicamentos e presen a de qualquer doen a. Os alunos foram incluídos entre 1994 e 1996. Cinqüenta alunos foram submetidos a ecocardiograma transtoráxico. A análise estatística foi feita através do teste T de Student. A avalia o do polimorfismo do gene da enzima conversora da angiotensina foi feita nos 80 alunos conforme segue: 1) 5 ml de sangue em tubo contendo EDTA, 2) extra o do DNA, 3) medida da concentra o do DNA por eletroforese, 4) rea o em cadeia de polimerase com ''primer'' do gene da enzima conversora da angiotensina, 5) análise do polimorfismo do gene da enzima conversora da angiotensina através da eletroforese e 6) análise estatística através do teste do Qui-quadrado. RESULTADOS: O grupo de estudantes filhos de hipertensos mostraram maior espessura do septo interventricular (7,82mm+/-0,69 contra 7,38 +/- 0,8, p<0,05). Por outro lado n o encontramos diferen as entre os grupos em rela o ao genótipo do gene da enzima conversora. Filhos de hipertensos DD 42,5%, DI 37,5%, II 20% contra filhos de normotensos: DD 37,5%, DI 32,5%, II 30%, (p=0,58). N o encontramos mesmo diferen as quando considerados os alelos. O grupo com pais hipertensos D 61,25%, I 38,75%, contra grupo com pais normotensos D 53,75%, I 46,25% (p=0,33). Dividimos o grupo em dois, considerando a média da espessura do septo interventricular e a massa do ventrículo esquerdo e também n o encontramos diferen as: estudantes com pais hipertensos com septo > 7,82mm; DD 32%, DI 24%, II 20% contra septo < 7,82mm; DD 86%, DI 12%, II 4% (p=0,7). Naqueles com pais normotensos septo > 7,38mm: DD28%, DI12%, II 12%, contra septo < 7,38mm: DD16%, DI6%, II 16% (p=0,59). Em rela o à massa ventricular em filhos de pais hipertensos: massa > 131,52g: DD 20,69% DI 13,79%, II 6,9% contra massa < 131,52g: DD 24,24%, DI 17,24%, II 17,24% (p=0,72). No grupo de alunos filhos de pais normotensos massa > 117,11g: DD 30,43%, DI 8,7%, II 8,7% contra massa < 117,11g: DD 13,04%, DI 21,74%, II 17,39% (p=0,17) CONCLUS O: Encontramos diferen as entre a espessura do septo interventricular de estudantes normot
Detec??o do vírus da dengue em popula??es naturais de mosquitos
Bona,Ana Caroline Dalla; Twerdochlib,Adriana Lacerda; Navarro-Silva,Mário Ant?nio;
Boletín de Malariología y Salud Ambiental , 2011,
Abstract: dengue is caused by flavivirus which exhibits high genetic diversity, with four serotypes and various genotypes. the disease is endemic in most countries in the americas, and the female aedes (stegomyia) aegypti (linnaeus, 1762) are the only transmitters of epidemiological importance. a single infected mosquito remains so for the rest of his life and can infect multiple human hosts. for dengue virus detection in mosquitoes, the circulating serotype can be revealed or detect an entry of a new serotype in the region, without any ethical implications, and reproducible results. this review covers the techniques for detecting virus in mosquitoes, their advantages and limitations, as well as previous studies with natural populations of aedes aegypti.
