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Bacillus thuringiensis isolates entomopathogenic for Culex quinquefasciatus (Diptera: Culicidae) and Anticarsia gemmatalis (Lepidoptera: Noctuidae)
Gobatto, V.;Giani, SG.;Camassola, M.;Dillon, AJP.;Specht, A.;Barros, NM.;
Brazilian Journal of Biology , 2010, DOI: 10.1590/S1519-69842010000500018
Abstract: samples of the bacillus thuringiensis (bt) were collected from soil and insects. eight isolates were selected from rural soil, 15 from urban soil and 11 from insects. these were evaluated for entomopathogenicity against larvae of anticarsia gemmatalis and culex quinquefasciatus. the pathogenicity tests showed that a higher percentage of isolates were active against a. gemmatalis (60%) compared to c. quinquefasciatus (31%). probit analysis (lc50) indicated that against a. gemmatalis four of the isolates presented values similar to the reference strain against a. gemmatalis, while against c. quinquefasciatus one isolate showed an lc50 similar to the reference strain (ips-82). sds-page characterisation of two isolates showed a 27 kda protein fraction related to the bt subspecies israelensis cytolytic toxin (cyt) gene. one 130 kda protein, possibly related to the bt crystal inclusions (cry1) gene, was identified in the other two isolates, which were more toxic for lepidoptera; another isolate presented a protein of 100 kda. some new local bt isolates had similar lc50 probit values to the reference strains.
Survey of infectious laryngotracheitis outbreak in layer hens and differential diagnosis with other respiratory pathogens
Chacón, JLV;Brand?o, PEB;Villarreal, LYB;Gama, NM;Ferreira, AJP;
Revista Brasileira de Ciência Avícola , 2007, DOI: 10.1590/S1516-635X2007000100009
Abstract: trachea, lung, and conjunctive samples from 51 commercial layer farms from bastos region, s?o paulo, brazil, were submitted to nested-pcr and virus isolation in spf chicken embryos for ilt diagnosis. this region experienced an outbreak characterized by respiratory signs, decrease in egg production and increased mortality. out of the 51 tested field samples, 23 were positive for ilt by nested-pcr, 22 were positive after the virus isolation, and 24 were positive when both techniques were used. newcastle disease virus, avian pneumovirus, or mycoplasma gallisepticum were not detected. infectious bronchitis virus was detected in one farm and mycoplasma synoviae was detected in eight farms. the high incidence of infectious laryngotracheitis virus (iltv) detection, the high correlation between the observed clinical signs and the iltv detection, and the results of differential diagnosis demonstrated that iltv was the causative agent of the outbreak of respiratory disease observed in bastos region, s?o paulo, brazil.
Randomized double blind trial of ciprofloxacin prophylaxis during induction treatment in childhood acute lymphoblastic leukemia in the WK-ALL protocol in Indonesia
Widjajanto PH, Sumadiono S, Cloos J, Purwanto I, Sutaryo S, Veerman AJP
Journal of Blood Medicine , 2013, DOI: http://dx.doi.org/10.2147/JBM.S33906
Abstract: ndomized double blind trial of ciprofloxacin prophylaxis during induction treatment in childhood acute lymphoblastic leukemia in the WK-ALL protocol in Indonesia Original Research (473) Total Article Views Authors: Widjajanto PH, Sumadiono S, Cloos J, Purwanto I, Sutaryo S, Veerman AJP Published Date February 2013 Volume 2013:4 Pages 1 - 9 DOI: http://dx.doi.org/10.2147/JBM.S33906 Received: 16 May 2012 Accepted: 31 July 2012 Published: 01 February 2013 Pudjo H Widjajanto,1 Sumadiono Sumadiono,1 Jacqueline Cloos,2,3 Ignatius Purwanto,1 Sutaryo Sutaryo,1 Anjo JP Veerman1,2 1Pediatric Hematology and Oncology Division, Department of Pediatrics, Dr Sardjito Hospital, Medical Faculty, Universitas Gadjah Mada, Yogyakarta, Indonesia; 2Pediatric Oncology/Hematology Division, Department of Pediatrics, 3Department of Hematology, VU University Medical Center, Amsterdam, The Netherlands Objectives: Toxic death is a big problem in the treatment of childhood acute lymphoblastic leukemia (ALL), especially in low-income countries. Studies of ciprofloxacin as single agent prophylaxis vary widely in success rate. We conducted a double-blind, randomized study to test the effects of ciprofloxacin monotherapy as prophylaxis for sepsis and death in induction treatment of the Indonesian childhood ALL protocol. Methods: Patients were randomized to the ciprofloxacin arm (n = 58) and to the placebo arm (n = 52). Oral ciprofloxacin monotherapy or oral placebo was administered twice a day. All events during induction were recorded: toxic death, abandonment, resistant disease, and complete remission rate. Results: Of 110 patients enrolled in this study, 79 (71.8%) achieved CR. In comparison to the placebo arm, the ciprofloxacin arm had lower nadir of absolute neutrophil count during induction with median of 62 (range: 5–884) versus 270 (range: 14–25,480) × 109 cells/L (P > 0.01), greater risks for experiencing fever (50.0% versus 32.7%, P = 0.07), clinical sepsis (50.0% versus 38.5%, P = 0.22), and death (18.9% versus 5.8%, P = 0.05). Conclusion: In our setting, a reduced intensity protocol in a low-income situation, the data warn against using ciprofloxacin prophylaxis during induction treatment. A lower nadir of neutrophil count and higher mortality were found in the ciprofloxacin group.
