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Search Results: 1 - 10 of 3614 matches for " AM;Zamuner "
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Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom
Barbosa, AM;Villaverde, AB;Guimar?es-Sousa, L;Soares, AM;Zamuner, SF;Cogo, JC;Zamuner, SR;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2010, DOI: 10.1590/S1678-91992010000300014
Abstract: the prominent myotoxic effects induced by bothrops jararacussu crude venom are due, in part, to its polycationic myotoxins, bthtx-i and bthtx-ii. both myotoxins have a phospholipase a2 structure: bthtx-ii is an active enzyme asp-49 pla2, while bthtx-i is a lys-49 pla2 devoid of enzymatic activity. in this study, the effect of low-level laser therapy (lllt), 685 nm laser at a dose of 4.2 j/cm2 on edema formation, leukocyte influx and myonecrosis caused by bthtx-i and bthtx-ii, isolated from bothrops jararacussu snake venom, was analyzed. bthtx-i and bthtx-ii caused a significant edema formation, a prominent leukocyte infiltrate composed predominantly by neutrophils and myonecrosis in envenomed gastrocnemius muscle. lllt significantly reduced the edema formation, neutrophil accumulation and myonecrosis induced by both myotoxins 24 hours after the injection. lllt reduced the myonecrosis caused by bthtx-i and bthtx-ii, respectively, by 60 and 43%; the edema formation, by 41 and 60.7%; and the leukocyte influx, by 57.5 and 51.6%. in conclusion, lllt significantly reduced the effect of these snake toxins on the inflammatory response and myonecrosis. these results suggest that lllt should be considered a potential therapeutic approach for treatment of local effects of bothrops species venom.
Anti-inflammatory activity of Blutaparon portulacoides ethanolic extract against the inflammatory reaction induced by Bothrops jararacussu venom and isolated myotoxins BthTX-I and II
Pereira, IC;Barbosa, AM;Salvador, MJ;Soares, AM;Ribeiro, W;Cogo, JC;Zamuner, SR;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2009, DOI: 10.1590/S1678-91992009000300013
Abstract: this article reports the anti-inflammatory effect of blutaparon portulacoides (b. portulacoides), specifically the ethanolic extract of its aerial parts, on the edema formation and leukocyte influx caused by bothrops jararacussu (b. jararacussu) snake venom and bothropstoxin-i and ii (bthtx-i and ii) isolated from this venom as an alternative treatment for bothrops snakebites. the anti-inflammatory effect of b. portulacoides ethanolic extract was compared with an animal group pretreated with dexamethasone. b. portulacoides ethanolic extract significantly inhibited paw edema induced by b. jararacussu venom and by bthtx-i and ii. also, results demonstrated that the extract caused a reduction of the leukocyte influx induced by bthtx-i. however, the extract was not capable of inhibiting the leukocyte influx induced by the venom and by bthtx-ii. in conclusion, these results suggest that the ethanolic extract of this plant possess components able to inhibit or inactivate toxins present in b. jararacussu venom, including its myotoxins, responsible for the edema formation. however, the leukocyte migration caused by the venom and bthtx-ii was not inhibited by the plant, probably due to the different mechanisms involved in the edema formation and leukocyte influx. this is the first report of b. portulacoides extract as anti-inflammatory against snake venoms and isolated toxins.
Agathoxylon matildense n. sp., le?o araucariaceo del Bosque Petrificado del cerro Madre e Hija, Formación La Matilde (Jurásico medio), provincia de Santa Cruz, Argentina
Zamuner,Alba B.; Falaschi,Paula;
Ameghiniana , 2005,
Abstract: the anatomy of a specimen from the middle jurassic of the cerro madre e hija area, la matilde formation, santa cruz province is presented. the anatomical characteristics observed allowed us to classify this specimen as a new member of the genus agathoxylon : a . matildense n. sp. distinct growth rings are observed in transversal sections while radial views show uniseriate tracheid pitting, contiguous and predominantly circular in shape, cross-fields are cupressoid with 4-5 pits irregularly arranged. rays are homogeneous and very low (1-4 cells hight). wood characters, its relationships with living related taxa and the associated fossil flora allow us to consider this wood as an araucariaceae member. agathoxylon matildense n. sp. probably grew under warm-temperate and humid climate with rainfall seasonality.