Biologia floral e reprodutiva de Casearia grandiflora Camb. (Flacourtiaceae)
MACHADO, ADRIANA DE OLIVEIRA;OLIVEIRA, PAULO EUGêNIO A.M. DE;
Brazilian Journal of Botany , 2000, DOI: 10.1590/S0100-84042000000300004
Abstract: the floral biology of casearia grandiflora (flacourtiaceae) was studied in a remnant of mesophyllous forest in the parque do sabiá, uberlandia-mg. the species is an important understorey element in forests of this region, flowering during most of the year but more abundantly in march, april and may. flowers are pale-green, 7 mm across, in sessile axillary heads. they are odourless, one-day flowers, with 10 free stamens adenate to the corolla base. between the stamens there are receptacular appendages called inter-staminal disk lobes. the stamens and these structures form a cone around the pistil which retains the nectar. anthesis occurs irregularly but mainly early in the morning. nectar is relatively abundant (4 ml) and with 38% of sucrose equivalents. pollen is released during the warmest hours of the day, with high viability levels (96.6%). stigma is receptive at the same time. the most frequent visitor was the fly ornidia obesa (syrphidae), which was present on flowers most of the day, although other sporadic visitors such as meliponinae bees, butterflies and other unidentified flies were also observed. controlled hand pollinations showed the species is self-sterile and non-apomictic. however, pollen tubes were observed growing down to the ovary and penetrating the ovules in self-pollinated pistils, which suggest late-acting self sterility phenomena (post-fertilization) or inbreeding depression. self-sterility in c. grandiflora contrasts with autogamous breeding systems observed in most studied myophillous forest species with similar generalist flower morphology occurring in central brazil, but is similar to other neotropical woody species in the flacourtiaceae.
Monitoramento da audi??o de pacientes expostos à cisplatina
Garcia, Adriana P.;Iório, Maria Cecília M.;Petrilli, Ant?nio S.;
Revista Brasileira de Otorrinolaringologia , 2003, DOI: 10.1590/S0034-72992003000200011
Abstract: cisplatin is an antineoplasic drug, which has ototoxicity as a side effect. the goals of this paper were to evaluate the audiological behavior in osteosarcoma patients treated with cisplatin and to verify which evaluation method is the best for early detection of drug induced hearing loss. study design: clinical prospective. material and method: 13 patients, that received four cisplatin cycles of 120 mg/m2/cycle divided in two days (60 mg/m2/day), were evaluated prior to start of chemotherapy, prior to each scheduled course and at the end of treatment. it was performed the pure tone audiometry (250 to 18000 hz) and the transitory and distortion product otoacoustic emission (toae and dpoae). results: in the mean values, it was observed hearing loss, after 480 mg/m2 cumulative cisplatin dosage, beginning at 8 khz. at the individual values, it was observed that 15,3% had mild to moderate hearing loss beginning at 3khz, 15,3% beginning at 4 khz, 15,3% beginning at 6 khz and 15,3% beginning at 8 khz. toae did not show changes before the audiometry. dpoae showed smaller amplitude after the cycles of cisplatin, but this change happened together with the audiometry - not prior. conclusion: the high frequency audiometry was more efficient to detect early ototoxicity. toae and dpoae can be used as complement tests. all cisplatin exposed patients showed high frequency hearing loss, 30,6% showed hearing loss in important frequencies (3 and 4 khz) for speech comprehension.
Efeito da densidade na gaiola sobre o desempenho de poedeiras comerciais nas fases de cria, recria e produ??o
Pavan, Ana Cláudia;Garcia, Edivaldo Ant?nio;Móri, Cleusa;Pizzolante, Carla Cachoni;Piccinin, Adriana;
Revista Brasileira de Zootecnia , 2005, DOI: 10.1590/S1516-35982005000400029
Abstract: this trial was conducted to evaluate the effect of different stocking densities on performance of caged layers during the growing and laying periods and to verify the effects in the next phase. in the growing period (0-6 weeks), eight hundred and four chicks (isa brown ) were assigned to 100 x 80 x 35 cm cages, according to a completely randomized design, with four treatments (275.86, 250.00, 228.57, and 210.52 cm2 per bird) and four replicates. no significant effects of treatments on weight gain, feed intake, feed conversion and uniformity were observed during the growing period. in the 6-16 w period, 720 pullets were randomly assigned to 100 x 50 x 50 cm cages, according to a completely randomized design of 4 x 3 factorial arrangement (four densities from 0-6 weeks and three densities from 6-16 weeks), with different treatments (500.00, 416.67, and 357.14 cm2 per bird) and four replicates. no significant differences among treatments in the 6-16 w period on weight gain, feed intake, feed conversion and uniformity were observed. in the laying phase, 540 laying hens were randomly assigned to 100 x 45 x 45 cm cages, according to a completely randomized design of 3 x 3 factorial arrangement (three stocking densities in the 6-16 w periodand three caging densities in the laying phase), with different treatments (562.15, 450.00, and 375.00 cm2 per hen) and five replicates. significant effects of cage density on egg weight and feed intake were detected. no significant effects of cage density on bird performance in the growing and laying periods were observed.