Rotavirus detection and isolation from chickens with or without symptoms
Villarreal, LYB;Uliana, G;Valenzuela, C;Chacón, JLV;Saidenberg, ABS;Sanches, AA;Brand?o, PE;Jerez, JA;Ferreira, AJP;
Revista Brasileira de Ciência Avícola , 2006, DOI: 10.1590/S1516-635X2006000300009
Abstract: rotaviruses have been identified as one of the main etiological agents of diarrhea and enteritis in mammals, including humans, and in avian species. few studies have been published about enteric viruses in brazilian poultry, including those related to rotavirus infection. such studies demonstrate significant occurrence and the importance of enteric viruses in poultry presenting intestinal problems. enteric viruses are the primary cause of injuries to the gut, allowing other agents, especially bacteria, to attach, to penetrate, and to replicate in the enteric tissue, leading to further damage. the aim of the present study was to detect rotavirus in the intestinal contents of layers and broilers by polyacrylamide gel electrophoresis (page) and virus isolation in ma-104 cell culture. a total of 45.3% of all samples were positive to rotavirus; rotavirus frequencies were 48.7% in samples from flocks with diarrhea, 46.4% in flocks with delayed growth, and 30% in asymptomatic flocks. it was possible to isolate rotavirus in ma-104 cells from the nine rotavirus-positive randomly chosen samples. these results indicate that rotavirus may have an important role in pathogenesis of enteric disease.
Detection and molecular characterization of infectious laryngotracheitis virus in laying hens in Brazil
Villarreal, LYB;Brand?o, PEB;Chacón, JLV;Doretto Junior, L;Ito, N;Gama, NS;Ishizuka, MM;Luchese, A;Buchala, F;Astolfi-Ferreira, CS;Ferreira, AJP;
Revista Brasileira de Ciência Avícola , 2004, DOI: 10.1590/S1516-635X2004000400011
Abstract: avian infectious laryngotracheitis, caused by infectious laryngotracheitis virus (iltv), has been reported for decades in brazilian laying and broiler flocks. more recently, outbreaks have occurred in s?o paulo state. this study reports the application of pcr and dna sequencing targeted to the p32 gene of iltv using laying chicken samples from bastos, s?o paulo, brazil. three out of four field samples were positive by pcr. dna sequencing of two samples evidenced homology of the amplified fragments with the p32 gene of iltv. the results definitely confirmed the presence of iltv in the birds during the outbreak. further studies are needed to establish the sources of infection and to determine whether the detected virus was originated from vaccine or field virus strains.
Identification of turkey astrovirus and turkey coronavirus in an outbreak of Poult Enteritis and Mortality Syndrome
Villarreal, LYB;Assayag, MS;Brand?o, PE;Chacón, JLV;Bunger, AND;Astolfi-Ferreira, CS;Gomes, CR;Jones, RC;Ferreira, AJP;
Revista Brasileira de Ciência Avícola , 2006, DOI: 10.1590/S1516-635X2006000200010
Abstract: this article reports a survey on turkey astrovirus (tastv) and turkey coronavirus (tcov) infections with rt-pcr in 17 turkey flocks affected by acute enteritis and two apparently normal turkey flocks located in the southeastern region of brazil by pcr (tastv and tcov). seven out of the 17 affected flocks were positive for tastv and 14 for tcov, with seven co-infections. in one of the two apparently normal flocks, a tastv-tcov co-infection was found. although a definitive association of these agents and the signs can not be made, the implications of these findings are discussed.
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