Impact of Feedlot on Soil Phosphorus Concentration  [PDF]
Nicolás Wyngaard, Liliana Picone, Cecilia Videla, Ester Zamuner, Néstor Maceira
Journal of Environmental Protection (JEP) , 2011, DOI: 10.4236/jep.2011.23031
Abstract: Raising cattle in feedlots is becoming more common in Argentina, but there is little information available about the effects of this practice on soil phosphorus (P) dynamics. In this study, concentrations of water and Bray -1 extractable soil P were quantified in a feedlot (upper and lower slope positions in the feedlot pen), runoff area and pasture during 2006 - 2008. The feedlot showed higher P concentrations in all P forms, soluble reactive P, soluble nonreactive, total soluble P, and Bray-P1 when compared to runoff area and pasture. Most of the total soluble P was soluble reactive P from the soil in the feedlot and the runoff area, while soluble nonreactive P predominated in the pasture. Concentrations of total soluble P were elevated in the feedlot at the soil surface, ranging on average from 158.71 to 245.86 mg?P?kg?1, and had a rapid decrease within the first 20 cm and remained relatively low, about 25.53 - 27.33 mg?P?kg?1. The evidence that total soluble P concentration in the feedlot was significantly (p < 0.05) higher than that registered in the pasture at 20 - 40 and 40 - 60 cm soil depth suggests a potential transfer of P through the soil from the surface. Although the feedlot had a moderate increase of 23.05 to 24.55 mg?P?kg?1 from the background concentration, it may represent a long-term source of increased nutrient loading to groundwater. Soil extractable Bray-1 P in the top 0 - 2.5 cm fluctuated from 659.26 to 45.36 mg?P?kg?1 in the feedlot and pasture, respectively. The relationship between soil P extracted by test and TSP was linear, and correlation coefficient was r2 = 0.95.
Cell adhesion molecules involved in the leukocyte recruitment induced by venom of the snake Bothrops jararaca
Stella R. Zamuner,Catarina F. P. Teixeira
Mediators of Inflammation , 2002, DOI: 10.1080/0962935021000051548
Abstract: It has been shown that Bothrops jararaca venom (BjV) induces a significant leukocyte accumulation, mainly neutrophils, at the local of tissue damage. Therefore, the role of the adhesion molecules intercellular adhesion molecule-1 (ICAM-1), LECAM-1, CD18, leukocyte function-associated antigen-1 (LFA-1) and platelet endothelial cell adhesion molecule-1 (PECAM-1) on the BjV-induced neutrophil accumulation and the correlation with release of LTB4, TXA2, tumor necrosis factor-α, interleukin (IL)-1 and IL-6 have been investigated. Anti-mouse LECAM-1, LFA-1, ICAM-1 and PECAM-1 monoclonal antibody injection resulted in a reduction of 42%, 80%, 66% and 67%, respectively, of neutrophil accumulation induced by BjV (250 μg/kg, intraperitoneal) injection in male mice compared with isotype-matched control injected animals. The anti-mouse CD18 monoclonal antibody had no significant effect on venom-induced neutrophil accumulation. Concentrations of LTB4, TXA2, IL-6 and TNF-α were significant increased in the peritoneal exudates of animals injected with venom, whereas no increment in IL-1 was detected. This results suggest that ICAM-1, LECAM-1, LFA-1 and PECAM-1, but not CD18, adhesion molecules are involved in the recruitment of neutrophils into the inflammatory site induced by BjV. This is the first in vivo evidence that snake venom is able to up-regulate the expression of adhesion molecules by both leukocytes and endothelial cells. This venom effect may be indirect, probably through the release of the inflammatory mediators evidenced in the present study.
Efecto de extractos acuosos de dos meliaceas sobre Meloidogyne incognita (Nematoda, meloidogynidae)
Mareggiani, Graciela;Zamuner, Norma;Angarola, Gisella;
Revista latinoamericana de química , 2010,
Abstract: the bioactivity of six aqueous extracts 10% and 30% (p/v), containing terpenoids from melia azedarachand trichilia glauca (meliaceae) in their chemical constitution, was evaluated against the pest meloidogyne incognita (nematoda, meloidogynidae). results show that the natural products assayed had a good nematicidal activity, because they had moderate lethal effects (mortality: 23-46.2%, depending upon treatment). these data show that the interesting properties of both meliaceae could be used in orchard nematode management, in replacement of synthetic nematicides.