Desempenho e rendimento de carca?a de quatro grupos genéticos de codornas para produ??o de carne
Móri, Cleusa;Garcia, Edivaldo Ant?nio;Pavan, Ana Cláudia;Piccinin, Adriana;Pizzolante, Carla Cachoni;
Revista Brasileira de Zootecnia , 2005, DOI: 10.1590/S1516-35982005000300019
Abstract: the study aimed to evaluate the performance and carcass yield of four quail genetic groups male and females. the birds were grown until 42 days old. a thousand and two hundred day old quails were distributed in a completely randomized design with four treatments (a, b, c and d) and five replicates of 60 birds each. the birds were weighed once a week in order to evaluate weight gain and feed consumption. mortalities were recorded daily. at 42 days, ten male quails from each replicate were identified and slaughtered to evaluate the yield of carcass and parts. in the period from 1 to 42 days, genetic group c showed higher values of body weight. group c showed the higher values for weight gain in the period from 1 to 42 days. no differences concerning the results of feed consumption, feed efficiency, mortality, body weight, carcass weight, carcass yield and breast percentage were observed among the groups. genetic group d showed higher values for wings, legs and breast meat percentage. it was concluded that there are differences of performance and carcass and parts yield among the quail genetic groups selected for meat production presented on brazilian market.
Genetic diversity of dengue virus serotypes 1 and 2 in the State of Paraná, Brazil, based on a fragment of the capsid/premembrane junction region
Bona, Ana Caroline Dalla;Twerdochlib, Adriana Lacerda;Navarro-Silva, Mário Ant?nio;
Revista da Sociedade Brasileira de Medicina Tropical , 2012, DOI: 10.1590/S0037-86822012000300003
Abstract: introduction: the precise identification of the genetic variants of the dengue virus is important to understand its dispersion and virulence patterns and to identify the strains responsible for epidemic outbreaks. this study investigated the genetic variants of the capsid-premembrane junction region fragment in the dengue virus serotypes 1 and 2 (denv1-2). methods: samples from 11 municipalities in the state of paraná, brazil, were provided by the central laboratory of paraná. they were isolated from the cell culture line c6/36 (aedes albopictus) and were positive for indirect immunofluorescence. ribonucleic acid (rna) extracted from these samples was submitted to the reverse transcription polymerase chain reaction (rt-pcr) and nested pcr. results: rt-pcr revealed that 4 of the samples were co-infected with both serotypes. the isolated denv-1 sequences were 95-100% similar to the sequences of other serotype 1 strains deposited in genbank. similarly, the isolated denv-2 sequences were 98-100% similar to other serotype 2 sequences in genbank. according to our neighbor-joining tree, all strains obtained in this study belonged to genotype v of denv-1. the denv-2 strains, by contrast, belonged to the american/asian genotypes. conclusions: the monitoring of circulating strains is an important tool to detect the migration of virus subtypes involved in dengue epidemics.
Monitoramento da audi o de pacientes expostos à cisplatina
Garcia Adriana P.,Iório Maria Cecília M.,Petrilli Ant?nio S.
Revista Brasileira de Otorrinolaringologia , 2003,
Abstract: A cisplatina é um agente quimioterápico que apresenta dentre seus efeitos colaterais a ototoxicidade. Este estudo teve como objetivos avaliar a audi o de pacientes portadores de osteossarcoma expostos à cisplatina e verificar qual o método de investiga o mais adequado para identificar precocemente as altera es auditivas induzidas por drogas ototóxicas. FORMA DE ESTUDO: Clínico prospectivo. MATERIAL E MéTODO: 13 indivíduos portadores de osteossarcoma que receberam quatro ciclos de cisplatina de 120 mg/m2/ciclo, fracionados em dois dias de aplica o (60 mg/m2/dia), foram submetidos à avalia o audiológica - audiometria tonal liminar (250 a 18000 Hz) e emiss es otoacústicas transitórias (EOAT) e por produto de distor o (EOAPD) - antes do início do tratamento e após cada ciclo de cisplatina. RESULTADOS: Observou-se, nos valores médios, perda auditiva após a dose cumulativa de 480 mg/m2 a partir de 8 kHz. Quanto aos valores individuais, 15,3% dos pacientes apresentaram perda auditiva de grau leve a moderado a partir de 3 kHz, 15,3% a partir de 4 kHz, 15,3% a partir de 6 kHz e 15,3% a partir de 8 kHz. N o foi observada redu o na amplitude das EOAT precocemente à altera o dos limiares nas altas freqüências. Houve redu o da amplitude das EOAPD concomitante ao aumento do limiar de audibilidade. CONCLUS O: A audiometria de altas freqüências é mais efetiva na detec o precoce da perda auditiva induzida por cisplatina. As EOAT e EOAPD podem ser usadas como complemento à avalia o audiométrica. Todos os pacientes expostos à cisplatina têm perda auditiva nas altas freqüências, e destes, 30,6% tem perda auditiva a partir das freqüências de 3 e 4 kHz consideradas importantes para a compreens o da fala.
Biologia floral e reprodutiva de Casearia grandiflora Camb. (Flacourtiaceae)
MACHADO ADRIANA DE OLIVEIRA,OLIVEIRA PAULO EUGêNIO A.M. DE
Brazilian Journal of Botany , 2000,
Abstract: A biologia de reprodu o de Casearia grandiflora (Flacourtiaceae) foi estudada em um remanescente de mata mesófila do Parque do Sabiá, Uberlandia - MG. A espécie é importante no sub-bosque de matas da regi o, florescendo durante quase todo o ano, mas com maior abundancia nos meses de mar o, abril e maio. As flores s o branco-esverdeadas com cerca de 7 mm de diametro, dispostas em capítulos sésseis axilares. N o apresentam odor perceptível e duram apenas 1 dia. Possuem 10 estames férteis livres entre si e unidos à corola na base. Entre os estames existem estruturas pilosas originárias do receptáculo floral. Os estames e estas estruturas formam um cone em torno do pistilo, onde se acumula o néctar. A antese ocorre de forma irregular, principalmente no início da manh . O néctar é relativamente abundante (4 miL) e com concentra o média de 38% de equivalentes de sacarose. O pólen é liberado nas horas mais quentes do dia, com alta viabilidade (96,6%). Neste horário também ocorre a receptividade estigmática. O visitante mais freqüente foi a mosca Ornidia obesa (Syrphidae), que visita as flores durante quase todo o dia, embora tenham sido observados também outros visitantes casuais como abelhas Meliponinae, borboletas e outras moscas n o identificadas. Poliniza es controladas mostraram que a planta é auto-incompatível e n o apomítica. No entanto, foram observados tubos polínicos crescendo até o ovário e penetrando os óvulos em flores autopolinizadas, sugerindo a ocorrência de fen menos de auto-esterilidade de a o tardia (pós-fertiliza o) ou depress o de endogamia. A auto-esterilidade em C. grandiflora contrasta com a autogamia observada na maior parte das plantas miófilas e de flores generalistas ocorrendo nas florestas do Brasil central, mas é semelhante aos sistemas de reprodu o de outras Flacourtiaceae lenhosas estudadas em florestas Neotropicais.
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