Produtividade de madeira de liquidambar (Liquidambar styraciflua L.) de diferentes procedências, em Quedas do Igua?u, PR
Shimizu, Jarbas Yukio;Spir, Ivana Helena Zamuner;
Revista árvore , 2004, DOI: 10.1590/S0100-67622004000400003
Abstract: a combined provenance/progeny trial with sweetgum (liquidambar styraciflua l.) from mexico and central america was established in quedas do igua?u, in the southwest of the state of paraná, in order to assess wood productivity and genetic variability. the trial was established on a sloping site and arranged in a compact family block design, with the progenies planted into 5-tree linear plots. at eleven years of age, the mean yield of the fastest growing provenances (all from central america, from latitudes no higher than 15o n) was higher than 40 m3/ha.year. significant variations were detected among progenies within gomez farias, las lajas, los alpes, and monte bello provenances. however, among these, only los alpes and las lajas provenances offer opportunities for genetic improvement of wood production through selection among their progenies since the mean productivity in the others were low. although no significant variation was detected among progenies within tactic and yucul provenances, their inclusion in breeding populations is important, given their high mean productivity and the opportunity to increase population sizes for further selections based on other traits such as stem form and wood quality.
Nitrogen dynamics in a feedlot soil
Wyngaard,N; Videla,C; Picone,L; Zamuner,E; Maceira,N;
Journal of soil science and plant nutrition , 2012, DOI: 10.4067/S0718-95162012005000016
Abstract: the feedlot system (fl) generates a high input of sewage causing imbalances in the cycling process of nitrogen (n) as well as a potential negative impact on the environment. the objective of this study was to deepen into the dynamics of n in a fl's soil so as to provide tools to reduce its environmental impact. the study was conducted in a fl, located in balcarce county (buenos aires, argentina). soil samples were taken between november 2006 and may 2007 in a control pasture, in the fl-high (upper slope position) and fl-low (lower slope position) of the fl, and in the runoff area (ra). urease activity and concentrations of ammonium-n, nitrate-n and urea-n were evaluated in the soil up to 60 cm depth. urea-n values were always low (6 mg kg-1), due to the high urease activity measured (438 mg kg-1). ammonium-n concentration was highest in fl-low, reaching a value of 65.5 mg kg-1 at 40-60 cm soil depth. nitrate-n concentration was highest in fl-high for all depths, with a maximum concentration of 97.4 mg kg-1 in the 0-10 cm layer; at 40-60 cm depth, nitrate-n concentration in fl was 31.98 mg kg-1, higher than that in pasture (1.21 mg kg-1). there were no differences in nitrate-n concentration between pasture and ra; hence, it can be inferred that there was no transport of nitrate by surface water flow from the fl to ra. however, the potential for groundwater contamination via nitrate, a mobile soil constituent, should be studied.
Una nueva Equisetaceae fértil de la Formación La Matilde, Jurásico Medio, Argentina
Falaschi,Paula; Zamuner,Alba B.; Foix,Nicolás;
Ameghiniana , 2009,
Abstract: here we describe impressions of vegetative and fertile organs of equisetaceae coming from the monumento natural bosques petrificados locality, la matilde formation (middle jurassic), santa cruz province. they are very frequent in tuffaceous laminated levels with assymmetrical ripple marks, micro-crossbedding and bioturbations associated with shallow lacustrine environment. stems are herbaceous, grass-like and have extremely small dimensions: diameter 1,3 mm, internodes 0,9 cm long, cross section hexagonal with 6 carinae and valeculae and 6 leaves per node fused into a leaf sheath. no branching was observed. the material is mostly vegetative, but three specimens with mature strobili show hexagonal, verticilate sporangiophores. the observed characters allow us to assign them to a new species of equisetites: e. minimus n.sp. the stems lie parallel to the bedding planes or cross them suggesting life position. they probably grew in dense, monotypic communities, with an essentially vegetative reproduction.
Análisis de los anillos de crecimiento del bosque fósil de Víctor Szlápelis, Terciario inferior del Chubut, Argentina
Brea,Mariana; Matheos,Sergio; Zamuner,Alba; Ganuza,Daniel;
Ameghiniana , 2005,
Abstract: growth rings are described in gymnosperm woods of the victor szlápelis forest, salamanca formation, lower paleocene of the chubut province, argentina. this forest is composed by a large number of petrified trunks of parautocthonous provenance. the presence of trees with more than 1 m in diameter suggests an exuberant growth and good environmental conditions for tree development. associated sediments suggests that fossil trees were developed in a stable environment related with fluvial to deltaic characteristics. tree-ring methods are used to study the growth characteristics of these gymnosperms. wood anatomy and characters associated to the growth records indicate that climate was warm-temperate and humid, with a dry summer and a wet spring.
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